RLG00000018485

Alginate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000532
Physical Location & Seq
Forward (+)
30650672 .. 30651455
784 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018485

Sequence Viewer

Length: 690 bp
ATGGCATACTCCTTTCACTCCCTAGCGTTCTTCTGCTCAAGCATTCTAATATTCAAGCTTATCAGTCTTCACCAGTCAGCCTTGGCTTGGAGTTGGGGACCTGATGTTCCTCCTACACAGGGCTTTGTCTCCCTCCCTTTCAACCGATCCTACTACCATATTCAGAAACCTTACGATGTGCCTGAAGACCAGCGCTACTGCTTCATCGATGGAGTTCATAAATGTTGGGTCTACTCCACAGACAAACCTCACACTACTACCAGCCAAACCCTGCCTCGCACTGAGATTGCCATACAAGGATACAATTACTCTTCTGGGGTGTGGGAGTTCGAAGCATTTGGGTACGTCCCAAATGGGACATCAGGGGTGTGCATAATGCAAGTGTTTGGAGCAAGTGTTCCTTATGCCACAACTCTGATGCTGAGGGTCTACAATGGTTCACTAACATACTACATGGCTCCGGTGTTGGTTCCCAACATCTACGATAAGTGGTTCAAGCTCAATGTGGTTCACGATGTTGATGCTGCAAAAGTCAGAGTGTACATTGATGGATGTCTCAAAATCGAAGCAGATGGTCGTGGAGGAATCGCTCATGCTTTCAAATGTGGTGTCTATGCCCAGATGAGTGACTCCAATTACATGGAGTCTCGTTGGAAACACATCAAAGTTTTAAGAAAGTGTGGACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

230

Amino Acids

26.04

Weight (kDa)

