MD05G1029900.v1.1

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000532
Physical Location & Seq
Reverse (-)
4860639 .. 4861638
1000 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1029900.v1.1.491

Sequence Viewer

Length: 477 bp
ATGTTTATTAAGAAACCATATGACAAGGCTCCTCATGAGCGTTACAGTGATATAGATGGAGTTGAAACGTTTTGGATCTACAGCCACGACAAGCCCTTTCAAAAGCACAATCCAACTAGGCCGCGCTCCGAAATCAGAGTTTCTGGGTATGACTACACTTCCGGGGTTTGGCAATTTGAGGGCAACTTTCTTGTGCCACAAGGCACGTCGGGGGCTACAATAATGCAAATCTTTGGTTCACCCAAACAAGCTACAACTCTGCAACTAGCTGTCTACAATGGGGATTTGAAGTACTATCGTGACAATTTGCTAGCTGTCAATATCTACAACAAGTGGGTAAGGCTGAACGTGATTCACAATGTTTCTGCAAGGACAGTGACCATTTTTATCAACGGCGAACGGAAGTTGGTCAGCAAAGATCACGGCCGAGCAACCTTTTACTTTAAGTACGGAGTTTATGGAGTTGTTGACCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

159

Amino Acids

18.28

Weight (kDa)

9.44

Isoelectric Point (pI)

