RLG00000018486

Alginate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000532
Physical Location & Seq
Forward (+)
30654197 .. 30657249
3053 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018486

Sequence Viewer

Length: 723 bp
ATGAAGAGAGTTGAGAGTTTTTTCTCTCGAAAACCTAGCGTCGCTTCCTTTGGGTGGCTTGCAGGGAGGCCGTCCCCTCTACCAGATCTCTCCAATTTTCGCTTTGAGGAACCGCGGGCCTCCTTGTGCTTGGCTGCGCTCTTGGGTTGCCACAGGTTCAGGCTGCGCCCGTGGCAGGGATGCTGCAATGATCAAGGGAGGAGATCCGGGTTTGGGCTAGAGCGGCGCGTTCCTTCTTACAGTGATTGGCGGATTTTTGATGGCGGTACTGGGCTATTTGCTGCCGTAGTGCAGAGGGGTGTGGGACTATTAGCGGCGCCGGCGATGTGTCCAGCGATGGTTTTGGTACCCGAGTCGATGGCTTGGCTGATCGGATCCTTAATCCAGGGAGGCTGCTGGCGTAAGCCTTACGATGTGCCCGGAGACCAGCGCTACTGCTTCATCGATGGAGTTCATAAATGTGAGGGATACGATTACTCTTCTGGTGTGTGGGAATTTGAAGCATATGCGTATGTGCCAAATGGAACATCAGGAGTCTGCATAATGCAAGTGTTTGGAGCAACTGCTCCTCATGCCTCAACTCTGATGTTGAGGGTCTACAATGGTTCACTCACCTACTACAGGGCTCCAGTACTGGTTATAAACATCTACGATAAATGGTTCAAGATCAATGTGGTTCACGACGTTGATGCTGCGAAAGTCAGAGTCTACATTGACTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

241

Amino Acids

26.82

Weight (kDa)

8.8

Isoelectric Point (pI)

