MD10G1030500.v1.1

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000532
Physical Location & Seq
Reverse (-)
4015943 .. 4016936
994 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1030500.v1.1.491

Sequence Viewer

Length: 666 bp
ATGAAAAGCATCATCAGTTCTTGTAACTTCAAAAAGGTTGTGTTTTTGCTACAGATCATCTTCTTCGTTGAAGCCCAACATTTCTGTTGTGCTGATCCCACTTCTGGATTCACACCTGTGCATTTCACTGCACAAAATCGAAAGTTGCAGAAGCCGTATAACAAGTCTCCTCATGAGCGTTACGGTAATAAAGATGGAGTTGAAAAGTTTTGGATCTACAACCACGACAAGCCCTTTCAAAAGCACAATCCAACCAGGCCACGCTCCGAAATCAGAATTTCTGGGCATGACTACACTTCCGGGGTTTGGCAATTTGAAGGCAATTTTCTTGTCCCACAAGGCACCTCAGGGGTCACAATAATGCAGATATTTGGTTCATCCAAACAAGCGACAGCTCTGCAACTGAGGGTCTATGATGGGGATTTGAAGTACTATGCTCACAATGTGGTAGCCCCTAATATCTACGATAAGTGGGTTAGGCTGAATGTGATTCACAACGTTGCTGCAGGGAAAGTGACAATTTTTATCGACGGCAAACGGAAGTTTGTCGCCAGAGATCACGGCCGAGCAACCTTTTACTTCAAGTATGGAGTTTATGGAGCCTTATCTCGTTCAAGTAACTACATGCAATCAAGTTGGAGAGGAATCAAACTTTTCAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

222

Amino Acids

25.5

Weight (kDa)

