Rh6AG202900

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000532
Physical Location & Seq
Forward (+)
36314404 .. 36316186
1783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG202900.1

Sequence Viewer

Length: 663 bp
ATGAAAATGAATTCCTTCTACTACAAGTTGTTGTGTTTGCTCATTCTGAGCTTCTTCGAAGGCCACTATCTTTGTGGTGCTGATCCTACTGATGGCTTCACCCTTGTGCCATTAACAGAAGACAACTTCAAGCTGCAAAAGCCATACAATGAACCCCTTGATGATCGTTACAGTTACAAAGATGGAGTTCGAAGTTTCTGGATCTACAACAATGACAAGCCCTACAGCCCAGATAGCCCAACCAGACCACGCTCAGAACTGCGCATATCGGGACATGACTATTCGTCTGGAATCTGGCAATTTGAAGGCTATGCATATGTGCCCAGTGGTACGTCTGGGGTTACAATAGTGCAGATTCATGGTGCAAGTGAGGGAGAGACAACTCTCCAACTAAGGATGTATGAAGGAGATGGTGGAGATCTCAGATACTACAGATACAACTTAGTTGATACTGGTCTCTATGATAAGTGGTTCAGAGTAAACATAATCCACAATGTTGACAAAGGGAAGGTCATAGTTTTCATTGACGGTGTTAAGAAGTTTGTGGTGAAAGATCAGGGACCAGGGGACCTGTACTTCAAATGTGGTGTGTATGCAGCACCGTTTAATTCCAGTAACTACATGGAATCAAGGTGGAAGGAAATCAACATTTACAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

220

Amino Acids

25.47

Weight (kDa)

