Prupe.6G161900_v2.0.a1

Alginate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000532
Physical Location & Seq
Reverse (-)
15104791 .. 15106340
1550 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G161900.2

Sequence Viewer

Length: 681 bp
ATGGCATATGACTCTTCTTTTCAGTTGCTAGGTTTCTTCTGCTTGATGATCAGCTTTCTTCAGCCCATTCTACTCCAAGCCTTAGCTTGGGGACCTGATGTTCCTACACAAGGCTTTGTCTCCCTCCCTTTTAACCGATCATACTACCATATTCAGAAGCCTTATGATGTCCCCGAAGAGCAGCGCTACAGCTTCGTCGATGGAATTCATAAATGTTGGGTCTACTCTACAGACAAACCTCACACTACTACCAGCCAAACCATGCCCCGTACTGAGATTGCCATACAAGGATACAATTACTCTTCCGGGGTGTGGGAATTTGAAGCATATGGCTATGTACCATATGGGACATCAAGTGTGTGCATAATGCAAGTGTTTGGAGCAAGTGCTCCTCATGCCACAACTCTAATGCTTGGGGTTTACAATGGTTCACTCATGTACTACACGGGTCCAGTGTTGGTACCAAACATCTATGACAAATGGTTCAAGTTGAATGTGATTCACGATGTTGAAGCTGCAAAAGTGAAGGTTTACATTGATGGATGTCTCAAATTAGAGGCAGATGGTCGTGGAGGCACATCTCATGCATTCAAATGTGGTGTATACGCCCAGCATAATGACTCCTTTTACATGGAGTCTCGTTGGAAACACATCCAAGTTTCAAGAAAGTGTAGACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

227

Amino Acids

25.73

Weight (kDa)

