MD10G1027900.v1.1

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000532
Physical Location & Seq
Reverse (-)
3607227 .. 3608009
783 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1027900.v1.1.491

Sequence Viewer

Length: 666 bp
ATGTTGTTTATAGACTGCAGGAAAATGAATTCCTTCTACAAGTTCTTTTGCTTGTTCTTGTTGATCCTGAAATACCACCATCTTTGTGCCGCTGATCCTACTGATGGATTCACCCCTGTACCATTGACAGAAGACAACTTTGTGTTACAAAAACCATATAATGAACCCCTCGATGATCGTTACAGTTACAAGGATGGAGTTCGAAGTTTTTGGATATACAACCATGACAAGCCCTTCAAAGCGGACAGCACAACTAGGCCGCGGTCAGAGCTGCGCATTTCGGGGCATGACTATTCTTCTGGAATTTGGCAATTTGAAGGCTATACATATGTGCCAAGTGGTACTTCTGGGGTTACAATAGTGCAGATCCACGGTGCAGCTGAGGGAGCGACATCTCTACAGCTAAGGATGTATGACGGAGATATCAAATACTACAAATACAACGTCGTAGCTACTGATCTTTACGACAAGTGGTTCCGCGTAAACATAATCCACAACGTTGACAAAGGGAAGATTATAGTTTTCATTGATGCTGTCAAGAAGTTTGTGGTGAAAGATCAAGGACCAGGGGATCTGTATTTCAAATGTGGAGTATATGCTGCACCAGAGAATTCGAGTAACTTCATGGAATCAAGGTGGAAAGAGATCAAACTTTACAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

222

Amino Acids

25.73

Weight (kDa)