6.43

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 41 - 218 4.8e-27 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 263
AclWI GGATC 2 cut(s) 77, 209
AcsI RAATTY 1 cut(s) 10
AfaI GTAC 2 cut(s) 331, 575
AfiI CCNNNNNNNGG 1 cut(s) 92
AgsI TTSAA 3 cut(s) 130, 305, 580
AhdI GACNNNNNGTC 1 cut(s) 283
AjnI CCWGG 1 cut(s) 562
AleI CACNNNNGTG 1 cut(s) 104
AluBI AGCT 2 cut(s) 51, 133
AluI AGCT 2 cut(s) 51, 133
Alw26I GTCTC 2 cut(s) 371, 461
AlwI GGATC 2 cut(s) 77, 209
AoxI GGCC 1 cut(s) 61
ApeKI GCWGC 2 cut(s) 133, 596
ApoI RAATTY 1 cut(s) 10
ArsI GACNNNNNNTTYG 2 cut(s) 495, 527
Asp700I GAANNNNTTC 1 cut(s) 14
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 2 cut(s) 560, 568
AsuHPI GGTGA 2 cut(s) 91, 559
AsuII TTCGAA 2 cut(s) 57, 190
AvaII GGWCC 2 cut(s) 560, 568
BaeGI GKGCMC 1 cut(s) 324
BbsI GAAGAC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 120, 608
BccI CCATC 3 cut(s) 86, 176, 404
BciT130I CCWGG 1 cut(s) 564
BcoDI GTCTC 2 cut(s) 371, 461
BfmI CTRYAG 2 cut(s) 223, 430
BglII AGATCT 1 cut(s) 418
BisI GCNGC 2 cut(s) 134, 597
BlsI GCNGC 2 cut(s) 135, 598
Bme1390I CCNGG 1 cut(s) 564
Bme18I GGWCC 2 cut(s) 560, 568
BmeRI GACNNNNNGTC 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 560, 568
BmiI GGNNCC 2 cut(s) 561, 569
BmrFI CCNGG 1 cut(s) 564
BmrI ACTGGG 1 cut(s) 318
BmuI ACTGGG 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 126
Bpu14I TTCGAA 2 cut(s) 57, 190
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 1 cut(s) 563
Bsc4I CCNNNNNNNGG 1 cut(s) 92
Bse1I ACTGG 3 cut(s) 324, 457, 612
BseBI CCWGG 1 cut(s) 564
BseDI CCNNGG 1 cut(s) 563
BseGI GGATG 1 cut(s) 402
BseLI CCNNNNNNNGG 1 cut(s) 92
BseMII CTCAG 3 cut(s) 38, 267, 436
BseNI ACTGG 3 cut(s) 324, 457, 612
BseSI GKGCMC 1 cut(s) 324
BseXI GCAGC 2 cut(s) 120, 608
BsgI GTGCAG 1 cut(s) 371
BshFI GGCC 1 cut(s) 63
BslFI GGGAC 3 cut(s) 285, 573, 581
BslI CCNNNNNNNGG 1 cut(s) 92
BsmAI GTCTC 2 cut(s) 371, 461
BsmFI GGGAC 3 cut(s) 285, 573, 581
BsnI GGCC 1 cut(s) 63
Bso31I GGTCTC 1 cut(s) 461
Bsp119I TTCGAA 2 cut(s) 57, 190
Bsp1286I GDGCHC 1 cut(s) 324
Bsp143I GATC 5 cut(s) 82, 163, 201, 418, 553
BspANI GGCC 1 cut(s) 63
BspCNI CTCAG 3 cut(s) 39, 266, 435
BspLI GGNNCC 2 cut(s) 561, 569
BspPI GGATC 2 cut(s) 77, 209
BspT104I TTCGAA 2 cut(s) 57, 190
BspTNI GGTCTC 1 cut(s) 461
BsrI ACTGG 3 cut(s) 324, 457, 612
BssECI CCNNGG 1 cut(s) 563
BssMI GATC 5 cut(s) 82, 163, 201, 418, 553
Bst2UI CCWGG 1 cut(s) 564
Bst4CI ACNGT 3 cut(s) 173, 530, 603
BstBI TTCGAA 2 cut(s) 57, 190
BstDEI CTNAG 5 cut(s) 47, 253, 392, 422, 442
BstF5I GGATG 1 cut(s) 402
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 5 cut(s) 85, 166, 204, 421, 556
BstMAI GTCTC 2 cut(s) 371, 461
BstMBI GATC 5 cut(s) 82, 163, 201, 418, 553
BstMWI GCNNNNNNNGC 2 cut(s) 139, 234
BstNI CCWGG 1 cut(s) 564
BstSCI CCNGG 1 cut(s) 562
BstSFI CTRYAG 2 cut(s) 223, 430
BstSLI GKGCMC 1 cut(s) 324
BstV1I GCAGC 2 cut(s) 120, 608
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 2 cut(s) 201, 418
BstYI RGATCY 2 cut(s) 201, 418
BsuRI GGCC 1 cut(s) 63
BtsCI GGATG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 331
CfoI GCGC 1 cut(s) 264
Cfr13I GGNCC 2 cut(s) 560, 568
Csp6I GTAC 2 cut(s) 330, 574
CspCI CAANNNNNGTGG 2 cut(s) 53, 88
CviAII CATG 3 cut(s) 275, 359, 622
CviJI RGCY 9 cut(s) 51, 63, 96, 133, 142, 220, 228, 237, 309
CviKI_1 RGCY 9 cut(s) 51, 63, 96, 133, 142, 220, 228, 237, 309
CviQI GTAC 2 cut(s) 330, 574
DdeI CTNAG 5 cut(s) 47, 253, 392, 422, 442
DpnI GATC 5 cut(s) 84, 165, 203, 420, 555
