MD07G1183700.v1.1

Alginate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000532
Physical Location & Seq
Forward (+)
26367300 .. 26369646
2347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1183700.v1.1.491

Sequence Viewer

Length: 666 bp
ATGTATAAACTTTCGCCTGCTCCTTTTGCTATTATATTTCTTGTCTCTTCCCTGCTTGGATTTTCCTTGGTTTGGGGATCTCATGTTCCAACACAAGGGTTTGTTTCCCTCCCTTTTAACCGATCATACTACCACATTCAGAAGCCTTACGATGTTCCCGAAAATCAGCGCTACAGCTTCATCAATGGAGTTCATAAATGTTGGGTCTACTCCACGGACAAACCTCACACTACTACCAGCCAAACCCTGCCTCGTACCGAGATTGCCATACAAGGATACGGTTACTCTTCTGGTGTGTGGGAATTTGAAGCATATGGGTATGTGCCTTATGGGACATCAGGTGTGTGCATAATGCAAGTTTTCGGAGCAACTGCTCCTCATGCCACAACCCTAATGCTTAGGGTTTACAATGGCTCACTCACATACTACAGGGAAACGGTTCTGGTACCCGACATCTACGATAAATGGTTCAAGCTCAATGTGATTCACGATGTCGATGCTGCGAAAGTGAAGGTCTACATCGATGGACGCCTCAAATTAGAGGCAGATGGTCGCGGAGGGACGTCTCATGCATTCAAATGTGGTGTATACGCCCAGAATAATGACTCGTGTTATATGGAGTCTCGTTGGAAACACATTAAAGTTTTAAGGAAGTGCGGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

222

Amino Acids

25.11

Weight (kDa)

