pycom01g03170

Alginate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000532
Physical Location & Seq
Forward (+)
2505343 .. 2511051
5709 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g03170.6

Sequence Viewer

Length: 4095 bp
ATGACTAGCAACCTTCACTGCTTTCTAGCATCAAATTCATTTTTGAATAAAACAAGTAAGCAAATTGCATTCACGATTCACAAATTCATAACTGGATTTAATCAATTCATATTGCATATAAAATCATGGCTTCAAATACACCTAAAACGCCTAGCGAATCAACTTGAATGCCCTACACGTTCAAGCTCCTTTCTCTTTTTTCTCCTTGCTGCAGCACATAGGTTAAGGGATGATTTTGGATGTGTTTCTGGGTTAGGAATGGATTTGGGTTGGTATGAATGTATGGGAATGGTGGAGAATGAGTGTAGGGTCCGGCAGAGTAGTGGGATGAATTGGGGGTGTTTAAAGATGGTGTATGGCGTGACTGATCGATCCAAAGGGTGCGGCCAAGGTTTATTAAAATATTTTATTAATGTCAAAGGATTTTTTTATTCTAAAACCACTAGGAAAAAGACTAAGTCAAAGTCAGAAATTAAAAGGAAAGGGAATATAGGGTGCGGCAAAGGTGATTTGGTGCAGAAAATAGGAAAGGGAAGGTCTCCCTTGTGCGGCAAGGAAGGAGAATGGAAGGGGAAAGGGAAATATGGTGCGGCAAGGGTTAGAATGGTGCAAGGAACCTTTGGTTTGTGTCCTAGAATGCATAGGAGACCTTCAATGTTGAAACCTTGGCTTAACTTTCCTACTTCAAGTAAGAATCCTCATTTGAGTAGGAATCATTCTTTGATTAGGAATCCTTTCTTGATTAGGAATCCTAACATCGTCAAGTCTTCAATTTCGTCCATTCCTTTTGCACCTTATTGCTTCATAAGGCCTATTGACCTACACACACACGAAAATAGAAACAAGCTAATTAAGTCGCATAAATACGCTCCTATCATATACCCCCAGCGTGTCATTAGGCTGAGTTGCTACATTTGTCGGAACAAAAGTAGTTTTTATATTCGGCAATCTATGTGGGGTAGTCCTGTCTCATCAGAGAGCCTTGTTAATAGCTTTGTTGTGTGGGAAATTGTGCAATCGCTCGGTCAGGAGCCTTTCTACTTCTTCTTGAATTTGCCTTTGTTAGGGTCTAGGCTGCTCTTCCACGTGTTCGGCTCGTCTATGCTATTGCTGCGGTGGGTGTGGTATGTTCTTAGCAGGCGAGTGGGTAACTCGCTGACTTGTGTTGCCTACTCTAATGTGAGATGGAGGATGCTCCTTACGAGCTTTGCCACAAATGAACACTTTGCCTGGAAGGTTGTTCATGTTGATTATTGGAGTGTGGCCCCAACCGTGAGTGTATGTTTGTCCGTGGGCTTAACTGAAAGTTCGGGAGTGTACTCTGTTAGAACGCTCGGTTCATGGCTGGTTAAGAGGCTGCTTACCGGGACGTTGGTGGAGAGGTTCGTCTGCCCTTGTCCTTCTTTAGGACACCTCGTTCGGGGCATGTTGGATCTCGGTTCGTTTGTTGTTCACCATTTAGATTGGAAGGGCTTGGAAGGAATATGCCTCATTGAGCAGTGGAAGTGTGGTAGACTCCGGGAGCGAGATTTGAATTGGGAAATGTCAAATCCGCAGAGAAATGTGGTGAAAGTGCCCCCGGCTCGATGGTTAATCAGGATGGTTGAGACAGCTGGGCCACGGGAGGGGCTAGGCCGCAAGAGTAGGCTAGGCCGCGAGAGTAGGCTAGGCCACGAAAATGGGCTGCTCGACGGGAGCAGGCTGGGCCACAGGAGTGGGTTGCTTGGCGTAGATGCACGCGGGTGCTCGGCATGGGCTCGGCTTGGCAACGCATTGGCCGCCTTGGATGGCACAGCTCGGACCGTGGTGGTGACATTGTGGACCTCACCGCGGGTGGCTACCATGGTAGCCACTGCGGTGGTTCCCGTGGTTGAAACTCGTGGTGGTGGTGGCACTCCACCTATTGTCACATTTTGTCTCGTGGATCGTTTTTCTAAATTTCTAACCATTGTACTTCTCTTTTACGTTTTATCAAAGAATTTTTTAATTAACAAACTGCTTAATTTTATCTCTCTGGTGGTTTGTTTGGAACTTTGGTGCTTGTTACACCCATCCAGGGGTATAGTGAACACCGAGCTAATCATGGCATGGCATTCAGGGCGGAAGCTTGTAGGGGGAAGTGACGTAGGAGGGGTGAAAGAGACGCAGGAGATGCTTGACTTCTGTGCCAAGCACAACATTACCTCAGACATCGAGCTGATTCGAATGGATTACATTAATACAGCAATGGAACGACTTGCCAAATCGGATGTCAGGTATCGGTTTGTGATCGATACGGCTATGGTTTTGAGTTTTTTCGGTGGAGGTAACATATCCTGGACACTTGGTGGTTGTGCCGGTCTAGTTATATTGATCTTCCGCCATTTTCGGTTTGATTATATTTCTTGTCTCTTTACTGCTTGGATTTTGTTGATATGCCTTAGTTTTTCTTTTTCTGTTTCTTATTTTCTAGGTCAAACCAAAACTCCTATAGTTTTTGTTACTTTAACATCTCTAATGAAACAAAGTGTCGGTATCGTCAAACAGTCTGACATGTTACGTTATGAGACGTTTATTACTACACTTGGGCTATTTCTACAGTGGTTACATATTTGGAAAGAGTATTACGCCCTGCAGATTTCTAGGTCTCTTGTTGTTTGCTTCTGTTTATGGAGATCCTTAATATTGGTTAACAATTTAACAGTCACCTTTCTTGTTTTCTTTTCAAATGATTTTTTCTCATCAGAGTACATATATTTAATTTGCCATAACAAGTATCACAGATATATATTTTTGTTCACTGTTGAGACCATTGTTCCACATCAAGAAACTAAAAAGCAAAACTTATTGGGTTTTGCATTGGACGTTCTAATTCCATTATCCCTTCCAAAGACTATCAATATCATTCATATCTCATTTACATTTTCGAGATGTTTTGAGATCCCATTTATTTATCTCTTCAAATGGCGGGACAAACTTCTTAGGGCATCTCCATTGGAGAGATTTATGGAAGATGAAAATGGGAGTTCTACTCTAATGGCGAACTTTACTTTTAAAGTTCAATTCAACAATACACTTGGTTCCACAAAATTGTGGAGGAATTTGCTTAAGATCAGAATTGTTCATGGTATAAATCACTTTGTAAAGATCATAAATATAAAAACATATGGTTGTAATCTGAAAAAGAAATATGAACCCCTTAACTATTTAAAAGTACTAGACAACCAAGTTTTAATTGAGTTTTTCCAGGTTAATTTTTACAAAATATTTTTGAATATAAAAAGATTCTCGATCATAACACGGTGTTATGTGAATCAGCCACGAATTTTTTTGAGGAGTTCCTTAACAATTTTGTTGAGCCATTTAAAGTATCTCTTCCAATCGAATCATCCATGCGAATGCAACATTTTAGTGAGGGAGTCTTGTACTCACAAAAGAATGGCATATTATTCTATTCAGTGTCTAGCCGGTTTCTTCTGTTTGATAAGCTTTCTTCATATCCTACTCTTCCAAGCCTTGGTTTGGGGATCTCATTTTCCAACACAAGGGTTTGTTTCCCTCCCTTTTAACCGATCATACTACCACATTCAGAGGCCTTACGATGTTCCCGAAAATCAGCGCTACAGCTTCATCAATGGAGTTCATAAATGTTGGGTCTACTCCACGGACAAACCTCACACTACTACCAGCCAAACCCTGCCTCGTACTGAGATCGCCATACAAGGATACGGTTACTCTTCTGGTGTGTGGGAATTTGAAGCATATGGGTATGTTCCTTATGGGACATCAGGTGTGTGCATAATGCAAGTTTTCGGAGCAACTGCTCCTCATGCTACAACCCTAATGCTTAGGGTTTATAATGGCTCACTCACATACTACCGGGAAACGGTTCTGGTACCCGACATCTACGATAAATGGTTCAAGCTCAATGTGATTCACGATGTCGATGCTGCGAAAGTGAAGGTCTACATCGATGGACGCCTCAAATTAGAGGCAGATGGTCGCGGAGGGACGTCTCATGCCTTCAAATGTGGTGTATACGCCCAGAATAATGATTCGTGTTATATGGAGTCTCGTTGGAAACACATTAAAGTTTTAACGAAGAGCGGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

1365

Amino Acids

156.3

Weight (kDa)

9.68

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3837
