Rw2G022490

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000532
Physical Location & Seq
Forward (+)
31969780 .. 31970721
942 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G022490.1

Sequence Viewer

Length: 660 bp
ATGAGTGTCCTTAATCACATTGCTTTAGTAAGCACAGCTTTTATTCTTCTGCTAACGCAATGGTCAAGCAGCTGGCCTGTAGATCCAACCCTTGGCTTCATAAGTCTCCCATTAAACACATCAAACTATCATATCCACAAGCCATATGACTTGCCGATAAGTAAACGATACAGTTTCAAACATGGAGTGCACAAGATGTGGGTGTACTTCAAGGATAAGCCCCTCTCTCGAAATAGTCCTACATATCCTCGAACAGAGATCCGAATAAGGGGATACGATTATTCTTCAGGTGTATGGCAATTCGAAGGATACGGGTACGTACCATGTGGAACAGCCAATGTGACCATAATGCAAGTGTTTGGGGCAAGCCCTCACGCAACCACTCTAATGCTTAGAGTTGGTTCAAATGGTACCCTTACATACTATAGGACTGCAGTCCTGGATCCTTACATCTATGACAGATGGTTCCGAGTTAATGTAATCCACGATGTCTACGCCTCGCATTTGAAGGTTTATATCGATGGAGTTCTCAAATTAGAGGCTCCCGGTCGCGGAGGAAAGTTTCATTATTTTAAATGCGGCGTCTACTCTCAGCCCGATGGTTCTTACTACATGGAGTCTCGTTGGAAAGGGATCAAGGTGCTGAAAAAATGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

219

Amino Acids

25.2

Weight (kDa)