44.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 157 - 239 3.5e-08 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 641
Acc65I GGTACC 1 cut(s) 346
AccB1I GGYRCC 2 cut(s) 316, 346
AccBSI CCGCTC 1 cut(s) 223
AccI GTMKAC 2 cut(s) 597, 708
AccII CGCG 2 cut(s) 115, 228
AciI CCGC 6 cut(s) 113, 115, 223, 250, 264, 314
AclWI GGATC 3 cut(s) 198, 369, 382
AcsI RAATTY 1 cut(s) 494
AcyI GRCGYC 1 cut(s) 317
AfaI GTAC 3 cut(s) 268, 348, 633
AfeI AGCGCT 1 cut(s) 431
AfiI CCNNNNNNNGG 5 cut(s) 54, 175, 176, 213, 621
AgsI TTSAA 2 cut(s) 500, 664
AjnI CCWGG 1 cut(s) 384
Alw26I GTCTC 1 cut(s) 417
AlwI GGATC 3 cut(s) 198, 369, 382
Ama87I CYCGRG 1 cut(s) 350
Aor51HI AGCGCT 1 cut(s) 431
AoxI GGCC 2 cut(s) 68, 117
ApeKI GCWGC 6 cut(s) 134, 163, 183, 281, 393, 692
ApoI RAATTY 1 cut(s) 494
Asp718I GGTACC 1 cut(s) 346
AspLEI GCGC 5 cut(s) 139, 168, 228, 319, 432
AspS9I GGNCC 1 cut(s) 117
AsuC2I CCSGG 2 cut(s) 208, 420
AsuHPI GGTGA 1 cut(s) 604
AvaI CYCGRG 1 cut(s) 350
BaeGI GKGCMC 1 cut(s) 420
BaeI ACNNNNGTAYC 2 cut(s) 338, 371
BamHI GGATCC 1 cut(s) 374
BanI GGYRCC 2 cut(s) 316, 346
BanII GRGCYC 1 cut(s) 628
BbvI GCAGC 6 cut(s) 121, 150, 170, 268, 380, 679
BccI CCATC 4 cut(s) 254, 331, 352, 440
BceAI ACGGC 2 cut(s) 55, 269
BcgI CGANNNNNNTGC 2 cut(s) 671, 705
BciT130I CCWGG 1 cut(s) 386
BciVI GTATCC 1 cut(s) 461
BclI TGATCA 1 cut(s) 190
BcnI CCSGG 2 cut(s) 208, 420
BcoDI GTCTC 1 cut(s) 417
BfaI CTAG 3 cut(s) 36, 218, 721
BfmI CTRYAG 1 cut(s) 619
BfoI RGCGCY 2 cut(s) 320, 433
BfuI GTATCC 1 cut(s) 461
BglII AGATCT 1 cut(s) 85
BisI GCNGC 8 cut(s) 135, 164, 184, 224, 282, 315, 394, 693
BlsI GCNGC 8 cut(s) 136, 165, 185, 225, 283, 316, 395, 694
BmcAI AGTACT 1 cut(s) 633
Bme1390I CCNGG 3 cut(s) 208, 386, 420
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 117
BmiI GGNNCC 5 cut(s) 111, 318, 348, 376, 627
BmrFI CCNGG 3 cut(s) 208, 386, 420
BmrI ACTGGG 1 cut(s) 279
BmsI GCATC 2 cut(s) 170, 679
BmuI ACTGGG 1 cut(s) 279
BpmI CTGGAG 1 cut(s) 612
BpuMI CCSGG 2 cut(s) 208, 420
Bsa29I ATCGAT 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 317
BsaI GGTCTC 1 cut(s) 417
BsaJI CCNNGG 3 cut(s) 113, 170, 385
Bsc4I CCNNNNNNNGG 5 cut(s) 54, 175, 176, 213, 621
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 3 cut(s) 274, 629, 639
Bse3DI GCAATG 1 cut(s) 193
BseBI CCWGG 1 cut(s) 386
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 3 cut(s) 113, 170, 385
BseGI GGATG 1 cut(s) 185
BseLI CCNNNNNNNGG 5 cut(s) 54, 175, 176, 213, 621
BseMI GCAATG 1 cut(s) 193
BseNI ACTGG 3 cut(s) 274, 629, 639
BseRI GAGGAG 2 cut(s) 214, 558
BseSI GKGCMC 1 cut(s) 420
BseXI GCAGC 6 cut(s) 121, 150, 170, 268, 380, 679
BsgI GTGCAG 1 cut(s) 311
Bsh1236I CGCG 2 cut(s) 115, 228
BshFI GGCC 2 cut(s) 70, 119
BshNI GGYRCC 2 cut(s) 316, 346
BshVI ATCGAT 1 cut(s) 444
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 3 cut(s) 207, 320, 420
BslFI GGGAC 2 cut(s) 58, 318
BslI CCNNNNNNNGG 5 cut(s) 54, 175, 176, 213, 621
BsmAI GTCTC 1 cut(s) 417
BsmFI GGGAC 2 cut(s) 58, 318
BsnI GGCC 2 cut(s) 70, 119
Bso31I GGTCTC 1 cut(s) 417
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 2 cut(s) 420, 628
Bsp143I GATC 6 cut(s) 85, 190, 203, 369, 374, 666
BspACI CCGC 6 cut(s) 113, 115, 223, 250, 264, 314
BspANI GGCC 2 cut(s) 70, 119
BspDI ATCGAT 1 cut(s) 444
BspFNI CGCG 2 cut(s) 115, 228
