Rroxscaffold_7G00193610

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000532
Physical Location & Seq
Reverse (-)
35129812 .. 35132353
2542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00193610.1

Sequence Viewer

Length: 663 bp
ATGAGAGCTTCCTACTGCTATCTGCTTCTCCTTTTTCTTTTTCCAAGCTCACTGAAATATTTCAGTCAGTTATGTGGTGCTAATCCTGATCCTACCAATGGATTCGTCTCTGTCCCATTATCAGAGTACAACTTTGAATTACAAAAACCCTATGACATACCACTTGAGCAACGATACAGTTATGTTGATGGAGTCAGGCATTTGTGGGTCTATGCCGATGACAAGCCACATAACCCGAATAGTCAAACTCAGCCACGCACTGAAGTTCGCATTAGGGGACTCGACTATTCATCTGGAATATGGCAATTTGAAGGGTATGGCTTTGTGCCAAATGGAACCTCTGGTGCAACCGTAGCACAGATCCATGGAGCAGCTCAGGGCGCCACCACTATTATACTAAGAATCTACAATGGCGATATGAGGTATTATAGTGGAGACTTGGTGGCTACTGATCTTTACGATAAGTGGTTCAGACTTAACATTGTACATGATGTGGATGGAGGGACTGTGACTGTTTATATTGATGGACTCCAGAAATTTCAAGTGAAGGATAAAGGGCCAGGCGATTTGTACTTCAAATGTGGAGTTTATGCTGCTCCACGTAATATAAGCTATTACATGGAATCACGTTGGAAAGACATCAAAATATATAAAAAGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

220

Amino Acids

25.18

Weight (kDa)

7.62

Isoelectric Point (pI)

