MD10G1028300.v1.1

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000532
Physical Location & Seq
Reverse (-)
3649104 .. 3650635
1532 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1028300.v1.1.491

Sequence Viewer

Length: 657 bp
ATGAGAGGTAGCTACTGCTATATACTTCTGTCCTTGGTTTTTCTGGGCTCACTGAAAGAAATTATTCAGTTATGCGGTGCTGATCCGACTGATGGCTTCACCGATGTTCCGTTAACAGAATATAATTTCGAATTACAGAAACCGTATGACGTACCACTGGAGGAGCGCTACAGTTTTGTCAATGGAGTAAGGCGTTTGTGGGTCTATGCTGATGACAAGCCCCATGACCCTAATAGCCAAACCCAACCACGTACTGAAGTTCGCATACGGGGACTTGACTACTCATCTGGAATATGGCAATTTGAAGGCTATGGATTTGTGCCAAATGGAACCTCTGGTGCTACAGTAGCACAGATCCATGGAGCAGCTAAGGGTGCTACGACTATAATCCTAAGAATCTATAACGGCGACATGAGGTATTATAGTCGAGATTTGGTGGCTACAAATCTTTACGATAAGTGGTTCAGACTTAACATAATCCATGACGTCGACGTAGGGAGCGTGACTGTTTTCATTGACGGAGTCCAGAAATTTCGGGTGAAGGATCAAGGGCCAGGAGATTTGTACTTCAAATGTGGAGTTTATGCTGCACCGGCTAACATAAGCTACTACATGGAATCACGTTGGAAAGACATCAAAATATATAAAAAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

219

Amino Acids

24.97

Weight (kDa)

6.82

Isoelectric Point (pI)

