Prupe.8G033100_v2.0.a1

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000532
Physical Location & Seq
Forward (+)
3095534 .. 3096653
1120 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G033100.1

Sequence Viewer

Length: 738 bp
ATGCCGCTGTTGACCGCATCTCATATCTACACTACAGATATATTGCGCTTCATGCGCATATATGCATATACATCCTACAGAATGAAAATGACTTCTTGCTTAAAGAAGGTGGTGTTTTTGCTGCTCTTGAGCTCTTTGGCAGCCCAGCAGCATTTATGTGCTGCTGATCCCACTGATGGCTTCACCCGTGTGCAATTCACGGACGAAAACCGCATGTTACAGAAGCCATATGACAAAGCTCCTGAGGATCGTTACAGTAAGGTGGATGGAGTTGAAAAGTTCTGGATCTACACCAATGACAAGCCCTTTAAAGAAGGCAGTCCAACCAGACCACGCACGGAAATAAGAATCACTGGACATGACTACACTTCTGGAGTTTGGCAATTTGAAGGCAACTTTTTTGTGCCACAAGGCACCTCGGGGGCTATAATAATGCAGGTCTTTGGTGCAGCCAATGAAGCTACAACTCTGCAACTAAGGGTCGTTAATGGGGATTTGAAGTACTATGGGAACAGTGTGGTGGCTGCTAATATCTACAGTAGATGGGTTAGACTGAATGTGATTCACAATGTTGGTGCAGGGAAAGTGACAATTTTTATCGACGGCGACCAGAAGCTAGTCGTCAACGATCACGGTCGAGCTACTTTTTACTTCAAATATGGAGTTTATGGTGCCTTATCTGGTTCAAGTAACTACATGGAATCAAGATGGAAGGGAATCAAACTTTTCAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

246

Amino Acids

27.9

Weight (kDa)

9.48

Isoelectric Point (pI)