AatII GACGTC 1 cut(s) 3995
Acc65I GGTACC 1 cut(s) 3874
AccB1I GGYRCC 1 cut(s) 3874
AccB7I CCANNNNNTGG 1 cut(s) 3496
AccBSI CCGCTC 1 cut(s) 4086
AccI GTMKAC 4 cut(s) 1513, 3636, 3945, 4017
AccII CGCG 4 cut(s) 1656, 1740, 1831, 3984
AclWI GGATC 6 cut(s) 366, 1440, 1932, 2649, 2914, 3514
AcoI YGGCCR 2 cut(s) 385, 1776
AcsI RAATTY 8 cut(s) 34, 83, 1051, 1937, 1978, 3079, 3303, 3731
AcvI CACGTG 1 cut(s) 1087
AcyI GRCGYC 2 cut(s) 3958, 3992
AdeI CACNNNGTG 1 cut(s) 2327
AfaI GTAC 7 cut(s) 1319, 1953, 2729, 3195, 3406, 3685, 3876
AfeI AGCGCT 1 cut(s) 3599
AflII CTTAAG 1 cut(s) 3086
AflIII ACRYGT 3 cut(s) 176, 1086, 2532
AjnI CCWGG 4 cut(s) 1229, 2054, 2315, 3225
AleI CACNNNNGTG 3 cut(s) 1713, 1741, 1856
AloI GAACNNNNNNTCC 2 cut(s) 1401, 1433
Alw21I GWGCWC 1 cut(s) 1748
AlwI GGATC 6 cut(s) 366, 1440, 1932, 2649, 2914, 3514
Aor51HI AGCGCT 1 cut(s) 3599
ApeKI GCWGC 7 cut(s) 209, 212, 1075, 1111, 1357, 1684, 3929
ApoI RAATTY 8 cut(s) 34, 83, 1051, 1937, 1978, 3079, 3303, 3731
ArsI GACNNNNNNTTYG 4 cut(s) 1401, 1433, 2235, 2267
AseI ATTAAT 2 cut(s) 411, 2217
Asp700I GAANNNNTTC 2 cut(s) 734, 3867
Asp718I GGTACC 1 cut(s) 3874
AspLEI GCGC 1 cut(s) 3600
AspS9I GGNCC 6 cut(s) 310, 1264, 1616, 1705, 1800, 1821
AsuC2I CCSGG 4 cut(s) 1366, 1520, 1580, 3860
AsuHPI GGTGA 7 cut(s) 518, 1445, 1579, 1818, 1822, 2146, 2677
AsuII TTCGAA 1 cut(s) 2203
AvaII GGWCC 3 cut(s) 310, 1800, 1821
BaeGI GKGCMC 1 cut(s) 1578
BanI GGYRCC 1 cut(s) 3874
BanII GRGCYC 1 cut(s) 1759
BauI CACGAG 2 cut(s) 1878, 1919
BbrPI CACGTG 1 cut(s) 1087
BbsI GAAGAC 1 cut(s) 759
Bbv12I GWGCWC 1 cut(s) 1748
BbvI GCAGC 7 cut(s) 196, 224, 1062, 1098, 1344, 1671, 3916
BccI CCATC 8 cut(s) 343, 1179, 1581, 1594, 1781, 2059, 3947, 3971
BceAI ACGGC 1 cut(s) 2292
BcgI CGANNNNNNTGC 2 cut(s) 3908, 3942
BciT130I CCWGG 4 cut(s) 1231, 2056, 2317, 3227
BciVI GTATCC 1 cut(s) 3698
BcnI CCSGG 4 cut(s) 1366, 1520, 1580, 3860
BfmI CTRYAG 5 cut(s) 210, 2469, 2576, 2612, 3601
BfoI RGCGCY 1 cut(s) 3601
BfrI CTTAAG 1 cut(s) 3086
BfuI GTATCC 1 cut(s) 3698
BmcAI AGTACT 1 cut(s) 3195
Bme1390I CCNGG 8 cut(s) 1231, 1366, 1520, 1580, 2056, 2317, 3227, 3860
Bme18I GGWCC 3 cut(s) 310, 1800, 1821
BmgT120I GGNCC 6 cut(s) 310, 1264, 1616, 1705, 1800, 1821