24.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 4 - 153 5.4e-21 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 273
AccII CGCG 1 cut(s) 124
AciI CCGC 1 cut(s) 122
AclI AACGTT 1 cut(s) 68
AclWI GGATC 1 cut(s) 83
AcoI YGGCCR 1 cut(s) 424
AfaI GTAC 2 cut(s) 293, 449
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 3 cut(s) 65, 101, 289
AjiI CACGTC 1 cut(s) 207
AluBI AGCT 3 cut(s) 251, 269, 314
AluI AGCT 3 cut(s) 251, 269, 314
AlwI GGATC 1 cut(s) 83
AoxI GGCC 2 cut(s) 119, 424
AspLEI GCGC 1 cut(s) 126
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 1 cut(s) 231
AsuNHI GCTAGC 1 cut(s) 310
BccI CCATC 1 cut(s) 50
BceAI ACGGC 2 cut(s) 409, 439
BcnI CCSGG 1 cut(s) 163
BfaI CTAG 3 cut(s) 117, 266, 311
BfmI CTRYAG 1 cut(s) 79
BisI GCNGC 1 cut(s) 122
BlsI GCNGC 1 cut(s) 123
BmcAI AGTACT 1 cut(s) 293
Bme1390I CCNGG 1 cut(s) 163
BmgBI CACGTC 1 cut(s) 207
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 1 cut(s) 163
BmtI GCTAGC 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 163
BsaJI CCNNGG 1 cut(s) 162
Bsc4I CCNNNNNNNGG 1 cut(s) 168
BseDI CCNNGG 1 cut(s) 162
BseLI CCNNNNNNNGG 1 cut(s) 168
BseRI GAGGAG 1 cut(s) 21
BseX3I CGGCCG 1 cut(s) 424
Bsh1236I CGCG 1 cut(s) 124
Bsh1285I CGRYCG 1 cut(s) 427
BshFI GGCC 2 cut(s) 121, 426
BsiEI CGRYCG 1 cut(s) 427
BsiSI CCGG 1 cut(s) 162
BslI CCNNNNNNNGG 1 cut(s) 168
BsnI GGCC 2 cut(s) 121, 426
Bsp143I GATC 2 cut(s) 75, 418
BspACI CCGC 1 cut(s) 122
BspANI GGCC 2 cut(s) 121, 426
BspFNI CGCG 1 cut(s) 124
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 1 cut(s) 30
BspOI GCTAGC 1 cut(s) 314
BspPI GGATC 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 2 cut(s) 75, 418
Bst4CI ACNGT 2 cut(s) 47, 376
BstC8I GCNNGC 1 cut(s) 312
BstFNI CGCG 1 cut(s) 124
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 2 cut(s) 78, 421
BstMBI GATC 2 cut(s) 75, 418
BstMCI CGRYCG 1 cut(s) 427
BstSCI CCNGG 1 cut(s) 161
BstSFI CTRYAG 1 cut(s) 79
BstUI CGCG 1 cut(s) 124
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BstZI CGGCCG 1 cut(s) 424
BsuRI GGCC 2 cut(s) 121, 426
BtrI CACGTC 1 cut(s) 207
BtsIMutI CAGTG 2 cut(s) 52, 381
Cac8I GCNNGC 1 cut(s) 312
CciI TCATGA 1 cut(s) 34
CfoI GCGC 1 cut(s) 126
Csp6I GTAC 2 cut(s) 292, 448
CviAII CATG 1 cut(s) 35
CviQI GTAC 2 cut(s) 292, 448
DpnI GATC 2 cut(s) 77, 420
DpnII GATC 2 cut(s) 75, 418
EaeI YGGCCR 1 cut(s) 424
EagI CGGCCG 1 cut(s) 424
EclXI CGGCCG 1 cut(s) 424
Eco52I CGGCCG 1 cut(s) 424
FaeI CATG 1 cut(s) 38
FaiI YATR 6 cut(s) 19, 21, 36, 53, 150, 459
FatI CATG 1 cut(s) 34
FauNDI CATATG 1 cut(s) 19
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 122
Fsp4HI GCNGC 1 cut(s) 122
FspBI CTAG 3 cut(s) 117, 266, 311
GlaI GCGC 1 cut(s) 125
GluI GCNGC 1 cut(s) 122
HaeIII GGCC 2 cut(s) 121, 426
HapII CCGG 1 cut(s) 162
HhaI GCGC 1 cut(s) 126
Hin1II CATG 1 cut(s) 38
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HinfI GANTC 1 cut(s) 352
HpaII CCGG 1 cut(s) 162
HphI GGTGA 1 cut(s) 231
Hpy166II GTNNAC 3 cut(s) 239, 274, 469
Hpy188I TCNGA 2 cut(s) 130, 137
Hpy188III TCNNGA 2 cut(s) 35, 299
Hpy8I GTNNAC 3 cut(s) 239, 274, 469
Hpy99I CGWCG 1 cut(s) 211
HpyCH4III ACNGT 2 cut(s) 47, 376
HpyCH4IV ACGT 3 cut(s) 68, 206, 348
HpyCH4V TGCA 3 cut(s) 226, 262, 368
HpySE526I ACGT 3 cut(s) 68, 206, 348
Hsp92II CATG 1 cut(s) 38
HspAI GCGC 1 cut(s) 124
Kzo9I GATC 2 cut(s) 75, 418
LmnI GCTCC 2 cut(s) 34, 131
LpnPI CCDG 2 cut(s) 129, 175
MaeI CTAG 3 cut(s) 117, 266, 311
MaeII ACGT 3 cut(s) 68, 206, 348
MaeIII GTNAC 3 cut(s) 41, 299, 376
MalI GATC 2 cut(s) 77, 420
MboI GATC 2 cut(s) 75, 418
MflI RGATCY 1 cut(s) 75
MluCI AATT 2 cut(s) 173, 304
MmeI TCCRAC 1 cut(s) 137
MnlI CCTC 2 cut(s) 42, 172
MseI TTAA 3 cut(s) 9, 444, 475
MspI CCGG 1 cut(s) 162
MspR9I CCNGG 1 cut(s) 163
MvnI CGCG 1 cut(s) 124
NciI CCSGG 1 cut(s) 163
NdeI CATATG 1 cut(s) 19
NdeII GATC 2 cut(s) 75, 418
NheI GCTAGC 1 cut(s) 310
NlaIII CATG 1 cut(s) 38
NlaIV GGNNCC 1 cut(s) 30
NmeAIII GCCGAG 1 cut(s) 452
NmuCI GTSAC 2 cut(s) 299, 376
PagI TCATGA 1 cut(s) 34
PfeI GAWTC 1 cut(s) 352
PkrI GCNGC 1 cut(s) 123
Psp1406I AACGTT 1 cut(s) 68
PspN4I GGNNCC 1 cut(s) 30
PsuI RGATCY 1 cut(s) 75
RsaI GTAC 2 cut(s) 293, 449
RsaNI GTAC 2 cut(s) 292, 448
SaqAI TTAA 3 cut(s) 9, 444, 475
SatI GCNGC 1 cut(s) 122
Sau3AI GATC 2 cut(s) 75, 418
ScaI AGTACT 1 cut(s) 293
ScrFI CCNGG 1 cut(s) 163
SetI ASST 7 cut(s) 71, 209, 253, 271, 316, 351, 437
SfcI CTRYAG 1 cut(s) 79
Sse9I AATT 2 cut(s) 173, 304
SsiI CCGC 1 cut(s) 122
SspMI CTAG 3 cut(s) 117, 266, 311
StyD4I CCNGG 1 cut(s) 161
TaaI ACNGT 2 cut(s) 47, 376
TaiI ACGT 3 cut(s) 71, 209, 351
TasI AATT 2 cut(s) 173, 304
TatI WGTACW 1 cut(s) 291
TauI GCSGC 1 cut(s) 124
TfiI GAWTC 1 cut(s) 352
Tru1I TTAA 3 cut(s) 9, 444, 475
Tru9I TTAA 3 cut(s) 9, 444, 475
TscAI CASTG 2 cut(s) 52, 381
TseFI GTSAC 2 cut(s) 299, 376
Tsp45I GTSAC 2 cut(s) 299, 376
TspGWI ACGGA 2 cut(s) 415, 465
TspRI CASTG 2 cut(s) 52, 381
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 3 cut(s) 117, 266, 311
ZrmI AGTACT 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.