26.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 44 - 217 1.6e-24 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 380
AclWI GGATC 2 cut(s) 83, 355
AcsI RAATTY 1 cut(s) 536
AcuI CTGAAG 1 cut(s) 282
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 3 cut(s) 128, 486, 572
AfiI CCNNNNNNNGG 1 cut(s) 98
AgsI TTSAA 4 cut(s) 137, 311, 542, 577
AjnI CCWGG 1 cut(s) 559
AluBI AGCT 4 cut(s) 8, 48, 374, 612
AluI AGCT 4 cut(s) 8, 48, 374, 612
Alw26I GTCTC 2 cut(s) 112, 429
AlwI GGATC 2 cut(s) 83, 355
AoxI GGCC 1 cut(s) 557
ApeKI GCWGC 2 cut(s) 371, 593
ApoI RAATTY 1 cut(s) 536
Asp700I GAANNNNTTC 1 cut(s) 59
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 1 cut(s) 557
BanI GGYRCC 1 cut(s) 380
BbvI GCAGC 2 cut(s) 383, 580
BccI CCATC 3 cut(s) 182, 491, 518
BciT130I CCWGG 1 cut(s) 561
BcoDI GTCTC 2 cut(s) 112, 429
BfoI RGCGCY 1 cut(s) 384
BisI GCNGC 2 cut(s) 372, 594
BlsI GCNGC 2 cut(s) 373, 595
Bme1390I CCNGG 1 cut(s) 561
BmgT120I GGNCC 1 cut(s) 557
BmiI GGNNCC 2 cut(s) 337, 382
BmrFI CCNGG 1 cut(s) 561
BpmI CTGGAG 1 cut(s) 515
Bpu10I CCTNAGC 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 185
BsaAI YACGTR 1 cut(s) 602
BsaHI GRCGYC 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 364
BsaXI ACNNNNNCTCC 4 cut(s) 426, 456, 492, 522
Bsc4I CCNNNNNNNGG 1 cut(s) 98
BseBI CCWGG 1 cut(s) 561
BseDI CCNNGG 1 cut(s) 364
BseGI GGATG 1 cut(s) 502
BseLI CCNNNNNNNGG 1 cut(s) 98
BseMII CTCAG 2 cut(s) 263, 389
BseXI GCAGC 2 cut(s) 383, 580
BshFI GGCC 1 cut(s) 559
BshNI GGYRCC 1 cut(s) 380
BslFI GGGAC 3 cut(s) 98, 291, 517
BslI CCNNNNNNNGG 1 cut(s) 98
BsmAI GTCTC 2 cut(s) 112, 429
BsmBI CGTCTC 1 cut(s) 112
BsmFI GGGAC 3 cut(s) 98, 291, 517
BsnI GGCC 1 cut(s) 559
Bsp1407I TGTACA 1 cut(s) 484
Bsp143I GATC 3 cut(s) 88, 360, 451
Bsp19I CCATGG 1 cut(s) 364
BspANI GGCC 1 cut(s) 559
BspCNI CTCAG 2 cut(s) 262, 388
BspLI GGNNCC 2 cut(s) 337, 382
BspPI GGATC 2 cut(s) 83, 355
BspT107I GGYRCC 1 cut(s) 380
BsrGI TGTACA 1 cut(s) 484
BssECI CCNNGG 1 cut(s) 364
BssMI GATC 3 cut(s) 88, 360, 451
BssNI GRCGYC 1 cut(s) 381
BssT1I CCWWGG 1 cut(s) 364
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 4 cut(s) 179, 352, 508, 514
BstACI GRCGYC 1 cut(s) 381
BstAUI TGTACA 1 cut(s) 484
BstBAI YACGTR 1 cut(s) 602
BstDEI CTNAG 3 cut(s) 249, 375, 398
BstDSI CCRYGG 1 cut(s) 364
BstF5I GGATG 1 cut(s) 502
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 3 cut(s) 91, 363, 454
BstMAI GTCTC 2 cut(s) 112, 429
BstMBI GATC 3 cut(s) 88, 360, 451
BstMWI GCNNNNNNNGC 2 cut(s) 353, 380
BstNI CCWGG 1 cut(s) 561
BstSCI CCNGG 1 cut(s) 559
BstV1I GCAGC 2 cut(s) 383, 580
BstX2I RGATCY 1 cut(s) 360
BstYI RGATCY 1 cut(s) 360
BsuRI GGCC 1 cut(s) 559
BtgI CCRYGG 1 cut(s) 364
BtsCI GGATG 1 cut(s) 502
BtsIMutI CAGTG 2 cut(s) 50, 258
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 557
Csp6I GTAC 3 cut(s) 127, 485, 571
CviAII CATG 3 cut(s) 365, 488, 619
CviJI RGCY 9 cut(s) 8, 48, 226, 253, 321, 374, 446, 559, 612
CviKI_1 RGCY 9 cut(s) 8, 48, 226, 253, 321, 374, 446, 559, 612
CviQI GTAC 3 cut(s) 127, 485, 571
DdeI CTNAG 3 cut(s) 249, 375, 398
DinI GGCGCC 1 cut(s) 382
DpnI GATC 3 cut(s) 90, 362, 453
DpnII GATC 3 cut(s) 88, 360, 451
Eco130I CCWWGG 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 282
EcoRII CCWGG 1 cut(s) 559
EcoT14I CCWWGG 1 cut(s) 364