BmiI GGNNCC 7 cut(s) 311, 616, 1032, 1266, 1863, 3061, 3876
BmrFI CCNGG 8 cut(s) 1231, 1366, 1520, 1580, 2056, 2317, 3227, 3860
BmsI GCATC 6 cut(s) 38, 1182, 1723, 2142, 2975, 3916
BpiI GAAGAC 1 cut(s) 759
BplI GAGNNNNNCTC 2 cut(s) 2995, 3027
Bpu10I CCTNAGC 1 cut(s) 3827
Bpu14I TTCGAA 1 cut(s) 2203
BpuMI CCSGG 4 cut(s) 1366, 1520, 1580, 3860
Bsa29I ATCGAT 3 cut(s) 370, 2271, 3951
BsaAI YACGTR 1 cut(s) 1087
BsaHI GRCGYC 2 cut(s) 3958, 3992
BsaI GGTCTC 4 cut(s) 543, 640, 2631, 2780
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 2449, 2479
Bse118I RCCGGY 2 cut(s) 2336, 3446
Bse1I ACTGG 1 cut(s) 97
Bse3DI GCAATG 1 cut(s) 2232
BseBI CCWGG 4 cut(s) 1231, 2056, 2317, 3227
BseCI ATCGAT 3 cut(s) 370, 2271, 3951
BseGI GGATG 9 cut(s) 235, 245, 333, 1197, 1605, 1792, 2051, 2254, 3367
BseMI GCAATG 1 cut(s) 2232
BseMII CTCAG 3 cut(s) 893, 2199, 3678
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 2 cut(s) 3328, 3795
BseSI GKGCMC 1 cut(s) 1578
BseXI GCAGC 7 cut(s) 196, 224, 1062, 1098, 1344, 1671, 3916
BseYI CCCAGC 3 cut(s) 885, 1613, 1702
BsgI GTGCAG 1 cut(s) 536
Bsh1236I CGCG 4 cut(s) 1656, 1740, 1831, 3984
BshNI GGYRCC 1 cut(s) 3874
BshVI ATCGAT 3 cut(s) 370, 2271, 3951
BsiHKAI GWGCWC 1 cut(s) 1748
BsiSI CCGG 7 cut(s) 313, 1365, 1519, 1580, 2337, 3447, 3859
BslFI GGGAC 4 cut(s) 1381, 2963, 3775, 4003
BsmBI CGTCTC 3 cut(s) 2135, 2540, 3999
BsmFI GGGAC 4 cut(s) 1381, 2963, 3775, 4003
BsmI GAATGC 5 cut(s) 68, 173, 642, 2092, 3383
Bso31I GGTCTC 4 cut(s) 543, 640, 2631, 2780
Bsp119I TTCGAA 1 cut(s) 2203
Bsp1286I GDGCHC 3 cut(s) 1578, 1748, 1759
Bsp19I CCATGG 1 cut(s) 1842
BspCNI CTCAG 3 cut(s) 894, 2198, 3679
BspDI ATCGAT 3 cut(s) 370, 2271, 3951
BspFNI CGCG 4 cut(s) 1656, 1740, 1831, 3984
BspLI GGNNCC 7 cut(s) 311, 616, 1032, 1266, 1863, 3061, 3876
BspMAI CTGCAG 2 cut(s) 214, 2616
BspPI GGATC 6 cut(s) 366, 1440, 1932, 2649, 2914, 3514
BspQI GCTCTTC 2 cut(s) 1085, 4076
BspT104I TTCGAA 1 cut(s) 2203
BspT107I GGYRCC 1 cut(s) 3874
BspTI CTTAAG 1 cut(s) 3086
BspTNI GGTCTC 4 cut(s) 543, 640, 2631, 2780
BsrBI CCGCTC 1 cut(s) 4086
BsrDI GCAATG 1 cut(s) 2232
BsrFI RCCGGY 2 cut(s) 2336, 3446
BsrI ACTGG 1 cut(s) 97
BssAI RCCGGY 2 cut(s) 2336, 3446
BssNAI GTATAC 1 cut(s) 4018
BssNI GRCGYC 2 cut(s) 3958, 3992
