FvH4_2g13510

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000532
Physical Location & Seq
Forward (+)
11787523 .. 11788558
1036 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13510.t1

Sequence Viewer

Length: 663 bp
ATGAGAGGTTCTTACTGCTATCTGCTTCTCCTTTTTCTTTTACCCAGCTCACTGAAATATTTCAGTCAGTTATGTGGTGCTAATCCTGATCCTACCAATGGCTTCATCTCCATCCCATTATCAGAGTACAACTTCGAATTGCAGAAACCCTACGACATACCACTTGAGCAACGGTACAGTTATGTCGATGGTGTAAGGCATTTGTGGGTCTATGCTGATGACAAGCCACATGACCCGAATAGTCTTACTCAGCCACGCACTGAAGTTCGCATTCGGGGACTCGACTATTCATCTGGAGTTTGGCAATTTGAAGGGTATGGCTTTGTGCCAAATGGAACCTCTGGTGCAACCGTAGCACAGATCCATGGAGCAGCTCACGGCGCAACCACTATCATACTAAGAATCTACAATGGCGATATGAGGTATTACAGTGGAGATCTGGTGGCTACTGATCTTTATGATAGATGGTTCAGACTTAACATTGTCCATGACGTCGATGGAGGGACTGTGACTGTTTATATTGATGGACTCCAGAAGTTTCAAGTGAAGGATAAAGGGCCGGGCGACTTGTACTTCAAATGTGGAGTTTATGCTGCTCCCGCTAATATAAGCTACTACATGGAATCACGTTGGAAAGGCATCAAAATATATAAAAAGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

221

Amino Acids

25.02

Weight (kDa)

6.94

Isoelectric Point (pI)

