Rh6AG203100

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000532
Physical Location & Seq
Forward (+)
36339012 .. 36340071
1060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG203100.1

Sequence Viewer

Length: 657 bp
ATGAAATCATTCTACTATAAGTTTCTATGTTTGGTCATACTGAGCTTCTTCAACAACCACCATTTCGGTAGTGCTGATCCTACAGATGGGTTCACCCTTGTGCCATTAACAGAAGACAACTTCAAGCTGCAAAAACCATATAACGAACCCCTTGATGATCGATACAGCTACAAAGATGGGGTTCGAAGTTTCTGGATATACAACAATGACAAGCCCTTCAAAACAGACAGTACAACCAGGCCACGCACGGAACTTCGCATAACGGGACATGACTACTCTTCTGGAATTTGGCAATTTGAAGGCTATGCATATGTGCCCAGTGGTACTTCTGGGGTTACAATAGTGCAGATTCATGGTGCAACTGAAGGAGCTACAACTCTACAACTAAGGATGTATGAAGGAGACGGTGGAGATCTCAGATACTACAGATACAACTTAGTTGATACTGATCTCTACGATAAGTGGTTCAGAGTGAACATAATCCACAATGTTGACAAAGGGAAGGTCATAGTTTTCATTGACGGTGTTAAGAAGTTTGTGGTGAAAGATCAGGGACCAGGGGACCTGTACTTCAAATGTGGTGTGTATGCTGCACCATTTAATTCCAGTAATTACATGGAATCAAGGTGGAAGGAGATCAAACTTTACAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

218

Amino Acids

25.37

Weight (kDa)