BssSI CACGAG 2 cut(s) 1878, 1919
BssT1I CCWWGG 5 cut(s) 388, 665, 1782, 1842, 3495
Bst1107I GTATAC 1 cut(s) 4018
Bst2BI CACGAG 2 cut(s) 1878, 1919
Bst2UI CCWGG 4 cut(s) 1231, 2056, 2317, 3227
Bst4CI ACNGT 9 cut(s) 1273, 1804, 2526, 2580, 2683, 2782, 3282, 3710, 3868
Bst6I CTCTTC 6 cut(s) 1085, 2942, 3359, 3491, 3721, 4076
BstACI GRCGYC 2 cut(s) 3958, 3992
BstAFI CTTAAG 1 cut(s) 3086
BstAPI GCANNNNNTGC 1 cut(s) 2152
BstBAI YACGTR 1 cut(s) 1087
BstBI TTCGAA 1 cut(s) 2203
BstC8I GCNNGC 4 cut(s) 1139, 1700, 1738, 4088
BstDEI CTNAG 8 cut(s) 456, 902, 1133, 2185, 2420, 2960, 3687, 3827
BstDSI CCRYGG 7 cut(s) 1290, 1619, 1803, 1829, 1842, 1866, 3642
BstENI CCTNNNNNAGG 2 cut(s) 1062, 1404
BstF5I GGATG 9 cut(s) 235, 245, 333, 1197, 1605, 1792, 2051, 2254, 3367
BstFNI CGCG 4 cut(s) 1656, 1740, 1831, 3984
BstH2I RGCGCY 1 cut(s) 3601
BstHHI GCGC 1 cut(s) 3600
BstMWI GCNNNNNNNGC 6 cut(s) 1111, 1704, 1778, 2098, 2152, 3809
BstNI CCWGG 4 cut(s) 1231, 2056, 2317, 3227
BstNSI RCATGY 2 cut(s) 1429, 2536
BstSCI CCNGG 8 cut(s) 1229, 1364, 1518, 1578, 2054, 2315, 3225, 3858
BstSFI CTRYAG 5 cut(s) 210, 2469, 2576, 2612, 3601
BstSLI GKGCMC 1 cut(s) 1578
BstUI CGCG 4 cut(s) 1656, 1740, 1831, 3984
BstV1I GCAGC 7 cut(s) 196, 224, 1062, 1098, 1344, 1671, 3916
BstV2I GAAGAC 1 cut(s) 759
BstX2I RGATCY 4 cut(s) 1432, 2654, 2919, 3506
BstXI CCANNNNNNTGG 3 cut(s) 1679, 1715, 1858
BstYI RGATCY 4 cut(s) 1432, 2654, 2919, 3506
BstZ17I GTATAC 1 cut(s) 4018
Bsu15I ATCGAT 3 cut(s) 370, 2271, 3951
BsuI GTATCC 1 cut(s) 3698
BsuTUI ATCGAT 3 cut(s) 370, 2271, 3951
BtgI CCRYGG 7 cut(s) 1290, 1619, 1803, 1829, 1842, 1866, 3642
BtsCI GGATG 9 cut(s) 235, 245, 333, 1197, 1605, 1792, 2051, 2254, 3367
BtsI GCAGTG 3 cut(s) 16, 1505, 1851
BtsIMutI CAGTG 6 cut(s) 16, 1505, 1851, 2585, 2778, 3443
Cac8I GCNNGC 4 cut(s) 1139, 1700, 1738, 4088
CfoI GCGC 1 cut(s) 3600
Cfr10I RCCGGY 2 cut(s) 2336, 3446
Cfr13I GGNCC 6 cut(s) 310, 1264, 1616, 1705, 1800, 1821
Cfr42I CCGCGG 1 cut(s) 1832
ClaI ATCGAT 3 cut(s) 370, 2271, 3951
CpoI CGGWCCG 1 cut(s) 1800
CseI GACGC 2 cut(s) 2152, 3966
Csp6I GTAC 7 cut(s) 1318, 1952, 2728, 3194, 3405, 3684, 3875
CspCI CAANNNNNGTGG 2 cut(s) 935, 970
CspI CGGWCCG 1 cut(s) 1800
CviQI GTAC 7 cut(s) 1318, 1952, 2728, 3194, 3405, 3684, 3875
