MD10G1031000.v1.1

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000532
Physical Location & Seq
Forward (+)
4033006 .. 4034198
1193 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1031000.v1.1.491

Sequence Viewer

Length: 588 bp
ATGAGAGGTTACCTTCTCTCCTTGTTTTTTCTGGGCTCACTGAAAGACATCATTCAGTTATGTGGTGCTGATCCGACTGATGGCTTCGTCGATGTTCCGTTAACGGAATATAATTCCGAATTACAGAAACCGTATGACGTACCACTGGAGGAGCGCTACAGTTTTGTCAATGGTGTAAGGCGTTTGTGGGTCTATGCTGATGACAAGCCCCATGACCCTAATAGCCAAACCCAGCCACGTACTGAAGTTCGCATGAGGGGATTTGACTACTCATCTGGAATATGGCAATTTGAAGGGTATGGATTTGTGCCAAATGGAACCTCTGGTGCTACAGTAGCACAGATCCATGGAGCAGCTAAGGGTGCTACGACTATAATCCTAAGAATCTATAACGGCGACATGAGGTATTATAGTCGAGATTTGGTGGGTACAAATCTTTACGATAAGTGGTTCAGACTTAACATAATCCATGACGTCGACGGAGGGAGCGTGACTATTTTCATTGACGGAGTCCAGAAATTTTCGGGTGAAGGATCAAGGGCCAGGAGATTTGTACTTCAAATGTGGAGTTTATGCTGCACCGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

196

Amino Acids

22.06

Weight (kDa)

5.9

Isoelectric Point (pI)