8.31

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 39 - 216 1.3e-29 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 71
AcsI RAATTY 1 cut(s) 285
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 3 cut(s) 232, 325, 569
AfiI CCNNNNNNNGG 1 cut(s) 86
AgsI TTSAA 5 cut(s) 52, 124, 220, 299, 574
AjnI CCWGG 2 cut(s) 236, 556
AleI CACNNNNGTG 1 cut(s) 98
AluBI AGCT 4 cut(s) 45, 127, 168, 371
AluI AGCT 4 cut(s) 45, 127, 168, 371
Alw26I GTCTC 1 cut(s) 396
AlwI GGATC 1 cut(s) 71
AoxI GGCC 1 cut(s) 239
ApeKI GCWGC 2 cut(s) 127, 590
ApoI RAATTY 1 cut(s) 285
ArsI GACNNNNNNTTYG 2 cut(s) 489, 521
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 554, 562
AsuHPI GGTGA 2 cut(s) 85, 553
AsuII TTCGAA 1 cut(s) 184
AvaII GGWCC 2 cut(s) 554, 562
BaeGI GKGCMC 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 114, 577
BccI CCATC 2 cut(s) 80, 170
BciT130I CCWGG 2 cut(s) 238, 558
BcoDI GTCTC 1 cut(s) 396
BfmI CTRYAG 2 cut(s) 81, 424
BglII AGATCT 1 cut(s) 412
BisI GCNGC 2 cut(s) 128, 591
BlsI GCNGC 2 cut(s) 129, 592
Bme1390I CCNGG 2 cut(s) 238, 558
Bme18I GGWCC 2 cut(s) 554, 562
BmgT120I GGNCC 2 cut(s) 554, 562
BmiI GGNNCC 2 cut(s) 555, 563
BmrFI CCNGG 2 cut(s) 238, 558
BmrI ACTGGG 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 120
Bpu14I TTCGAA 1 cut(s) 184
Bsa29I ATCGAT 1 cut(s) 160
BsaBI GATNNNNATC 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 557
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 318, 606
Bse8I GATNNNNATC 1 cut(s) 447
BseBI CCWGG 2 cut(s) 238, 558
BseCI ATCGAT 1 cut(s) 160
BseDI CCNNGG 1 cut(s) 557
BseGI GGATG 1 cut(s) 396
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMII CTCAG 2 cut(s) 32, 430
BseNI ACTGG 2 cut(s) 318, 606
BseSI GKGCMC 1 cut(s) 318
BseXI GCAGC 2 cut(s) 114, 577
BsgI GTGCAG 2 cut(s) 365, 576
BshFI GGCC 1 cut(s) 241
BshVI ATCGAT 1 cut(s) 160
BslFI GGGAC 3 cut(s) 279, 567, 575
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 1 cut(s) 396
BsmBI CGTCTC 1 cut(s) 396
BsmFI GGGAC 3 cut(s) 279, 567, 575
BsnI GGCC 1 cut(s) 241
Bsp119I TTCGAA 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 6 cut(s) 76, 157, 412, 448, 547, 636
BspANI GGCC 1 cut(s) 241
BspCNI CTCAG 2 cut(s) 33, 429
BspDI ATCGAT 1 cut(s) 160
BspLI GGNNCC 2 cut(s) 555, 563
BspPI GGATC 1 cut(s) 71
BspT104I TTCGAA 1 cut(s) 184
BsrI ACTGG 2 cut(s) 318, 606
BssECI CCNNGG 1 cut(s) 557
BssMI GATC 6 cut(s) 76, 157, 412, 448, 547, 636
Bst2UI CCWGG 2 cut(s) 238, 558
Bst4CI ACNGT 3 cut(s) 230, 407, 524
Bst6I CTCTTC 1 cut(s) 283
BstBI TTCGAA 1 cut(s) 184
BstDEI CTNAG 4 cut(s) 41, 386, 416, 436
BstF5I GGATG 1 cut(s) 396
BstKTI GATC 6 cut(s) 79, 160, 415, 451, 550, 639
BstMAI GTCTC 1 cut(s) 396
BstMBI GATC 6 cut(s) 76, 157, 412, 448, 547, 636
BstNI CCWGG 2 cut(s) 238, 558
BstSCI CCNGG 2 cut(s) 236, 556
BstSFI CTRYAG 2 cut(s) 81, 424
BstSLI GKGCMC 1 cut(s) 318
BstV1I GCAGC 2 cut(s) 114, 577
BstV2I GAAGAC 1 cut(s) 120
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
Bsu15I ATCGAT 1 cut(s) 160
BsuRI GGCC 1 cut(s) 241
BsuTUI ATCGAT 1 cut(s) 160
BtsCI GGATG 1 cut(s) 396
BtsIMutI CAGTG 1 cut(s) 325
Cfr13I GGNCC 2 cut(s) 554, 562
ClaI ATCGAT 1 cut(s) 160
Csp6I GTAC 3 cut(s) 231, 324, 568
CviAII CATG 3 cut(s) 269, 353, 616
CviJI RGCY 7 cut(s) 45, 127, 168, 214, 241, 303, 371
CviKI_1 RGCY 7 cut(s) 45, 127, 168, 214, 241, 303, 371
CviQI GTAC 3 cut(s) 231, 324, 568
