FvH4_2g13513

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000532
Physical Location & Seq
Forward (+)
11806516 .. 11807592
1077 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13513.t1

Sequence Viewer

Length: 663 bp
ATGATGACGAGCACTAGTACTAGTTTCTGCAAGAATGCCCTGGTTCTGCTACTACTGAGCTCTTTGGGATATTTCTGTAGTACTGCTGCTGATCCTACTGATGGGTTCACCCGTGTGTCGCTGACCAACAACAACTTCAAGTTGCAGAAACCGTACGACAAATCTGTGGCGGAGCGCTACAGCAAAGTCGATGGAGTCGAAACCTTCAAGGTTTACACGAATGACAAGCCCTTTGAACAAGGCAGTCCAACCAGGCCACGCACAGAAATCAGAGTTACAGGATATGACTACACTTCCGGCGTCTGGCAATTCGAGGGCAGTTGCTACGTGCCGTCGCCTACCACCGGCGTTATAATAATGCAGGTCTTCGGGGCGGCCGATGAAGCTACGACTCTGCAACTAAGGAACATCAATGGAGATTTGATGTACTACAACAAGAATGTGGTCGTGAGTAATGTCTACAATAAGTGGGTGAGGGTGAATGTGATTCACAATGTTGGTGCAAAAAAGGTTACCATTTTTGTCGACGGTGTCCAGAAAATGGTGATGGAAGACCACGGTCCAGACACACATTACTTCAAATATGGAGCTTATGCAGCTTTAACTGGTTCAAGTAACTACATGGAGTCAAGGTGGAAGGGAATCAAAATCTTCAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

221

Amino Acids

24.73

Weight (kDa)

9.08

Isoelectric Point (pI)

