Rh2AG280400

Alginate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000532
Physical Location & Seq
Forward (+)
33948596 .. 33974355
25760 bp
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UTR
Exon/CDS
Intron
Rh2AG280400.1

Sequence Viewer

Length: 393 bp
ATGAAGAGTACCACCCAGGGATACGATTACTCTTCTGGTGTGTGGAAATTTGAAGCATATGCGCAAGTGCCAAATGGAACATCAGGGGTCTGCATAATGCAAGTGTTTCGAGCAATTGCTCTTCATGCCTCAACTCTAATGTTGAGGGTCTACAATGGTTCACTCACCTACTACAGGGCTCCAGTACTGGTTACAAACATCTACGATAAGTGGTTCAAGATCAATGTGGTTCACAACGTTGATGCTGCGAAAGTCAGAGTCTACATTGACGGCTGTCTCAAAATCGAGGCAAATGGTCGTGGAGGAACGTCTAATGCTTTCAAATGTGGAGTGTATGCCCAGATGAATGACTCTATTTACATGGAGTCTCGCTGGAAAGACATCAAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

130

Amino Acids

14.64

Weight (kDa)

9.28

Isoelectric Point (pI)

27.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 8 - 130 9.1e-17 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 63
AccI GTMKAC 2 cut(s) 150, 261
AclI AACGTT 1 cut(s) 237
AcsI RAATTY 1 cut(s) 47
AfaI GTAC 2 cut(s) 10, 186
AfiI CCNNNNNNNGG 1 cut(s) 174
AgsI TTSAA 3 cut(s) 53, 217, 322
AjnI CCWGG 1 cut(s) 15
Alw26I GTCTC 2 cut(s) 281, 372
ApeKI GCWGC 1 cut(s) 245
ApoI RAATTY 1 cut(s) 47
AspLEI GCGC 1 cut(s) 64
AsuHPI GGTGA 1 cut(s) 157
BanII GRGCYC 1 cut(s) 181
BbvI GCAGC 1 cut(s) 232
BceAI ACGGC 1 cut(s) 286
BcgI CGANNNNNNTGC 2 cut(s) 89, 123
BciT130I CCWGG 1 cut(s) 17
BciVI GTATCC 1 cut(s) 14
BcoDI GTCTC 2 cut(s) 281, 372
BfmI CTRYAG 1 cut(s) 172
BfuI GTATCC 1 cut(s) 14
BisI GCNGC 1 cut(s) 246
BlsI GCNGC 1 cut(s) 247
BmcAI AGTACT 1 cut(s) 186
Bme1390I CCNGG 1 cut(s) 17
BmiI GGNNCC 1 cut(s) 180
BmrFI CCNGG 1 cut(s) 17
BmsI GCATC 1 cut(s) 232
BoxI GACNNNNGTC 1 cut(s) 273
BpmI CTGGAG 1 cut(s) 165
BsaJI CCNNGG 2 cut(s) 15, 16
Bsc4I CCNNNNNNNGG 1 cut(s) 174
Bse1I ACTGG 2 cut(s) 182, 192
BseBI CCWGG 1 cut(s) 17
BseDI CCNNGG 2 cut(s) 15, 16
BseLI CCNNNNNNNGG 1 cut(s) 174
BseNI ACTGG 2 cut(s) 182, 192
BseXI GCAGC 1 cut(s) 232
BslI CCNNNNNNNGG 1 cut(s) 174
BsmAI GTCTC 2 cut(s) 281, 372
Bsp1286I GDGCHC 1 cut(s) 181
Bsp143I GATC 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 180
BspQI GCTCTTC 1 cut(s) 126
BsrI ACTGG 2 cut(s) 182, 192
BssECI CCNNGG 2 cut(s) 15, 16
BssMI GATC 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 17
Bst6I CTCTTC 2 cut(s) 37, 126
BstENI CCTNNNNNAGG 1 cut(s) 172
BstHHI GCGC 1 cut(s) 64
BstKTI GATC 1 cut(s) 222
BstMAI GTCTC 2 cut(s) 281, 372
BstMBI GATC 1 cut(s) 219
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstNI CCWGG 1 cut(s) 17
BstPAI GACNNNNGTC 1 cut(s) 273
BstSCI CCNGG 1 cut(s) 15
BstSFI CTRYAG 1 cut(s) 172
BstV1I GCAGC 1 cut(s) 232
BsuI GTATCC 1 cut(s) 14
CfoI GCGC 1 cut(s) 64
Csp6I GTAC 2 cut(s) 9, 185
CviAII CATG 2 cut(s) 125, 361
CviJI RGCY 2 cut(s) 179, 273
CviKI_1 RGCY 2 cut(s) 179, 273
CviQI GTAC 2 cut(s) 9, 185
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
