RLG00000018489

Alginate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000532
Physical Location & Seq
Forward (+)
30674022 .. 30675007
986 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018489

Sequence Viewer

Length: 684 bp
ATGGCATACTCCTTTCACTCCCTAGCTTTCTTCTGCTCAAGCATTCTAATATTCAAGCTTATCAGTCTTCACCAGTCAGCCTTGGCTTGGAGTTGGGGACCTGATGTTCCTCCTACACAGGGCTTTGTCTCCCTCCCTTTCAACCGATCCTACTACCATATTCAGAAACCATATGATGTGCCTGAAGACCAGCGCTACTGCTTCATCGATGGAGTTCATAAATGTTGGGTCTACTCCACAGACAAACCTCACACTACCACTAGCCAAACCCTGCCTCGCACTGAAATTGCTATACAAGGATACGGTTACTCTTCTGGTGTGTGGGAATTTGAAGCATATGCGTATGTGCCAAATGGAACATCAGGGGTCTGCATAATGCAAGTGTTTGGAGCAACTGCTCCTCATGCCTCAACTCTGATGTTGAGGGTCTACAATGGTTCACTCACCTACTACAGGGCTCCAGTACTGGTTCCAAACATCTACGATAAATGGTTCAAGATCAATGTGGTTCACGACGTTGATGCTGCGAAAGTCAGAGTCTTCATTGACGGCTGTCTCAAAATCGAGGCAGATGGTCGTGGAGGAACGTCTCATGCTTTCAAATGTGGAGTGTATGCCCAGATGAATGACTCTACTTACATGGAGTCTCGCTGGAAAGACATCAAAGTTTTAAGGAAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

228

Amino Acids

25.75

Weight (kDa)