DdeI CTNAG 8 cut(s) 456, 902, 1133, 2185, 2420, 2960, 3687, 3827
DraI TTTAAA 4 cut(s) 345, 3034, 3189, 3345
DraIII CACNNNGTG 1 cut(s) 2327
EaeI YGGCCR 2 cut(s) 385, 1776
Eam1104I CTCTTC 6 cut(s) 1085, 2942, 3359, 3491, 3721, 4076
EarI CTCTTC 6 cut(s) 1085, 2942, 3359, 3491, 3721, 4076
EciI GGCGGA 2 cut(s) 2116, 2348
Eco130I CCWWGG 5 cut(s) 388, 665, 1782, 1842, 3495
Eco147I AGGCCT 2 cut(s) 811, 3574
Eco24I GRGCYC 1 cut(s) 1759
Eco31I GGTCTC 4 cut(s) 543, 640, 2631, 2780
Eco47I GGWCC 3 cut(s) 310, 1800, 1821
Eco47III AGCGCT 1 cut(s) 3599
Eco72I CACGTG 1 cut(s) 1087
EcoNI CCTNNNNNAGG 2 cut(s) 1062, 1404
EcoRII CCWGG 4 cut(s) 1229, 2054, 2315, 3225
EcoT14I CCWWGG 5 cut(s) 388, 665, 1782, 1842, 3495
EcoT22I ATGCAT 1 cut(s) 642
EcoT38I GRGCYC 1 cut(s) 1759
ErhI CCWWGG 5 cut(s) 388, 665, 1782, 1842, 3495
Esp3I CGTCTC 3 cut(s) 2135, 2540, 3999
FalI AAGNNNNNCTT 4 cut(s) 2807, 2839, 3338, 3370
FaqI GGGAC 4 cut(s) 1381, 2963, 3775, 4003
FauI CCCGC 4 cut(s) 1733, 1824, 2940, 4079
FauNDI CATATG 2 cut(s) 3145, 3742
FblI GTMKAC 4 cut(s) 1513, 3636, 3945, 4017
FokI GGATG 9 cut(s) 242, 252, 340, 1204, 1612, 1799, 2038, 2261, 3354
FriOI GRGCYC 1 cut(s) 1759
GlaI GCGC 1 cut(s) 3599
GsaI CCCAGC 3 cut(s) 889, 1617, 1706
HaeII RGCGCY 1 cut(s) 3601
HapII CCGG 7 cut(s) 313, 1365, 1519, 1580, 2337, 3447, 3859
HgaI GACGC 2 cut(s) 2152, 3966
HhaI GCGC 1 cut(s) 3600
Hin1I GRCGYC 2 cut(s) 3958, 3992
Hin6I GCGC 1 cut(s) 3598
HinP1I GCGC 1 cut(s) 3598
HincII GTYRAC 1 cut(s) 2671
HindII GTYRAC 1 cut(s) 2671
HindIII AAGCTT 2 cut(s) 2105, 3466
HpaI GTTAAC 1 cut(s) 2671
HpaII CCGG 7 cut(s) 313, 1365, 1519, 1580, 2337, 3447, 3859
HphI GGTGA 7 cut(s) 518, 1445, 1579, 1818, 1822, 2146, 2677
Hpy99I CGWCG 1 cut(s) 1694
HpyCH4III ACNGT 9 cut(s) 1273, 1804, 2526, 2580, 2683, 2782, 3282, 3710, 3868
HpyCH4IV ACGT 9 cut(s) 178, 1086, 1370, 1965, 2124, 2539, 2549, 2844, 3992
HpyF10VI GCNNNNNNNGC 6 cut(s) 1111, 1704, 1778, 2098, 2152, 3809
HpyF3I CTNAG 8 cut(s) 456, 902, 1133, 2185, 2420, 2960, 3687, 3827
HpySE526I ACGT 9 cut(s) 178, 1086, 1370, 1965, 2124, 2539, 2549, 2844, 3992
Hsp92I GRCGYC 2 cut(s) 3958, 3992
HspAI GCGC 1 cut(s) 3598
KpnI GGTACC 1 cut(s) 3878
KspAI GTTAAC 1 cut(s) 2671
KspI CCGCGG 1 cut(s) 1832