8.59

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 50 - 223 4.4e-25 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 231, 429
AclWI GGATC 1 cut(s) 141
AcuI CTGAAG 1 cut(s) 204
AfaI GTAC 2 cut(s) 344, 542
AfeI AGCGCT 1 cut(s) 194
AfiI CCNNNNNNNGG 2 cut(s) 87, 119
AgsI TTSAA 4 cut(s) 55, 142, 496, 601
AloI GAACNNNNNNTCC 4 cut(s) 90, 122, 381, 413
AluBI AGCT 2 cut(s) 58, 499
AluI AGCT 2 cut(s) 58, 499
Alw26I GTCTC 3 cut(s) 133, 560, 651
AlwI GGATC 1 cut(s) 141
Aor51HI AGCGCT 1 cut(s) 194
ApeKI GCWGC 1 cut(s) 524
ArsI GACNNNNNNTTYG 2 cut(s) 594, 626
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 62
AsuII TTCGAA 1 cut(s) 330
AvaII GGWCC 1 cut(s) 98
BbsI GAAGAC 2 cut(s) 59, 192
BbvCI CCTCAGC 1 cut(s) 422
BbvI GCAGC 1 cut(s) 511
BccI CCATC 3 cut(s) 203, 542, 566
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciVI GTATCC 1 cut(s) 293
BcoDI GTCTC 3 cut(s) 133, 560, 651
BfaI CTAG 1 cut(s) 23
BfoI RGCGCY 1 cut(s) 196
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 1 cut(s) 525
BlsI GCNGC 1 cut(s) 526
Bme18I GGWCC 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 3 cut(s) 99, 459, 471
BmsI GCATC 2 cut(s) 408, 511
BpiI GAAGAC 2 cut(s) 59, 192
Bpu10I CCTNAGC 1 cut(s) 422
Bpu14I TTCGAA 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 22
Bsa29I ATCGAT 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 81
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 119
Bse1I ACTGG 1 cut(s) 73
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 81
BseGI GGATG 1 cut(s) 557
BseLI CCNNNNNNNGG 2 cut(s) 87, 119
BseMII CTCAG 2 cut(s) 273, 413
BseNI ACTGG 1 cut(s) 73
BseXI GCAGC 1 cut(s) 511
BshVI ATCGAT 1 cut(s) 207
BsiSI CCGG 1 cut(s) 461
BslFI GGGAC 3 cut(s) 111, 332, 370
BslI CCNNNNNNNGG 2 cut(s) 87, 119
BsmAI GTCTC 3 cut(s) 133, 560, 651
BsmFI GGGAC 3 cut(s) 111, 332, 370
BsmI GAATGC 1 cut(s) 42
Bsp119I TTCGAA 1 cut(s) 330
Bsp1407I TGTACA 1 cut(s) 540
Bsp143I GATC 1 cut(s) 146
BspCNI CTCAG 2 cut(s) 274, 414
BspDI ATCGAT 1 cut(s) 207
BspLI GGNNCC 3 cut(s) 99, 459, 471
BspPI GGATC 1 cut(s) 141
BspT104I TTCGAA 1 cut(s) 330
BsrGI TGTACA 1 cut(s) 540
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 81
Bst6I CTCTTC 1 cut(s) 316
BstAUI TGTACA 1 cut(s) 540
BstBI TTCGAA 1 cut(s) 330
BstDEI CTNAG 2 cut(s) 282, 422
BstF5I GGATG 1 cut(s) 557
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 1 cut(s) 149
BstMAI GTCTC 3 cut(s) 133, 560, 651
BstMBI GATC 1 cut(s) 146
BstV1I GCAGC 1 cut(s) 511
BstV2I GAAGAC 2 cut(s) 59, 192
BstXI CCANNNNNNTGG 1 cut(s) 640
Bsu15I ATCGAT 1 cut(s) 207
BsuI GTATCC 1 cut(s) 293
BsuTUI ATCGAT 1 cut(s) 207
BtsCI GGATG 1 cut(s) 557
BtsIMutI CAGTG 1 cut(s) 279
CfoI GCGC 1 cut(s) 195
Cfr13I GGNCC 1 cut(s) 98
ClaI ATCGAT 1 cut(s) 207
Csp6I GTAC 2 cut(s) 343, 541
CviAII CATG 3 cut(s) 454, 593, 640
CviJI RGCY 7 cut(s) 58, 80, 86, 123, 264, 458, 499
CviKI_1 RGCY 7 cut(s) 58, 80, 86, 123, 264, 458, 499
CviQI GTAC 2 cut(s) 343, 541
DdeI CTNAG 2 cut(s) 282, 422
DpnI GATC 1 cut(s) 148
DpnII GATC 1 cut(s) 146
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
Eco130I CCWWGG 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 98
Eco47III AGCGCT 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 204
EcoO109I RGGNCCY 1 cut(s) 98
EcoT14I CCWWGG 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 3 cut(s) 457, 596, 643
FalI AAGNNNNNCTT 2 cut(s) 385, 417
FaqI GGGAC 3 cut(s) 111, 332, 370
FatI CATG 3 cut(s) 453, 592, 639
FblI GTMKAC 2 cut(s) 231, 429
Fnu4HI GCNGC 1 cut(s) 525
FokI GGATG 1 cut(s) 564
Fsp4HI GCNGC 1 cut(s) 525
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 194
GluI GCNGC 1 cut(s) 525
HaeII RGCGCY 1 cut(s) 196
HapII CCGG 1 cut(s) 461
HhaI GCGC 1 cut(s) 195
Hin1II CATG 3 cut(s) 457, 596, 643
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 3 cut(s) 585, 629, 644
HpaII CCGG 1 cut(s) 461
HphI GGTGA 1 cut(s) 62
Hpy166II GTNNAC 6 cut(s) 232, 430, 440, 511, 541, 683
Hpy188I TCNGA 3 cut(s) 165, 417, 536
Hpy188III TCNNGA 1 cut(s) 512
Hpy8I GTNNAC 6 cut(s) 232, 430, 440, 511, 541, 683
HpyCH4IV ACGT 2 cut(s) 345, 685
HpyCH4V TGCA 3 cut(s) 372, 379, 527
HpyF3I CTNAG 2 cut(s) 282, 422
HpySE526I ACGT 2 cut(s) 345, 685
Hsp92II CATG 3 cut(s) 457, 596, 643
HspAI GCGC 1 cut(s) 193
Kzo9I GATC 1 cut(s) 146
LmnI GCTCC 2 cut(s) 389, 463
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 2 cut(s) 408, 511
MaeI CTAG 1 cut(s) 23
MaeII ACGT 2 cut(s) 345, 685
MaeIII GTNAC 1 cut(s) 626
MalI GATC 1 cut(s) 148
MboI GATC 1 cut(s) 146
MboII GAAGA 4 cut(s) 22, 59, 197, 303
MluCI AATT 2 cut(s) 304, 634
MlyI GAGTC 2 cut(s) 623, 653
MmeI TCCRAC 1 cut(s) 632
MnlI CCTC 6 cut(s) 120, 143, 258, 285, 417, 575
MseI TTAA 1 cut(s) 671
MspI CCGG 1 cut(s) 461
Mva1269I GAATGC 1 cut(s) 42
NdeII GATC 1 cut(s) 146
NlaIII CATG 3 cut(s) 457, 596, 643
NlaIV GGNNCC 3 cut(s) 99, 459, 471
NmuCI GTSAC 1 cut(s) 626
NspV TTCGAA 1 cut(s) 330
PctI GAATGC 1 cut(s) 42
PfeI GAWTC 1 cut(s) 585
PkrI GCNGC 1 cut(s) 526
PleI GAGTC 2 cut(s) 623, 652
PpsI GAGTC 2 cut(s) 623, 652
PpuMI RGGWCCY 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 98
PspN4I GGNNCC 3 cut(s) 99, 459, 471
PspPI GGNCC 1 cut(s) 98
PspPPI RGGWCCY 1 cut(s) 98
RsaI GTAC 2 cut(s) 344, 542
RsaNI GTAC 2 cut(s) 343, 541
SaqAI TTAA 1 cut(s) 671
SatI GCNGC 1 cut(s) 525
Sau3AI GATC 1 cut(s) 146
Sau96I GGNCC 1 cut(s) 98
SchI GAGTC 2 cut(s) 623, 653
SetI ASST 7 cut(s) 60, 103, 172, 250, 348, 501, 688
SfaNI GCATC 2 cut(s) 408, 511
SfuI TTCGAA 1 cut(s) 330
SinI GGWCC 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 2 cut(s) 304, 634
SspI AATATT 1 cut(s) 51
SspMI CTAG 1 cut(s) 23
StyI CCWWGG 1 cut(s) 81
TaiI ACGT 2 cut(s) 348, 688
TaqI TCGA 3 cut(s) 207, 330, 564
TasI AATT 2 cut(s) 304, 634
TatI WGTACW 1 cut(s) 540
TfiI GAWTC 1 cut(s) 585
Tru1I TTAA 1 cut(s) 671
Tru9I TTAA 1 cut(s) 671
TscAI CASTG 1 cut(s) 286
TseFI GTSAC 1 cut(s) 626
TseI GCWGC 1 cut(s) 524
Tsp45I GTSAC 1 cut(s) 626
TspDTI ATGAA 2 cut(s) 193, 206
TspRI CASTG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 98
XmiI GTMKAC 2 cut(s) 231, 429
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.