FvH4_2g12340

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000458
Physical Location & Seq
Reverse (-)
10818083 .. 10824960
6878 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g12340.t1

Sequence Viewer

Length: 1359 bp
ATGGGTGGGATTCGCAACAAGGTGAAACGGACGCCCAAAGTAGAAGATATCACTGTAGCTATAAATTACCATCATGCTAGTAATGGCCCAAGAGCATATGATCATAATCCTAATTGGTTTCCATTTACAAATGGACATCCAATTGATATCAATGATGGAGCTGCTTGCTTCTCTAAACAGGCCGCTGTTGCTAAAGTTTCAGCAGTAAGCTCTCTTTTAGGTAAAGCTAGCTCGACTGGGTTTGGAAAGGCAATGGATATCTTGGACACGTTCGGCAGTAGCATCAGTAATTGGGACCCCACTTTATATTCTGAGGTGCAAACAAAAGGGAATACAATTGCAATTTTGGCTTTTGAAGTTGCAAACTCAATTGTCAAGGGTGTGAAGCTAATGCAGTCCCTGTCAGAGGATAATATCAGATATTTAAAAGAGGTGCTGCTCCCATCCAAAGGGGTGCAAAGTTTAATATCTACTGATATGGATGAGCTCCTCAGAATTGCCGCTGCTGACAAGAGAGAAGAATTGAGAATTTTTATCAGAGATGTTGTACGTTTTGGAAATCGCACTTATGATCCAATATGGCACAACTTGGATCGCTATTTTAGCAAGATTGGCTCGAAGGTTACTGAACAAACTCATTTGAAGAAGCAAGCAGAGAGAGGGATGGAATATTTGATAAATTTAGTTCAGTCAACCGCGGAACTGTGTCATGAGTTGCATTCGTTGGATAGATTGGAACAAGAATATCGGCATAAGCTTGAAGAAGATAGTTCAGTTACTGCAAGAAGAGGAGACAACCTTGCGATTTTAAAAGCAGATATAAAGAATCAAATGAAGCTTGTAAACAGTTTAAAGAAAAATTCACTTTGGTCTAAGACTTTGGAAGAGGTTATGCCGAAGCTTGTTGACATTGTACAATTATTACACTTAAAGATTCATGAAGCTTTTGGAAGTCCTGATGATGATGAACCAGTGAAACGTACTCAGAGTAACTGTAAAAGGTTGGGGCCTACTGGACCTTCCTTACACTACGCACATATTATTACGCAAATTAACACACTTGTGTCTCGATCAAGTTTAGTGCCTCAAAGTGTGATAGATACTCTGTATGAGGGACTACCATGCAGTGTCAAGTCAGCTCTGCGCTCAAAATTTCAGTTAGTTGAGCTCACCATTCCTCAAATCAAAGCTGAAATGAAGCGAACTTTGGATTGGCTTGTTCCCATTGCCACTAACACAACAAAAGCTCTTCATGGCTTTGGATGGGTTGGAGAATGGGCAAATGCAAGGTTTGAGGCGAGCTGGCGCACTGGCTTGACTGATACTCTGAGGATTGAGACTCTACACCATTGCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

453

Amino Acids

50.79

Weight (kDa)