LguI GCTCTTC 2 cut(s) 1085, 4076
LmnI GCTCC 8 cut(s) 191, 874, 1030, 1200, 1522, 1695, 3794, 3808
Lsp1109I GCAGC 7 cut(s) 196, 224, 1062, 1098, 1344, 1671, 3916
LweI GCATC 6 cut(s) 38, 1182, 1723, 2142, 2975, 3916
MaeII ACGT 9 cut(s) 178, 1086, 1370, 1965, 2124, 2539, 2549, 2844, 3992
MbiI CCGCTC 1 cut(s) 4086
MflI RGATCY 4 cut(s) 1432, 2654, 2919, 3506
MhlI GDGCHC 3 cut(s) 1578, 1748, 1759
MlyI GAGTC 3 cut(s) 1509, 3407, 4058
MmeI TCCRAC 4 cut(s) 899, 1410, 3542, 4037
Mph1103I ATGCAT 1 cut(s) 642
MroXI GAANNNNTTC 2 cut(s) 734, 3867
MslI CAYNNNNRTG 6 cut(s) 1677, 1713, 1741, 1856, 3376, 3389
MspA1I CMGCKG 2 cut(s) 1613, 1831
MspCI CTTAAG 1 cut(s) 3086
MspI CCGG 7 cut(s) 313, 1365, 1519, 1580, 2337, 3447, 3859
MspR9I CCNGG 8 cut(s) 1231, 1366, 1520, 1580, 2056, 2317, 3227, 3860
Mva1269I GAATGC 5 cut(s) 68, 173, 642, 2092, 3383
MvaI CCWGG 4 cut(s) 1231, 2056, 2317, 3227
MvnI CGCG 4 cut(s) 1656, 1740, 1831, 3984
MwoI GCNNNNNNNGC 6 cut(s) 1111, 1704, 1778, 2098, 2152, 3809
NciI CCSGG 4 cut(s) 1366, 1520, 1580, 3860
NcoI CCATGG 1 cut(s) 1842
NdeI CATATG 2 cut(s) 3145, 3742
NlaIV GGNNCC 7 cut(s) 311, 616, 1032, 1266, 1863, 3061, 3876
NmeAIII GCCGAG 2 cut(s) 1727, 1738
NmuCI GTSAC 5 cut(s) 361, 1810, 1906, 2120, 2683
NsiI ATGCAT 1 cut(s) 642
NspI RCATGY 2 cut(s) 1429, 2536
NspV TTCGAA 1 cut(s) 2203
OliI CACNNNNGTG 3 cut(s) 1713, 1741, 1856
PacI TTAATTAA 1 cut(s) 1989
PceI AGGCCT 2 cut(s) 811, 3574
PciI ACATGT 1 cut(s) 2532
PciSI GCTCTTC 2 cut(s) 1085, 4076
PcsI WCGNNNNNNNCGW 1 cut(s) 3585
PctI GAATGC 5 cut(s) 68, 173, 642, 2092, 3383
PdmI GAANNNNTTC 2 cut(s) 734, 3867
PflFI GACNNNGTC 1 cut(s) 457
PflMI CCANNNNNTGG 1 cut(s) 3496
PfoI TCCNGGA 2 cut(s) 1518, 2315
PleI GAGTC 3 cut(s) 1509, 3406, 4057
PmaCI CACGTG 1 cut(s) 1087
PmlI CACGTG 1 cut(s) 1087
PpsI GAGTC 3 cut(s) 1509, 3406, 4057
Ppu21I YACGTR 1 cut(s) 1087
PscI ACATGT 1 cut(s) 2532
PshBI ATTAAT 2 cut(s) 411, 2217
PsiI TTATAA 1 cut(s) 3837
Psp6I CCWGG 4 cut(s) 1229, 2054, 2315, 3225
PspCI CACGTG 1 cut(s) 1087
PspFI CCCAGC 3 cut(s) 885, 1613, 1702
PspGI CCWGG 4 cut(s) 1229, 2054, 2315, 3225
PspN4I GGNNCC 7 cut(s) 311, 616, 1032, 1266, 1863, 3061, 3876
PspPI GGNCC 6 cut(s) 310, 1264, 1616, 1705, 1800, 1821
PstI CTGCAG 2 cut(s) 214, 2616