8.96

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 42 - 215 3e-29 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 566
Acc65I GGTACC 1 cut(s) 445
AccB1I GGYRCC 1 cut(s) 445
AccI GTMKAC 3 cut(s) 207, 516, 588
AccII CGCG 1 cut(s) 555
AciI CCGC 2 cut(s) 555, 657
AclWI GGATC 1 cut(s) 85
AcsI RAATTY 1 cut(s) 302
AcyI GRCGYC 2 cut(s) 529, 563
AfaI GTAC 2 cut(s) 256, 447
AfeI AGCGCT 1 cut(s) 170
AfiI CCNNNNNNNGG 2 cut(s) 72, 95
AgsI TTSAA 3 cut(s) 308, 472, 577
AloI GAACNNNNNNTCC 2 cut(s) 69, 101
AluBI AGCT 2 cut(s) 177, 475
AluI AGCT 2 cut(s) 177, 475
Alw26I GTCTC 3 cut(s) 49, 570, 627
AlwI GGATC 1 cut(s) 85
Aor51HI AGCGCT 1 cut(s) 170
ApeKI GCWGC 1 cut(s) 500
ApoI RAATTY 1 cut(s) 302
Asp700I GAANNNNTTC 1 cut(s) 438
Asp718I GGTACC 1 cut(s) 445
AspLEI GCGC 1 cut(s) 171
BanI GGYRCC 1 cut(s) 445
BauI CACGAG 1 cut(s) 607
BbvI GCAGC 1 cut(s) 487
BccI CCATC 2 cut(s) 518, 542
BcgI CGANNNNNNTGC 2 cut(s) 479, 513
BciVI GTATCC 1 cut(s) 269
BcoDI GTCTC 3 cut(s) 49, 570, 627
BfmI CTRYAG 2 cut(s) 172, 427
BfoI RGCGCY 1 cut(s) 172
BfuI GTATCC 1 cut(s) 269
BisI GCNGC 1 cut(s) 501
BlsI GCNGC 1 cut(s) 502
BmiI GGNNCC 1 cut(s) 447
BmsI GCATC 1 cut(s) 487
Bpu10I CCTNAGC 1 cut(s) 398
Bsa29I ATCGAT 1 cut(s) 522
BsaHI GRCGYC 2 cut(s) 529, 563
BsaJI CCNNGG 2 cut(s) 66, 213
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 95
BseCI ATCGAT 1 cut(s) 522
BseDI CCNNGG 2 cut(s) 66, 213
BseLI CCNNNNNNNGG 2 cut(s) 72, 95
BseRI GAGGAG 1 cut(s) 366
BseXI GCAGC 1 cut(s) 487
Bsh1236I CGCG 1 cut(s) 555
BshNI GGYRCC 1 cut(s) 445
BshVI ATCGAT 1 cut(s) 522
BslFI GGGAC 2 cut(s) 346, 574
BslI CCNNNNNNNGG 2 cut(s) 72, 95
BsmAI GTCTC 3 cut(s) 49, 570, 627
BsmBI CGTCTC 1 cut(s) 570
BsmFI GGGAC 2 cut(s) 346, 574
BsmI GAATGC 1 cut(s) 572
Bsp143I GATC 2 cut(s) 77, 122
BspACI CCGC 2 cut(s) 555, 657
BspDI ATCGAT 1 cut(s) 522
BspFNI CGCG 1 cut(s) 555
BspLI GGNNCC 1 cut(s) 447
BspPI GGATC 1 cut(s) 85
BspT107I GGYRCC 1 cut(s) 445
BssECI CCNNGG 2 cut(s) 66, 213
BssMI GATC 2 cut(s) 77, 122
BssNAI GTATAC 1 cut(s) 589
BssNI GRCGYC 2 cut(s) 529, 563
BssSI CACGAG 1 cut(s) 607
BssT1I CCWWGG 1 cut(s) 66
Bst1107I GTATAC 1 cut(s) 589
Bst2BI CACGAG 1 cut(s) 607
Bst4CI ACNGT 2 cut(s) 281, 439
Bst6I CTCTTC 2 cut(s) 52, 292
BstACI GRCGYC 2 cut(s) 529, 563
BstC8I GCNNGC 2 cut(s) 18, 659
BstDEI CTNAG 1 cut(s) 398
BstDSI CCRYGG 1 cut(s) 213
BstFNI CGCG 1 cut(s) 555
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 2 cut(s) 80, 125
BstMAI GTCTC 3 cut(s) 49, 570, 627
BstMBI GATC 2 cut(s) 77, 122
BstMWI GCNNNNNNNGC 2 cut(s) 26, 380
BstSFI CTRYAG 2 cut(s) 172, 427
BstUI CGCG 1 cut(s) 555
BstV1I GCAGC 1 cut(s) 487
BstX2I RGATCY 1 cut(s) 77
BstYI RGATCY 1 cut(s) 77
BstZ17I GTATAC 1 cut(s) 589
Bsu15I ATCGAT 1 cut(s) 522
BsuI GTATCC 1 cut(s) 269
BsuTUI ATCGAT 1 cut(s) 522
BtgI CCRYGG 1 cut(s) 213
Cac8I GCNNGC 2 cut(s) 18, 659
CfoI GCGC 1 cut(s) 171
ClaI ATCGAT 1 cut(s) 522
CseI GACGC 1 cut(s) 537
Csp6I GTAC 2 cut(s) 255, 446
CviAII CATG 3 cut(s) 83, 380, 569
CviJI RGCY 5 cut(s) 145, 177, 240, 414, 475
CviKI_1 RGCY 5 cut(s) 145, 177, 240, 414, 475
CviQI GTAC 2 cut(s) 255, 446
DdeI CTNAG 1 cut(s) 398
DpnI GATC 2 cut(s) 79, 124
DpnII GATC 2 cut(s) 77, 122
Eam1104I CTCTTC 2 cut(s) 52, 292
EarI CTCTTC 2 cut(s) 52, 292