Eam1104I CTCTTC 2 cut(s) 37, 126
EarI CTCTTC 2 cut(s) 37, 126
Eco24I GRGCYC 1 cut(s) 181
EcoNI CCTNNNNNAGG 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 15
EcoT38I GRGCYC 1 cut(s) 181
FaeI CATG 2 cut(s) 128, 364
FaiI YATR 6 cut(s) 58, 60, 95, 126, 336, 362
FatI CATG 2 cut(s) 124, 360
FauNDI CATATG 1 cut(s) 58
FblI GTMKAC 2 cut(s) 150, 261
Fnu4HI GCNGC 1 cut(s) 246
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 1 cut(s) 246
FspI TGCGCA 1 cut(s) 63
GlaI GCGC 1 cut(s) 63
GluI GCNGC 1 cut(s) 246
GsuI CTGGAG 1 cut(s) 165
HhaI GCGC 1 cut(s) 64
Hin1II CATG 2 cut(s) 128, 364
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HinfI GANTC 3 cut(s) 258, 350, 365
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 4 cut(s) 151, 161, 232, 262
Hpy188I TCNGA 1 cut(s) 257
Hpy188III TCNNGA 1 cut(s) 217
Hpy8I GTNNAC 4 cut(s) 151, 161, 232, 262
HpyCH4IV ACGT 2 cut(s) 237, 308
HpyCH4V TGCA 2 cut(s) 93, 100
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpySE526I ACGT 2 cut(s) 237, 308
Hsp92II CATG 2 cut(s) 128, 364
HspAI GCGC 1 cut(s) 62
Kzo9I GATC 1 cut(s) 219
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 184
LpnPI CCDG 9 cut(s) 2, 21, 29, 69, 160, 173, 195, 353, 358
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 1 cut(s) 232
MaeII ACGT 2 cut(s) 237, 308
MaeIII GTNAC 1 cut(s) 190
MalI GATC 1 cut(s) 221
MboI GATC 1 cut(s) 219
MboII GAAGA 3 cut(s) 16, 24, 113
MfeI CAATTG 1 cut(s) 114
MhlI GDGCHC 1 cut(s) 181
MluCI AATT 2 cut(s) 47, 114
MlyI GAGTC 3 cut(s) 267, 344, 374
MnlI CCTC 4 cut(s) 138, 139, 280, 296
MspR9I CCNGG 1 cut(s) 17
MunI CAATTG 1 cut(s) 114
MvaI CCWGG 1 cut(s) 17
MwoI GCNNNNNNNGC 1 cut(s) 125
NdeI CATATG 1 cut(s) 58
NdeII GATC 1 cut(s) 219
NlaIII CATG 2 cut(s) 128, 364
NlaIV GGNNCC 1 cut(s) 180
NsbI TGCGCA 1 cut(s) 63
PasI CCCWGGG 1 cut(s) 16
PciSI GCTCTTC 1 cut(s) 126
PkrI GCNGC 1 cut(s) 247
PleI GAGTC 3 cut(s) 266, 344, 373
PpsI GAGTC 3 cut(s) 266, 344, 373
PshAI GACNNNNGTC 1 cut(s) 273
Psp1406I AACGTT 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 15
PspGI CCWGG 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 180
RsaI GTAC 2 cut(s) 10, 186
RsaNI GTAC 2 cut(s) 9, 185
SapI GCTCTTC 1 cut(s) 126
SatI GCNGC 1 cut(s) 246
Sau3AI GATC 1 cut(s) 219
ScaI AGTACT 1 cut(s) 186
SchI GAGTC 3 cut(s) 267, 344, 374
ScrFI CCNGG 1 cut(s) 17
SduI GDGCHC 1 cut(s) 181
SetI ASST 3 cut(s) 170, 240, 311
SfaNI GCATC 1 cut(s) 232
SfcI CTRYAG 1 cut(s) 172
Sse9I AATT 2 cut(s) 47, 114
StyD4I CCNGG 1 cut(s) 15
TaiI ACGT 2 cut(s) 240, 311
TaqI TCGA 2 cut(s) 109, 285
TasI AATT 2 cut(s) 47, 114
TatI WGTACW 1 cut(s) 184
TseI GCWGC 1 cut(s) 245
TspDTI ATGAA 3 cut(s) 17, 113, 359
XagI CCTNNNNNAGG 1 cut(s) 172
XapI RAATTY 1 cut(s) 47
XmiI GTMKAC 2 cut(s) 150, 261
ZrmI AGTACT 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.