Rroxscaffold_2G00124110

Alginate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000532
Physical Location & Seq
Reverse (-)
58221993 .. 58224320
2328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00124110.1

Sequence Viewer

Length: 846 bp
ATGAAAATGGGGAGGATCGGTCCATGCAACGCCGGAAATCGTGTTTTCCGGCCGGGTCGGGTCGGCGAAGGCCGGGATGCCTTCGATCCAAACTTCGGGCGGCGGCGGTGCAAGGGAGCACCGGAGGGAGCTTGGGCGGCTGCGTCCGAGGGTGGTGAGGGCCGGTCCGGTGCAGGGAGGTGGCCGGAGGGTGGCGAAAATTCGGGTCGGGTCGGCCGGTTGCACGGTCTTCACCAGTCAGCCTTGGCTTGGAGTTGGGGACCTGATGTTCCTCCTACACAGGGCTTTGTCTCCCTCCCTTTCAACCGATCCTACTACCATATTCAGAAACCTTATGATGTGCCTGAAGACCAGCGCTACTGCTTCATCGATGGAGTTCATAAATGTTGGGTCTACTCCACAGACAAACCTCACACTACCACCAGCCAAACCCTGCCTCGCACTGAAATTGCTATACAAGGATACGGTTACTCTTCTGGTGTGTGGGAATTTGAAGCATATGCGTATGTGCCAAATGGAACATCAGGGGTCTGCATAATGCAAGTGTTTGGAGCAACTGCTCCTCATGCCTCAACTCTGATGTTGAGGGTCTACAATGGTTCACTCACCTACTACAGGGCTCCAGTACTGGTTCCAAACATCTACGATAAATGGTTCAAGATCAATGTGGTTCACGACGTTGATGCTGCGAAAGTCAGAGTCTTCATTGACGGCTGTCTCAAAATCGAGGCAGATGGTCGTGGAGGAACGTCTCATACTTTCAAATGTGGAGTGTATGCCCAGATGAATGACTCTACTTACATGGAGTCTCGCTGGAAAGACATCAAAGTTTTAAGGAAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

281

Amino Acids

31.21

Weight (kDa)

