Rw6G017610

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000532
Physical Location & Seq
Forward (+)
34916040 .. 34917374
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G017610.1

Sequence Viewer

Length: 657 bp
ATGAATTCCTTCTACTACAAGTTGTTGTGTTTGCTCATTCTGAGCTTCTTCGAAGGCCACTATCTTTGTGGTGCTGATCCTACTGATGGCTTCACCCTTGTGCCATTAACAGAAGACAACTTCAAGCTGCAAAAGCCATACAATGAACCCCTTGATGATCGTTACAGTTACAAAGATGGAGTTCGAAGTTTCTGGATCTACAACAATGACAAGCCCTACAGCCCAGATAGCCCAACCAGACCACGCTCAGAACTGCGCATATCGGGACATGACTATTCGTCTGGAATCTGGCAATTTGAAGGCTATGCATATGTGCCCAGTGGTACGTCTGGGGTTACAATAGTGCAGATTCATGGTGCAAGTGAGGGAGAGACAACTCTCCAACTAAGGATGTATGAAGGAGATGGTGGAGATCTCAGATACTACAGATACAACTTTGTTGATACTGGTCTCTATGATAAGTGGTTCAGAGTAAACATAATCCACAATGTTGACAAAGGGAAGGTCATAGTTTTCATTGACGGTGTTAAGAAGTTTGTGGTGAAAGATCAGGGACCAGGGGACCTGTACTTCAAATGTGGTGTGTATGCAGCACCGTTTAATTCCAGTAACTACATGGAATCAAGGTGGAAGGAAATCAACATTTACAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

218

Amino Acids

25.24

Weight (kDa)

