RchiOBHm_Chr2g0119711

Alginate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000532
Physical Location & Seq
Forward (+)
32269251 .. 32269655
405 bp
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UTR
Exon/CDS
Intron
PRQ49240

Sequence Viewer

Length: 405 bp
ATGCAAGTGTTTGGAGCAATTGCTCTTCATGCCTCAACTCTGATGTTGAGGGTCTACAATGGTTCACTCACCTACTACAGGGCTCCAGTACTGGTTACAAACATCTACGATAAGTGGTTCAAGATCAATGTGGTTCACGACGTTGATGCTGCGAAAGTCAGAGTCTACATTGACGGCTGTCTCAAAATCGAGGCAAATGGTCGTGGAGGAACGTCTCATGCTTTCAAATGTGGAGTGTATGCCCAGATGAATGACTATTTACATGGAGTCTCGCTGGAAAGACATCAAAGTTTGAAGGAAGTATTGACCTTGATTTTCATTATAAGCTTGTTAGTTAATTTGTTTGTCATAAGAGAAAACCAAAATTGTTCATTGTTAATTTGTTATTGTATGTTTGTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

134

Amino Acids

15.19

Weight (kDa)

8.62

Isoelectric Point (pI)

16.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 1 - 85 5e-08 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 323
AccI GTMKAC 2 cut(s) 54, 165
AfaI GTAC 1 cut(s) 90
AfiI CCNNNNNNNGG 1 cut(s) 78
AgsI TTSAA 3 cut(s) 121, 226, 295
AluBI AGCT 1 cut(s) 327
AluI AGCT 1 cut(s) 327
Alw26I GTCTC 3 cut(s) 185, 219, 274
ApeKI GCWGC 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 61
BanII GRGCYC 1 cut(s) 85
BbvI GCAGC 1 cut(s) 136
BceAI ACGGC 1 cut(s) 190
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BcoDI GTCTC 3 cut(s) 185, 219, 274
BfmI CTRYAG 1 cut(s) 76
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
BmcAI AGTACT 1 cut(s) 90
BmiI GGNNCC 1 cut(s) 84
BmsI GCATC 1 cut(s) 136
BoxI GACNNNNGTC 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 69
Bsc4I CCNNNNNNNGG 1 cut(s) 78
Bse1I ACTGG 2 cut(s) 86, 96
BseLI CCNNNNNNNGG 1 cut(s) 78
BseNI ACTGG 2 cut(s) 86, 96
BseXI GCAGC 1 cut(s) 136
BslI CCNNNNNNNGG 1 cut(s) 78
BsmAI GTCTC 3 cut(s) 185, 219, 274
BsmBI CGTCTC 1 cut(s) 219
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 1 cut(s) 123
BspLI GGNNCC 1 cut(s) 84
BspQI GCTCTTC 1 cut(s) 30
BsrI ACTGG 2 cut(s) 86, 96
BssMI GATC 1 cut(s) 123
Bst6I CTCTTC 1 cut(s) 30
BstENI CCTNNNNNAGG 1 cut(s) 76
BstKTI GATC 1 cut(s) 126
BstMAI GTCTC 3 cut(s) 185, 219, 274
BstMBI GATC 1 cut(s) 123
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstPAI GACNNNNGTC 1 cut(s) 177
BstSFI CTRYAG 1 cut(s) 76
BstV1I GCAGC 1 cut(s) 136
Csp6I GTAC 1 cut(s) 89
CviAII CATG 3 cut(s) 29, 218, 263
CviJI RGCY 3 cut(s) 83, 177, 327
CviKI_1 RGCY 3 cut(s) 83, 177, 327
CviQI GTAC 1 cut(s) 89
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 85
EcoNI CCTNNNNNAGG 1 cut(s) 76
EcoT38I GRGCYC 1 cut(s) 85
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 3 cut(s) 32, 221, 266
FaiI YATR 7 cut(s) 30, 219, 240, 264, 323, 350, 392
FatI CATG 3 cut(s) 28, 217, 262
FblI GTMKAC 2 cut(s) 54, 165
Fnu4HI GCNGC 1 cut(s) 150
FriOI GRGCYC 1 cut(s) 85
Fsp4HI GCNGC 1 cut(s) 150
GluI GCNGC 1 cut(s) 150
GsuI CTGGAG 1 cut(s) 69
Hin1II CATG 3 cut(s) 32, 221, 266
HindIII AAGCTT 1 cut(s) 325
HinfI GANTC 2 cut(s) 162, 267
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 4 cut(s) 55, 65, 136, 166
Hpy188I TCNGA 2 cut(s) 42, 161
Hpy188III TCNNGA 2 cut(s) 121, 137
Hpy8I GTNNAC 4 cut(s) 55, 65, 136, 166
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 1 cut(s) 289
HpyCH4IV ACGT 2 cut(s) 141, 212
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpySE526I ACGT 2 cut(s) 141, 212
Hsp92II CATG 3 cut(s) 32, 221, 266
Kzo9I GATC 1 cut(s) 123
LguI GCTCTTC 1 cut(s) 30
LmnI GCTCC 2 cut(s) 14, 88
LpnPI CCDG 5 cut(s) 64, 77, 99, 257, 260
Lsp1109I GCAGC 1 cut(s) 136
LweI GCATC 1 cut(s) 136
MaeII ACGT 2 cut(s) 141, 212
MaeIII GTNAC 1 cut(s) 94
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MboII GAAGA 1 cut(s) 17
MfeI CAATTG 1 cut(s) 18
MhlI GDGCHC 1 cut(s) 85
MluCI AATT 4 cut(s) 18, 337, 364, 378
MlyI GAGTC 2 cut(s) 171, 276
MnlI CCTC 4 cut(s) 42, 43, 184, 200
MseI TTAA 2 cut(s) 336, 377
MunI CAATTG 1 cut(s) 18
MwoI GCNNNNNNNGC 1 cut(s) 29
NdeII GATC 1 cut(s) 123
NlaIII CATG 3 cut(s) 32, 221, 266
NlaIV GGNNCC 1 cut(s) 84
PciSI GCTCTTC 1 cut(s) 30
PkrI GCNGC 1 cut(s) 151
PleI GAGTC 2 cut(s) 170, 275
PpsI GAGTC 2 cut(s) 170, 275
PshAI GACNNNNGTC 1 cut(s) 177
PsiI TTATAA 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 84
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
SapI GCTCTTC 1 cut(s) 30
SaqAI TTAA 2 cut(s) 336, 377
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 1 cut(s) 123
ScaI AGTACT 1 cut(s) 90
SchI GAGTC 2 cut(s) 171, 276
SduI GDGCHC 1 cut(s) 85
SetI ASST 5 cut(s) 74, 144, 215, 311, 329
SfaNI GCATC 1 cut(s) 136
SfcI CTRYAG 1 cut(s) 76
Sse9I AATT 4 cut(s) 18, 337, 364, 378
TaiI ACGT 2 cut(s) 144, 215
TaqI TCGA 1 cut(s) 189
TasI AATT 4 cut(s) 18, 337, 364, 378
TatI WGTACW 1 cut(s) 88
Tru1I TTAA 2 cut(s) 336, 377
Tru9I TTAA 2 cut(s) 336, 377
TseI GCWGC 1 cut(s) 149
TspDTI ATGAA 4 cut(s) 17, 263, 307, 360
XagI CCTNNNNNAGG 1 cut(s) 76
XmiI GTMKAC 2 cut(s) 54, 165
ZrmI AGTACT 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.