FvH4_2g13550

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000532
Physical Location & Seq
Forward (+)
11838758 .. 11839948
1191 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13550.t1

Sequence Viewer

Length: 567 bp
ATGCTAGGTCTGGATTTGATGATCCGACAAGCAAAAGAACAACAACCAATATATGAAAGCAACTATCATGTTGCTGCTACAAATAGGGCTGCTCATTTTCTGGATATACAACAACGACAAGCCCTTCAAGACAGACAGCACACAACTAGGCCACGCTCCGAACTACGCATAAAGGGACATGATTATTCGTCTGGAATCTGGCAATTTGAAGGCTATGCATATGTTCCAAGTGGTACTTCTGGGGTTACAATAGTGCAGATTCATGGGGCAACTGAAGGAGCTACAACTCTACAACTGAGGATGTATGAAGGAGACGGTGGAGATCTCAGATACTACAGATACAACCTAGTTGATACAGGTCTCTACGATAAGTGGTTCAGAGTAAACATAATCCACAATGTTGGCAAGGGGAAGATCATAGTTTTCATTGACGGTGTTAAAAAGTTTGTGGTCAAAGATCAGGGACCAGGAGACCTCTACTTCAAATGTGGTGTGTATGCTGCACCATTTAATTCCAGTAACTACATGGAATCAAGGTGGAAGGAGATCAAACTTTATGAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

189

Amino Acids

21.64

Weight (kDa)

9.02

Isoelectric Point (pI)

