MD10G1027700.v1.1

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000532
Physical Location & Seq
Reverse (-)
3584562 .. 3586318
1757 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1027700.v1.1.491

Sequence Viewer

Length: 399 bp
ATGTGGGTCTATGCTAGGGATGGGCAATGGTTATGCGGGCGGCTAGAGCTAAGTTTTATGGAGGGACTTGACTACTCATCGGGAATATGGCAATTTGAAGGGTATGGATTTGTGCCAAATGCAACCTCTGGTGCTACAGTTGCACAGCTCCATGGAGCAGCTAAGGGTGCTACGGCTACAATCCTAAGAATATATAACGGCGGCATGAGGTATTATAGTCGAGATTTGGTGGCTACAAATCTTTACGACACGAGATGCAAACTTAACATAATCCATGACGTTGACGGAGGGAGCGTGATTGTTTTCATTGACGGAGTCCAGAAATTTTGGGTGAAGGATCAAGGGCCAGGAGATTTGTTTACTTCAAATGTGGAGTTCATGCTGCACCGGCTAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

133

Amino Acids

14.75

Weight (kDa)

5.83

Isoelectric Point (pI)

13.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 36, 40, 201
AclWI GGATC 1 cut(s) 345
AcsI RAATTY 1 cut(s) 323
AgsI TTSAA 2 cut(s) 98, 366
AjnI CCWGG 1 cut(s) 346
AluBI AGCT 3 cut(s) 49, 148, 161
AluI AGCT 3 cut(s) 49, 148, 161
AlwI GGATC 1 cut(s) 345
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 2 cut(s) 158, 382
ApoI RAATTY 1 cut(s) 323
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 1 cut(s) 343
BauI CACGAG 1 cut(s) 250
BbvI GCAGC 2 cut(s) 170, 369
BccI CCATC 1 cut(s) 14
BceAI ACGGC 2 cut(s) 189, 214
BciT130I CCWGG 1 cut(s) 348
BfaI CTAG 2 cut(s) 15, 44
BfmI CTRYAG 1 cut(s) 135
BisI GCNGC 4 cut(s) 41, 159, 202, 383
BlsI GCNGC 4 cut(s) 42, 160, 203, 384
Bme1390I CCNGG 1 cut(s) 348
BmgT120I GGNCC 1 cut(s) 344
BmrFI CCNGG 1 cut(s) 348
BmsI GCATC 1 cut(s) 245
Bpu10I CCTNAGC 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 151
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bse118I RCCGGY 1 cut(s) 387
Bse3DI GCAATG 1 cut(s) 32
BseBI CCWGG 1 cut(s) 348
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 1 cut(s) 25
BseMI GCAATG 1 cut(s) 32
BseXI GCAGC 2 cut(s) 170, 369
BsgI GTGCAG 1 cut(s) 368
BshFI GGCC 1 cut(s) 346
BsiSI CCGG 1 cut(s) 388
BslFI GGGAC 1 cut(s) 78
BsmFI GGGAC 1 cut(s) 78
BsnI GGCC 1 cut(s) 346
Bsp143I GATC 1 cut(s) 337
Bsp19I CCATGG 1 cut(s) 151
BspACI CCGC 3 cut(s) 36, 40, 201
BspANI GGCC 1 cut(s) 346
BspPI GGATC 1 cut(s) 345
BsrDI GCAATG 1 cut(s) 32
BsrFI RCCGGY 1 cut(s) 387
BssAI RCCGGY 1 cut(s) 387
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 1 cut(s) 337
BssSI CACGAG 1 cut(s) 250
BssT1I CCWWGG 1 cut(s) 151
Bst2BI CACGAG 1 cut(s) 250
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 1 cut(s) 139
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 3 cut(s) 50, 162, 185
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 1 cut(s) 25
BstKTI GATC 1 cut(s) 340
BstMBI GATC 1 cut(s) 337
BstMWI GCNNNNNNNGC 4 cut(s) 46, 140, 167, 388
BstNI CCWGG 1 cut(s) 348
BstSCI CCNGG 1 cut(s) 346
BstSFI CTRYAG 1 cut(s) 135
BstV1I GCAGC 2 cut(s) 170, 369
BsuRI GGCC 1 cut(s) 346
BtgI CCRYGG 1 cut(s) 151
BtsCI GGATG 1 cut(s) 25
Cac8I GCNNGC 1 cut(s) 38
Cfr10I RCCGGY 1 cut(s) 387