9.48

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 39 - 214 5e-26 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 410
AccB1I GGYRCC 1 cut(s) 410
AccB7I CCANNNNNTGG 1 cut(s) 92
AccI GTMKAC 2 cut(s) 492, 585
AccII CGCG 1 cut(s) 552
AciI CCGC 2 cut(s) 552, 579
AclWI GGATC 5 cut(s) 77, 253, 437, 450, 641
AcuI CTGAAG 1 cut(s) 270
AcyI GRCGYC 1 cut(s) 582
AfaI GTAC 4 cut(s) 206, 317, 321, 412
AfiI CCNNNNNNNGG 3 cut(s) 92, 268, 551
AgsI TTSAA 4 cut(s) 178, 211, 405, 508
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 2 cut(s) 38, 72
AluI AGCT 2 cut(s) 38, 72
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 2 cut(s) 110, 624
Alw44I GTGCAC 1 cut(s) 188
AlwI GGATC 5 cut(s) 77, 253, 437, 450, 641
AoxI GGCC 1 cut(s) 74
ApaLI GTGCAC 1 cut(s) 188
ApeKI GCWGC 1 cut(s) 69
Asp718I GGTACC 1 cut(s) 410
AsuC2I CCSGG 1 cut(s) 546
AsuII TTCGAA 1 cut(s) 303
BaeGI GKGCMC 1 cut(s) 192
BamHI GGATCC 1 cut(s) 442
BanI GGYRCC 1 cut(s) 410
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 1 cut(s) 81
BccI CCATC 3 cut(s) 456, 515, 593
BciT130I CCWGG 1 cut(s) 440
BciVI GTATCC 2 cut(s) 266, 302
BcnI CCSGG 1 cut(s) 546
BcoDI GTCTC 2 cut(s) 110, 624
BfmI CTRYAG 3 cut(s) 78, 424, 432
BfuI GTATCC 2 cut(s) 266, 302
BisI GCNGC 2 cut(s) 70, 580
BlsI GCNGC 2 cut(s) 71, 581
Bme1390I CCNGG 2 cut(s) 440, 546
BmiI GGNNCC 4 cut(s) 412, 444, 467, 543
BmrFI CCNGG 2 cut(s) 440, 546
BoxI GACNNNNGTC 1 cut(s) 434
Bpu14I TTCGAA 1 cut(s) 303
BpuMI CCSGG 1 cut(s) 546
Bsa29I ATCGAT 1 cut(s) 519
BsaAI YACGTR 1 cut(s) 319
BsaHI GRCGYC 1 cut(s) 582
BsaJI CCNNGG 1 cut(s) 91
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 268, 551
Bse3DI GCAATG 2 cut(s) 18, 65
BseBI CCWGG 1 cut(s) 440
BseCI ATCGAT 1 cut(s) 519
BseDI CCNNGG 1 cut(s) 91
BseLI CCNNNNNNNGG 3 cut(s) 92, 268, 551
BseMI GCAATG 2 cut(s) 18, 65
BseMII CTCAG 1 cut(s) 605
BseSI GKGCMC 1 cut(s) 192
BseXI GCAGC 1 cut(s) 81
Bsh1236I CGCG 1 cut(s) 552
Bsh1285I CGRYCG 1 cut(s) 550
BshFI GGCC 1 cut(s) 76
BshNI GGYRCC 1 cut(s) 410
BshVI ATCGAT 1 cut(s) 519
BsiEI CGRYCG 1 cut(s) 550
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 1 cut(s) 546
BslI CCNNNNNNNGG 3 cut(s) 92, 268, 551
BsmAI GTCTC 2 cut(s) 110, 624
BsnI GGCC 1 cut(s) 76
Bsp119I TTCGAA 1 cut(s) 303
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 4 cut(s) 82, 258, 442, 633
BspACI CCGC 2 cut(s) 552, 579
BspANI GGCC 1 cut(s) 76
BspCNI CTCAG 1 cut(s) 604
BspDI ATCGAT 1 cut(s) 519
BspFNI CGCG 1 cut(s) 552
BspLI GGNNCC 4 cut(s) 412, 444, 467, 543
BspMAI CTGCAG 1 cut(s) 436
BspPI GGATC 5 cut(s) 77, 253, 437, 450, 641
BspT104I TTCGAA 1 cut(s) 303
BspT107I GGYRCC 1 cut(s) 410
BsrDI GCAATG 2 cut(s) 18, 65
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 4 cut(s) 82, 258, 442, 633
BssNI GRCGYC 1 cut(s) 582
BssT1I CCWWGG 1 cut(s) 91
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 1 cut(s) 173
BstACI GRCGYC 1 cut(s) 582
BstBAI YACGTR 1 cut(s) 319
BstBI TTCGAA 1 cut(s) 303
BstC8I GCNNGC 2 cut(s) 74, 367
BstDEI CTNAG 2 cut(s) 392, 591
BstFNI CGCG 1 cut(s) 552
BstKTI GATC 4 cut(s) 85, 261, 445, 636
BstMAI GTCTC 2 cut(s) 110, 624
BstMBI GATC 4 cut(s) 82, 258, 442, 633
BstMCI CGRYCG 1 cut(s) 550
BstNI CCWGG 1 cut(s) 440
BstPAI GACNNNNGTC 1 cut(s) 434
BstSCI CCNGG 2 cut(s) 438, 544
BstSFI CTRYAG 3 cut(s) 78, 424, 432
BstSLI GKGCMC 1 cut(s) 192
BstSNI TACGTA 1 cut(s) 319
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 1 cut(s) 81
BstX2I RGATCY 3 cut(s) 82, 258, 442
BstYI RGATCY 3 cut(s) 82, 258, 442
Bsu15I ATCGAT 1 cut(s) 519
BsuI GTATCC 2 cut(s) 266, 302
BsuRI GGCC 1 cut(s) 76
BsuTUI ATCGAT 1 cut(s) 519
Cac8I GCNNGC 2 cut(s) 74, 367
ClaI ATCGAT 1 cut(s) 519
CseI GACGC 1 cut(s) 571