29.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 42 - 215 3.7e-24 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 489
AccI GTMKAC 1 cut(s) 489
AciI CCGC 1 cut(s) 75
AclWI GGATC 3 cut(s) 77, 349, 552
AcsI RAATTY 1 cut(s) 530
AcuI CTGAAG 1 cut(s) 276
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 3 cut(s) 153, 253, 566
AfeI AGCGCT 1 cut(s) 167
AfiI CCNNNNNNNGG 1 cut(s) 92
AgsI TTSAA 2 cut(s) 305, 571
AjnI CCWGG 1 cut(s) 553
AluBI AGCT 3 cut(s) 12, 368, 606
AluI AGCT 3 cut(s) 12, 368, 606
AlwI GGATC 3 cut(s) 77, 349, 552
Aor51HI AGCGCT 1 cut(s) 167
AoxI GGCC 1 cut(s) 551
ApeKI GCWGC 2 cut(s) 365, 587
ApoI RAATTY 1 cut(s) 530
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 1 cut(s) 551
AsuHPI GGTGA 2 cut(s) 91, 550
AsuII TTCGAA 1 cut(s) 129
BanII GRGCYC 1 cut(s) 50
BarI GAAGNNNNNNTAC 2 cut(s) 249, 281
BbvI GCAGC 2 cut(s) 377, 574
BccI CCATC 1 cut(s) 86
BceAI ACGGC 1 cut(s) 421
BciT130I CCWGG 1 cut(s) 555
BfmI CTRYAG 2 cut(s) 169, 342
BfoI RGCGCY 1 cut(s) 169
BisI GCNGC 2 cut(s) 366, 588
BlsI GCNGC 2 cut(s) 367, 589
Bme1390I CCNGG 1 cut(s) 555
BmgT120I GGNCC 1 cut(s) 551
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 1 cut(s) 555
BpmI CTGGAG 1 cut(s) 179
Bpu10I CCTNAGC 1 cut(s) 369
Bpu14I TTCGAA 1 cut(s) 129
BsaAI YACGTR 1 cut(s) 251
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 2 cut(s) 33, 358
Bsc4I CCNNNNNNNGG 1 cut(s) 92
Bse118I RCCGGY 1 cut(s) 592
Bse1I ACTGG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 33, 358
BseLI CCNNNNNNNGG 1 cut(s) 92
BseNI ACTGG 1 cut(s) 162
BseRI GAGGAG 1 cut(s) 176
BseXI GCAGC 2 cut(s) 377, 574
BsgI GTGCAG 1 cut(s) 573
BshFI GGCC 1 cut(s) 553
BsiSI CCGG 1 cut(s) 593
BslFI GGGAC 1 cut(s) 285
BslI CCNNNNNNNGG 1 cut(s) 92
BsmFI GGGAC 1 cut(s) 285
BsnI GGCC 1 cut(s) 553
Bsp119I TTCGAA 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 50
Bsp143I GATC 3 cut(s) 82, 354, 544
Bsp19I CCATGG 1 cut(s) 358
BspACI CCGC 1 cut(s) 75
BspANI GGCC 1 cut(s) 553
BspLI GGNNCC 1 cut(s) 331
BspPI GGATC 3 cut(s) 77, 349, 552
BspT104I TTCGAA 1 cut(s) 129
BsrFI RCCGGY 1 cut(s) 592
BsrI ACTGG 1 cut(s) 162
BssAI RCCGGY 1 cut(s) 592
BssECI CCNNGG 2 cut(s) 33, 358
BssMI GATC 3 cut(s) 82, 354, 544
BssNI GRCGYC 1 cut(s) 486
BssT1I CCWWGG 2 cut(s) 33, 358
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 4 cut(s) 144, 173, 346, 508
BstACI GRCGYC 1 cut(s) 486
BstBAI YACGTR 1 cut(s) 251
BstBI TTCGAA 1 cut(s) 129
BstDEI CTNAG 2 cut(s) 369, 392
BstDSI CCRYGG 1 cut(s) 358
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 3 cut(s) 85, 357, 547
BstMBI GATC 3 cut(s) 82, 354, 544
BstMWI GCNNNNNNNGC 3 cut(s) 347, 374, 593
BstNI CCWGG 1 cut(s) 555
BstSCI CCNGG 1 cut(s) 553
BstSFI CTRYAG 2 cut(s) 169, 342
BstV1I GCAGC 2 cut(s) 377, 574
BstX2I RGATCY 1 cut(s) 354
BstYI RGATCY 1 cut(s) 354
BsuRI GGCC 1 cut(s) 553
BtgI CCRYGG 1 cut(s) 358
BtsIMutI CAGTG 2 cut(s) 50, 155
CfoI GCGC 1 cut(s) 168
Cfr10I RCCGGY 1 cut(s) 592
Cfr13I GGNCC 1 cut(s) 551
Csp6I GTAC 3 cut(s) 152, 252, 565
CviAII CATG 5 cut(s) 224, 359, 412, 482, 613
CviQI GTAC 3 cut(s) 152, 252, 565
DdeI CTNAG 2 cut(s) 369, 392
DpnI GATC 3 cut(s) 84, 356, 546
DpnII GATC 3 cut(s) 82, 354, 544
Eco130I CCWWGG 2 cut(s) 33, 358
Eco24I GRGCYC 1 cut(s) 50
Eco47III AGCGCT 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 276
EcoRII CCWGG 1 cut(s) 553
EcoT14I CCWWGG 2 cut(s) 33, 358
EcoT38I GRGCYC 1 cut(s) 50
ErhI CCWWGG 2 cut(s) 33, 358
FaeI CATG 5 cut(s) 227, 362, 415, 485, 616
FaqI GGGAC 1 cut(s) 285
FatI CATG 5 cut(s) 223, 358, 411, 481, 612
FblI GTMKAC 1 cut(s) 489
Fnu4HI GCNGC 2 cut(s) 366, 588