DdeI CTNAG 4 cut(s) 41, 386, 416, 436
DpnI GATC 6 cut(s) 78, 159, 414, 450, 549, 638
DpnII GATC 6 cut(s) 76, 157, 412, 448, 547, 636
Eam1104I CTCTTC 1 cut(s) 283
EarI CTCTTC 1 cut(s) 283
Eco47I GGWCC 2 cut(s) 554, 562
Eco57I CTGAAG 1 cut(s) 384
EcoO109I RGGNCCY 1 cut(s) 562
EcoRII CCWGG 2 cut(s) 236, 556
EcoT22I ATGCAT 1 cut(s) 310
Esp3I CGTCTC 1 cut(s) 396
FaeI CATG 3 cut(s) 272, 356, 619
FaqI GGGAC 3 cut(s) 279, 567, 575
FatI CATG 3 cut(s) 268, 352, 615
FauNDI CATATG 1 cut(s) 310
Fnu4HI GCNGC 2 cut(s) 128, 591
FokI GGATG 1 cut(s) 403
Fsp4HI GCNGC 2 cut(s) 128, 591
GluI GCNGC 2 cut(s) 128, 591
HaeIII GGCC 1 cut(s) 241
Hin1II CATG 3 cut(s) 272, 356, 619
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HinfI GANTC 2 cut(s) 349, 620
HphI GGTGA 2 cut(s) 85, 553
Hpy166II GTNNAC 3 cut(s) 93, 475, 493
Hpy188I TCNGA 2 cut(s) 419, 470
Hpy188III TCNNGA 2 cut(s) 193, 282
Hpy8I GTNNAC 3 cut(s) 93, 475, 493
HpyAV CCTTC 6 cut(s) 226, 293, 359, 392, 496, 625
HpyCH4III ACNGT 3 cut(s) 230, 407, 524
HpyCH4V TGCA 5 cut(s) 130, 308, 346, 359, 593
HpyF3I CTNAG 4 cut(s) 41, 386, 416, 436
Hsp92II CATG 3 cut(s) 272, 356, 619
Kzo9I GATC 6 cut(s) 76, 157, 412, 448, 547, 636
LmnI GCTCC 1 cut(s) 368
Lsp1109I GCAGC 2 cut(s) 114, 577
MaeIII GTNAC 1 cut(s) 334
MalI GATC 6 cut(s) 78, 159, 414, 450, 549, 638
MboI GATC 6 cut(s) 76, 157, 412, 448, 547, 636
MboII GAAGA 3 cut(s) 40, 125, 270
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 1 cut(s) 318
MluCI AATT 4 cut(s) 285, 293, 601, 610
Mph1103I ATGCAT 1 cut(s) 310
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 3 cut(s) 107, 528, 600
MslI CAYNNNNRTG 1 cut(s) 98
MspR9I CCNGG 2 cut(s) 238, 558
MvaI CCWGG 2 cut(s) 238, 558
NdeI CATATG 1 cut(s) 310
NdeII GATC 6 cut(s) 76, 157, 412, 448, 547, 636
NlaIII CATG 3 cut(s) 272, 356, 619
NlaIV GGNNCC 2 cut(s) 555, 563
NsiI ATGCAT 1 cut(s) 310
NspV TTCGAA 1 cut(s) 184
OliI CACNNNNGTG 1 cut(s) 98
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 349, 620
PkrI GCNGC 2 cut(s) 129, 592
PpuMI RGGWCCY 1 cut(s) 562
Psp5II RGGWCCY 1 cut(s) 562
Psp6I CCWGG 2 cut(s) 236, 556
PspGI CCWGG 2 cut(s) 236, 556
PspN4I GGNNCC 2 cut(s) 555, 563
PspPI GGNCC 2 cut(s) 554, 562
PspPPI RGGWCCY 1 cut(s) 562
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 3 cut(s) 232, 325, 569
RsaNI GTAC 3 cut(s) 231, 324, 568
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 3 cut(s) 107, 528, 600
SatI GCNGC 2 cut(s) 128, 591
Sau3AI GATC 6 cut(s) 76, 157, 412, 448, 547, 636
Sau96I GGNCC 2 cut(s) 554, 562
ScrFI CCNGG 2 cut(s) 238, 558
SduI GDGCHC 1 cut(s) 318
SetI ASST 7 cut(s) 47, 129, 170, 373, 507, 567, 629
SfcI CTRYAG 2 cut(s) 81, 424
SfuI TTCGAA 1 cut(s) 184
SinI GGWCC 2 cut(s) 554, 562
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 4 cut(s) 285, 293, 601, 610
StyD4I CCNGG 2 cut(s) 236, 556
TaaI ACNGT 3 cut(s) 230, 407, 524
TaqI TCGA 2 cut(s) 160, 184
TasI AATT 4 cut(s) 285, 293, 601, 610
TatI WGTACW 2 cut(s) 230, 567
TfiI GAWTC 2 cut(s) 349, 620
Tru1I TTAA 3 cut(s) 107, 528, 600
Tru9I TTAA 3 cut(s) 107, 528, 600
TscAI CASTG 1 cut(s) 325
TseI GCWGC 2 cut(s) 127, 590
TspDTI ATGAA 4 cut(s) 17, 341, 411, 505
TspGWI ACGGA 1 cut(s) 263
TspRI CASTG 1 cut(s) 325
VpaK11BI GGWCC 2 cut(s) 554, 562
XapI RAATTY 1 cut(s) 285
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
XmnI GAANNNNTTC 1 cut(s) 8
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.