25.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 44 - 217 1.6e-23 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 495
AciI CCGC 1 cut(s) 600
AclWI GGATC 2 cut(s) 83, 355
AcuI CTGAAG 1 cut(s) 282
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 3 cut(s) 128, 176, 572
AfiI CCNNNNNNNGG 1 cut(s) 98
AgsI TTSAA 3 cut(s) 311, 542, 577
AluBI AGCT 3 cut(s) 48, 374, 612
AluI AGCT 3 cut(s) 48, 374, 612
AlwI GGATC 2 cut(s) 83, 355
AoxI GGCC 1 cut(s) 557
ApeKI GCWGC 2 cut(s) 371, 593
Asp700I GAANNNNTTC 1 cut(s) 59
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 1 cut(s) 557
AsuC2I CCSGG 1 cut(s) 561
AsuII TTCGAA 1 cut(s) 135
BbvI GCAGC 2 cut(s) 383, 580
BccI CCATC 5 cut(s) 119, 182, 459, 491, 518
BceAI ACGGC 1 cut(s) 394
BcnI CCSGG 1 cut(s) 561
BglII AGATCT 1 cut(s) 436
BisI GCNGC 2 cut(s) 372, 594
BlsI GCNGC 2 cut(s) 373, 595
Bme1390I CCNGG 1 cut(s) 561
BmgT120I GGNCC 1 cut(s) 557
BmiI GGNNCC 1 cut(s) 337
BmrFI CCNGG 1 cut(s) 561
BmsI GCATC 1 cut(s) 648
BpmI CTGGAG 2 cut(s) 315, 515
Bpu14I TTCGAA 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 1 cut(s) 561
BsaHI GRCGYC 1 cut(s) 492
BsaJI CCNNGG 1 cut(s) 364
BsaXI ACNNNNNCTCC 4 cut(s) 426, 456, 492, 522
Bsc4I CCNNNNNNNGG 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 364
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 98
BseMII CTCAG 1 cut(s) 263
BseXI GCAGC 2 cut(s) 383, 580
BseYI CCCAGC 1 cut(s) 44
BshFI GGCC 1 cut(s) 559
BsiSI CCGG 1 cut(s) 560
BslFI GGGAC 2 cut(s) 291, 517
BslI CCNNNNNNNGG 1 cut(s) 98
BsmFI GGGAC 2 cut(s) 291, 517
BsmI GAATGC 1 cut(s) 270
BsnI GGCC 1 cut(s) 559
Bsp119I TTCGAA 1 cut(s) 135
Bsp143I GATC 4 cut(s) 88, 360, 436, 451
Bsp19I CCATGG 1 cut(s) 364
BspACI CCGC 1 cut(s) 600
BspANI GGCC 1 cut(s) 559
BspCNI CTCAG 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 337
BspPI GGATC 2 cut(s) 83, 355
BspT104I TTCGAA 1 cut(s) 135
BssECI CCNNGG 1 cut(s) 364
BssMI GATC 4 cut(s) 88, 360, 436, 451
BssNI GRCGYC 1 cut(s) 492
BssT1I CCWWGG 1 cut(s) 364
Bst4CI ACNGT 6 cut(s) 174, 179, 352, 431, 508, 514
BstACI GRCGYC 1 cut(s) 492
BstBI TTCGAA 1 cut(s) 135
BstDEI CTNAG 2 cut(s) 249, 398
BstDSI CCRYGG 1 cut(s) 364
BstF5I GGATG 1 cut(s) 111
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 4 cut(s) 91, 363, 439, 454
BstMBI GATC 4 cut(s) 88, 360, 436, 451
BstMWI GCNNNNNNNGC 3 cut(s) 353, 380, 599
BstSCI CCNGG 1 cut(s) 559
BstV1I GCAGC 2 cut(s) 383, 580
BstX2I RGATCY 2 cut(s) 360, 436
BstYI RGATCY 2 cut(s) 360, 436
BsuRI GGCC 1 cut(s) 559
BtgI CCRYGG 1 cut(s) 364
BtsCI GGATG 1 cut(s) 111
BtsIMutI CAGTG 3 cut(s) 50, 258, 436
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 557
Csp6I GTAC 3 cut(s) 127, 175, 571
CviAII CATG 4 cut(s) 230, 365, 488, 619
CviJI RGCY 9 cut(s) 48, 102, 226, 253, 321, 374, 446, 559, 612
CviKI_1 RGCY 9 cut(s) 48, 102, 226, 253, 321, 374, 446, 559, 612
CviQI GTAC 3 cut(s) 127, 175, 571
DdeI CTNAG 2 cut(s) 249, 398
DpnI GATC 4 cut(s) 90, 362, 438, 453
DpnII GATC 4 cut(s) 88, 360, 436, 451
Eco130I CCWWGG 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 282
EcoT14I CCWWGG 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 364
FaeI CATG 4 cut(s) 233, 368, 491, 622
FaqI GGGAC 2 cut(s) 291, 517
FatI CATG 4 cut(s) 229, 364, 487, 618