8.74

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 115 - 171 4.8e-23 Domain of unknown function (DUF3475)
DUF668 PF05003 334 - 415 6.2e-22 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34320 AT1G34320
fragaria_vesca FvH4_2g12340 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_5g36070 FvH4_5g36100 FvH4_5g36130 FvH4_5g36140
malus_domestica MD05G1039900.v1.1 MD10G1045400.v1.1 MD10G1045600.v1.1 MD10G1045700.v1.1 MD10G1045800.v1.1 MD12G1057900.v1.1
prunus_persica Prupe.1G525500_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1
pyrus_communis pycom05g03250 pycom10g03180 pycom10g03190 pycom15g33960
rosa_chinensis RchiOBHm_Chr3g0484671 RchiOBHm_Chr6g0272351 RchiOBHm_Chr6g0272361 RchiOBHm_Chr7g0237881
rosa_laevigata RLG00000000952 RLG00000000960 RLG00000001025 RLG00000008689 RLG00000010609 RLG00000013679 RLG00000013680
rosa_multiflora Rmu_sc0001339.1_g000015 Rmu_sc0002877.1_g000020 Rmu_sc0009379.1_g000003 Rmu_ssc0000199.1_g000001 Rmu_ssc0000321.1_g000001
rosa_roxburghii Rroxscaffold_1G00039160 Rroxscaffold_3G00223500 Rroxscaffold_3G00223670 Rroxscaffold_7G00196370 Rroxscaffold_7G00196380
rosa_rugosa Rorug06G0068200 Rorug06G0068300 Rorug06G0068400 Rorug07G0302100 Rorug07G0308600 Rorug07G0309200.1
rosa_samantha Rh3AG263300 Rh3DG292000 Rh6AG113700 Rh6AG184400 Rh6AG184500 Rh6BG187800 Rh6BG187900 Rh6CG185900 Rh6DG177600 Rh6DG177700 Rh7AG458500 Rh7AG464200 Rh7AG464500 Rh7BG429200 Rh7BG434200 Rh7CG476400 Rh7CG481600 Rh7CG482700 Rh7DG445700 Rh7DG450300 Rh7DG451400
rosa_wichuraiana Rw0G019450 Rw6G015900 Rw6G015910 Rw6G015940 Rw6G015950 Rw7G037980 Rw7G038410 Rw7G038470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 698
AciI CCGC 4 cut(s) 183, 501, 696, 698
AclWI GGATC 2 cut(s) 566, 600
AcsI RAATTY 4 cut(s) 528, 679, 859, 1151
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 3 cut(s) 549, 915, 982
AfiI CCNNNNNNNGG 2 cut(s) 406, 449
AflIII ACRYGT 1 cut(s) 267
AgsI TTSAA 3 cut(s) 356, 643, 761
AjuI GAANNNNNNNTTGG 4 cut(s) 1190, 1195, 1222, 1227
AleI CACNNNNGTG 1 cut(s) 1061
Alw21I GWGCWC 2 cut(s) 489, 1170
Alw26I GTCTC 3 cut(s) 786, 1071, 1331
AlwI GGATC 2 cut(s) 566, 600
AlwNI CAGNNNCTG 2 cut(s) 400, 779
AoxI GGCC 3 cut(s) 85, 180, 1007
ApeKI GCWGC 3 cut(s) 161, 436, 503
ApoI RAATTY 4 cut(s) 528, 679, 859, 1151
AspLEI GCGC 2 cut(s) 1146, 1308
AspS9I GGNCC 4 cut(s) 86, 295, 1007, 1016
AsuHPI GGTGA 2 cut(s) 34, 1162
AsuNHI GCTAGC 1 cut(s) 227
AvaII GGWCC 2 cut(s) 295, 1016
BanII GRGCYC 2 cut(s) 489, 1170
Bbv12I GWGCWC 2 cut(s) 489, 1170