27.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 44 - 218 8e-25 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 353
Acc36I ACCTGC 1 cut(s) 352
AccB7I CCANNNNNTGG 1 cut(s) 541
AccI GTMKAC 2 cut(s) 459, 525
AciI CCGC 2 cut(s) 170, 374
AclWI GGATC 1 cut(s) 86
AcoI YGGCCR 1 cut(s) 375
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 4 cut(s) 19, 82, 155, 428
AfeI AGCGCT 1 cut(s) 176
AfiI CCNNNNNNNGG 4 cut(s) 101, 303, 344, 541
AgsI TTSAA 6 cut(s) 139, 208, 236, 580, 612, 655
AhlI ACTAGT 2 cut(s) 14, 20
AjnI CCWGG 2 cut(s) 39, 251
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AleI CACNNNNGTG 1 cut(s) 113
AluBI AGCT 4 cut(s) 60, 386, 590, 599
AluI AGCT 4 cut(s) 60, 386, 590, 599
Alw21I GWGCWC 2 cut(s) 14, 62
AlwI GGATC 1 cut(s) 86
Aor51HI AGCGCT 1 cut(s) 176
AoxI GGCC 2 cut(s) 254, 375
ApeKI GCWGC 2 cut(s) 86, 596
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
AspLEI GCGC 1 cut(s) 177
AspS9I GGNCC 1 cut(s) 560
AsuHPI GGTGA 4 cut(s) 100, 484, 490, 556
AvaII GGWCC 1 cut(s) 560
BanII GRGCYC 1 cut(s) 62
BbsI GAAGAC 2 cut(s) 358, 558
Bbv12I GWGCWC 2 cut(s) 14, 62
BbvI GCAGC 2 cut(s) 73, 608
BccI CCATC 3 cut(s) 95, 185, 541
BceAI ACGGC 1 cut(s) 316
BciT130I CCWGG 2 cut(s) 41, 253
BcuI ACTAGT 2 cut(s) 14, 20
BfaI CTAG 2 cut(s) 15, 21
BfmI CTRYAG 2 cut(s) 76, 178
BfoI RGCGCY 1 cut(s) 178
BfuAI ACCTGC 1 cut(s) 352
BisI GCNGC 3 cut(s) 87, 375, 597
BlsI GCNGC 3 cut(s) 88, 376, 598
BmcAI AGTACT 2 cut(s) 19, 82
Bme1390I CCNGG 2 cut(s) 41, 253
Bme18I GGWCC 1 cut(s) 560
BmgT120I GGNCC 1 cut(s) 560
BmrFI CCNGG 2 cut(s) 41, 253
BoxI GACNNNNGTC 1 cut(s) 558
BpiI GAAGAC 2 cut(s) 358, 558
BsaAI YACGTR 1 cut(s) 328
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 39, 556
BsaXI ACNNNNNCTCC 2 cut(s) 617, 647
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 303, 344, 541
Bse118I RCCGGY 1 cut(s) 344
Bse1I ACTGG 1 cut(s) 610
BseBI CCWGG 2 cut(s) 41, 253
BseDI CCNNGG 2 cut(s) 39, 556
BseLI CCNNNNNNNGG 4 cut(s) 101, 303, 344, 541
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 610
BseX3I CGGCCG 1 cut(s) 375
BseXI GCAGC 2 cut(s) 73, 608
Bsh1285I CGRYCG 1 cut(s) 378
BshFI GGCC 2 cut(s) 256, 377
BsiEI CGRYCG 1 cut(s) 378
BsiHKAI GWGCWC 2 cut(s) 14, 62
BsiSI CCGG 2 cut(s) 297, 345
BsiWI CGTACG 1 cut(s) 153
BslI CCNNNNNNNGG 4 cut(s) 101, 303, 344, 541
BsmI GAATGC 1 cut(s) 40
BsnI GGCC 2 cut(s) 256, 377
Bsp1286I GDGCHC 2 cut(s) 14, 62
Bsp143I GATC 1 cut(s) 91
BspACI CCGC 2 cut(s) 170, 374
BspANI GGCC 2 cut(s) 256, 377
BspCNI CTCAG 1 cut(s) 48
BspMI ACCTGC 1 cut(s) 352
BspPI GGATC 1 cut(s) 86
BsrFI RCCGGY 1 cut(s) 344
BsrI ACTGG 1 cut(s) 610
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 2 cut(s) 39, 556
BssMI GATC 1 cut(s) 91
BssNI GRCGYC 1 cut(s) 300
Bst2UI CCWGG 2 cut(s) 41, 253
Bst4CI ACNGT 3 cut(s) 153, 530, 560
BstACI GRCGYC 1 cut(s) 300
BstBAI YACGTR 1 cut(s) 328
BstDEI CTNAG 2 cut(s) 56, 401
BstDSI CCRYGG 1 cut(s) 556
BstEII GGTNACC 1 cut(s) 511
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 1 cut(s) 94
BstMBI GATC 1 cut(s) 91
BstMCI CGRYCG 1 cut(s) 378
BstMWI GCNNNNNNNGC 2 cut(s) 383, 596
BstNI CCWGG 2 cut(s) 41, 253
BstPAI GACNNNNGTC 1 cut(s) 558
BstPI GGTNACC 1 cut(s) 511
BstSCI CCNGG 2 cut(s) 39, 251
BstSFI CTRYAG 2 cut(s) 76, 178
BstV1I GCAGC 2 cut(s) 73, 608
BstV2I GAAGAC 2 cut(s) 358, 558
BstZI CGGCCG 1 cut(s) 375
BsuRI GGCC 2 cut(s) 256, 377
BtgI CCRYGG 1 cut(s) 556
BveI ACCTGC 1 cut(s) 352
CfoI GCGC 1 cut(s) 177
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 560
CseI GACGC 1 cut(s) 289
Csp6I GTAC 4 cut(s) 18, 81, 154, 427
CviAII CATG 1 cut(s) 622
CviJI RGCY 7 cut(s) 60, 229, 256, 377, 386, 590, 599
CviKI_1 RGCY 7 cut(s) 60, 229, 256, 377, 386, 590, 599
CviQI GTAC 4 cut(s) 18, 81, 154, 427
DdeI CTNAG 2 cut(s) 56, 401
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 375
EagI CGGCCG 1 cut(s) 375
EciI GGCGGA 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 60
EclXI CGGCCG 1 cut(s) 375
Eco24I GRGCYC 1 cut(s) 62