7.06

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 460
AccB1I GGYRCC 1 cut(s) 460
AccI GTMKAC 3 cut(s) 222, 603, 673
AcsI RAATTY 2 cut(s) 204, 317
AcuI CTGAAG 1 cut(s) 44
AfaI GTAC 4 cut(s) 271, 339, 440, 462
AfeI AGCGCT 1 cut(s) 185
AfiI CCNNNNNNNGG 3 cut(s) 87, 110, 312
AgsI TTSAA 6 cut(s) 323, 487, 493, 512, 592, 663
AloI GAACNNNNNNTCC 2 cut(s) 84, 116
AluBI AGCT 4 cut(s) 54, 86, 192, 515
AluI AGCT 4 cut(s) 54, 86, 192, 515
Alw21I GWGCWC 1 cut(s) 391
Alw26I GTCTC 3 cut(s) 124, 551, 642
Aor51HI AGCGCT 1 cut(s) 185
ApeKI GCWGC 2 cut(s) 181, 515
ApoI RAATTY 2 cut(s) 204, 317
Asp718I GGTACC 1 cut(s) 460
AspLEI GCGC 1 cut(s) 186
AspS9I GGNCC 2 cut(s) 92, 449
AsuC2I CCSGG 1 cut(s) 307
AvaII GGWCC 2 cut(s) 92, 449
BanI GGYRCC 1 cut(s) 460
Bbv12I GWGCWC 1 cut(s) 391
BbvI GCAGC 2 cut(s) 193, 502
BccI CCATC 3 cut(s) 194, 533, 557
BciVI GTATCC 1 cut(s) 284
BclI TGATCA 1 cut(s) 48
BcnI CCSGG 1 cut(s) 307
BcoDI GTCTC 3 cut(s) 124, 551, 642
BfaI CTAG 1 cut(s) 29
BfmI CTRYAG 2 cut(s) 187, 228
BfoI RGCGCY 1 cut(s) 187
BfuI GTATCC 1 cut(s) 284
BisI GCNGC 2 cut(s) 182, 516
BlsI GCNGC 2 cut(s) 183, 517
Bme1390I CCNGG 1 cut(s) 307
Bme18I GGWCC 2 cut(s) 92, 449
BmgT120I GGNCC 2 cut(s) 92, 449
BmiI GGNNCC 3 cut(s) 93, 450, 462
BmrFI CCNGG 1 cut(s) 307
Bpu10I CCTNAGC 1 cut(s) 82
BpuMI CCSGG 1 cut(s) 307
BsaJI CCNNGG 1 cut(s) 306
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 110, 312
Bse1I ACTGG 1 cut(s) 452
BseDI CCNNGG 1 cut(s) 306
BseGI GGATG 2 cut(s) 548, 651
BseLI CCNNNNNNNGG 3 cut(s) 87, 110, 312
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 452
BseRI GAGGAG 1 cut(s) 381
BseXI GCAGC 2 cut(s) 193, 502
BseYI CCCAGC 1 cut(s) 609
BshNI GGYRCC 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 391
BsiSI CCGG 1 cut(s) 306
BslFI GGGAC 3 cut(s) 105, 155, 361
BslI CCNNNNNNNGG 3 cut(s) 87, 110, 312
BsmAI GTCTC 3 cut(s) 124, 551, 642
BsmFI GGGAC 3 cut(s) 105, 155, 361
BsmI GAATGC 1 cut(s) 587
Bsp1286I GDGCHC 1 cut(s) 391
Bsp143I GATC 2 cut(s) 48, 137
BspCNI CTCAG 1 cut(s) 265
BspLI GGNNCC 3 cut(s) 93, 450, 462
BspQI GCTCTTC 1 cut(s) 171
BspT107I GGYRCC 1 cut(s) 460
BsrI ACTGG 1 cut(s) 452
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 2 cut(s) 48, 137
BssNAI GTATAC 1 cut(s) 604
Bst1107I GTATAC 1 cut(s) 604
Bst6I CTCTTC 3 cut(s) 19, 171, 307
BstDEI CTNAG 2 cut(s) 82, 273
BstENI CCTNNNNNAGG 1 cut(s) 108
BstF5I GGATG 2 cut(s) 548, 651
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 2 cut(s) 51, 140
BstMAI GTCTC 3 cut(s) 124, 551, 642
BstMBI GATC 2 cut(s) 48, 137
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstSCI CCNGG 1 cut(s) 305
BstSFI CTRYAG 2 cut(s) 187, 228
BstV1I GCAGC 2 cut(s) 193, 502
BstZ17I GTATAC 1 cut(s) 604
BsuI GTATCC 1 cut(s) 284
BtsCI GGATG 2 cut(s) 548, 651
BtsIMutI CAGTG 1 cut(s) 459
CfoI GCGC 1 cut(s) 186
Cfr13I GGNCC 2 cut(s) 92, 449
Csp6I GTAC 4 cut(s) 270, 338, 439, 461
CviAII CATG 5 cut(s) 262, 395, 436, 584, 631
CviQI GTAC 4 cut(s) 270, 338, 439, 461
DdeI CTNAG 2 cut(s) 82, 273
DpnI GATC 2 cut(s) 50, 139
DpnII GATC 2 cut(s) 48, 137
Eam1104I CTCTTC 3 cut(s) 19, 171, 307
EarI CTCTTC 3 cut(s) 19, 171, 307
Eco47I GGWCC 2 cut(s) 92, 449
Eco47III AGCGCT 1 cut(s) 185
Eco57I CTGAAG 1 cut(s) 44
EcoNI CCTNNNNNAGG 1 cut(s) 108
EcoO109I RGGNCCY 1 cut(s) 92
EcoRI GAATTC 1 cut(s) 204
EcoT22I ATGCAT 1 cut(s) 589