Eco130I CCWWGG 1 cut(s) 66
Eco47III AGCGCT 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 66
EcoT22I ATGCAT 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 66
Esp3I CGTCTC 1 cut(s) 570
FaeI CATG 3 cut(s) 86, 383, 572
FaqI GGGAC 2 cut(s) 346, 574
FatI CATG 3 cut(s) 82, 379, 568
FauI CCCGC 1 cut(s) 650
FauNDI CATATG 1 cut(s) 313
FblI GTMKAC 3 cut(s) 207, 516, 588
Fnu4HI GCNGC 1 cut(s) 501
Fsp4HI GCNGC 1 cut(s) 501
GlaI GCGC 1 cut(s) 170
GluI GCNGC 1 cut(s) 501
HaeII RGCGCY 1 cut(s) 172
HgaI GACGC 1 cut(s) 537
HhaI GCGC 1 cut(s) 171
Hin1I GRCGYC 2 cut(s) 529, 563
Hin1II CATG 3 cut(s) 86, 383, 572
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HinfI GANTC 3 cut(s) 484, 605, 620
Hpy166II GTNNAC 4 cut(s) 208, 406, 517, 589
Hpy188I TCNGA 2 cut(s) 141, 365
Hpy188III TCNNGA 2 cut(s) 158, 488
Hpy8I GTNNAC 4 cut(s) 208, 406, 517, 589
HpyAV CCTTC 1 cut(s) 505
HpyCH4III ACNGT 2 cut(s) 281, 439
HpyCH4IV ACGT 1 cut(s) 563
HpyCH4V TGCA 3 cut(s) 348, 355, 572
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 380
HpyF3I CTNAG 1 cut(s) 398
HpySE526I ACGT 1 cut(s) 563
Hsp92I GRCGYC 2 cut(s) 529, 563
Hsp92II CATG 3 cut(s) 86, 383, 572
HspAI GCGC 1 cut(s) 169
KpnI GGTACC 1 cut(s) 449
Kzo9I GATC 2 cut(s) 77, 122
LmnI GCTCC 3 cut(s) 25, 365, 379
LpnPI CCDG 9 cut(s) 30, 65, 250, 260, 276, 324, 415, 428, 608
Lsp1109I GCAGC 1 cut(s) 487
LweI GCATC 1 cut(s) 487
MaeII ACGT 1 cut(s) 563
MaeIII GTNAC 1 cut(s) 281
MalI GATC 2 cut(s) 79, 124
MboI GATC 2 cut(s) 77, 122
MboII GAAGA 2 cut(s) 39, 279
MflI RGATCY 1 cut(s) 77
MluCI AATT 2 cut(s) 302, 536
MlyI GAGTC 2 cut(s) 599, 629
MmeI TCCRAC 2 cut(s) 113, 608
MnlI CCTC 7 cut(s) 119, 234, 261, 387, 535, 542, 551
Mph1103I ATGCAT 1 cut(s) 574
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 3 cut(s) 117, 639, 647
MslI CAYNNNNRTG 1 cut(s) 577
Mva1269I GAATGC 1 cut(s) 572
MvnI CGCG 1 cut(s) 555
MwoI GCNNNNNNNGC 2 cut(s) 26, 380
NdeI CATATG 1 cut(s) 313
NdeII GATC 2 cut(s) 77, 122
NlaIII CATG 3 cut(s) 86, 383, 572
NlaIV GGNNCC 1 cut(s) 447
NsiI ATGCAT 1 cut(s) 574
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PctI GAATGC 1 cut(s) 572
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 1 cut(s) 484
PkrI GCNGC 1 cut(s) 502
PleI GAGTC 2 cut(s) 599, 628
PpsI GAGTC 2 cut(s) 599, 628
PspN4I GGNNCC 1 cut(s) 447
PsuI RGATCY 1 cut(s) 77
RsaI GTAC 2 cut(s) 256, 447
RsaNI GTAC 2 cut(s) 255, 446
RseI CAYNNNNRTG 1 cut(s) 577
SaqAI TTAA 3 cut(s) 117, 639, 647
SatI GCNGC 1 cut(s) 501
Sau3AI GATC 2 cut(s) 77, 122
SchI GAGTC 2 cut(s) 599, 629
SetI ASST 6 cut(s) 179, 226, 343, 477, 516, 566
SfaNI GCATC 1 cut(s) 487
SfcI CTRYAG 2 cut(s) 172, 427
SmiMI CAYNNNNRTG 1 cut(s) 577
Sse9I AATT 2 cut(s) 302, 536
SsiI CCGC 2 cut(s) 555, 657
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 2 cut(s) 281, 439
TaiI ACGT 1 cut(s) 566
TaqI TCGA 2 cut(s) 495, 522
TasI AATT 2 cut(s) 302, 536
TfiI GAWTC 1 cut(s) 484
Tru1I TTAA 3 cut(s) 117, 639, 647
Tru9I TTAA 3 cut(s) 117, 639, 647
TseI GCWGC 1 cut(s) 500
TspDTI ATGAA 2 cut(s) 169, 182
TspGWI ACGGA 1 cut(s) 230
XapI RAATTY 1 cut(s) 302
XmiI GTMKAC 3 cut(s) 207, 516, 588
XmnI GAANNNNTTC 1 cut(s) 438
ZraI GACGTC 1 cut(s) 564
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.