Cfr13I GGNCC 1 cut(s) 344
CviAII CATG 4 cut(s) 152, 205, 275, 379
CviJI RGCY 8 cut(s) 43, 49, 148, 161, 176, 233, 346, 391
CviKI_1 RGCY 8 cut(s) 43, 49, 148, 161, 176, 233, 346, 391
DdeI CTNAG 3 cut(s) 50, 162, 185
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 151
EcoRII CCWGG 1 cut(s) 346
EcoT14I CCWWGG 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 151
FaeI CATG 4 cut(s) 155, 208, 278, 382
FaqI GGGAC 1 cut(s) 78
FatI CATG 4 cut(s) 151, 204, 274, 378
FauI CCCGC 1 cut(s) 29
Fnu4HI GCNGC 4 cut(s) 41, 159, 202, 383
FokI GGATG 1 cut(s) 32
Fsp4HI GCNGC 4 cut(s) 41, 159, 202, 383
FspBI CTAG 2 cut(s) 15, 44
GluI GCNGC 4 cut(s) 41, 159, 202, 383
HaeIII GGCC 1 cut(s) 346
HapII CCGG 1 cut(s) 388
Hin1II CATG 4 cut(s) 155, 208, 278, 382
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 1 cut(s) 315
HpaII CCGG 1 cut(s) 388
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 2 cut(s) 283, 360
Hpy188III TCNNGA 3 cut(s) 81, 221, 319
Hpy8I GTNNAC 2 cut(s) 283, 360
HpyAV CCTTC 2 cut(s) 92, 328
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 4 cut(s) 122, 143, 258, 385
HpyF10VI GCNNNNNNNGC 4 cut(s) 46, 140, 167, 388
HpyF3I CTNAG 3 cut(s) 50, 162, 185
HpySE526I ACGT 1 cut(s) 279
Hsp92II CATG 4 cut(s) 155, 208, 278, 382
Kzo9I GATC 1 cut(s) 337
LmnI GCTCC 3 cut(s) 153, 155, 291
LpnPI CCDG 4 cut(s) 114, 332, 333, 360
Lsp1109I GCAGC 2 cut(s) 170, 369
LweI GCATC 1 cut(s) 245
MaeI CTAG 2 cut(s) 15, 44
MaeII ACGT 1 cut(s) 279
MalI GATC 1 cut(s) 339
MboI GATC 1 cut(s) 337
MluCI AATT 2 cut(s) 92, 323
MlyI GAGTC 1 cut(s) 324
MnlI CCTC 4 cut(s) 55, 136, 201, 281
MseI TTAA 1 cut(s) 264
MspI CCGG 1 cut(s) 388
MspR9I CCNGG 1 cut(s) 348
MvaI CCWGG 1 cut(s) 348
MwoI GCNNNNNNNGC 4 cut(s) 46, 140, 167, 388
NcoI CCATGG 1 cut(s) 151
NdeII GATC 1 cut(s) 337
NlaIII CATG 4 cut(s) 155, 208, 278, 382
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PflFI GACNNNGTC 1 cut(s) 314
PkrI GCNGC 4 cut(s) 42, 160, 203, 384
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 346
PspGI CCWGG 1 cut(s) 346
PspPI GGNCC 1 cut(s) 344
PsyI GACNNNGTC 1 cut(s) 314
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 4 cut(s) 41, 159, 202, 383
Sau3AI GATC 1 cut(s) 337
Sau96I GGNCC 1 cut(s) 344
SchI GAGTC 1 cut(s) 324
ScrFI CCNGG 1 cut(s) 348
SetI ASST 6 cut(s) 51, 128, 150, 163, 212, 282
SfaNI GCATC 1 cut(s) 245
SfcI CTRYAG 1 cut(s) 135
Sse9I AATT 2 cut(s) 92, 323
SsiI CCGC 3 cut(s) 36, 40, 201
SspMI CTAG 2 cut(s) 15, 44
StyD4I CCNGG 1 cut(s) 346
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 139
TaiI ACGT 1 cut(s) 282
TaqI TCGA 1 cut(s) 220
TasI AATT 2 cut(s) 92, 323
TauI GCSGC 2 cut(s) 43, 204
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TseI GCWGC 2 cut(s) 158, 382
TspDTI ATGAA 2 cut(s) 295, 367
TspGWI ACGGA 2 cut(s) 300, 327
Tth111I GACNNNGTC 1 cut(s) 314
XapI RAATTY 1 cut(s) 323
XspI CTAG 2 cut(s) 15, 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.