8.49

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 45 - 219 4.6e-26 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 275
AccII CGCG 2 cut(s) 262, 480
AciI CCGC 5 cut(s) 90, 242, 260, 262, 478
AclI AACGTT 1 cut(s) 498
AclWI GGATC 4 cut(s) 58, 89, 361, 579
AcsI RAATTY 3 cut(s) 28, 303, 610
AfaI GTAC 2 cut(s) 120, 343
AfiI CCNNNNNNNGG 1 cut(s) 104
AgsI TTSAA 3 cut(s) 238, 317, 583
AjnI CCWGG 1 cut(s) 565
AjuI GAANNNNNNNTTGG 2 cut(s) 328, 360
AluBI AGCT 4 cut(s) 271, 380, 403, 452
AluI AGCT 4 cut(s) 271, 380, 403, 452
AlwI GGATC 4 cut(s) 58, 89, 361, 579
AoxI GGCC 1 cut(s) 257
ApeKI GCWGC 3 cut(s) 271, 377, 599
ApoI RAATTY 3 cut(s) 28, 303, 610
ArsI GACNNNNNNTTYG 2 cut(s) 429, 461
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 563
AsuHPI GGTGA 2 cut(s) 103, 562
AsuII TTCGAA 1 cut(s) 202
AvaII GGWCC 1 cut(s) 563
BaeI ACNNNNGTAYC 2 cut(s) 102, 135
BbsI GAAGAC 1 cut(s) 138
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 3 cut(s) 258, 389, 586
BccI CCATC 3 cut(s) 87, 98, 188
BciT130I CCWGG 1 cut(s) 567
BfaI CTAG 1 cut(s) 255
BfmI CTRYAG 2 cut(s) 16, 398
BisI GCNGC 5 cut(s) 90, 260, 272, 378, 600
BlsI GCNGC 5 cut(s) 91, 261, 273, 379, 601
Bme1390I CCNGG 1 cut(s) 567
Bme18I GGWCC 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 563
BmiI GGNNCC 1 cut(s) 476
BmrFI CCNGG 1 cut(s) 567
BmsI GCATC 1 cut(s) 520
BpiI GAAGAC 1 cut(s) 138
Bpu10I CCTNAGC 2 cut(s) 381, 404
Bpu14I TTCGAA 1 cut(s) 202
BsaJI CCNNGG 3 cut(s) 260, 370, 566
Bsc4I CCNNNNNNNGG 1 cut(s) 104
BseBI CCWGG 1 cut(s) 567
BseDI CCNNGG 3 cut(s) 260, 370, 566
BseGI GGATG 2 cut(s) 199, 414
BseLI CCNNNNNNNGG 1 cut(s) 104
BseMII CTCAG 1 cut(s) 372
BseXI GCAGC 3 cut(s) 258, 389, 586
BsgI GTGCAG 3 cut(s) 383, 396, 585
Bsh1236I CGCG 2 cut(s) 262, 480
BshFI GGCC 1 cut(s) 259
BslI CCNNNNNNNGG 1 cut(s) 104
BsnI GGCC 1 cut(s) 259
Bsp119I TTCGAA 1 cut(s) 202
Bsp143I GATC 8 cut(s) 63, 94, 175, 366, 457, 556, 571, 645
BspACI CCGC 5 cut(s) 90, 242, 260, 262, 478
BspANI GGCC 1 cut(s) 259
BspCNI CTCAG 1 cut(s) 373
BspFNI CGCG 2 cut(s) 262, 480
BspLI GGNNCC 1 cut(s) 476
BspMAI CTGCAG 1 cut(s) 20
BspPI GGATC 4 cut(s) 58, 89, 361, 579
BspT104I TTCGAA 1 cut(s) 202
BssECI CCNNGG 3 cut(s) 260, 370, 566
BssMI GATC 8 cut(s) 63, 94, 175, 366, 457, 556, 571, 645
Bst2UI CCWGG 1 cut(s) 567
Bst4CI ACNGT 2 cut(s) 185, 374
BstBI TTCGAA 1 cut(s) 202
BstDEI CTNAG 2 cut(s) 381, 404
BstDSI CCRYGG 2 cut(s) 260, 370
BstF5I GGATG 2 cut(s) 199, 414
BstFNI CGCG 2 cut(s) 262, 480
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 8 cut(s) 66, 97, 178, 369, 460, 559, 574, 648
BstMBI GATC 8 cut(s) 63, 94, 175, 366, 457, 556, 571, 645
BstMWI GCNNNNNNNGC 2 cut(s) 268, 386
BstNI CCWGG 1 cut(s) 567
BstSCI CCNGG 1 cut(s) 565
BstSFI CTRYAG 2 cut(s) 16, 398
BstUI CGCG 2 cut(s) 262, 480
BstV1I GCAGC 3 cut(s) 258, 389, 586
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 2 cut(s) 366, 571
BstYI RGATCY 2 cut(s) 366, 571
BsuRI GGCC 1 cut(s) 259
BtgI CCRYGG 2 cut(s) 260, 370
BtsCI GGATG 2 cut(s) 199, 414
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 1 cut(s) 563
Cfr42I CCGCGG 1 cut(s) 263
Csp6I GTAC 2 cut(s) 119, 342
CspCI CAANNNNNGTGG 2 cut(s) 65, 100
CviAII CATG 3 cut(s) 224, 287, 625
CviJI RGCY 7 cut(s) 232, 259, 271, 321, 380, 403, 452
CviKI_1 RGCY 7 cut(s) 232, 259, 271, 321, 380, 403, 452
CviQI GTAC 2 cut(s) 119, 342
DdeI CTNAG 2 cut(s) 381, 404
DpnI GATC 8 cut(s) 65, 96, 177, 368, 459, 558, 573, 647
DpnII GATC 8 cut(s) 63, 94, 175, 366, 457, 556, 571, 645