Csp6I GTAC 4 cut(s) 205, 316, 320, 411
CviAII CATG 3 cut(s) 182, 324, 613
CviQI GTAC 4 cut(s) 205, 316, 320, 411
DdeI CTNAG 2 cut(s) 392, 591
DpnI GATC 4 cut(s) 84, 260, 444, 635
DpnII GATC 4 cut(s) 82, 258, 442, 633
DraI TTTAAA 1 cut(s) 574
Eco105I TACGTA 1 cut(s) 319
Eco130I CCWWGG 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 270
EcoRII CCWGG 1 cut(s) 438
EcoT14I CCWWGG 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 3 cut(s) 185, 327, 616
FalI AAGNNNNNCTT 2 cut(s) 22, 54
FatI CATG 3 cut(s) 181, 323, 612
FauNDI CATATG 1 cut(s) 145
FblI GTMKAC 2 cut(s) 492, 585
Fnu4HI GCNGC 2 cut(s) 70, 580
Fsp4HI GCNGC 2 cut(s) 70, 580
GluI GCNGC 2 cut(s) 70, 580
HaeIII GGCC 1 cut(s) 76
HapII CCGG 1 cut(s) 546
HgaI GACGC 1 cut(s) 571
Hin1I GRCGYC 1 cut(s) 582
Hin1II CATG 3 cut(s) 185, 327, 616
HinfI GANTC 1 cut(s) 617
HpaII CCGG 1 cut(s) 546
Hpy166II GTNNAC 5 cut(s) 164, 190, 205, 493, 586
Hpy188I TCNGA 2 cut(s) 263, 470
Hpy188III TCNNGA 1 cut(s) 228
Hpy8I GTNNAC 5 cut(s) 164, 190, 205, 493, 586
HpyAV CCTTC 2 cut(s) 299, 502
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 3 cut(s) 190, 352, 434
HpyF3I CTNAG 2 cut(s) 392, 591
HpySE526I ACGT 1 cut(s) 318
Hsp92I GRCGYC 1 cut(s) 582
Hsp92II CATG 3 cut(s) 185, 327, 616
KpnI GGTACC 1 cut(s) 414
Kzo9I GATC 4 cut(s) 82, 258, 442, 633
LmnI GCTCC 1 cut(s) 547
LpnPI CCDG 6 cut(s) 58, 90, 273, 425, 452, 559
Lsp1109I GCAGC 1 cut(s) 81
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 2 cut(s) 340, 653
MalI GATC 4 cut(s) 84, 260, 444, 635
MboI GATC 4 cut(s) 82, 258, 442, 633
MboII GAAGA 2 cut(s) 38, 276
MflI RGATCY 3 cut(s) 82, 258, 442
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 2 cut(s) 299, 533
MlyI GAGTC 1 cut(s) 626
MmeI TCCRAC 2 cut(s) 110, 605
MnlI CCTC 6 cut(s) 233, 258, 381, 508, 532, 548
MseI TTAA 4 cut(s) 12, 113, 474, 573
MslI CAYNNNNRTG 1 cut(s) 386
MspA1I CMGCKG 1 cut(s) 72
MspI CCGG 1 cut(s) 546
MspR9I CCNGG 2 cut(s) 440, 546
MvaI CCWGG 1 cut(s) 440
MvnI CGCG 1 cut(s) 552
NciI CCSGG 1 cut(s) 546
NdeI CATATG 1 cut(s) 145
NdeII GATC 4 cut(s) 82, 258, 442, 633
NlaIII CATG 3 cut(s) 185, 327, 616
NlaIV GGNNCC 4 cut(s) 412, 444, 467, 543
NmuCI GTSAC 2 cut(s) 340, 653
NspV TTCGAA 1 cut(s) 303
PflMI CCANNNNNTGG 1 cut(s) 92
PfoI TCCNGGA 1 cut(s) 438
PkrI GCNGC 2 cut(s) 71, 581
PleI GAGTC 1 cut(s) 625
PpsI GAGTC 1 cut(s) 625
Ppu21I YACGTR 1 cut(s) 319
PshAI GACNNNNGTC 1 cut(s) 434
Psp6I CCWGG 1 cut(s) 438
PspGI CCWGG 1 cut(s) 438
PspN4I GGNNCC 4 cut(s) 412, 444, 467, 543
PstI CTGCAG 1 cut(s) 436
PsuI RGATCY 3 cut(s) 82, 258, 442
PvuII CAGCTG 1 cut(s) 72
RsaI GTAC 4 cut(s) 206, 317, 321, 412
RsaNI GTAC 4 cut(s) 205, 316, 320, 411
RseI CAYNNNNRTG 1 cut(s) 386
SaqAI TTAA 4 cut(s) 12, 113, 474, 573
SatI GCNGC 2 cut(s) 70, 580
Sau3AI GATC 4 cut(s) 82, 258, 442, 633
SchI GAGTC 1 cut(s) 626
ScrFI CCNGG 2 cut(s) 440, 546
SduI GDGCHC 1 cut(s) 192
SetI ASST 6 cut(s) 40, 74, 292, 321, 513, 642
SfcI CTRYAG 3 cut(s) 78, 424, 432
SfuI TTCGAA 1 cut(s) 303
SmiMI CAYNNNNRTG 1 cut(s) 386
SnaBI TACGTA 1 cut(s) 319
Sse9I AATT 2 cut(s) 299, 533
SsiI CCGC 2 cut(s) 552, 579
StyD4I CCNGG 2 cut(s) 438, 544
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 1 cut(s) 173
TaiI ACGT 1 cut(s) 321
TaqI TCGA 4 cut(s) 229, 250, 303, 519
TasI AATT 2 cut(s) 299, 533
TatI WGTACW 1 cut(s) 204
TauI GCSGC 1 cut(s) 582
Tru1I TTAA 4 cut(s) 12, 113, 474, 573
Tru9I TTAA 4 cut(s) 12, 113, 474, 573
TseFI GTSAC 2 cut(s) 340, 653
TseI GCWGC 1 cut(s) 69
Tsp45I GTSAC 2 cut(s) 340, 653
TspDTI ATGAA 2 cut(s) 88, 554
Van91I CCANNNNNTGG 1 cut(s) 92
VneI GTGCAC 1 cut(s) 188
XmiI GTMKAC 2 cut(s) 492, 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.