6.09

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 39 - 216 6.7e-27 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 257
AclWI GGATC 2 cut(s) 71, 203
AcsI RAATTY 1 cut(s) 4
AfaI GTAC 2 cut(s) 325, 569
AfiI CCNNNNNNNGG 1 cut(s) 86
AgsI TTSAA 3 cut(s) 124, 299, 574
AhdI GACNNNNNGTC 1 cut(s) 277
AjnI CCWGG 1 cut(s) 556
AleI CACNNNNGTG 1 cut(s) 98
AluBI AGCT 2 cut(s) 45, 127
AluI AGCT 2 cut(s) 45, 127
Alw26I GTCTC 2 cut(s) 365, 455
AlwI GGATC 2 cut(s) 71, 203
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 2 cut(s) 127, 590
ApoI RAATTY 1 cut(s) 4
ArsI GACNNNNNNTTYG 2 cut(s) 489, 521
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 258
AspS9I GGNCC 2 cut(s) 554, 562
AsuHPI GGTGA 2 cut(s) 85, 553
AsuII TTCGAA 2 cut(s) 51, 184
AvaII GGWCC 2 cut(s) 554, 562
BaeGI GKGCMC 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 114, 602
BccI CCATC 3 cut(s) 80, 170, 398
BciT130I CCWGG 1 cut(s) 558
BcoDI GTCTC 2 cut(s) 365, 455
BfmI CTRYAG 2 cut(s) 217, 424
BglII AGATCT 1 cut(s) 412
BisI GCNGC 2 cut(s) 128, 591
BlsI GCNGC 2 cut(s) 129, 592
Bme1390I CCNGG 1 cut(s) 558
Bme18I GGWCC 2 cut(s) 554, 562
BmeRI GACNNNNNGTC 1 cut(s) 277
BmgT120I GGNCC 2 cut(s) 554, 562
BmiI GGNNCC 2 cut(s) 555, 563
BmrFI CCNGG 1 cut(s) 558
BmrI ACTGGG 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 120
Bpu14I TTCGAA 2 cut(s) 51, 184
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 557
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse1I ACTGG 3 cut(s) 318, 451, 606
BseBI CCWGG 1 cut(s) 558
BseDI CCNNGG 1 cut(s) 557
BseGI GGATG 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMII CTCAG 3 cut(s) 32, 261, 430
BseNI ACTGG 3 cut(s) 318, 451, 606
BseSI GKGCMC 1 cut(s) 318
BseXI GCAGC 2 cut(s) 114, 602
BsgI GTGCAG 1 cut(s) 365
BshFI GGCC 1 cut(s) 57
BslFI GGGAC 3 cut(s) 279, 567, 575
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 2 cut(s) 365, 455
BsmFI GGGAC 3 cut(s) 279, 567, 575
BsnI GGCC 1 cut(s) 57
Bso31I GGTCTC 1 cut(s) 455
Bsp119I TTCGAA 2 cut(s) 51, 184
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 5 cut(s) 76, 157, 195, 412, 547
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 3 cut(s) 33, 260, 429
BspLI GGNNCC 2 cut(s) 555, 563
BspPI GGATC 2 cut(s) 71, 203
BspT104I TTCGAA 2 cut(s) 51, 184
BspTNI GGTCTC 1 cut(s) 455
BsrI ACTGG 3 cut(s) 318, 451, 606
BssECI CCNNGG 1 cut(s) 557
BssMI GATC 5 cut(s) 76, 157, 195, 412, 547
Bst2UI CCWGG 1 cut(s) 558
Bst4CI ACNGT 3 cut(s) 167, 524, 597
BstBI TTCGAA 2 cut(s) 51, 184
BstDEI CTNAG 4 cut(s) 41, 247, 386, 416
BstF5I GGATG 1 cut(s) 396
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 5 cut(s) 79, 160, 198, 415, 550
BstMAI GTCTC 2 cut(s) 365, 455
BstMBI GATC 5 cut(s) 76, 157, 195, 412, 547
BstMWI GCNNNNNNNGC 2 cut(s) 133, 228
BstNI CCWGG 1 cut(s) 558
BstSCI CCNGG 1 cut(s) 556
BstSFI CTRYAG 2 cut(s) 217, 424
BstSLI GKGCMC 1 cut(s) 318
BstV1I GCAGC 2 cut(s) 114, 602
BstV2I GAAGAC 1 cut(s) 120
BstX2I RGATCY 2 cut(s) 195, 412
BstYI RGATCY 2 cut(s) 195, 412
BsuRI GGCC 1 cut(s) 57
BtsCI GGATG 1 cut(s) 396
BtsIMutI CAGTG 1 cut(s) 325
CfoI GCGC 1 cut(s) 258
Cfr13I GGNCC 2 cut(s) 554, 562
Csp6I GTAC 2 cut(s) 324, 568
CspCI CAANNNNNGTGG 2 cut(s) 47, 82
CviAII CATG 3 cut(s) 269, 353, 616
CviJI RGCY 9 cut(s) 45, 57, 90, 127, 136, 214, 222, 231, 303
CviKI_1 RGCY 9 cut(s) 45, 57, 90, 127, 136, 214, 222, 231, 303
CviQI GTAC 2 cut(s) 324, 568
DdeI CTNAG 4 cut(s) 41, 247, 386, 416
DpnI GATC 5 cut(s) 78, 159, 197, 414, 549
DpnII GATC 5 cut(s) 76, 157, 195, 412, 547