FaeI CATG 5 cut(s) 265, 398, 439, 587, 634
FaqI GGGAC 3 cut(s) 105, 155, 361
FatI CATG 5 cut(s) 261, 394, 435, 583, 630
FauNDI CATATG 3 cut(s) 7, 328, 343
FbaI TGATCA 1 cut(s) 48
FblI GTMKAC 3 cut(s) 222, 603, 673
Fnu4HI GCNGC 2 cut(s) 182, 516
FokI GGATG 2 cut(s) 555, 638
Fsp4HI GCNGC 2 cut(s) 182, 516
FspBI CTAG 1 cut(s) 29
GlaI GCGC 1 cut(s) 185
GluI GCNGC 2 cut(s) 182, 516
GsaI CCCAGC 1 cut(s) 613
HaeII RGCGCY 1 cut(s) 187
HapII CCGG 1 cut(s) 306
HhaI GCGC 1 cut(s) 186
Hin1II CATG 5 cut(s) 265, 398, 439, 587, 634
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HinfI GANTC 4 cut(s) 11, 499, 620, 635
HpaII CCGG 1 cut(s) 306
Hpy166II GTNNAC 6 cut(s) 223, 421, 431, 532, 604, 674
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 2 cut(s) 503, 663
Hpy8I GTNNAC 6 cut(s) 223, 421, 431, 532, 604, 674
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 1 cut(s) 520
HpyCH4V TGCA 4 cut(s) 363, 370, 518, 587
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 2 cut(s) 82, 273
Hsp92II CATG 5 cut(s) 265, 398, 439, 587, 634
HspAI GCGC 1 cut(s) 184
KpnI GGTACC 1 cut(s) 464
Ksp22I TGATCA 1 cut(s) 48
Kzo9I GATC 2 cut(s) 48, 137
LguI GCTCTTC 1 cut(s) 171
LmnI GCTCC 2 cut(s) 380, 394
LpnPI CCDG 5 cut(s) 108, 265, 319, 465, 623
Lsp1109I GCAGC 2 cut(s) 193, 502
MaeI CTAG 1 cut(s) 29
MalI GATC 2 cut(s) 50, 139
MboI GATC 2 cut(s) 48, 137
MboII GAAGA 5 cut(s) 6, 28, 50, 188, 294
MhlI GDGCHC 1 cut(s) 391
MluCI AATT 4 cut(s) 204, 295, 317, 551
MlyI GAGTC 3 cut(s) 5, 614, 644
MmeI TCCRAC 1 cut(s) 623
MnlI CCTC 5 cut(s) 134, 249, 402, 550, 566
Mph1103I ATGCAT 1 cut(s) 589
MseI TTAA 1 cut(s) 132
MslI CAYNNNNRTG 1 cut(s) 592
MspI CCGG 1 cut(s) 306
MspR9I CCNGG 1 cut(s) 307
Mva1269I GAATGC 1 cut(s) 587
MwoI GCNNNNNNNGC 1 cut(s) 395
NciI CCSGG 1 cut(s) 307
NdeI CATATG 3 cut(s) 7, 328, 343
NdeII GATC 2 cut(s) 48, 137
NlaIII CATG 5 cut(s) 265, 398, 439, 587, 634
NlaIV GGNNCC 3 cut(s) 93, 450, 462
NsiI ATGCAT 1 cut(s) 589
PciSI GCTCTTC 1 cut(s) 171
PctI GAATGC 1 cut(s) 587
PfeI GAWTC 1 cut(s) 499
PkrI GCNGC 2 cut(s) 183, 517
PleI GAGTC 3 cut(s) 5, 614, 643
PpsI GAGTC 3 cut(s) 5, 614, 643
PpuMI RGGWCCY 1 cut(s) 92
Psp5II RGGWCCY 1 cut(s) 92
PspFI CCCAGC 1 cut(s) 609
PspN4I GGNNCC 3 cut(s) 93, 450, 462
PspPI GGNCC 2 cut(s) 92, 449
PspPPI RGGWCCY 1 cut(s) 92
RsaI GTAC 4 cut(s) 271, 339, 440, 462
RsaNI GTAC 4 cut(s) 270, 338, 439, 461
RseI CAYNNNNRTG 1 cut(s) 592
SapI GCTCTTC 1 cut(s) 171
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 2 cut(s) 182, 516
Sau3AI GATC 2 cut(s) 48, 137
Sau96I GGNCC 2 cut(s) 92, 449
SchI GAGTC 3 cut(s) 5, 614, 644
ScrFI CCNGG 1 cut(s) 307
SduI GDGCHC 1 cut(s) 391
SetI ASST 9 cut(s) 34, 56, 88, 97, 194, 241, 517, 531, 679
SfcI CTRYAG 2 cut(s) 187, 228
SinI GGWCC 2 cut(s) 92, 449
SmiMI CAYNNNNRTG 1 cut(s) 592
Sse9I AATT 4 cut(s) 204, 295, 317, 551
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 1 cut(s) 305
TaqI TCGA 1 cut(s) 198
TasI AATT 4 cut(s) 204, 295, 317, 551
TatI WGTACW 1 cut(s) 438
TfiI GAWTC 1 cut(s) 499
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TscAI CASTG 1 cut(s) 459
TseI GCWGC 2 cut(s) 181, 515
TspDTI ATGAA 1 cut(s) 197
TspRI CASTG 1 cut(s) 459
VpaK11BI GGWCC 2 cut(s) 92, 449
XagI CCTNNNNNAGG 1 cut(s) 108
XapI RAATTY 2 cut(s) 204, 317
XmiI GTMKAC 3 cut(s) 222, 603, 673
XspI CTAG 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.