9.9

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 48 - 219 6.5e-26 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 341
AclI AACGTT 1 cut(s) 498
AclWI GGATC 2 cut(s) 89, 221
AcoI YGGCCR 1 cut(s) 562
AcsI RAATTY 1 cut(s) 276
AdeI CACNNNGTG 1 cut(s) 445
AfaI GTAC 1 cut(s) 431
AfiI CCNNNNNNNGG 2 cut(s) 104, 306
AgsI TTSAA 9 cut(s) 31, 71, 203, 239, 317, 427, 583, 615, 658
AjnI CCWGG 1 cut(s) 254
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 1 cut(s) 395
AluI AGCT 1 cut(s) 395
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 2 cut(s) 89, 221
AoxI GGCC 2 cut(s) 257, 562
ApeKI GCWGC 1 cut(s) 503
ApoI RAATTY 1 cut(s) 276
AsuC2I CCSGG 1 cut(s) 301
AxyI CCTNAGG 1 cut(s) 346
BanI GGYRCC 1 cut(s) 341
BbvI GCAGC 1 cut(s) 490
BccI CCATC 2 cut(s) 188, 410
BceAI ACGGC 3 cut(s) 139, 547, 577
BcgI CGANNNNNNTGC 2 cut(s) 379, 413
BciT130I CCWGG 1 cut(s) 256
BcnI CCSGG 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 171
BfmI CTRYAG 2 cut(s) 50, 504
BisI GCNGC 1 cut(s) 504
BlsI GCNGC 1 cut(s) 505
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 2 cut(s) 256, 301
BmiI GGNNCC 2 cut(s) 343, 601
BmrFI CCNGG 2 cut(s) 256, 301
BmsI GCATC 1 cut(s) 18
BpuMI CCSGG 1 cut(s) 301
BsaJI CCNNGG 1 cut(s) 300
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 306
Bse21I CCTNAGG 1 cut(s) 346
BseBI CCWGG 1 cut(s) 256
BseDI CCNNGG 1 cut(s) 300
BseGI GGATG 1 cut(s) 377
BseLI CCNNNNNNNGG 2 cut(s) 104, 306
BseMII CTCAG 2 cut(s) 360, 395
BseRI GAGGAG 1 cut(s) 159
BseX3I CGGCCG 1 cut(s) 562
BseXI GCAGC 1 cut(s) 490
BsgI GTGCAG 1 cut(s) 114
Bsh1285I CGRYCG 1 cut(s) 565
BshFI GGCC 2 cut(s) 259, 564
BshNI GGYRCC 1 cut(s) 341
BsiEI CGRYCG 1 cut(s) 565
BsiSI CCGG 1 cut(s) 300
BslFI GGGAC 1 cut(s) 317
BslI CCNNNNNNNGG 2 cut(s) 104, 306
BsmAI GTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 317
BsnI GGCC 2 cut(s) 259, 564
Bsp143I GATC 4 cut(s) 54, 94, 213, 556
BspANI GGCC 2 cut(s) 259, 564
BspCNI CTCAG 2 cut(s) 359, 396
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 2 cut(s) 343, 601
BspMAI CTGCAG 1 cut(s) 508
BspPI GGATC 2 cut(s) 89, 221
BspT107I GGYRCC 1 cut(s) 341
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 4 cut(s) 54, 94, 213, 556
Bst2UI CCWGG 1 cut(s) 256
Bst4CI ACNGT 1 cut(s) 185
BstDEI CTNAG 2 cut(s) 346, 404
BstF5I GGATG 1 cut(s) 377
BstKTI GATC 4 cut(s) 57, 97, 216, 559
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 4 cut(s) 54, 94, 213, 556
BstMCI CGRYCG 1 cut(s) 565
BstNI CCWGG 1 cut(s) 256
BstNSI RCATGY 1 cut(s) 628
BstSCI CCNGG 2 cut(s) 254, 299
BstSFI CTRYAG 2 cut(s) 50, 504
BstV1I GCAGC 1 cut(s) 490
BstX2I RGATCY 1 cut(s) 213
BstYI RGATCY 1 cut(s) 213
BstZI CGGCCG 1 cut(s) 562
Bsu36I CCTNAGG 1 cut(s) 346
BsuRI GGCC 2 cut(s) 259, 564
BtsCI GGATG 1 cut(s) 377
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 126
CciI TCATGA 1 cut(s) 172
Csp6I GTAC 1 cut(s) 430
CviAII CATG 3 cut(s) 173, 287, 625
CviJI RGCY 9 cut(s) 74, 154, 232, 259, 395, 452, 481, 564, 602
CviKI_1 RGCY 9 cut(s) 74, 154, 232, 259, 395, 452, 481, 564, 602