Eco32I GATATC 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 563
EcoRI GAATTC 2 cut(s) 28, 610
EcoRII CCWGG 1 cut(s) 565
EcoRV GATATC 1 cut(s) 424
FaeI CATG 3 cut(s) 227, 290, 628
FalI AAGNNNNNCTT 2 cut(s) 328, 360
FatI CATG 3 cut(s) 223, 286, 624
FauNDI CATATG 1 cut(s) 328
Fnu4HI GCNGC 5 cut(s) 90, 260, 272, 378, 600
FokI GGATG 2 cut(s) 206, 421
Fsp4HI GCNGC 5 cut(s) 90, 260, 272, 378, 600
FspBI CTAG 1 cut(s) 255
FspI TGCGCA 1 cut(s) 275
GlaI GCGC 1 cut(s) 275
GluI GCNGC 5 cut(s) 90, 260, 272, 378, 600
HaeIII GGCC 1 cut(s) 259
HhaI GCGC 1 cut(s) 276
Hin1II CATG 3 cut(s) 227, 290, 628
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HinfI GANTC 2 cut(s) 108, 629
HphI GGTGA 2 cut(s) 103, 562
Hpy166II GTNNAC 2 cut(s) 484, 502
Hpy188I TCNGA 1 cut(s) 268
Hpy188III TCNNGA 3 cut(s) 67, 300, 538
Hpy8I GTNNAC 2 cut(s) 484, 502
Hpy99I CGWCG 1 cut(s) 449
HpyAV CCTTC 3 cut(s) 43, 244, 311
HpyCH4III ACNGT 2 cut(s) 185, 374
HpyCH4IV ACGT 2 cut(s) 444, 498
HpyCH4V TGCA 4 cut(s) 18, 364, 377, 602
HpyF10VI GCNNNNNNNGC 2 cut(s) 268, 386
HpyF3I CTNAG 2 cut(s) 381, 404
HpySE526I ACGT 2 cut(s) 444, 498
Hsp92II CATG 3 cut(s) 227, 290, 628
HspAI GCGC 1 cut(s) 274
KspI CCGCGG 1 cut(s) 263
Kzo9I GATC 8 cut(s) 63, 94, 175, 366, 457, 556, 571, 645
LmnI GCTCC 1 cut(s) 386
LpnPI CCDG 8 cut(s) 4, 80, 129, 285, 333, 552, 579, 618
Lsp1109I GCAGC 3 cut(s) 258, 389, 586
LweI GCATC 1 cut(s) 520
MaeI CTAG 1 cut(s) 255
MaeII ACGT 2 cut(s) 444, 498
MaeIII GTNAC 5 cut(s) 144, 179, 185, 352, 617
MalI GATC 8 cut(s) 65, 96, 177, 368, 459, 558, 573, 647
MboI GATC 8 cut(s) 63, 94, 175, 366, 457, 556, 571, 645
MboII GAAGA 3 cut(s) 143, 288, 523
MflI RGATCY 2 cut(s) 366, 571
MluCI AATT 4 cut(s) 28, 303, 311, 610
MnlI CCTC 2 cut(s) 179, 376
MroXI GAANNNNTTC 1 cut(s) 32
MslI CAYNNNNRTG 1 cut(s) 84
MspA1I CMGCKG 3 cut(s) 92, 262, 380
MspR9I CCNGG 1 cut(s) 567
MvaI CCWGG 1 cut(s) 567
MvnI CGCG 2 cut(s) 262, 480
MwoI GCNNNNNNNGC 2 cut(s) 268, 386
NdeI CATATG 1 cut(s) 328
NdeII GATC 8 cut(s) 63, 94, 175, 366, 457, 556, 571, 645
NlaIII CATG 3 cut(s) 227, 290, 628
NlaIV GGNNCC 1 cut(s) 476
NsbI TGCGCA 1 cut(s) 275
NspV TTCGAA 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 2 cut(s) 108, 629
PkrI GCNGC 5 cut(s) 91, 261, 273, 379, 601
Psp1406I AACGTT 1 cut(s) 498
Psp6I CCWGG 1 cut(s) 565
PspGI CCWGG 1 cut(s) 565
PspN4I GGNNCC 1 cut(s) 476
PspPI GGNCC 1 cut(s) 563
PstI CTGCAG 1 cut(s) 20
PsuI RGATCY 2 cut(s) 366, 571
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 2 cut(s) 120, 343
RsaNI GTAC 2 cut(s) 119, 342
RseI CAYNNNNRTG 1 cut(s) 84
SacII CCGCGG 1 cut(s) 263
SatI GCNGC 5 cut(s) 90, 260, 272, 378, 600
Sau3AI GATC 8 cut(s) 63, 94, 175, 366, 457, 556, 571, 645
Sau96I GGNCC 1 cut(s) 563
ScrFI CCNGG 1 cut(s) 567
SetI ASST 7 cut(s) 273, 382, 405, 447, 454, 501, 638
SfaNI GCATC 1 cut(s) 520
SfcI CTRYAG 2 cut(s) 16, 398
Sfr303I CCGCGG 1 cut(s) 263
SfuI TTCGAA 1 cut(s) 202
SgrBI CCGCGG 1 cut(s) 263
SinI GGWCC 1 cut(s) 563
SmiMI CAYNNNNRTG 1 cut(s) 84
Sse9I AATT 4 cut(s) 28, 303, 311, 610
SsiI CCGC 5 cut(s) 90, 242, 260, 262, 478
SspMI CTAG 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 565
TaaI ACNGT 2 cut(s) 185, 374
TaiI ACGT 2 cut(s) 447, 501
TaqI TCGA 3 cut(s) 171, 202, 614
TasI AATT 4 cut(s) 28, 303, 311, 610
TauI GCSGC 2 cut(s) 92, 262
TfiI GAWTC 2 cut(s) 108, 629
TseI GCWGC 3 cut(s) 271, 377, 599
TspDTI ATGAA 4 cut(s) 41, 177, 514, 613
TspGWI ACGGA 1 cut(s) 432
VpaK11BI GGWCC 1 cut(s) 563
XapI RAATTY 3 cut(s) 28, 303, 610
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.