BspLI GGNNCC 5 cut(s) 111, 318, 348, 376, 627
BspPI GGATC 3 cut(s) 198, 369, 382
BspT107I GGYRCC 2 cut(s) 316, 346
BspTNI GGTCTC 1 cut(s) 417
BsrBI CCGCTC 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 193
BsrFI RCCGGY 1 cut(s) 319
BsrI ACTGG 3 cut(s) 274, 629, 639
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 3 cut(s) 113, 170, 385
BssMI GATC 6 cut(s) 85, 190, 203, 369, 374, 666
BssNI GRCGYC 1 cut(s) 317
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 1 cut(s) 484
BstACI GRCGYC 1 cut(s) 317
BstC8I GCNNGC 4 cut(s) 60, 117, 321, 398
BstDSI CCRYGG 2 cut(s) 113, 170
BstENI CCTNNNNNAGG 1 cut(s) 619
BstF5I GGATG 1 cut(s) 185
BstFNI CGCG 2 cut(s) 115, 228
BstH2I RGCGCY 2 cut(s) 320, 433
BstHHI GCGC 5 cut(s) 139, 168, 228, 319, 432
BstKTI GATC 6 cut(s) 88, 193, 206, 372, 377, 669
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 6 cut(s) 85, 190, 203, 369, 374, 666
BstMWI GCNNNNNNNGC 4 cut(s) 172, 223, 320, 572
BstNI CCWGG 1 cut(s) 386
BstSCI CCNGG 3 cut(s) 206, 384, 418
BstSFI CTRYAG 1 cut(s) 619
BstSLI GKGCMC 1 cut(s) 420
BstUI CGCG 2 cut(s) 115, 228
BstV1I GCAGC 6 cut(s) 121, 150, 170, 268, 380, 679
BstX2I RGATCY 3 cut(s) 85, 203, 374
BstYI RGATCY 3 cut(s) 85, 203, 374
Bsu15I ATCGAT 1 cut(s) 444
BsuI GTATCC 1 cut(s) 461
BsuRI GGCC 2 cut(s) 70, 119
BsuTUI ATCGAT 1 cut(s) 444
BtgI CCRYGG 2 cut(s) 113, 170
BtgZI GCGATG 2 cut(s) 338, 350
BtsCI GGATG 1 cut(s) 185
BtsIMutI CAGTG 1 cut(s) 247
Cac8I GCNNGC 4 cut(s) 60, 117, 321, 398
CfoI GCGC 5 cut(s) 139, 168, 228, 319, 432
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 117
Cfr42I CCGCGG 1 cut(s) 116
ClaI ATCGAT 1 cut(s) 444
CseI GACGC 1 cut(s) 28
Csp6I GTAC 3 cut(s) 267, 347, 632
CviAII CATG 1 cut(s) 572
CviQI GTAC 3 cut(s) 267, 347, 632
DinI GGCGCC 1 cut(s) 318
DpnI GATC 6 cut(s) 87, 192, 205, 371, 376, 668
DpnII GATC 6 cut(s) 85, 190, 203, 369, 374, 666
Eam1104I CTCTTC 1 cut(s) 484
EarI CTCTTC 1 cut(s) 484
EciI GGCGGA 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 628
Eco31I GGTCTC 1 cut(s) 417
Eco47III AGCGCT 1 cut(s) 431
Eco88I CYCGRG 1 cut(s) 350
EcoNI CCTNNNNNAGG 1 cut(s) 619
EcoRII CCWGG 1 cut(s) 384
EcoT38I GRGCYC 1 cut(s) 628
EgeI GGCGCC 1 cut(s) 318
EheI GGCGCC 1 cut(s) 318
FaeI CATG 1 cut(s) 575
FaiI YATR 7 cut(s) 456, 505, 507, 513, 542, 573, 641
FaqI GGGAC 2 cut(s) 58, 318
FatI CATG 1 cut(s) 571
FauI CCCGC 1 cut(s) 108
FauNDI CATATG 1 cut(s) 505
FbaI TGATCA 1 cut(s) 190
FblI GTMKAC 2 cut(s) 597, 708
Fnu4HI GCNGC 8 cut(s) 135, 164, 184, 224, 282, 315, 394, 693
FokI GGATG 1 cut(s) 192
FriOI GRGCYC 1 cut(s) 628
Fsp4HI GCNGC 8 cut(s) 135, 164, 184, 224, 282, 315, 394, 693
FspBI CTAG 3 cut(s) 36, 218, 721
GlaI GCGC 5 cut(s) 138, 167, 227, 318, 431
GluI GCNGC 8 cut(s) 135, 164, 184, 224, 282, 315, 394, 693
GsuI CTGGAG 1 cut(s) 612
HaeII RGCGCY 2 cut(s) 320, 433
HaeIII GGCC 2 cut(s) 70, 119
HapII CCGG 3 cut(s) 207, 320, 420
HgaI GACGC 1 cut(s) 28
HhaI GCGC 5 cut(s) 139, 168, 228, 319, 432
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 1 cut(s) 575
Hin6I GCGC 5 cut(s) 137, 166, 226, 317, 430
HinP1I GCGC 5 cut(s) 137, 166, 226, 317, 430
HinfI GANTC 3 cut(s) 353, 534, 705
HpaII CCGG 3 cut(s) 207, 320, 420
HphI GGTGA 1 cut(s) 604
Hpy166II GTNNAC 4 cut(s) 598, 608, 679, 709
Hpy188I TCNGA 3 cut(s) 374, 585, 704
Hpy188III TCNNGA 4 cut(s) 27, 531, 664, 680