BbvI GCAGC 3 cut(s) 148, 423, 490
BccI CCATC 5 cut(s) 78, 149, 451, 658, 1257
BclI TGATCA 1 cut(s) 100
BcoDI GTCTC 3 cut(s) 786, 1071, 1331
BfaI CTAG 2 cut(s) 78, 228
BfmI CTRYAG 1 cut(s) 54
BisI GCNGC 5 cut(s) 162, 183, 437, 501, 504
BlsI GCNGC 5 cut(s) 163, 184, 438, 502, 505
Bme18I GGWCC 2 cut(s) 295, 1016
BmgT120I GGNCC 4 cut(s) 86, 295, 1007, 1016
BmiI GGNNCC 3 cut(s) 296, 297, 1008
BmrI ACTGGG 1 cut(s) 246
BmsI GCATC 1 cut(s) 291
BmtI GCTAGC 1 cut(s) 231
BmuI ACTGGG 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 696
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 449
Bse1I ACTGG 4 cut(s) 241, 971, 1018, 1315
Bse3DI GCAATG 3 cut(s) 258, 1224, 1348
Bse8I GATNNNNATC 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 696
BseGI GGATG 5 cut(s) 136, 443, 487, 669, 1268
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 2 cut(s) 406, 449
BseMI GCAATG 3 cut(s) 258, 1224, 1348
BseMII CTCAG 4 cut(s) 303, 505, 998, 1319
BseNI ACTGG 4 cut(s) 241, 971, 1018, 1315
BseRI GAGGAG 2 cut(s) 479, 804
BseXI GCAGC 3 cut(s) 148, 423, 490
Bsh1236I CGCG 1 cut(s) 698
BshFI GGCC 3 cut(s) 87, 182, 1009
BsiHKAI GWGCWC 2 cut(s) 489, 1170
BslFI GGGAC 3 cut(s) 308, 382, 1128
BslI CCNNNNNNNGG 2 cut(s) 406, 449
BsmAI GTCTC 3 cut(s) 786, 1071, 1331
BsmFI GGGAC 3 cut(s) 308, 382, 1128
BsmI GAATGC 1 cut(s) 718
BsnI GGCC 3 cut(s) 87, 182, 1009
Bsp1286I GDGCHC 2 cut(s) 489, 1170
Bsp1407I TGTACA 1 cut(s) 913
Bsp143I GATC 4 cut(s) 100, 571, 592, 1070
BspACI CCGC 4 cut(s) 183, 501, 696, 698
BspANI GGCC 3 cut(s) 87, 182, 1009
BspCNI CTCAG 4 cut(s) 304, 504, 997, 1320
BspFNI CGCG 1 cut(s) 698
BspHI TCATGA 2 cut(s) 709, 937
BspLI GGNNCC 3 cut(s) 296, 297, 1008
BspOI GCTAGC 1 cut(s) 231
BspPI GGATC 2 cut(s) 566, 600
BspQI GCTCTTC 1 cut(s) 1254
BsrDI GCAATG 3 cut(s) 258, 1224, 1348
BsrGI TGTACA 1 cut(s) 913
BsrI ACTGG 4 cut(s) 241, 971, 1018, 1315
BssECI CCNNGG 1 cut(s) 696
BssMI GATC 4 cut(s) 100, 571, 592, 1070
BssNI GRCGYC 1 cut(s) 32
Bst4CI ACNGT 4 cut(s) 55, 705, 848, 995
Bst6I CTCTTC 3 cut(s) 781, 879, 1254
BstACI GRCGYC 1 cut(s) 32
BstAUI TGTACA 1 cut(s) 913
BstC8I GCNNGC 5 cut(s) 166, 229, 651, 1300, 1304
BstDEI CTNAG 5 cut(s) 312, 491, 873, 984, 1328
BstDSI CCRYGG 1 cut(s) 696
BstENI CCTNNNNNAGG 1 cut(s) 404
BstF5I GGATG 5 cut(s) 136, 443, 487, 669, 1268
BstFNI CGCG 1 cut(s) 698
BstHHI GCGC 2 cut(s) 1146, 1308
BstKTI GATC 4 cut(s) 103, 574, 595, 1073
BstMAI GTCTC 3 cut(s) 786, 1071, 1331
BstMBI GATC 4 cut(s) 100, 571, 592, 1070
BstMWI GCNNNNNNNGC 4 cut(s) 188, 347, 603, 612