26.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 40 - 175 6.5e-15 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 477
AccI GTMKAC 1 cut(s) 477
AclWI GGATC 3 cut(s) 65, 337, 541
AcsI RAATTY 1 cut(s) 518
AcuI CTGAAG 1 cut(s) 264
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 4 cut(s) 141, 241, 430, 555
AfeI AGCGCT 1 cut(s) 155
AfiI CCNNNNNNNGG 1 cut(s) 80
AgsI TTSAA 2 cut(s) 293, 560
AjnI CCWGG 1 cut(s) 542
AluBI AGCT 1 cut(s) 356
AluI AGCT 1 cut(s) 356
AlwI GGATC 3 cut(s) 65, 337, 541
Aor51HI AGCGCT 1 cut(s) 155
AoxI GGCC 1 cut(s) 540
ApeKI GCWGC 2 cut(s) 353, 576
ApoI RAATTY 1 cut(s) 518
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 540
AsuHPI GGTGA 1 cut(s) 539
BanII GRGCYC 1 cut(s) 38
BbvI GCAGC 2 cut(s) 365, 563
BccI CCATC 1 cut(s) 74
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 1 cut(s) 544
BfmI CTRYAG 2 cut(s) 157, 330
BfoI RGCGCY 1 cut(s) 157
BisI GCNGC 2 cut(s) 354, 577
BlsI GCNGC 2 cut(s) 355, 578
Bme1390I CCNGG 1 cut(s) 544
BmgT120I GGNCC 1 cut(s) 540
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 1 cut(s) 544
BpmI CTGGAG 1 cut(s) 167
Bpu10I CCTNAGC 1 cut(s) 357
BsaAI YACGTR 1 cut(s) 239
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 1 cut(s) 346
BsaXI ACNNNNNCTCC 3 cut(s) 32, 474, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse118I RCCGGY 1 cut(s) 581
Bse1I ACTGG 1 cut(s) 150
BseBI CCWGG 1 cut(s) 544
BseDI CCNNGG 1 cut(s) 346
BseLI CCNNNNNNNGG 1 cut(s) 80
BseNI ACTGG 1 cut(s) 150
BseRI GAGGAG 1 cut(s) 164
BseXI GCAGC 2 cut(s) 365, 563
BseYI CCCAGC 1 cut(s) 231
BsgI GTGCAG 1 cut(s) 562
BshFI GGCC 1 cut(s) 542
BsiSI CCGG 1 cut(s) 582
BslI CCNNNNNNNGG 1 cut(s) 80
BsnI GGCC 1 cut(s) 542
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 3 cut(s) 70, 342, 533
Bsp19I CCATGG 1 cut(s) 346
BspANI GGCC 1 cut(s) 542
BspLI GGNNCC 1 cut(s) 319
BspPI GGATC 3 cut(s) 65, 337, 541
BsrFI RCCGGY 1 cut(s) 581
BsrI ACTGG 1 cut(s) 150
BssAI RCCGGY 1 cut(s) 581
BssECI CCNNGG 1 cut(s) 346
BssMI GATC 3 cut(s) 70, 342, 533
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 346
Bst2UI CCWGG 1 cut(s) 544
Bst4CI ACNGT 3 cut(s) 132, 161, 334
BstACI GRCGYC 1 cut(s) 474
BstBAI YACGTR 1 cut(s) 239
BstDEI CTNAG 2 cut(s) 357, 380
BstDSI CCRYGG 1 cut(s) 346
BstEII GGTNACC 1 cut(s) 8
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 3 cut(s) 73, 345, 536
BstMBI GATC 3 cut(s) 70, 342, 533
BstMWI GCNNNNNNNGC 3 cut(s) 335, 362, 582
BstNI CCWGG 1 cut(s) 544
BstPI GGTNACC 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 542
BstSFI CTRYAG 2 cut(s) 157, 330
BstV1I GCAGC 2 cut(s) 365, 563
BstX2I RGATCY 1 cut(s) 342
BstYI RGATCY 1 cut(s) 342
BsuRI GGCC 1 cut(s) 542
BtgI CCRYGG 1 cut(s) 346
BtsIMutI CAGTG 2 cut(s) 38, 143
CfoI GCGC 1 cut(s) 156
Cfr10I RCCGGY 1 cut(s) 581
Cfr13I GGNCC 1 cut(s) 540
Csp6I GTAC 4 cut(s) 140, 240, 429, 554
CviAII CATG 5 cut(s) 212, 253, 347, 400, 470
CviJI RGCY 8 cut(s) 36, 84, 208, 225, 235, 356, 542, 585
CviKI_1 RGCY 8 cut(s) 36, 84, 208, 225, 235, 356, 542, 585
CviQI GTAC 4 cut(s) 140, 240, 429, 554
DdeI CTNAG 2 cut(s) 357, 380
DpnI GATC 3 cut(s) 72, 344, 535
DpnII GATC 3 cut(s) 70, 342, 533
Eco130I CCWWGG 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 38
Eco47III AGCGCT 1 cut(s) 155
Eco57I CTGAAG 1 cut(s) 264
Eco91I GGTNACC 1 cut(s) 8
EcoO65I GGTNACC 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 542
EcoT14I CCWWGG 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 346
FaeI CATG 5 cut(s) 215, 256, 350, 403, 473
FatI CATG 5 cut(s) 211, 252, 346, 399, 469
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 2 cut(s) 354, 577