Eco47I GGWCC 1 cut(s) 560
Eco47III AGCGCT 1 cut(s) 176
Eco52I CGGCCG 1 cut(s) 375
Eco53kI GAGCTC 1 cut(s) 60
Eco91I GGTNACC 1 cut(s) 511
EcoICRI GAGCTC 1 cut(s) 60
EcoO65I GGTNACC 1 cut(s) 511
EcoRII CCWGG 2 cut(s) 39, 251
EcoT38I GRGCYC 1 cut(s) 62
FaeI CATG 1 cut(s) 625
FaiI YATR 5 cut(s) 285, 353, 585, 594, 623
FatI CATG 1 cut(s) 621
FblI GTMKAC 2 cut(s) 459, 525
Fnu4HI GCNGC 3 cut(s) 87, 375, 597
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 3 cut(s) 87, 375, 597
FspBI CTAG 2 cut(s) 15, 21
GlaI GCGC 1 cut(s) 176
GluI GCNGC 3 cut(s) 87, 375, 597
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 2 cut(s) 256, 377
HapII CCGG 2 cut(s) 297, 345
HgaI GACGC 1 cut(s) 289
HhaI GCGC 1 cut(s) 177
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 1 cut(s) 625
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 5 cut(s) 195, 391, 487, 626, 642
HpaII CCGG 2 cut(s) 297, 345
HphI GGTGA 4 cut(s) 100, 484, 490, 556
Hpy166II GTNNAC 4 cut(s) 108, 214, 460, 526
Hpy188I TCNGA 1 cut(s) 272
Hpy188III TCNNGA 4 cut(s) 448, 535, 563, 655
Hpy8I GTNNAC 4 cut(s) 108, 214, 460, 526
Hpy99I CGWCG 2 cut(s) 337, 530
HpyAV CCTTC 2 cut(s) 214, 631
HpyCH4III ACNGT 3 cut(s) 153, 530, 560
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 6 cut(s) 30, 145, 361, 397, 503, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 383, 596
HpyF3I CTNAG 2 cut(s) 56, 401
HpySE526I ACGT 1 cut(s) 327
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 1 cut(s) 625
HspAI GCGC 1 cut(s) 175
Kzo9I GATC 1 cut(s) 91
LmnI GCTCC 2 cut(s) 172, 587
Lsp1109I GCAGC 2 cut(s) 73, 608
MaeI CTAG 2 cut(s) 15, 21
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 3 cut(s) 274, 511, 614
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 3 cut(s) 358, 563, 643
MhlI GDGCHC 2 cut(s) 14, 62
MluCI AATT 1 cut(s) 308
MlyI GAGTC 3 cut(s) 204, 385, 635
MmeI TCCRAC 1 cut(s) 272
MnlI CCTC 2 cut(s) 307, 468
MseI TTAA 1 cut(s) 602
MslI CAYNNNNRTG 1 cut(s) 113
MspI CCGG 2 cut(s) 297, 345
MspR9I CCNGG 2 cut(s) 41, 253
Mva1269I GAATGC 1 cut(s) 40
MvaI CCWGG 2 cut(s) 41, 253
MwoI GCNNNNNNNGC 2 cut(s) 383, 596
NdeII GATC 1 cut(s) 91
NlaIII CATG 1 cut(s) 625
OliI CACNNNNGTG 1 cut(s) 113
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 375
PctI GAATGC 1 cut(s) 40
PfeI GAWTC 2 cut(s) 487, 642
Pfl23II CGTACG 1 cut(s) 153
PflFI GACNNNGTC 1 cut(s) 530
PflMI CCANNNNNTGG 1 cut(s) 541
PkrI GCNGC 3 cut(s) 88, 376, 598
PleI GAGTC 3 cut(s) 203, 385, 634
PpsI GAGTC 3 cut(s) 203, 385, 634
Ppu21I YACGTR 1 cut(s) 328
PshAI GACNNNNGTC 1 cut(s) 558
PsiI TTATAA 1 cut(s) 353
Psp124BI GAGCTC 1 cut(s) 62
Psp6I CCWGG 2 cut(s) 39, 251
PspEI GGTNACC 1 cut(s) 511
PspGI CCWGG 2 cut(s) 39, 251
PspLI CGTACG 1 cut(s) 153
PspPI GGNCC 1 cut(s) 560
PsyI GACNNNGTC 1 cut(s) 530
RsaI GTAC 4 cut(s) 19, 82, 155, 428
RsaNI GTAC 4 cut(s) 18, 81, 154, 427
RseI CAYNNNNRTG 1 cut(s) 113
SacI GAGCTC 1 cut(s) 62
SalI GTCGAC 1 cut(s) 524
SaqAI TTAA 1 cut(s) 602
SatI GCNGC 3 cut(s) 87, 375, 597
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 1 cut(s) 560
ScaI AGTACT 2 cut(s) 19, 82
SchI GAGTC 3 cut(s) 204, 385, 635
ScrFI CCNGG 2 cut(s) 41, 253
SduI GDGCHC 2 cut(s) 14, 62
SfcI CTRYAG 2 cut(s) 76, 178
SgrAI CRCCGGYG 1 cut(s) 344
SinI GGWCC 1 cut(s) 560
SmiMI CAYNNNNRTG 1 cut(s) 113
SpeI ACTAGT 2 cut(s) 14, 20
Sse9I AATT 1 cut(s) 308
SsiI CCGC 2 cut(s) 170, 374
SspMI CTAG 2 cut(s) 15, 21
SstI GAGCTC 1 cut(s) 62
StyD4I CCNGG 2 cut(s) 39, 251
TaaI ACNGT 3 cut(s) 153, 530, 560
TaiI ACGT 1 cut(s) 330
TaqI TCGA 4 cut(s) 189, 198, 312, 525
TasI AATT 1 cut(s) 308
TatI WGTACW 3 cut(s) 17, 80, 426
TauI GCSGC 1 cut(s) 377
TfiI GAWTC 2 cut(s) 487, 642
Tru1I TTAA 1 cut(s) 602
Tru9I TTAA 1 cut(s) 602
TseI GCWGC 2 cut(s) 86, 596
TspDTI ATGAA 1 cut(s) 396
Tth111I GACNNNGTC 1 cut(s) 530
Van91I CCANNNNNTGG 1 cut(s) 541
VpaK11BI GGWCC 1 cut(s) 560
XmiI GTMKAC 2 cut(s) 459, 525
XspI CTAG 2 cut(s) 15, 21
ZrmI AGTACT 2 cut(s) 19, 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.