8.39

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 50 - 223 3.2e-26 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 231, 429
AclWI GGATC 1 cut(s) 141
AcsI RAATTY 1 cut(s) 326
AcuI CTGAAG 1 cut(s) 204
AfaI GTAC 1 cut(s) 465
AfeI AGCGCT 1 cut(s) 194
AfiI CCNNNNNNNGG 3 cut(s) 87, 119, 453
AgsI TTSAA 5 cut(s) 55, 142, 332, 496, 601
AloI GAACNNNNNNTCC 2 cut(s) 90, 122
AluBI AGCT 2 cut(s) 26, 58
AluI AGCT 2 cut(s) 26, 58
Alw26I GTCTC 4 cut(s) 133, 560, 594, 651
AlwI GGATC 1 cut(s) 141
Aor51HI AGCGCT 1 cut(s) 194
ApeKI GCWGC 1 cut(s) 524
ApoI RAATTY 1 cut(s) 326
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 1 cut(s) 98
AsuHPI GGTGA 2 cut(s) 62, 436
AvaII GGWCC 1 cut(s) 98
BanII GRGCYC 1 cut(s) 460
BbsI GAAGAC 3 cut(s) 59, 192, 532
BbvI GCAGC 1 cut(s) 511
BccI CCATC 2 cut(s) 203, 566
BceAI ACGGC 1 cut(s) 565
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciVI GTATCC 1 cut(s) 293
BcoDI GTCTC 4 cut(s) 133, 560, 594, 651
BfaI CTAG 2 cut(s) 23, 261
BfmI CTRYAG 1 cut(s) 451
BfoI RGCGCY 1 cut(s) 196
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 1 cut(s) 525
BlsI GCNGC 1 cut(s) 526
BmcAI AGTACT 1 cut(s) 465
Bme18I GGWCC 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 3 cut(s) 99, 459, 471
BmsI GCATC 1 cut(s) 511
BoxI GACNNNNGTC 1 cut(s) 552
BpiI GAAGAC 3 cut(s) 59, 192, 532
BpmI CTGGAG 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 22
Bsa29I ATCGAT 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 81
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 119, 453
Bse1I ACTGG 3 cut(s) 73, 461, 471
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 81
BseLI CCNNNNNNNGG 3 cut(s) 87, 119, 453
BseNI ACTGG 3 cut(s) 73, 461, 471
BseRI GAGGAG 1 cut(s) 390
BseXI GCAGC 1 cut(s) 511
BshVI ATCGAT 1 cut(s) 207
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 3 cut(s) 87, 119, 453
BsmAI GTCTC 4 cut(s) 133, 560, 594, 651
BsmBI CGTCTC 1 cut(s) 594
BsmFI GGGAC 1 cut(s) 111
BsmI GAATGC 1 cut(s) 42
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 2 cut(s) 146, 498
BspDI ATCGAT 1 cut(s) 207
BspLI GGNNCC 3 cut(s) 99, 459, 471
BspPI GGATC 1 cut(s) 141
BsrI ACTGG 3 cut(s) 73, 461, 471
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 2 cut(s) 146, 498
BssT1I CCWWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 1 cut(s) 316
BstENI CCTNNNNNAGG 1 cut(s) 451
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 2 cut(s) 149, 501
BstMAI GTCTC 4 cut(s) 133, 560, 594, 651
BstMBI GATC 2 cut(s) 146, 498
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstPAI GACNNNNGTC 1 cut(s) 552
BstSFI CTRYAG 1 cut(s) 451
BstV1I GCAGC 1 cut(s) 511
BstV2I GAAGAC 3 cut(s) 59, 192, 532
Bsu15I ATCGAT 1 cut(s) 207
BsuI GTATCC 1 cut(s) 293
BsuTUI ATCGAT 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 279
CfoI GCGC 1 cut(s) 195
Cfr13I GGNCC 1 cut(s) 98
ClaI ATCGAT 1 cut(s) 207
Csp6I GTAC 1 cut(s) 464
CviAII CATG 3 cut(s) 404, 593, 640
CviJI RGCY 8 cut(s) 26, 58, 80, 86, 123, 264, 458, 552
CviKI_1 RGCY 8 cut(s) 26, 58, 80, 86, 123, 264, 458, 552
CviQI GTAC 1 cut(s) 464
DpnI GATC 2 cut(s) 148, 500
DpnII GATC 2 cut(s) 146, 498
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 460
Eco47I GGWCC 1 cut(s) 98
Eco47III AGCGCT 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 204
EcoNI CCTNNNNNAGG 1 cut(s) 451
EcoO109I RGGNCCY 1 cut(s) 98
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 1 cut(s) 460
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 594
FaeI CATG 3 cut(s) 407, 596, 643
FaqI GGGAC 1 cut(s) 111
FatI CATG 3 cut(s) 403, 592, 639
FauNDI CATATG 2 cut(s) 172, 337
FblI GTMKAC 2 cut(s) 231, 429
Fnu4HI GCNGC 1 cut(s) 525
FriOI GRGCYC 1 cut(s) 460
Fsp4HI GCNGC 1 cut(s) 525
FspBI CTAG 2 cut(s) 23, 261
GlaI GCGC 1 cut(s) 194
GluI GCNGC 1 cut(s) 525
GsuI CTGGAG 1 cut(s) 444
HaeII RGCGCY 1 cut(s) 196
HhaI GCGC 1 cut(s) 195
Hin1II CATG 3 cut(s) 407, 596, 643
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 3 cut(s) 537, 629, 644
HphI GGTGA 2 cut(s) 62, 436
Hpy166II GTNNAC 4 cut(s) 232, 430, 440, 511
Hpy188I TCNGA 3 cut(s) 165, 417, 536
Hpy188III TCNNGA 2 cut(s) 496, 512
Hpy8I GTNNAC 4 cut(s) 232, 430, 440, 511
Hpy99I CGWCG 1 cut(s) 518
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 2 cut(s) 516, 587
HpyCH4V TGCA 2 cut(s) 372, 379
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
HpySE526I ACGT 2 cut(s) 516, 587
Hsp92II CATG 3 cut(s) 407, 596, 643
HspAI GCGC 1 cut(s) 193
Kzo9I GATC 2 cut(s) 146, 498
LmnI GCTCC 3 cut(s) 389, 403, 463
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 1 cut(s) 511
MaeI CTAG 2 cut(s) 23, 261
MaeII ACGT 2 cut(s) 516, 587
MaeIII GTNAC 1 cut(s) 305
MalI GATC 2 cut(s) 148, 500
MboI GATC 2 cut(s) 146, 498
MboII GAAGA 5 cut(s) 22, 59, 197, 303, 532
MhlI GDGCHC 1 cut(s) 460
MluCI AATT 2 cut(s) 285, 326
MlyI GAGTC 3 cut(s) 546, 623, 653
MnlI CCTC 9 cut(s) 120, 143, 258, 285, 411, 417, 418, 559, 575
MseI TTAA 1 cut(s) 671
Mva1269I GAATGC 1 cut(s) 42
MwoI GCNNNNNNNGC 1 cut(s) 404
NdeI CATATG 2 cut(s) 172, 337
NdeII GATC 2 cut(s) 146, 498
NlaIII CATG 3 cut(s) 407, 596, 643
NlaIV GGNNCC 3 cut(s) 99, 459, 471
PctI GAATGC 1 cut(s) 42
PkrI GCNGC 1 cut(s) 526
PleI GAGTC 3 cut(s) 545, 623, 652
PpsI GAGTC 3 cut(s) 545, 623, 652
PpuMI RGGWCCY 1 cut(s) 98
PshAI GACNNNNGTC 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 98
PspN4I GGNNCC 3 cut(s) 99, 459, 471
PspPI GGNCC 1 cut(s) 98
PspPPI RGGWCCY 1 cut(s) 98
RsaI GTAC 1 cut(s) 465
RsaNI GTAC 1 cut(s) 464
SaqAI TTAA 1 cut(s) 671
SatI GCNGC 1 cut(s) 525
Sau3AI GATC 2 cut(s) 146, 498
Sau96I GGNCC 1 cut(s) 98
ScaI AGTACT 1 cut(s) 465
SchI GAGTC 3 cut(s) 546, 623, 653
SduI GDGCHC 1 cut(s) 460
SetI ASST 7 cut(s) 28, 60, 103, 250, 449, 519, 590
SfaNI GCATC 1 cut(s) 511
SfcI CTRYAG 1 cut(s) 451
SinI GGWCC 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 2 cut(s) 285, 326
SspI AATATT 1 cut(s) 51
SspMI CTAG 2 cut(s) 23, 261
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 2 cut(s) 519, 590
TaqI TCGA 2 cut(s) 207, 564
TasI AATT 2 cut(s) 285, 326
TatI WGTACW 1 cut(s) 463
Tru1I TTAA 1 cut(s) 671
Tru9I TTAA 1 cut(s) 671
TscAI CASTG 1 cut(s) 286
TseI GCWGC 1 cut(s) 524
TspDTI ATGAA 4 cut(s) 193, 206, 532, 638
TspRI CASTG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 98
XagI CCTNNNNNAGG 1 cut(s) 451
XapI RAATTY 1 cut(s) 326
XmiI GTMKAC 2 cut(s) 231, 429
XspI CTAG 2 cut(s) 23, 261
ZrmI AGTACT 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.