9.04

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 104 - 277 5.4e-26 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 393, 591
AciI CCGC 4 cut(s) 100, 103, 106, 137
AclWI GGATC 3 cut(s) 23, 80, 303
AcoI YGGCCR 3 cut(s) 50, 182, 214
AcsI RAATTY 2 cut(s) 199, 488
AcuI CTGAAG 1 cut(s) 366
AfaI GTAC 1 cut(s) 627
AfeI AGCGCT 1 cut(s) 356
AfiI CCNNNNNNNGG 6 cut(s) 95, 174, 191, 249, 281, 615
AgsI TTSAA 4 cut(s) 304, 494, 658, 763
AloI GAACNNNNNNTCC 2 cut(s) 252, 284
AluBI AGCT 1 cut(s) 131
AluI AGCT 1 cut(s) 131
Alw21I GWGCWC 1 cut(s) 121
Alw26I GTCTC 4 cut(s) 295, 722, 756, 813
AlwI GGATC 3 cut(s) 23, 80, 303
Aor51HI AGCGCT 1 cut(s) 356
AoxI GGCC 5 cut(s) 50, 70, 160, 182, 214
ApeKI GCWGC 2 cut(s) 140, 686
ApoI RAATTY 2 cut(s) 199, 488
AspLEI GCGC 1 cut(s) 357
AspS9I GGNCC 4 cut(s) 20, 160, 165, 260
AsuC2I CCSGG 2 cut(s) 54, 74
AsuHPI GGTGA 3 cut(s) 167, 224, 598
AvaII GGWCC 3 cut(s) 20, 165, 260
BanII GRGCYC 1 cut(s) 622
BbsI GAAGAC 3 cut(s) 221, 354, 694
Bbv12I GWGCWC 1 cut(s) 121
BbvI GCAGC 2 cut(s) 127, 673
BccI CCATC 2 cut(s) 365, 728
BceAI ACGGC 1 cut(s) 727
BcgI CGANNNNNNTGC 2 cut(s) 665, 699
BciVI GTATCC 1 cut(s) 455
BcnI CCSGG 2 cut(s) 54, 74
BcoDI GTCTC 4 cut(s) 295, 722, 756, 813
BfmI CTRYAG 1 cut(s) 613
BfoI RGCGCY 1 cut(s) 358
BfuI GTATCC 1 cut(s) 455
BisI GCNGC 5 cut(s) 101, 104, 138, 141, 687
BlsI GCNGC 5 cut(s) 102, 105, 139, 142, 688
BmcAI AGTACT 1 cut(s) 627
Bme1390I CCNGG 2 cut(s) 54, 74
Bme18I GGWCC 3 cut(s) 20, 165, 260
BmgT120I GGNCC 4 cut(s) 20, 160, 165, 260
BmiI GGNNCC 3 cut(s) 261, 621, 633
BmrFI CCNGG 2 cut(s) 54, 74
BmsI GCATC 2 cut(s) 67, 673
BoxI GACNNNNGTC 1 cut(s) 714
BpiI GAAGAC 3 cut(s) 221, 354, 694
BpmI CTGGAG 1 cut(s) 606
BpuMI CCSGG 2 cut(s) 54, 74
Bsa29I ATCGAT 1 cut(s) 369
BsaJI CCNNGG 2 cut(s) 147, 243
BsaWI WCCGGW 2 cut(s) 121, 167
BsaXI ACNNNNNCTCC 1 cut(s) 34
Bsc4I CCNNNNNNNGG 6 cut(s) 95, 174, 191, 249, 281, 615
Bse118I RCCGGY 2 cut(s) 162, 216
Bse1I ACTGG 3 cut(s) 235, 623, 633
BseCI ATCGAT 1 cut(s) 369
BseDI CCNNGG 2 cut(s) 147, 243
BseGI GGATG 1 cut(s) 82
BseLI CCNNNNNNNGG 6 cut(s) 95, 174, 191, 249, 281, 615
BseNI ACTGG 3 cut(s) 235, 623, 633
BseRI GAGGAG 1 cut(s) 552
BseX3I CGGCCG 2 cut(s) 50, 214
BseXI GCAGC 2 cut(s) 127, 673
BsgI GTGCAG 1 cut(s) 192
Bsh1285I CGRYCG 2 cut(s) 53, 217
BshFI GGCC 5 cut(s) 52, 72, 162, 184, 216
BshVI ATCGAT 1 cut(s) 369
BsiEI CGRYCG 2 cut(s) 53, 217
BsiHKAI GWGCWC 1 cut(s) 121
BsiSI CCGG 9 cut(s) 33, 49, 53, 73, 122, 163, 168, 185, 217
BslFI GGGAC 1 cut(s) 273
BslI CCNNNNNNNGG 6 cut(s) 95, 174, 191, 249, 281, 615
BsmAI GTCTC 4 cut(s) 295, 722, 756, 813
BsmBI CGTCTC 1 cut(s) 756
BsmFI GGGAC 1 cut(s) 273
BsnI GGCC 5 cut(s) 52, 72, 162, 184, 216
Bsp1286I GDGCHC 2 cut(s) 121, 622
Bsp143I GATC 4 cut(s) 15, 85, 308, 660