29.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 56
Acc36I ACCTGC 1 cut(s) 427
AccB1I GGYRCC 2 cut(s) 413, 671
AciI CCGC 3 cut(s) 5, 15, 211
AclWI GGATC 3 cut(s) 161, 255, 293
AfaI GTAC 1 cut(s) 503
AfiI CCNNNNNNNGG 1 cut(s) 176
AgsI TTSAA 6 cut(s) 275, 389, 499, 655, 687, 730
AleI CACNNNNGTG 1 cut(s) 188
AluBI AGCT 5 cut(s) 132, 239, 461, 616, 641
AluI AGCT 5 cut(s) 132, 239, 461, 616, 641
Alw21I GWGCWC 1 cut(s) 134
AlwI GGATC 3 cut(s) 161, 255, 293
Ama87I CYCGRG 1 cut(s) 418
ApeKI GCWGC 6 cut(s) 121, 140, 148, 161, 449, 524
AspLEI GCGC 2 cut(s) 48, 57
AsuHPI GGTGA 1 cut(s) 175
AvaI CYCGRG 1 cut(s) 418
AxyI CCTNAGG 1 cut(s) 243
BanI GGYRCC 2 cut(s) 413, 671
BanII GRGCYC 1 cut(s) 134
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 6 cut(s) 108, 148, 152, 160, 461, 511
BccI CCATC 4 cut(s) 170, 260, 537, 702
BceAI ACGGC 1 cut(s) 619
BfaI CTAG 1 cut(s) 617
BfmI CTRYAG 3 cut(s) 33, 76, 535
BfuAI ACCTGC 1 cut(s) 427
BisI GCNGC 7 cut(s) 5, 122, 141, 149, 162, 450, 525
BlsI GCNGC 7 cut(s) 6, 123, 142, 150, 163, 451, 526
BmcAI AGTACT 1 cut(s) 503
BmeT110I CYCGRG 1 cut(s) 418
BmiI GGNNCC 2 cut(s) 415, 673
BmsI GCATC 1 cut(s) 26
BpmI CTGGAG 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 148
BsaJI CCNNGG 1 cut(s) 417
Bsc4I CCNNNNNNNGG 1 cut(s) 176
Bse1I ACTGG 1 cut(s) 358
Bse21I CCTNAGG 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 417
BseGI GGATG 2 cut(s) 71, 271
BseLI CCNNNNNNNGG 1 cut(s) 176
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 358
BseXI GCAGC 6 cut(s) 108, 148, 152, 160, 461, 511
BseYI CCCAGC 1 cut(s) 144
BsgI GTGCAG 2 cut(s) 468, 597
Bsh1285I CGRYCG 1 cut(s) 637
BshNI GGYRCC 2 cut(s) 413, 671
BsiEI CGRYCG 1 cut(s) 637
BsiHKAI GWGCWC 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 418
BslI CCNNNNNNNGG 1 cut(s) 176
BsoBI CYCGRG 1 cut(s) 418
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 4 cut(s) 166, 247, 285, 628
BspACI CCGC 3 cut(s) 5, 15, 211
BspCNI CTCAG 1 cut(s) 235
BspLI GGNNCC 2 cut(s) 415, 673
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 3 cut(s) 161, 255, 293
BspT107I GGYRCC 2 cut(s) 413, 671
BsrI ACTGG 1 cut(s) 358
BssECI CCNNGG 1 cut(s) 417
BssMI GATC 4 cut(s) 166, 247, 285, 628
Bst4CI ACNGT 4 cut(s) 257, 515, 539, 635
BstDEI CTNAG 2 cut(s) 243, 476
BstF5I GGATG 2 cut(s) 71, 271
BstHHI GCGC 2 cut(s) 48, 57
BstKTI GATC 4 cut(s) 169, 250, 288, 631
BstMBI GATC 4 cut(s) 166, 247, 285, 628
BstMCI CGRYCG 1 cut(s) 637
BstMWI GCNNNNNNNGC 3 cut(s) 52, 54, 458
BstNSI RCATGY 1 cut(s) 217
BstSFI CTRYAG 3 cut(s) 33, 76, 535
BstV1I GCAGC 6 cut(s) 108, 148, 152, 160, 461, 511
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
Bsu36I CCTNAGG 1 cut(s) 243
BtsCI GGATG 2 cut(s) 71, 271
BtsIMutI CAGTG 3 cut(s) 171, 351, 520
BveI ACCTGC 1 cut(s) 427
CfoI GCGC 2 cut(s) 48, 57
Csp6I GTAC 1 cut(s) 502
CviAII CATG 4 cut(s) 52, 214, 359, 697
CviQI GTAC 1 cut(s) 502
DdeI CTNAG 2 cut(s) 243, 476
DpnI GATC 4 cut(s) 168, 249, 287, 630
DpnII GATC 4 cut(s) 166, 247, 285, 628
DraI TTTAAA 1 cut(s) 310
Ecl136II GAGCTC 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 134
Eco53kI GAGCTC 1 cut(s) 132
Eco81I CCTNAGG 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 418
EcoICRI GAGCTC 1 cut(s) 132
EcoT22I ATGCAT 1 cut(s) 67
EcoT38I GRGCYC 1 cut(s) 134
FaeI CATG 4 cut(s) 55, 217, 362, 700
FatI CATG 4 cut(s) 51, 213, 358, 696
FauNDI CATATG 1 cut(s) 229