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 2 cut(s) 354, 577
GlaI GCGC 1 cut(s) 155
GluI GCNGC 2 cut(s) 354, 577
GsaI CCCAGC 1 cut(s) 235
GsuI CTGGAG 1 cut(s) 167
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 1 cut(s) 542
HapII CCGG 1 cut(s) 582
HhaI GCGC 1 cut(s) 156
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 5 cut(s) 215, 256, 350, 403, 473
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HincII GTYRAC 2 cut(s) 102, 478
HindII GTYRAC 2 cut(s) 102, 478
HinfI GANTC 2 cut(s) 384, 510
HpaI GTTAAC 1 cut(s) 102
HpaII CCGG 1 cut(s) 582
HphI GGTGA 1 cut(s) 539
Hpy166II GTNNAC 2 cut(s) 102, 478
Hpy188I TCNGA 3 cut(s) 75, 118, 455
Hpy188III TCNNGA 3 cut(s) 276, 416, 514
Hpy8I GTNNAC 2 cut(s) 102, 478
Hpy99I CGWCG 3 cut(s) 92, 479, 482
HpyAV CCTTC 3 cut(s) 23, 287, 524
HpyCH4III ACNGT 3 cut(s) 132, 161, 334
HpyCH4IV ACGT 3 cut(s) 138, 238, 474
HpyCH4V TGCA 1 cut(s) 579
HpyF10VI GCNNNNNNNGC 3 cut(s) 335, 362, 582
HpyF3I CTNAG 2 cut(s) 357, 380
HpySE526I ACGT 3 cut(s) 138, 238, 474
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 5 cut(s) 215, 256, 350, 403, 473
HspAI GCGC 1 cut(s) 154
KspAI GTTAAC 1 cut(s) 102
Kzo9I GATC 3 cut(s) 70, 342, 533
LmnI GCTCC 3 cut(s) 151, 350, 486
LpnPI CCDG 8 cut(s) 17, 131, 245, 261, 309, 527, 529, 556
Lsp1109I GCAGC 2 cut(s) 365, 563
MaeII ACGT 3 cut(s) 138, 238, 474
MaeIII GTNAC 2 cut(s) 8, 490
MalI GATC 3 cut(s) 72, 344, 535
MboI GATC 3 cut(s) 70, 342, 533
MflI RGATCY 1 cut(s) 342
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 4 cut(s) 112, 119, 287, 518
MlyI GAGTC 1 cut(s) 519
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 5 cut(s) 142, 249, 331, 396, 476
MseI TTAA 2 cut(s) 101, 459
MspI CCGG 1 cut(s) 582
MspR9I CCNGG 1 cut(s) 544
MvaI CCWGG 1 cut(s) 544
MwoI GCNNNNNNNGC 3 cut(s) 335, 362, 582
NcoI CCATGG 1 cut(s) 346
NdeII GATC 3 cut(s) 70, 342, 533
NlaIII CATG 5 cut(s) 215, 256, 350, 403, 473
NlaIV GGNNCC 1 cut(s) 319
NmuCI GTSAC 1 cut(s) 490
PcsI WCGNNNNNNNCGW 1 cut(s) 486
PfeI GAWTC 1 cut(s) 384
PflFI GACNNNGTC 1 cut(s) 509
PkrI GCNGC 2 cut(s) 355, 578
PleI GAGTC 1 cut(s) 518
PpsI GAGTC 1 cut(s) 518
Ppu21I YACGTR 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 542
PspEI GGTNACC 1 cut(s) 8
PspFI CCCAGC 1 cut(s) 231
PspGI CCWGG 1 cut(s) 542
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 1 cut(s) 540
PsuI RGATCY 1 cut(s) 342
PsyI GACNNNGTC 1 cut(s) 509
RsaI GTAC 4 cut(s) 141, 241, 430, 555
RsaNI GTAC 4 cut(s) 140, 240, 429, 554
SalI GTCGAC 1 cut(s) 476
SaqAI TTAA 2 cut(s) 101, 459
SatI GCNGC 2 cut(s) 354, 577
Sau3AI GATC 3 cut(s) 70, 342, 533
Sau96I GGNCC 1 cut(s) 540
SchI GAGTC 1 cut(s) 519
ScrFI CCNGG 1 cut(s) 544
SduI GDGCHC 1 cut(s) 38
SetI ASST 8 cut(s) 10, 15, 141, 241, 323, 358, 407, 477
SfcI CTRYAG 2 cut(s) 157, 330
SgrDI CGTCGACG 1 cut(s) 476
Sse9I AATT 4 cut(s) 112, 119, 287, 518
StyD4I CCNGG 1 cut(s) 542
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 3 cut(s) 132, 161, 334
TaiI ACGT 3 cut(s) 141, 241, 477
TaqI TCGA 3 cut(s) 90, 415, 477
TasI AATT 4 cut(s) 112, 119, 287, 518
TatI WGTACW 1 cut(s) 553
TfiI GAWTC 1 cut(s) 384
Tru1I TTAA 2 cut(s) 101, 459
Tru9I TTAA 2 cut(s) 101, 459
TscAI CASTG 2 cut(s) 45, 150
TseFI GTSAC 1 cut(s) 490
TseI GCWGC 2 cut(s) 353, 576
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 1 cut(s) 490
TspGWI ACGGA 4 cut(s) 87, 119, 495, 522
TspRI CASTG 2 cut(s) 45, 150
Tth111I GACNNNGTC 1 cut(s) 509
XapI RAATTY 1 cut(s) 518
XmiI GTMKAC 1 cut(s) 477
ZraI GACGTC 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.