BspACI CCGC 4 cut(s) 100, 103, 106, 137
BspANI GGCC 5 cut(s) 52, 72, 162, 184, 216
BspDI ATCGAT 1 cut(s) 369
BspLI GGNNCC 3 cut(s) 261, 621, 633
BspPI GGATC 3 cut(s) 23, 80, 303
BsrFI RCCGGY 2 cut(s) 162, 216
BsrI ACTGG 3 cut(s) 235, 623, 633
BssAI RCCGGY 2 cut(s) 162, 216
BssECI CCNNGG 2 cut(s) 147, 243
BssMI GATC 4 cut(s) 15, 85, 308, 660
BssT1I CCWWGG 1 cut(s) 243
Bst4CI ACNGT 2 cut(s) 227, 467
Bst6I CTCTTC 1 cut(s) 478
BstENI CCTNNNNNAGG 1 cut(s) 613
BstF5I GGATG 1 cut(s) 82
BstH2I RGCGCY 1 cut(s) 358
BstHHI GCGC 1 cut(s) 357
BstKTI GATC 4 cut(s) 18, 88, 311, 663
BstMAI GTCTC 4 cut(s) 295, 722, 756, 813
BstMBI GATC 4 cut(s) 15, 85, 308, 660
BstMCI CGRYCG 2 cut(s) 53, 217
BstMWI GCNNNNNNNGC 2 cut(s) 137, 566
BstPAI GACNNNNGTC 1 cut(s) 714
BstSCI CCNGG 2 cut(s) 52, 72
BstSFI CTRYAG 1 cut(s) 613
BstV1I GCAGC 2 cut(s) 127, 673
BstV2I GAAGAC 3 cut(s) 221, 354, 694
BstZI CGGCCG 2 cut(s) 50, 214
Bsu15I ATCGAT 1 cut(s) 369
BsuI GTATCC 1 cut(s) 455
BsuRI GGCC 5 cut(s) 52, 72, 162, 184, 216
BsuTUI ATCGAT 1 cut(s) 369
BtsCI GGATG 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 441
CfoI GCGC 1 cut(s) 357
Cfr10I RCCGGY 2 cut(s) 162, 216
Cfr13I GGNCC 4 cut(s) 20, 160, 165, 260
ClaI ATCGAT 1 cut(s) 369
CpoI CGGWCCG 1 cut(s) 165
CseI GACGC 1 cut(s) 132
Csp6I GTAC 1 cut(s) 626
CspI CGGWCCG 1 cut(s) 165
CviAII CATG 3 cut(s) 24, 566, 802
CviQI GTAC 1 cut(s) 626
DpnI GATC 4 cut(s) 17, 87, 310, 662
DpnII GATC 4 cut(s) 15, 85, 308, 660
EaeI YGGCCR 3 cut(s) 50, 182, 214
EagI CGGCCG 2 cut(s) 50, 214
Eam1104I CTCTTC 1 cut(s) 478
EarI CTCTTC 1 cut(s) 478
EclXI CGGCCG 2 cut(s) 50, 214
Eco130I CCWWGG 1 cut(s) 243
Eco24I GRGCYC 1 cut(s) 622
Eco47I GGWCC 3 cut(s) 20, 165, 260
Eco47III AGCGCT 1 cut(s) 356
Eco52I CGGCCG 2 cut(s) 50, 214
Eco57I CTGAAG 1 cut(s) 366
EcoNI CCTNNNNNAGG 1 cut(s) 613
EcoO109I RGGNCCY 1 cut(s) 260
EcoT14I CCWWGG 1 cut(s) 243
EcoT38I GRGCYC 1 cut(s) 622
ErhI CCWWGG 1 cut(s) 243
Esp3I CGTCTC 1 cut(s) 756
FaeI CATG 3 cut(s) 27, 569, 805
FaqI GGGAC 1 cut(s) 273
FatI CATG 3 cut(s) 23, 565, 801
FauNDI CATATG 1 cut(s) 499
FblI GTMKAC 2 cut(s) 393, 591
Fnu4HI GCNGC 5 cut(s) 101, 104, 138, 141, 687
FokI GGATG 1 cut(s) 89
FriOI GRGCYC 1 cut(s) 622
Fsp4HI GCNGC 5 cut(s) 101, 104, 138, 141, 687
GlaI GCGC 1 cut(s) 356
GluI GCNGC 5 cut(s) 101, 104, 138, 141, 687
GsuI CTGGAG 1 cut(s) 606
HaeII RGCGCY 1 cut(s) 358
HaeIII GGCC 5 cut(s) 52, 72, 162, 184, 216
HapII CCGG 9 cut(s) 33, 49, 53, 73, 122, 163, 168, 185, 217
HgaI GACGC 1 cut(s) 132
HhaI GCGC 1 cut(s) 357
Hin1II CATG 3 cut(s) 27, 569, 805
Hin6I GCGC 1 cut(s) 355
HinP1I GCGC 1 cut(s) 355
HinfI GANTC 3 cut(s) 699, 791, 806
HpaII CCGG 9 cut(s) 33, 49, 53, 73, 122, 163, 168, 185, 217
HphI GGTGA 3 cut(s) 167, 224, 598
