Rh2DG306100

Alginate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000532
Physical Location & Seq
Forward (+)
37265992 .. 37266264
273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG306100.1

Sequence Viewer

Length: 273 bp
ATGCTGAGGGTCTACAATGGTTCACTAACATACTACATGGCTCCGGTGTTGGTTCCCAACATCTACGATAAGTGGTTCAAGCTCAATGTGGTTCACGATGTTGATGGTGCAAAAGTCAGAGTGTACATTGATAGATGTCTCAAAATCGAAGCAGATGGTCGTGGAGGAACCTCTCATGCTTTCAAATGTGGTGTCTACGCCCAGATGAATGACTCCAATTACATGGAGTCTCGTTGGAAACACATCAAAGTTTTAAGAAAGTGTGGACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

90

Amino Acids

10.46

Weight (kDa)

9.51

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 3 - 84 3e-09 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 12, 195
AfaI GTAC 1 cut(s) 125
AgsI TTSAA 2 cut(s) 79, 184
AluBI AGCT 1 cut(s) 82
AluI AGCT 1 cut(s) 82
Alw26I GTCTC 2 cut(s) 143, 234
ArsI GACNNNNNNTTYG 2 cut(s) 177, 209
BbvCI CCTCAGC 1 cut(s) 5
BccI CCATC 2 cut(s) 98, 149
BcoDI GTCTC 2 cut(s) 143, 234
BmiI GGNNCC 3 cut(s) 42, 54, 169
Bpu10I CCTNAGC 1 cut(s) 5
BsaWI WCCGGW 1 cut(s) 43
BsiSI CCGG 1 cut(s) 44
BsmAI GTCTC 2 cut(s) 143, 234
Bsp1407I TGTACA 1 cut(s) 123
BspLI GGNNCC 3 cut(s) 42, 54, 169
BsrGI TGTACA 1 cut(s) 123
BstAUI TGTACA 1 cut(s) 123
BstDEI CTNAG 1 cut(s) 5
BstMAI GTCTC 2 cut(s) 143, 234
BstXI CCANNNNNNTGG 1 cut(s) 223
Csp6I GTAC 1 cut(s) 124
CviAII CATG 3 cut(s) 37, 176, 223
CviJI RGCY 2 cut(s) 41, 82
CviKI_1 RGCY 2 cut(s) 41, 82
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 1 cut(s) 5
FaeI CATG 3 cut(s) 40, 179, 226
FaiI YATR 4 cut(s) 31, 38, 177, 224
FatI CATG 3 cut(s) 36, 175, 222
FblI GTMKAC 2 cut(s) 12, 195
HapII CCGG 1 cut(s) 44
Hin1II CATG 3 cut(s) 40, 179, 226
HinfI GANTC 2 cut(s) 212, 227
HpaII CCGG 1 cut(s) 44
Hpy166II GTNNAC 6 cut(s) 13, 23, 94, 124, 196, 266
Hpy188I TCNGA 1 cut(s) 119
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 6 cut(s) 13, 23, 94, 124, 196, 266
HpyCH4IV ACGT 1 cut(s) 268
HpyCH4V TGCA 1 cut(s) 110
HpyF3I CTNAG 1 cut(s) 5
HpySE526I ACGT 1 cut(s) 268
Hsp92II CATG 3 cut(s) 40, 179, 226
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 2 cut(s) 57, 215
MaeII ACGT 1 cut(s) 268
MluCI AATT 1 cut(s) 217
MlyI GAGTC 2 cut(s) 206, 236
MmeI TCCRAC 1 cut(s) 215
MnlI CCTC 2 cut(s) 158, 181
MseI TTAA 1 cut(s) 254
MspI CCGG 1 cut(s) 44
NlaIII CATG 3 cut(s) 40, 179, 226
NlaIV GGNNCC 3 cut(s) 42, 54, 169
PleI GAGTC 2 cut(s) 206, 235
PpsI GAGTC 2 cut(s) 206, 235
PspN4I GGNNCC 3 cut(s) 42, 54, 169
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SaqAI TTAA 1 cut(s) 254
SchI GAGTC 2 cut(s) 206, 236
SetI ASST 3 cut(s) 84, 173, 271
SgeI CNNG 9 cut(s) 49, 56, 91, 107, 173, 188, 214, 235, 243
Sse9I AATT 1 cut(s) 217
TaiI ACGT 1 cut(s) 271
TaqI TCGA 1 cut(s) 147
TasI AATT 1 cut(s) 217
TatI WGTACW 1 cut(s) 123
Tru1I TTAA 1 cut(s) 254
Tru9I TTAA 1 cut(s) 254
TspDTI ATGAA 1 cut(s) 221
XmiI GTMKAC 2 cut(s) 12, 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.