DpnII GATC 5 cut(s) 82, 163, 201, 418, 553
DriI GACNNNNNGTC 1 cut(s) 283
Eam1105I GACNNNNNGTC 1 cut(s) 283
Eco31I GGTCTC 1 cut(s) 461
Eco47I GGWCC 2 cut(s) 560, 568
EcoO109I RGGNCCY 1 cut(s) 568
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 562
EcoT22I ATGCAT 1 cut(s) 316
FaeI CATG 3 cut(s) 278, 362, 625
FaqI GGGAC 3 cut(s) 285, 573, 581
FatI CATG 3 cut(s) 274, 358, 621
FauNDI CATATG 1 cut(s) 316
Fnu4HI GCNGC 2 cut(s) 134, 597
FokI GGATG 1 cut(s) 409
Fsp4HI GCNGC 2 cut(s) 134, 597
FspI TGCGCA 1 cut(s) 263
GlaI GCGC 1 cut(s) 263
GluI GCNGC 2 cut(s) 134, 597
HaeIII GGCC 1 cut(s) 63
HhaI GCGC 1 cut(s) 264
Hin1II CATG 3 cut(s) 278, 362, 625
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HinfI GANTC 3 cut(s) 291, 355, 626
HphI GGTGA 2 cut(s) 91, 559
Hpy166II GTNNAC 2 cut(s) 481, 499
Hpy188I TCNGA 4 cut(s) 48, 256, 425, 476
Hpy188III TCNNGA 3 cut(s) 199, 270, 288
Hpy8I GTNNAC 2 cut(s) 481, 499
HpyAV CCTTC 6 cut(s) 25, 53, 299, 398, 502, 631
HpyCH4III ACNGT 3 cut(s) 173, 530, 603
HpyCH4IV ACGT 1 cut(s) 332
HpyCH4V TGCA 5 cut(s) 136, 314, 352, 365, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 234
HpyF3I CTNAG 5 cut(s) 47, 253, 392, 422, 442
HpySE526I ACGT 1 cut(s) 332
Hsp92II CATG 3 cut(s) 278, 362, 625
HspAI GCGC 1 cut(s) 262
Kzo9I GATC 5 cut(s) 82, 163, 201, 418, 553
Lsp1109I GCAGC 2 cut(s) 120, 608
MaeII ACGT 1 cut(s) 332
MaeIII GTNAC 4 cut(s) 167, 173, 340, 614
MalI GATC 5 cut(s) 84, 165, 203, 420, 555
MboI GATC 5 cut(s) 82, 163, 201, 418, 553
MboII GAAGA 2 cut(s) 46, 131
MflI RGATCY 2 cut(s) 201, 418
MhlI GDGCHC 1 cut(s) 324
MluCI AATT 3 cut(s) 10, 299, 607
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 1 cut(s) 364
Mph1103I ATGCAT 1 cut(s) 316
MroXI GAANNNNTTC 1 cut(s) 14
MseI TTAA 3 cut(s) 113, 534, 606
MslI CAYNNNNRTG 1 cut(s) 104
MspR9I CCNGG 1 cut(s) 564
MvaI CCWGG 1 cut(s) 564
MwoI GCNNNNNNNGC 2 cut(s) 139, 234
NdeI CATATG 1 cut(s) 316
NdeII GATC 5 cut(s) 82, 163, 201, 418, 553
NlaIII CATG 3 cut(s) 278, 362, 625
NlaIV GGNNCC 2 cut(s) 561, 569
NsbI TGCGCA 1 cut(s) 263
NsiI ATGCAT 1 cut(s) 316
NspV TTCGAA 2 cut(s) 57, 190
OliI CACNNNNGTG 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 14
PfeI GAWTC 3 cut(s) 291, 355, 626
PkrI GCNGC 2 cut(s) 135, 598
PpuMI RGGWCCY 1 cut(s) 568
Psp5II RGGWCCY 1 cut(s) 568
Psp6I CCWGG 1 cut(s) 562
PspGI CCWGG 1 cut(s) 562
PspN4I GGNNCC 2 cut(s) 561, 569
PspPI GGNCC 2 cut(s) 560, 568
PspPPI RGGWCCY 1 cut(s) 568
PsuI RGATCY 2 cut(s) 201, 418
RsaI GTAC 2 cut(s) 331, 575
RsaNI GTAC 2 cut(s) 330, 574
RseI CAYNNNNRTG 1 cut(s) 104
SaqAI TTAA 3 cut(s) 113, 534, 606
SatI GCNGC 2 cut(s) 134, 597
Sau3AI GATC 5 cut(s) 82, 163, 201, 418, 553
Sau96I GGNCC 2 cut(s) 560, 568
ScrFI CCNGG 1 cut(s) 564
SduI GDGCHC 1 cut(s) 324
SetI ASST 6 cut(s) 53, 135, 335, 513, 573, 635
SfcI CTRYAG 2 cut(s) 223, 430
SfuI TTCGAA 2 cut(s) 57, 190
SinI GGWCC 2 cut(s) 560, 568
SmiMI CAYNNNNRTG 1 cut(s) 104
Sse9I AATT 3 cut(s) 10, 299, 607
StyD4I CCNGG 1 cut(s) 562
TaaI ACNGT 3 cut(s) 173, 530, 603
TaiI ACGT 1 cut(s) 335
TaqI TCGA 2 cut(s) 57, 190
TasI AATT 3 cut(s) 10, 299, 607
TatI WGTACW 1 cut(s) 573
TfiI GAWTC 3 cut(s) 291, 355, 626
Tru1I TTAA 3 cut(s) 113, 534, 606
Tru9I TTAA 3 cut(s) 113, 534, 606
TscAI CASTG 1 cut(s) 331
TseI GCWGC 2 cut(s) 133, 596
TspDTI ATGAA 6 cut(s) 17, 23, 165, 347, 417, 511
TspRI CASTG 1 cut(s) 331
VpaK11BI GGWCC 2 cut(s) 560, 568
XapI RAATTY 1 cut(s) 10
XcmI CCANNNNNNNNNTGG 2 cut(s) 71, 619
XmnI GAANNNNTTC 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.