39.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 21 - 186 9.1e-24 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 16
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 1 cut(s) 235
AgsI TTSAA 3 cut(s) 128, 209, 484
AjnI CCWGG 1 cut(s) 466
AjuI GAANNNNNNNTTGG 2 cut(s) 220, 252
AluBI AGCT 1 cut(s) 281
AluI AGCT 1 cut(s) 281
Alw26I GTCTC 3 cut(s) 306, 365, 465
AlwI GGATC 1 cut(s) 16
AoxI GGCC 1 cut(s) 149
ApeKI GCWGC 3 cut(s) 74, 89, 500
AspS9I GGNCC 1 cut(s) 464
AvaII GGWCC 1 cut(s) 464
BbvI GCAGC 3 cut(s) 61, 76, 487
BciT130I CCWGG 1 cut(s) 468
BcoDI GTCTC 3 cut(s) 306, 365, 465
BfaI CTAG 3 cut(s) 5, 147, 347
BfmI CTRYAG 1 cut(s) 334
BglII AGATCT 1 cut(s) 322
BisI GCNGC 3 cut(s) 75, 90, 501
BlsI GCNGC 3 cut(s) 76, 91, 502
Bme1390I CCNGG 1 cut(s) 468
Bme18I GGWCC 1 cut(s) 464
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 1 cut(s) 468
BsaI GGTCTC 2 cut(s) 365, 465
Bse1I ACTGG 1 cut(s) 516
BseBI CCWGG 1 cut(s) 468
BseGI GGATG 1 cut(s) 306
BseMII CTCAG 2 cut(s) 287, 340
BseNI ACTGG 1 cut(s) 516
BseXI GCAGC 3 cut(s) 61, 76, 487
BsgI GTGCAG 2 cut(s) 275, 486
BshFI GGCC 1 cut(s) 151
BslFI GGGAC 2 cut(s) 189, 477
BsmAI GTCTC 3 cut(s) 306, 365, 465
BsmBI CGTCTC 1 cut(s) 306
BsmFI GGGAC 2 cut(s) 189, 477
BsnI GGCC 1 cut(s) 151
Bso31I GGTCTC 2 cut(s) 365, 465
Bsp143I GATC 5 cut(s) 21, 322, 414, 457, 546
BspANI GGCC 1 cut(s) 151
BspCNI CTCAG 2 cut(s) 288, 339
BspLI GGNNCC 1 cut(s) 465
BspPI GGATC 1 cut(s) 16
BspTNI GGTCTC 2 cut(s) 365, 465
BsrI ACTGG 1 cut(s) 516
BssMI GATC 5 cut(s) 21, 322, 414, 457, 546
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 2 cut(s) 317, 434
BstDEI CTNAG 2 cut(s) 296, 326
BstF5I GGATG 1 cut(s) 306
BstKTI GATC 5 cut(s) 24, 325, 417, 460, 549
BstMAI GTCTC 3 cut(s) 306, 365, 465
BstMBI GATC 5 cut(s) 21, 322, 414, 457, 546
BstNI CCWGG 1 cut(s) 468
BstSCI CCNGG 1 cut(s) 466
BstSFI CTRYAG 1 cut(s) 334
BstV1I GCAGC 3 cut(s) 61, 76, 487
BstX2I RGATCY 1 cut(s) 322
BstXI CCANNNNNNTGG 1 cut(s) 401
BstYI RGATCY 1 cut(s) 322
BsuRI GGCC 1 cut(s) 151
BtsCI GGATG 1 cut(s) 306
Cfr13I GGNCC 1 cut(s) 464
Csp6I GTAC 1 cut(s) 234
CviAII CATG 4 cut(s) 68, 179, 263, 526
CviJI RGCY 5 cut(s) 89, 122, 151, 213, 281
CviKI_1 RGCY 5 cut(s) 89, 122, 151, 213, 281
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 2 cut(s) 296, 326
DpnI GATC 5 cut(s) 23, 324, 416, 459, 548
DpnII GATC 5 cut(s) 21, 322, 414, 457, 546
Eco31I GGTCTC 2 cut(s) 365, 465
Eco47I GGWCC 1 cut(s) 464
Eco57I CTGAAG 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 466
EcoT22I ATGCAT 1 cut(s) 220
Esp3I CGTCTC 1 cut(s) 306
FaeI CATG 4 cut(s) 71, 182, 266, 529
FalI AAGNNNNNCTT 2 cut(s) 220, 252
FaqI GGGAC 2 cut(s) 189, 477
FatI CATG 4 cut(s) 67, 178, 262, 525
FauNDI CATATG 1 cut(s) 220
Fnu4HI GCNGC 3 cut(s) 75, 90, 501
FokI GGATG 1 cut(s) 313
Fsp4HI GCNGC 3 cut(s) 75, 90, 501
FspBI CTAG 3 cut(s) 5, 147, 347
GluI GCNGC 3 cut(s) 75, 90, 501
HaeIII GGCC 1 cut(s) 151
Hin1II CATG 4 cut(s) 71, 182, 266, 529
HinfI GANTC 3 cut(s) 195, 259, 530
Hpy166II GTNNAC 1 cut(s) 385
Hpy188I TCNGA 4 cut(s) 26, 160, 329, 380
Hpy188III TCNNGA 4 cut(s) 11, 101, 128, 192
Hpy8I GTNNAC 1 cut(s) 385
HpyAV CCTTC 5 cut(s) 134, 203, 269, 302, 535
HpyCH4III ACNGT 2 cut(s) 317, 434
HpyCH4V TGCA 3 cut(s) 218, 256, 503
HpyF3I CTNAG 2 cut(s) 296, 326
Hsp92II CATG 4 cut(s) 71, 182, 266, 529
Kzo9I GATC 5 cut(s) 21, 322, 414, 457, 546
LmnI GCTCC 2 cut(s) 161, 278
LpnPI CCDG 9 cut(s) 86, 177, 184, 225, 342, 446, 453, 480, 529
Lsp1109I GCAGC 3 cut(s) 61, 76, 487
MaeI CTAG 3 cut(s) 5, 147, 347
MaeIII GTNAC 2 cut(s) 244, 518
MalI GATC 5 cut(s) 23, 324, 416, 459, 548
MboI GATC 5 cut(s) 21, 322, 414, 457, 546
MboII GAAGA 1 cut(s) 424
MflI RGATCY 1 cut(s) 322
MluCI AATT 2 cut(s) 203, 511
MmeI TCCRAC 1 cut(s) 49
MnlI CCTC 2 cut(s) 291, 485
Mph1103I ATGCAT 1 cut(s) 220
MseI TTAA 2 cut(s) 438, 510
MspR9I CCNGG 1 cut(s) 468
MvaI CCWGG 1 cut(s) 468
NdeI CATATG 1 cut(s) 220
NdeII GATC 5 cut(s) 21, 322, 414, 457, 546
NlaIII CATG 4 cut(s) 71, 182, 266, 529
NlaIV GGNNCC 1 cut(s) 465
NsiI ATGCAT 1 cut(s) 220
PfeI GAWTC 3 cut(s) 195, 259, 530
PkrI GCNGC 3 cut(s) 76, 91, 502
Psp6I CCWGG 1 cut(s) 466
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 1 cut(s) 464
PsuI RGATCY 1 cut(s) 322
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SaqAI TTAA 2 cut(s) 438, 510
SatI GCNGC 3 cut(s) 75, 90, 501
Sau3AI GATC 5 cut(s) 21, 322, 414, 457, 546
Sau96I GGNCC 1 cut(s) 464
ScrFI CCNGG 1 cut(s) 468
SetI ASST 6 cut(s) 10, 283, 348, 361, 477, 539
SfcI CTRYAG 1 cut(s) 334
SinI GGWCC 1 cut(s) 464
Sse9I AATT 2 cut(s) 203, 511
SspMI CTAG 3 cut(s) 5, 147, 347
StyD4I CCNGG 1 cut(s) 466
TaaI ACNGT 2 cut(s) 317, 434
TasI AATT 2 cut(s) 203, 511
TfiI GAWTC 3 cut(s) 195, 259, 530
Tru1I TTAA 2 cut(s) 438, 510
Tru9I TTAA 2 cut(s) 438, 510
TseI GCWGC 3 cut(s) 74, 89, 500
TspDTI ATGAA 4 cut(s) 69, 251, 321, 415
VpaK11BI GGWCC 1 cut(s) 464
XcmI CCANNNNNNNNNTGG 1 cut(s) 523
XspI CTAG 3 cut(s) 5, 147, 347
Zsp2I ATGCAT 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.