Hpy8I GTNNAC 4 cut(s) 598, 608, 679, 709
Hpy99I CGWCG 2 cut(s) 44, 686
HpyAV CCTTC 1 cut(s) 243
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 684
HpyCH4V TGCA 5 cut(s) 62, 186, 292, 540, 547
HpyF10VI GCNNNNNNNGC 4 cut(s) 172, 223, 320, 572
HpySE526I ACGT 1 cut(s) 684
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 1 cut(s) 575
HspAI GCGC 5 cut(s) 137, 166, 226, 317, 430
KasI GGCGCC 1 cut(s) 316
KpnI GGTACC 1 cut(s) 350
KroI GCCGGC 1 cut(s) 319
KroNI GCCGGC 1 cut(s) 321
Ksp22I TGATCA 1 cut(s) 190
KspI CCGCGG 1 cut(s) 116
Kzo9I GATC 6 cut(s) 85, 190, 203, 369, 374, 666
LmnI GCTCC 3 cut(s) 557, 571, 631
Lsp1109I GCAGC 6 cut(s) 121, 150, 170, 268, 380, 679
LweI GCATC 2 cut(s) 170, 679
MaeI CTAG 3 cut(s) 36, 218, 721
MaeII ACGT 1 cut(s) 684
MalI GATC 6 cut(s) 87, 192, 205, 371, 376, 668
MbiI CCGCTC 1 cut(s) 223
MboI GATC 6 cut(s) 85, 190, 203, 369, 374, 666
MboII GAAGA 2 cut(s) 16, 471
MflI RGATCY 3 cut(s) 85, 203, 374
MhlI GDGCHC 2 cut(s) 420, 628
MluCI AATT 2 cut(s) 94, 494
Mly113I GGCGCC 1 cut(s) 317
MlyI GAGTC 3 cut(s) 362, 543, 714
MreI CGCCGGCG 1 cut(s) 319
MroNI GCCGGC 1 cut(s) 319
MseI TTAA 1 cut(s) 380
MslI CAYNNNNRTG 1 cut(s) 459
MspA1I CMGCKG 1 cut(s) 115
MspI CCGG 3 cut(s) 207, 320, 420
MspR9I CCNGG 3 cut(s) 208, 386, 420
MvaI CCWGG 1 cut(s) 386
MvnI CGCG 2 cut(s) 115, 228
MwoI GCNNNNNNNGC 4 cut(s) 172, 223, 320, 572
NaeI GCCGGC 1 cut(s) 321
NarI GGCGCC 1 cut(s) 317
NciI CCSGG 2 cut(s) 208, 420
NdeI CATATG 1 cut(s) 505
NdeII GATC 6 cut(s) 85, 190, 203, 369, 374, 666
NgoMIV GCCGGC 1 cut(s) 319
NlaIII CATG 1 cut(s) 575
NlaIV GGNNCC 5 cut(s) 111, 318, 348, 376, 627
PdiI GCCGGC 1 cut(s) 321
PkrI GCNGC 8 cut(s) 136, 165, 185, 225, 283, 316, 395, 694
PleI GAGTC 3 cut(s) 361, 542, 713
PluTI GGCGCC 1 cut(s) 320
PpsI GAGTC 3 cut(s) 361, 542, 713
PsiI TTATAA 1 cut(s) 641
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 5 cut(s) 111, 318, 348, 376, 627
PspPI GGNCC 1 cut(s) 117
PsuI RGATCY 3 cut(s) 85, 203, 374
RsaI GTAC 3 cut(s) 268, 348, 633
RsaNI GTAC 3 cut(s) 267, 347, 632
RseI CAYNNNNRTG 1 cut(s) 459
SacII CCGCGG 1 cut(s) 116
SaqAI TTAA 1 cut(s) 380
SatI GCNGC 8 cut(s) 135, 164, 184, 224, 282, 315, 394, 693
Sau3AI GATC 6 cut(s) 85, 190, 203, 369, 374, 666
Sau96I GGNCC 1 cut(s) 117
ScaI AGTACT 1 cut(s) 633
SchI GAGTC 3 cut(s) 362, 543, 714
ScrFI CCNGG 3 cut(s) 208, 386, 420
SduI GDGCHC 2 cut(s) 420, 628
SetI ASST 4 cut(s) 37, 158, 617, 687
SfaNI GCATC 2 cut(s) 170, 679
SfcI CTRYAG 1 cut(s) 619
SfoI GGCGCC 1 cut(s) 318
Sfr303I CCGCGG 1 cut(s) 116
SgrAI CRCCGGYG 1 cut(s) 319
SgrBI CCGCGG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 459
Sse9I AATT 2 cut(s) 94, 494
SsiI CCGC 6 cut(s) 113, 115, 223, 250, 264, 314
SspDI GGCGCC 1 cut(s) 316
SspMI CTAG 3 cut(s) 36, 218, 721
StyD4I CCNGG 3 cut(s) 206, 384, 418
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 1 cut(s) 687
TaqI TCGA 3 cut(s) 28, 356, 444
TasI AATT 2 cut(s) 94, 494
TatI WGTACW 1 cut(s) 631
TauI GCSGC 2 cut(s) 226, 317
Tru1I TTAA 1 cut(s) 380
Tru9I TTAA 1 cut(s) 380
TscAI CASTG 1 cut(s) 247
TseI GCWGC 6 cut(s) 134, 163, 183, 281, 393, 692
TspDTI ATGAA 3 cut(s) 17, 430, 443
TspRI CASTG 1 cut(s) 247
XagI CCTNNNNNAGG 1 cut(s) 619
XapI RAATTY 1 cut(s) 494
XmiI GTMKAC 2 cut(s) 597, 708
XspI CTAG 3 cut(s) 36, 218, 721
ZrmI AGTACT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.