Hpy166II GTNNAC 4 cut(s) 394, 592, 602, 673
Hpy188I TCNGA 4 cut(s) 148, 327, 579, 698
Hpy188III TCNNGA 2 cut(s) 658, 674
Hpy8I GTNNAC 4 cut(s) 394, 592, 602, 673
Hpy99I CGWCG 1 cut(s) 680
HpyAV CCTTC 2 cut(s) 62, 91
HpyCH4III ACNGT 2 cut(s) 227, 467
HpyCH4IV ACGT 2 cut(s) 678, 749
HpyCH4V TGCA 6 cut(s) 27, 111, 173, 223, 534, 541
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 566
HpySE526I ACGT 2 cut(s) 678, 749
Hsp92II CATG 3 cut(s) 27, 569, 805
HspAI GCGC 1 cut(s) 355
Kzo9I GATC 4 cut(s) 15, 85, 308, 660
LmnI GCTCC 5 cut(s) 116, 128, 551, 565, 625
Lsp1109I GCAGC 2 cut(s) 127, 673
LweI GCATC 2 cut(s) 67, 673
MaeII ACGT 2 cut(s) 678, 749
MaeIII GTNAC 1 cut(s) 467
MalI GATC 4 cut(s) 17, 87, 310, 662
MboI GATC 4 cut(s) 15, 85, 308, 660
MboII GAAGA 4 cut(s) 221, 359, 465, 694
MhlI GDGCHC 2 cut(s) 121, 622
MluCI AATT 3 cut(s) 199, 447, 488
MlyI GAGTC 3 cut(s) 708, 785, 815
MseI TTAA 1 cut(s) 833
MspI CCGG 9 cut(s) 33, 49, 53, 73, 122, 163, 168, 185, 217
MspR9I CCNGG 2 cut(s) 54, 74
MwoI GCNNNNNNNGC 2 cut(s) 137, 566
NciI CCSGG 2 cut(s) 54, 74
NdeI CATATG 1 cut(s) 499
NdeII GATC 4 cut(s) 15, 85, 308, 660
NlaIII CATG 3 cut(s) 27, 569, 805
NlaIV GGNNCC 3 cut(s) 261, 621, 633
PkrI GCNGC 5 cut(s) 102, 105, 139, 142, 688
PleI GAGTC 3 cut(s) 707, 785, 814
PpsI GAGTC 3 cut(s) 707, 785, 814
PpuMI RGGWCCY 1 cut(s) 260
PshAI GACNNNNGTC 1 cut(s) 714
Psp5II RGGWCCY 1 cut(s) 260
PspN4I GGNNCC 3 cut(s) 261, 621, 633
PspPI GGNCC 4 cut(s) 20, 160, 165, 260
PspPPI RGGWCCY 1 cut(s) 260
PsrI GAACNNNNNNTAC 2 cut(s) 739, 771
RsaI GTAC 1 cut(s) 627
RsaNI GTAC 1 cut(s) 626
Rsr2I CGGWCCG 1 cut(s) 165
RsrII CGGWCCG 1 cut(s) 165
SaqAI TTAA 1 cut(s) 833
SatI GCNGC 5 cut(s) 101, 104, 138, 141, 687
Sau3AI GATC 4 cut(s) 15, 85, 308, 660
Sau96I GGNCC 4 cut(s) 20, 160, 165, 260
ScaI AGTACT 1 cut(s) 627
SchI GAGTC 3 cut(s) 708, 785, 815
ScrFI CCNGG 2 cut(s) 54, 74
SduI GDGCHC 2 cut(s) 121, 622
SetI ASST 8 cut(s) 133, 182, 265, 334, 412, 611, 681, 752
SfaNI GCATC 2 cut(s) 67, 673
SfcI CTRYAG 1 cut(s) 613
SinI GGWCC 3 cut(s) 20, 165, 260
Sse9I AATT 3 cut(s) 199, 447, 488
SsiI CCGC 4 cut(s) 100, 103, 106, 137
StyD4I CCNGG 2 cut(s) 52, 72
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 2 cut(s) 227, 467
TaiI ACGT 2 cut(s) 681, 752
TaqI TCGA 3 cut(s) 84, 369, 726
TaqII GACCGA 1 cut(s) 8
TasI AATT 3 cut(s) 199, 447, 488
TatI WGTACW 1 cut(s) 625
TauI GCSGC 3 cut(s) 103, 106, 140
Tru1I TTAA 1 cut(s) 833
Tru9I TTAA 1 cut(s) 833
TscAI CASTG 1 cut(s) 448
TseI GCWGC 2 cut(s) 140, 686
TspDTI ATGAA 5 cut(s) 17, 355, 368, 694, 800
TspRI CASTG 1 cut(s) 448
VpaK11BI GGWCC 3 cut(s) 20, 165, 260
XagI CCTNNNNNAGG 1 cut(s) 613
XapI RAATTY 2 cut(s) 199, 488
XmiI GTMKAC 2 cut(s) 393, 591
ZrmI AGTACT 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.