BstSFI CTRYAG 1 cut(s) 54
BstUI CGCG 1 cut(s) 698
BstV1I GCAGC 3 cut(s) 148, 423, 490
BsuRI GGCC 3 cut(s) 87, 182, 1009
BtgI CCRYGG 1 cut(s) 696
BtsCI GGATG 5 cut(s) 136, 443, 487, 669, 1268
BtsI GCAGTG 1 cut(s) 1132
BtsIMutI CAGTG 4 cut(s) 51, 978, 1132, 1308
Cac8I GCNNGC 5 cut(s) 166, 229, 651, 1300, 1304
CaiI CAGNNNCTG 2 cut(s) 400, 779
CciI TCATGA 2 cut(s) 709, 937
CfoI GCGC 2 cut(s) 1146, 1308
Cfr13I GGNCC 4 cut(s) 86, 295, 1007, 1016
Cfr42I CCGCGG 1 cut(s) 699
CseI GACGC 1 cut(s) 40
Csp6I GTAC 3 cut(s) 548, 914, 981
CviAII CATG 5 cut(s) 74, 710, 938, 1122, 1253
CviQI GTAC 3 cut(s) 548, 914, 981
DdeI CTNAG 5 cut(s) 312, 491, 873, 984, 1328
DpnI GATC 4 cut(s) 102, 573, 594, 1072
DpnII GATC 4 cut(s) 100, 571, 592, 1070
DraI TTTAAA 3 cut(s) 426, 810, 852
Eam1104I CTCTTC 3 cut(s) 781, 879, 1254
EarI CTCTTC 3 cut(s) 781, 879, 1254
Ecl136II GAGCTC 2 cut(s) 487, 1168
Eco24I GRGCYC 2 cut(s) 489, 1170
Eco32I GATATC 3 cut(s) 49, 148, 259
Eco47I GGWCC 2 cut(s) 295, 1016
Eco53kI GAGCTC 2 cut(s) 487, 1168
EcoICRI GAGCTC 2 cut(s) 487, 1168
EcoNI CCTNNNNNAGG 1 cut(s) 404
EcoO109I RGGNCCY 2 cut(s) 295, 1007
EcoRV GATATC 3 cut(s) 49, 148, 259
EcoT38I GRGCYC 2 cut(s) 489, 1170
FaeI CATG 5 cut(s) 77, 713, 941, 1125, 1256
FaqI GGGAC 3 cut(s) 308, 382, 1128
FatI CATG 5 cut(s) 73, 709, 937, 1121, 1252
FauNDI CATATG 1 cut(s) 97
FbaI TGATCA 1 cut(s) 100
Fnu4HI GCNGC 5 cut(s) 162, 183, 437, 501, 504
FokI GGATG 5 cut(s) 123, 430, 494, 676, 1275
FriOI GRGCYC 2 cut(s) 489, 1170
Fsp4HI GCNGC 5 cut(s) 162, 183, 437, 501, 504
FspBI CTAG 2 cut(s) 78, 228
GlaI GCGC 2 cut(s) 1145, 1307
GluI GCNGC 5 cut(s) 162, 183, 437, 501, 504
HaeIII GGCC 3 cut(s) 87, 182, 1009
HgaI GACGC 1 cut(s) 40
HhaI GCGC 2 cut(s) 1146, 1308
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 5 cut(s) 77, 713, 941, 1125, 1256
Hin6I GCGC 2 cut(s) 1144, 1306
HinP1I GCGC 2 cut(s) 1144, 1306
HincII GTYRAC 2 cut(s) 693, 907
HindII GTYRAC 2 cut(s) 693, 907
HindIII AAGCTT 4 cut(s) 755, 836, 899, 942
HinfI GANTC 4 cut(s) 10, 826, 934, 1339
HphI GGTGA 2 cut(s) 34, 1162
Hpy166II GTNNAC 3 cut(s) 693, 844, 907
Hpy188I TCNGA 7 cut(s) 313, 406, 419, 494, 539, 987, 1329
Hpy188III TCNNGA 4 cut(s) 710, 938, 956, 1068
Hpy8I GTNNAC 3 cut(s) 693, 844, 907
HpyAV CCTTC 2 cut(s) 613, 1029
HpyCH4III ACNGT 4 cut(s) 55, 705, 848, 995
HpyCH4IV ACGT 3 cut(s) 269, 550, 979
HpyCH4V TGCA 9 cut(s) 319, 341, 362, 394, 457, 718, 782, 1125, 1286
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 347, 603, 612