FauI CCCGC 1 cut(s) 607
Fnu4HI GCNGC 2 cut(s) 372, 594
FokI GGATG 1 cut(s) 98
Fsp4HI GCNGC 2 cut(s) 372, 594
GlaI GCGC 1 cut(s) 382
GluI GCNGC 2 cut(s) 372, 594
GsaI CCCAGC 1 cut(s) 48
GsuI CTGGAG 2 cut(s) 315, 515
HaeIII GGCC 1 cut(s) 559
HapII CCGG 1 cut(s) 560
HhaI GCGC 1 cut(s) 383
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 4 cut(s) 233, 368, 491, 622
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HinfI GANTC 4 cut(s) 279, 402, 528, 623
HpaII CCGG 1 cut(s) 560
Hpy188I TCNGA 2 cut(s) 124, 473
Hpy188III TCNNGA 3 cut(s) 86, 294, 532
Hpy99I CGWCG 1 cut(s) 497
HpyAV CCTTC 2 cut(s) 305, 541
HpyCH4III ACNGT 6 cut(s) 174, 179, 352, 431, 508, 514
HpyCH4IV ACGT 2 cut(s) 492, 628
HpyCH4V TGCA 2 cut(s) 142, 347
HpyF10VI GCNNNNNNNGC 3 cut(s) 353, 380, 599
HpyF3I CTNAG 2 cut(s) 249, 398
HpySE526I ACGT 2 cut(s) 492, 628
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 4 cut(s) 233, 368, 491, 622
HspAI GCGC 1 cut(s) 381
Kzo9I GATC 4 cut(s) 88, 360, 436, 451
LmnI GCTCC 2 cut(s) 368, 601
LpnPI CCDG 7 cut(s) 58, 99, 279, 327, 425, 545, 573
Lsp1109I GCAGC 2 cut(s) 383, 580
LweI GCATC 1 cut(s) 648
MaeII ACGT 2 cut(s) 492, 628
MaeIII GTNAC 1 cut(s) 508
MalI GATC 4 cut(s) 90, 362, 438, 453
MboI GATC 4 cut(s) 88, 360, 436, 451
MflI RGATCY 2 cut(s) 360, 436
MluCI AATT 2 cut(s) 137, 305
MlyI GAGTC 2 cut(s) 273, 522
MmeI TCCRAC 1 cut(s) 611
MnlI CCTC 3 cut(s) 349, 414, 494
MroXI GAANNNNTTC 1 cut(s) 59
MseI TTAA 2 cut(s) 477, 661
MspI CCGG 1 cut(s) 560
MspR9I CCNGG 1 cut(s) 561
Mva1269I GAATGC 1 cut(s) 270
MwoI GCNNNNNNNGC 3 cut(s) 353, 380, 599
NciI CCSGG 1 cut(s) 561
NcoI CCATGG 1 cut(s) 364
NdeII GATC 4 cut(s) 88, 360, 436, 451
NlaIII CATG 4 cut(s) 233, 368, 491, 622
NlaIV GGNNCC 1 cut(s) 337
NmuCI GTSAC 1 cut(s) 508
NspV TTCGAA 1 cut(s) 135
PctI GAATGC 1 cut(s) 270
PdmI GAANNNNTTC 1 cut(s) 59
PfeI GAWTC 2 cut(s) 402, 623
PkrI GCNGC 2 cut(s) 373, 595
PleI GAGTC 2 cut(s) 273, 522
PpsI GAGTC 2 cut(s) 273, 522
PspFI CCCAGC 1 cut(s) 44
PspN4I GGNNCC 1 cut(s) 337
PspPI GGNCC 1 cut(s) 557
PsuI RGATCY 2 cut(s) 360, 436
RsaI GTAC 3 cut(s) 128, 176, 572
RsaNI GTAC 3 cut(s) 127, 175, 571
SaqAI TTAA 2 cut(s) 477, 661
SatI GCNGC 2 cut(s) 372, 594
Sau3AI GATC 4 cut(s) 88, 360, 436, 451
Sau96I GGNCC 1 cut(s) 557
SchI GAGTC 2 cut(s) 273, 522
ScrFI CCNGG 1 cut(s) 561
SetI ASST 8 cut(s) 10, 50, 341, 376, 425, 495, 614, 631
SfaNI GCATC 1 cut(s) 648
SfuI TTCGAA 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 2 cut(s) 137, 305
SsiI CCGC 1 cut(s) 600
SspI AATATT 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 559
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 6 cut(s) 174, 179, 352, 431, 508, 514
TaiI ACGT 2 cut(s) 495, 631
TaqI TCGA 4 cut(s) 135, 186, 282, 495
TasI AATT 2 cut(s) 137, 305
TatI WGTACW 2 cut(s) 126, 570
TfiI GAWTC 2 cut(s) 402, 623
Tru1I TTAA 2 cut(s) 477, 661
Tru9I TTAA 2 cut(s) 477, 661
TscAI CASTG 3 cut(s) 57, 265, 436
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 2 cut(s) 371, 593
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 2 cut(s) 94, 279
TspRI CASTG 3 cut(s) 57, 265, 436
XcmI CCANNNNNNNNNTGG 1 cut(s) 494
XmnI GAANNNNTTC 1 cut(s) 59
ZraI GACGTC 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.