PsuI RGATCY 4 cut(s) 1432, 2654, 2919, 3506
PsyI GACNNNGTC 1 cut(s) 457
PvuII CAGCTG 1 cut(s) 1613
RsaI GTAC 7 cut(s) 1319, 1953, 2729, 3195, 3406, 3685, 3876
RsaNI GTAC 7 cut(s) 1318, 1952, 2728, 3194, 3405, 3684, 3875
RseI CAYNNNNRTG 6 cut(s) 1677, 1713, 1741, 1856, 3376, 3389
Rsr2I CGGWCCG 1 cut(s) 1800
RsrII CGGWCCG 1 cut(s) 1800
SacII CCGCGG 1 cut(s) 1832
SapI GCTCTTC 2 cut(s) 1085, 4076
Sau96I GGNCC 6 cut(s) 310, 1264, 1616, 1705, 1800, 1821
ScaI AGTACT 1 cut(s) 3195
SchI GAGTC 3 cut(s) 1509, 3407, 4058
ScrFI CCNGG 8 cut(s) 1231, 1366, 1520, 1580, 2056, 2317, 3227, 3860
SduI GDGCHC 3 cut(s) 1578, 1748, 1759
SfaNI GCATC 6 cut(s) 38, 1182, 1723, 2142, 2975, 3916
SfcI CTRYAG 5 cut(s) 210, 2469, 2576, 2612, 3601
Sfr303I CCGCGG 1 cut(s) 1832
SfuI TTCGAA 1 cut(s) 2203
SgrBI CCGCGG 1 cut(s) 1832
SinI GGWCC 3 cut(s) 310, 1800, 1821
SmiMI CAYNNNNRTG 6 cut(s) 1677, 1713, 1741, 1856, 3376, 3389
SmlI CTYRAG 1 cut(s) 3086
SmoI CTYRAG 1 cut(s) 3086
SseBI AGGCCT 2 cut(s) 811, 3574
SspI AATATT 3 cut(s) 404, 2664, 3246
StuI AGGCCT 2 cut(s) 811, 3574
StyD4I CCNGG 8 cut(s) 1229, 1364, 1518, 1578, 2054, 2315, 3225, 3858
StyI CCWWGG 5 cut(s) 388, 665, 1782, 1842, 3495
TaaI ACNGT 9 cut(s) 1273, 1804, 2526, 2580, 2683, 2782, 3282, 3710, 3868
TaiI ACGT 9 cut(s) 181, 1089, 1373, 1968, 2127, 2542, 2552, 2847, 3995
TaqII GACCGA 1 cut(s) 1013
TatI WGTACW 5 cut(s) 1317, 1951, 2727, 3193, 3404
TauI GCSGC 7 cut(s) 387, 501, 552, 593, 1638, 1656, 1781
TscAI CASTG 6 cut(s) 23, 1505, 1858, 2585, 2785, 3443
TseFI GTSAC 5 cut(s) 361, 1810, 1906, 2120, 2683
TseI GCWGC 7 cut(s) 209, 212, 1075, 1111, 1357, 1684, 3929
Tsp45I GTSAC 5 cut(s) 361, 1810, 1906, 2120, 2683
TspGWI ACGGA 2 cut(s) 1279, 3659
TspRI CASTG 6 cut(s) 23, 1505, 1858, 2585, 2785, 3443
Tth111I GACNNNGTC 1 cut(s) 457
Van91I CCANNNNNTGG 1 cut(s) 3496
Vha464I CTTAAG 1 cut(s) 3086
VpaK11BI GGWCC 3 cut(s) 310, 1800, 1821
VspI ATTAAT 2 cut(s) 411, 2217
XagI CCTNNNNNAGG 2 cut(s) 1062, 1404
XapI RAATTY 8 cut(s) 34, 83, 1051, 1937, 1978, 3079, 3303, 3731
XceI RCATGY 2 cut(s) 1429, 2536
XmiI GTMKAC 4 cut(s) 1513, 3636, 3945, 4017
XmnI GAANNNNTTC 2 cut(s) 734, 3867
ZraI GACGTC 1 cut(s) 3993
ZrmI AGTACT 1 cut(s) 3195
Zsp2I ATGCAT 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.