EgeI GGCGCC 1 cut(s) 382
EheI GGCGCC 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 364
Esp3I CGTCTC 1 cut(s) 112
FaeI CATG 3 cut(s) 368, 491, 622
FaqI GGGAC 3 cut(s) 98, 291, 517
FatI CATG 3 cut(s) 364, 487, 618
Fnu4HI GCNGC 2 cut(s) 372, 594
FokI GGATG 1 cut(s) 509
Fsp4HI GCNGC 2 cut(s) 372, 594
GlaI GCGC 1 cut(s) 382
GluI GCNGC 2 cut(s) 372, 594
GsuI CTGGAG 1 cut(s) 515
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 1 cut(s) 559
HhaI GCGC 1 cut(s) 383
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 3 cut(s) 368, 491, 622
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HinfI GANTC 6 cut(s) 102, 192, 279, 402, 528, 623
Hpy188I TCNGA 2 cut(s) 124, 473
Hpy188III TCNNGA 3 cut(s) 86, 294, 532
HpyAV CCTTC 2 cut(s) 305, 541
HpyCH4III ACNGT 4 cut(s) 179, 352, 508, 514
HpyCH4IV ACGT 2 cut(s) 601, 628
HpyCH4V TGCA 1 cut(s) 347
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 380
HpyF3I CTNAG 3 cut(s) 249, 375, 398
HpySE526I ACGT 2 cut(s) 601, 628
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 3 cut(s) 368, 491, 622
HspAI GCGC 1 cut(s) 381
KasI GGCGCC 1 cut(s) 380
Kzo9I GATC 3 cut(s) 88, 360, 451
LmnI GCTCC 2 cut(s) 368, 601
LpnPI CCDG 8 cut(s) 99, 181, 279, 327, 362, 545, 546, 573
Lsp1109I GCAGC 2 cut(s) 383, 580
MaeII ACGT 2 cut(s) 601, 628
MaeIII GTNAC 1 cut(s) 508
MalI GATC 3 cut(s) 90, 362, 453
MboI GATC 3 cut(s) 88, 360, 451
MflI RGATCY 1 cut(s) 360
MluCI AATT 3 cut(s) 137, 305, 536
Mly113I GGCGCC 1 cut(s) 381
MlyI GAGTC 3 cut(s) 201, 273, 522
MmeI TCCRAC 1 cut(s) 611
MnlI CCTC 3 cut(s) 349, 414, 494
MroXI GAANNNNTTC 1 cut(s) 59
MseI TTAA 1 cut(s) 477
MspR9I CCNGG 1 cut(s) 561
MvaI CCWGG 1 cut(s) 561
MwoI GCNNNNNNNGC 2 cut(s) 353, 380
NarI GGCGCC 1 cut(s) 381
NcoI CCATGG 1 cut(s) 364
NdeII GATC 3 cut(s) 88, 360, 451
NlaIII CATG 3 cut(s) 368, 491, 622
NlaIV GGNNCC 2 cut(s) 337, 382
NmuCI GTSAC 1 cut(s) 508
PdmI GAANNNNTTC 1 cut(s) 59
PfeI GAWTC 3 cut(s) 102, 402, 623
PkrI GCNGC 2 cut(s) 373, 595
PleI GAGTC 3 cut(s) 200, 273, 522
PluTI GGCGCC 1 cut(s) 384
PpsI GAGTC 3 cut(s) 200, 273, 522
Ppu21I YACGTR 1 cut(s) 602
Psp6I CCWGG 1 cut(s) 559
PspGI CCWGG 1 cut(s) 559
PspN4I GGNNCC 2 cut(s) 337, 382
PspPI GGNCC 1 cut(s) 557
PsuI RGATCY 1 cut(s) 360
RsaI GTAC 3 cut(s) 128, 486, 572
RsaNI GTAC 3 cut(s) 127, 485, 571
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 2 cut(s) 372, 594
Sau3AI GATC 3 cut(s) 88, 360, 451
Sau96I GGNCC 1 cut(s) 557
SchI GAGTC 3 cut(s) 201, 273, 522
ScrFI CCNGG 1 cut(s) 561
SetI ASST 8 cut(s) 10, 50, 341, 376, 425, 604, 614, 631
SfoI GGCGCC 1 cut(s) 382
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 3 cut(s) 137, 305, 536
SspDI GGCGCC 1 cut(s) 380
SspI AATATT 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 559
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 4 cut(s) 179, 352, 508, 514
TaiI ACGT 2 cut(s) 604, 631
TaqI TCGA 1 cut(s) 282
TasI AATT 3 cut(s) 137, 305, 536
TatI WGTACW 3 cut(s) 126, 484, 570
TfiI GAWTC 3 cut(s) 102, 402, 623
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 2 cut(s) 57, 265
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 2 cut(s) 371, 593
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 1 cut(s) 279
TspRI CASTG 2 cut(s) 57, 265
XapI RAATTY 1 cut(s) 536
XmnI GAANNNNTTC 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.