DriI GACNNNNNGTC 1 cut(s) 277
Eam1105I GACNNNNNGTC 1 cut(s) 277
Eco31I GGTCTC 1 cut(s) 455
Eco47I GGWCC 2 cut(s) 554, 562
EcoO109I RGGNCCY 1 cut(s) 562
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 556
EcoT22I ATGCAT 1 cut(s) 310
FaeI CATG 3 cut(s) 272, 356, 619
FaqI GGGAC 3 cut(s) 279, 567, 575
FatI CATG 3 cut(s) 268, 352, 615
FauNDI CATATG 1 cut(s) 310
Fnu4HI GCNGC 2 cut(s) 128, 591
FokI GGATG 1 cut(s) 403
Fsp4HI GCNGC 2 cut(s) 128, 591
FspI TGCGCA 1 cut(s) 257
GlaI GCGC 1 cut(s) 257
GluI GCNGC 2 cut(s) 128, 591
HaeIII GGCC 1 cut(s) 57
HhaI GCGC 1 cut(s) 258
Hin1II CATG 3 cut(s) 272, 356, 619
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HinfI GANTC 3 cut(s) 285, 349, 620
HphI GGTGA 2 cut(s) 85, 553
Hpy166II GTNNAC 2 cut(s) 475, 493
Hpy188I TCNGA 4 cut(s) 42, 250, 419, 470
Hpy188III TCNNGA 3 cut(s) 193, 264, 282
Hpy8I GTNNAC 2 cut(s) 475, 493
HpyAV CCTTC 6 cut(s) 19, 47, 293, 392, 496, 625
HpyCH4III ACNGT 3 cut(s) 167, 524, 597
HpyCH4IV ACGT 1 cut(s) 326
HpyCH4V TGCA 5 cut(s) 130, 308, 346, 359, 590
HpyF10VI GCNNNNNNNGC 2 cut(s) 133, 228
HpyF3I CTNAG 4 cut(s) 41, 247, 386, 416
HpySE526I ACGT 1 cut(s) 326
Hsp92II CATG 3 cut(s) 272, 356, 619
HspAI GCGC 1 cut(s) 256
Kzo9I GATC 5 cut(s) 76, 157, 195, 412, 547
Lsp1109I GCAGC 2 cut(s) 114, 602
MaeII ACGT 1 cut(s) 326
MaeIII GTNAC 4 cut(s) 161, 167, 334, 608
MalI GATC 5 cut(s) 78, 159, 197, 414, 549
MboI GATC 5 cut(s) 76, 157, 195, 412, 547
MboII GAAGA 2 cut(s) 40, 125
MflI RGATCY 2 cut(s) 195, 412
MhlI GDGCHC 1 cut(s) 318
MluCI AATT 3 cut(s) 4, 293, 601
MmeI TCCRAC 1 cut(s) 406
MnlI CCTC 1 cut(s) 358
Mph1103I ATGCAT 1 cut(s) 310
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 3 cut(s) 107, 528, 600
MslI CAYNNNNRTG 1 cut(s) 98
MspR9I CCNGG 1 cut(s) 558
MvaI CCWGG 1 cut(s) 558
MwoI GCNNNNNNNGC 2 cut(s) 133, 228
NdeI CATATG 1 cut(s) 310
NdeII GATC 5 cut(s) 76, 157, 195, 412, 547
NlaIII CATG 3 cut(s) 272, 356, 619
NlaIV GGNNCC 2 cut(s) 555, 563
NsbI TGCGCA 1 cut(s) 257
NsiI ATGCAT 1 cut(s) 310
NspV TTCGAA 2 cut(s) 51, 184
OliI CACNNNNGTG 1 cut(s) 98
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 3 cut(s) 285, 349, 620
PkrI GCNGC 2 cut(s) 129, 592
PpuMI RGGWCCY 1 cut(s) 562
Psp5II RGGWCCY 1 cut(s) 562
Psp6I CCWGG 1 cut(s) 556
PspGI CCWGG 1 cut(s) 556
PspN4I GGNNCC 2 cut(s) 555, 563
PspPI GGNCC 2 cut(s) 554, 562
PspPPI RGGWCCY 1 cut(s) 562
PsuI RGATCY 2 cut(s) 195, 412
RsaI GTAC 2 cut(s) 325, 569
RsaNI GTAC 2 cut(s) 324, 568
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 3 cut(s) 107, 528, 600
SatI GCNGC 2 cut(s) 128, 591
Sau3AI GATC 5 cut(s) 76, 157, 195, 412, 547
Sau96I GGNCC 2 cut(s) 554, 562
ScrFI CCNGG 1 cut(s) 558
SduI GDGCHC 1 cut(s) 318
SetI ASST 6 cut(s) 47, 129, 329, 507, 567, 629
SfcI CTRYAG 2 cut(s) 217, 424
SfuI TTCGAA 2 cut(s) 51, 184
SinI GGWCC 2 cut(s) 554, 562
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 3 cut(s) 4, 293, 601
StyD4I CCNGG 1 cut(s) 556
TaaI ACNGT 3 cut(s) 167, 524, 597
TaiI ACGT 1 cut(s) 329
TaqI TCGA 2 cut(s) 51, 184
TasI AATT 3 cut(s) 4, 293, 601
TatI WGTACW 1 cut(s) 567
TfiI GAWTC 3 cut(s) 285, 349, 620
Tru1I TTAA 3 cut(s) 107, 528, 600
Tru9I TTAA 3 cut(s) 107, 528, 600
TscAI CASTG 1 cut(s) 325
TseI GCWGC 2 cut(s) 127, 590
TspDTI ATGAA 5 cut(s) 17, 159, 341, 411, 505
TspRI CASTG 1 cut(s) 325
VpaK11BI GGWCC 2 cut(s) 554, 562
XapI RAATTY 1 cut(s) 4
XcmI CCANNNNNNNNNTGG 2 cut(s) 65, 613
XmnI GAANNNNTTC 1 cut(s) 8
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.