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 2 cut(s) 346, 404
DpnI GATC 4 cut(s) 56, 96, 215, 558
DpnII GATC 4 cut(s) 54, 94, 213, 556
DraIII CACNNNGTG 1 cut(s) 445
EaeI YGGCCR 1 cut(s) 562
EagI CGGCCG 1 cut(s) 562
EclXI CGGCCG 1 cut(s) 562
Eco52I CGGCCG 1 cut(s) 562
Eco81I CCTNAGG 1 cut(s) 346
EcoRII CCWGG 1 cut(s) 254
FaeI CATG 3 cut(s) 176, 290, 628
FaiI YATR 8 cut(s) 159, 174, 288, 414, 435, 588, 597, 626
FaqI GGGAC 1 cut(s) 317
FatI CATG 3 cut(s) 172, 286, 624
Fnu4HI GCNGC 1 cut(s) 504
FokI GGATG 1 cut(s) 364
Fsp4HI GCNGC 1 cut(s) 504
GluI GCNGC 1 cut(s) 504
HaeIII GGCC 2 cut(s) 259, 564
HapII CCGG 1 cut(s) 300
Hin1II CATG 3 cut(s) 176, 290, 628
HinfI GANTC 3 cut(s) 108, 490, 645
HpaII CCGG 1 cut(s) 300
Hpy188I TCNGA 2 cut(s) 268, 275
Hpy188III TCNNGA 2 cut(s) 105, 173
Hpy99I CGWCG 1 cut(s) 533
HpyAV CCTTC 1 cut(s) 311
HpyCH4III ACNGT 1 cut(s) 185
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 7 cut(s) 121, 131, 148, 364, 400, 506, 628
HpyF3I CTNAG 2 cut(s) 346, 404
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 3 cut(s) 176, 290, 628
Kzo9I GATC 4 cut(s) 54, 94, 213, 556
LmnI GCTCC 2 cut(s) 269, 599
LpnPI CCDG 9 cut(s) 90, 129, 241, 267, 268, 313, 333, 492, 565
Lsp1109I GCAGC 1 cut(s) 490
LweI GCATC 1 cut(s) 18
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 5 cut(s) 23, 179, 352, 514, 617
MalI GATC 4 cut(s) 56, 96, 215, 558
MboI GATC 4 cut(s) 54, 94, 213, 556
MboII GAAGA 2 cut(s) 52, 55
MflI RGATCY 1 cut(s) 213
MluCI AATT 4 cut(s) 276, 311, 322, 519
MmeI TCCRAC 2 cut(s) 275, 617
MnlI CCTC 4 cut(s) 180, 355, 399, 635
MslI CAYNNNNRTG 2 cut(s) 116, 359
MspI CCGG 1 cut(s) 300
MspR9I CCNGG 2 cut(s) 256, 301
MvaI CCWGG 1 cut(s) 256
NciI CCSGG 1 cut(s) 301
NdeII GATC 4 cut(s) 54, 94, 213, 556
NlaIII CATG 3 cut(s) 176, 290, 628
NlaIV GGNNCC 2 cut(s) 343, 601
NmeAIII GCCGAG 1 cut(s) 590
NmuCI GTSAC 2 cut(s) 352, 514
NspI RCATGY 1 cut(s) 628
OliI CACNNNNGTG 1 cut(s) 116
PagI TCATGA 1 cut(s) 172
PfeI GAWTC 3 cut(s) 108, 490, 645
PkrI GCNGC 1 cut(s) 505
Psp1406I AACGTT 1 cut(s) 498
Psp6I CCWGG 1 cut(s) 254
PspGI CCWGG 1 cut(s) 254
PspN4I GGNNCC 2 cut(s) 343, 601
PstI CTGCAG 1 cut(s) 508
PsuI RGATCY 1 cut(s) 213
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
RseI CAYNNNNRTG 2 cut(s) 116, 359
SatI GCNGC 1 cut(s) 504
Sau3AI GATC 4 cut(s) 54, 94, 213, 556
ScaI AGTACT 1 cut(s) 431
ScrFI CCNGG 2 cut(s) 256, 301
SetI ASST 6 cut(s) 39, 118, 347, 397, 501, 575
SfaNI GCATC 1 cut(s) 18
SfcI CTRYAG 2 cut(s) 50, 504
SmiMI CAYNNNNRTG 2 cut(s) 116, 359
Sse9I AATT 4 cut(s) 276, 311, 322, 519
StyD4I CCNGG 2 cut(s) 254, 299
TaaI ACNGT 1 cut(s) 185
TaiI ACGT 1 cut(s) 501
TaqI TCGA 2 cut(s) 139, 528
TasI AATT 4 cut(s) 276, 311, 322, 519
TatI WGTACW 1 cut(s) 429
TfiI GAWTC 3 cut(s) 108, 490, 645
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 2 cut(s) 352, 514
TseI GCWGC 1 cut(s) 503
Tsp45I GTSAC 2 cut(s) 352, 514
TspDTI ATGAA 2 cut(s) 17, 366
TspGWI ACGGA 1 cut(s) 553
TspRI CASTG 1 cut(s) 133
XapI RAATTY 1 cut(s) 276
XceI RCATGY 1 cut(s) 628
ZrmI AGTACT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.