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 2 cut(s) 366, 588
GlaI GCGC 1 cut(s) 167
GluI GCNGC 2 cut(s) 366, 588
GsuI CTGGAG 1 cut(s) 179
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 1 cut(s) 553
HapII CCGG 1 cut(s) 593
HhaI GCGC 1 cut(s) 168
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 5 cut(s) 227, 362, 415, 485, 616
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HincII GTYRAC 2 cut(s) 114, 490
HindII GTYRAC 2 cut(s) 114, 490
HinfI GANTC 3 cut(s) 396, 522, 617
HpaI GTTAAC 1 cut(s) 114
HpaII CCGG 1 cut(s) 593
HphI GGTGA 2 cut(s) 91, 550
Hpy166II GTNNAC 2 cut(s) 114, 490
Hpy188I TCNGA 2 cut(s) 87, 467
Hpy188III TCNNGA 3 cut(s) 288, 428, 526
Hpy8I GTNNAC 2 cut(s) 114, 490
Hpy99I CGWCG 2 cut(s) 491, 494
HpyAV CCTTC 2 cut(s) 299, 535
HpyCH4III ACNGT 4 cut(s) 144, 173, 346, 508
HpyCH4IV ACGT 5 cut(s) 150, 250, 486, 492, 622
HpyCH4V TGCA 1 cut(s) 590
HpyF10VI GCNNNNNNNGC 3 cut(s) 347, 374, 593
HpyF3I CTNAG 2 cut(s) 369, 392
HpySE526I ACGT 5 cut(s) 150, 250, 486, 492, 622
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 5 cut(s) 227, 362, 415, 485, 616
HspAI GCGC 1 cut(s) 166
KspAI GTTAAC 1 cut(s) 114
Kzo9I GATC 3 cut(s) 82, 354, 544
LmnI GCTCC 3 cut(s) 163, 362, 498
LpnPI CCDG 8 cut(s) 29, 143, 273, 321, 539, 540, 567, 606
Lsp1109I GCAGC 2 cut(s) 377, 574
MaeII ACGT 5 cut(s) 150, 250, 486, 492, 622
MaeIII GTNAC 1 cut(s) 502
MalI GATC 3 cut(s) 84, 356, 546
MboI GATC 3 cut(s) 82, 354, 544
MflI RGATCY 1 cut(s) 354
MhlI GDGCHC 1 cut(s) 50
MluCI AATT 5 cut(s) 60, 124, 131, 299, 530
MlyI GAGTC 1 cut(s) 531
MmeI TCCRAC 2 cut(s) 110, 605
MnlI CCTC 3 cut(s) 154, 343, 408
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 2 cut(s) 113, 471
MspI CCGG 1 cut(s) 593
MspR9I CCNGG 1 cut(s) 555
MvaI CCWGG 1 cut(s) 555
MwoI GCNNNNNNNGC 3 cut(s) 347, 374, 593
NcoI CCATGG 1 cut(s) 358
NdeII GATC 3 cut(s) 82, 354, 544
NlaIII CATG 5 cut(s) 227, 362, 415, 485, 616
NlaIV GGNNCC 1 cut(s) 331
NmuCI GTSAC 1 cut(s) 502
NspV TTCGAA 1 cut(s) 129
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 2 cut(s) 396, 617
PflFI GACNNNGTC 1 cut(s) 521
PkrI GCNGC 2 cut(s) 367, 589
PleI GAGTC 1 cut(s) 530
PpsI GAGTC 1 cut(s) 530
Ppu21I YACGTR 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 553
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 1 cut(s) 331
PspPI GGNCC 1 cut(s) 551
PsuI RGATCY 1 cut(s) 354
PsyI GACNNNGTC 1 cut(s) 521
RsaI GTAC 3 cut(s) 153, 253, 566
RsaNI GTAC 3 cut(s) 152, 252, 565
SalI GTCGAC 1 cut(s) 488
SaqAI TTAA 2 cut(s) 113, 471
SatI GCNGC 2 cut(s) 366, 588
Sau3AI GATC 3 cut(s) 82, 354, 544
Sau96I GGNCC 1 cut(s) 551
SchI GAGTC 1 cut(s) 531
ScrFI CCNGG 1 cut(s) 555
SduI GDGCHC 1 cut(s) 50
SfcI CTRYAG 2 cut(s) 169, 342
SfuI TTCGAA 1 cut(s) 129
SgrDI CGTCGACG 1 cut(s) 488
Sse9I AATT 5 cut(s) 60, 124, 131, 299, 530
SsiI CCGC 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 553
StyI CCWWGG 2 cut(s) 33, 358
TaaI ACNGT 4 cut(s) 144, 173, 346, 508
TaiI ACGT 5 cut(s) 153, 253, 489, 495, 625
TaqI TCGA 3 cut(s) 129, 427, 489
TasI AATT 5 cut(s) 60, 124, 131, 299, 530
TatI WGTACW 1 cut(s) 564
TfiI GAWTC 2 cut(s) 396, 617
Tru1I TTAA 2 cut(s) 113, 471
Tru9I TTAA 2 cut(s) 113, 471
TscAI CASTG 2 cut(s) 57, 162
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 2 cut(s) 365, 587
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 1 cut(s) 502
TspGWI ACGGA 2 cut(s) 99, 534
TspRI CASTG 2 cut(s) 57, 162
Tth111I GACNNNGTC 1 cut(s) 521
XapI RAATTY 1 cut(s) 530
XmiI GTMKAC 1 cut(s) 489
XmnI GAANNNNTTC 1 cut(s) 63
ZraI GACGTC 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.