Fnu4HI GCNGC 7 cut(s) 5, 122, 141, 149, 162, 450, 525
FokI GGATG 2 cut(s) 58, 278
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 7 cut(s) 5, 122, 141, 149, 162, 450, 525
FspAI RTGCGCAY 1 cut(s) 56
FspBI CTAG 1 cut(s) 617
FspI TGCGCA 1 cut(s) 56
GlaI GCGC 2 cut(s) 47, 56
GluI GCNGC 7 cut(s) 5, 122, 141, 149, 162, 450, 525
GsaI CCCAGC 1 cut(s) 148
GsuI CTGGAG 1 cut(s) 393
HhaI GCGC 2 cut(s) 48, 57
Hin1II CATG 4 cut(s) 55, 217, 362, 700
Hin6I GCGC 2 cut(s) 46, 55
HinP1I GCGC 2 cut(s) 46, 55
HincII GTYRAC 2 cut(s) 12, 625
HindII GTYRAC 2 cut(s) 12, 625
HinfI GANTC 4 cut(s) 348, 562, 701, 717
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 2 cut(s) 12, 625
Hpy188III TCNNGA 5 cut(s) 127, 242, 283, 372, 705
Hpy8I GTNNAC 2 cut(s) 12, 625
Hpy99I CGWCG 1 cut(s) 605
HpyAV CCTTC 4 cut(s) 100, 308, 383, 706
HpyCH4III ACNGT 4 cut(s) 257, 515, 539, 635
HpyCH4V TGCA 6 cut(s) 65, 193, 436, 449, 472, 578
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 54, 458
HpyF3I CTNAG 2 cut(s) 243, 476
Hsp92II CATG 4 cut(s) 55, 217, 362, 700
HspAI GCGC 2 cut(s) 46, 55
Kzo9I GATC 4 cut(s) 166, 247, 285, 628
LmnI GCTCC 1 cut(s) 244
Lsp1109I GCAGC 6 cut(s) 108, 148, 152, 160, 461, 511
LweI GCATC 1 cut(s) 26
MaeI CTAG 1 cut(s) 617
MaeIII GTNAC 4 cut(s) 216, 251, 586, 689
MalI GATC 4 cut(s) 168, 249, 287, 630
MboI GATC 4 cut(s) 166, 247, 285, 628
MflI RGATCY 1 cut(s) 285
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 3 cut(s) 194, 383, 591
MmeI TCCRAC 1 cut(s) 347
MnlI CCTC 2 cut(s) 238, 427
Mph1103I ATGCAT 1 cut(s) 67
MseI TTAA 3 cut(s) 101, 309, 486
MslI CAYNNNNRTG 2 cut(s) 156, 188
MspA1I CMGCKG 1 cut(s) 7
MwoI GCNNNNNNNGC 3 cut(s) 52, 54, 458
NdeI CATATG 1 cut(s) 229
NdeII GATC 4 cut(s) 166, 247, 285, 628
NlaIII CATG 4 cut(s) 55, 217, 362, 700
NlaIV GGNNCC 2 cut(s) 415, 673
NmuCI GTSAC 1 cut(s) 586
NsbI TGCGCA 1 cut(s) 56
NsiI ATGCAT 1 cut(s) 67
NspI RCATGY 1 cut(s) 217
OliI CACNNNNGTG 1 cut(s) 188
PfeI GAWTC 4 cut(s) 348, 562, 701, 717
PkrI GCNGC 7 cut(s) 6, 123, 142, 150, 163, 451, 526
Psp124BI GAGCTC 1 cut(s) 134
PspFI CCCAGC 1 cut(s) 144
PspN4I GGNNCC 2 cut(s) 415, 673
PsuI RGATCY 1 cut(s) 285
RsaI GTAC 1 cut(s) 503
RsaNI GTAC 1 cut(s) 502
RseI CAYNNNNRTG 2 cut(s) 156, 188
SacI GAGCTC 1 cut(s) 134
SaqAI TTAA 3 cut(s) 101, 309, 486
SatI GCNGC 7 cut(s) 5, 122, 141, 149, 162, 450, 525
Sau3AI GATC 4 cut(s) 166, 247, 285, 628
ScaI AGTACT 1 cut(s) 503
SduI GDGCHC 1 cut(s) 134
SetI ASST 9 cut(s) 111, 134, 241, 264, 419, 441, 463, 618, 643
SfaNI GCATC 1 cut(s) 26
SfcI CTRYAG 3 cut(s) 33, 76, 535
SmiMI CAYNNNNRTG 2 cut(s) 156, 188
SmlI CTYRAG 1 cut(s) 127
SmoI CTYRAG 1 cut(s) 127
Sse9I AATT 3 cut(s) 194, 383, 591
SsiI CCGC 3 cut(s) 5, 15, 211
SspMI CTAG 1 cut(s) 617
SstI GAGCTC 1 cut(s) 134
TaaI ACNGT 4 cut(s) 257, 515, 539, 635
TaqI TCGA 2 cut(s) 600, 637
TasI AATT 3 cut(s) 194, 383, 591
TatI WGTACW 1 cut(s) 501
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 4 cut(s) 348, 562, 701, 717
Tru1I TTAA 3 cut(s) 101, 309, 486
Tru9I TTAA 3 cut(s) 101, 309, 486
TscAI CASTG 3 cut(s) 178, 358, 520
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 6 cut(s) 121, 140, 148, 161, 449, 524
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 3 cut(s) 40, 98, 471
TspGWI ACGGA 2 cut(s) 215, 353
TspRI CASTG 3 cut(s) 178, 358, 520
XceI RCATGY 1 cut(s) 217
XspI CTAG 1 cut(s) 617
ZrmI AGTACT 1 cut(s) 503
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.