HpyF3I CTNAG 5 cut(s) 312, 491, 873, 984, 1328
HpySE526I ACGT 3 cut(s) 269, 550, 979
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 5 cut(s) 77, 713, 941, 1125, 1256
HspAI GCGC 2 cut(s) 1144, 1306
KflI GGGWCCC 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 100
KspI CCGCGG 1 cut(s) 699
Kzo9I GATC 4 cut(s) 100, 571, 592, 1070
LguI GCTCTTC 1 cut(s) 1254
LmnI GCTCC 3 cut(s) 158, 444, 492
LpnPI CCDG 8 cut(s) 164, 222, 413, 969, 984, 999, 1288, 1296
Lsp1109I GCAGC 3 cut(s) 148, 423, 490
LweI GCATC 1 cut(s) 291
MaeI CTAG 2 cut(s) 78, 228
MaeII ACGT 3 cut(s) 269, 550, 979
MaeIII GTNAC 3 cut(s) 622, 775, 989
MalI GATC 4 cut(s) 102, 573, 594, 1072
MboI GATC 4 cut(s) 100, 571, 592, 1070
MboII GAAGA 8 cut(s) 56, 530, 655, 773, 776, 798, 896, 1241
MfeI CAATTG 3 cut(s) 141, 336, 369
MhlI GDGCHC 2 cut(s) 489, 1170
MlyI GAGTC 1 cut(s) 1333
MmeI TCCRAC 2 cut(s) 705, 1249
MseI TTAA 6 cut(s) 425, 464, 809, 851, 929, 1053
MslI CAYNNNNRTG 1 cut(s) 1061
MspA1I CMGCKG 3 cut(s) 185, 503, 698
MunI CAATTG 3 cut(s) 141, 336, 369
Mva1269I GAATGC 1 cut(s) 718
MvnI CGCG 1 cut(s) 698
MwoI GCNNNNNNNGC 4 cut(s) 188, 347, 603, 612
NdeI CATATG 1 cut(s) 97
NdeII GATC 4 cut(s) 100, 571, 592, 1070
NheI GCTAGC 1 cut(s) 227
NlaIII CATG 5 cut(s) 77, 713, 941, 1125, 1256
NlaIV GGNNCC 3 cut(s) 296, 297, 1008
OliI CACNNNNGTG 1 cut(s) 1061
PagI TCATGA 2 cut(s) 709, 937
PciSI GCTCTTC 1 cut(s) 1254
PctI GAATGC 1 cut(s) 718
PfeI GAWTC 3 cut(s) 10, 826, 934
PkrI GCNGC 5 cut(s) 163, 184, 438, 502, 505
PleI GAGTC 1 cut(s) 1333
PpsI GAGTC 1 cut(s) 1333
PpuMI RGGWCCY 1 cut(s) 295
Psp124BI GAGCTC 2 cut(s) 489, 1170
Psp5II RGGWCCY 1 cut(s) 295
PspN4I GGNNCC 3 cut(s) 296, 297, 1008
PspPI GGNCC 4 cut(s) 86, 295, 1007, 1016
PspPPI RGGWCCY 1 cut(s) 295
PstNI CAGNNNCTG 2 cut(s) 400, 779
RsaI GTAC 3 cut(s) 549, 915, 982
RsaNI GTAC 3 cut(s) 548, 914, 981
RseI CAYNNNNRTG 1 cut(s) 1061
SacI GAGCTC 2 cut(s) 489, 1170
SacII CCGCGG 1 cut(s) 699
SapI GCTCTTC 1 cut(s) 1254
SaqAI TTAA 6 cut(s) 425, 464, 809, 851, 929, 1053
SatI GCNGC 5 cut(s) 162, 183, 437, 501, 504
Sau3AI GATC 4 cut(s) 100, 571, 592, 1070
Sau96I GGNCC 4 cut(s) 86, 295, 1007, 1016
SchI GAGTC 1 cut(s) 1333
SduI GDGCHC 2 cut(s) 489, 1170
SfaNI GCATC 1 cut(s) 291
SfcI CTRYAG 1 cut(s) 54
Sfr303I CCGCGG 1 cut(s) 699
SgrBI CCGCGG 1 cut(s) 699
SinI GGWCC 2 cut(s) 295, 1016
SmiMI CAYNNNNRTG 1 cut(s) 1061
SsiI CCGC 4 cut(s) 183, 501, 696, 698
SspI AATATT 1 cut(s) 671
SspMI CTAG 2 cut(s) 78, 228
SstI GAGCTC 2 cut(s) 489, 1170
TaaI ACNGT 4 cut(s) 55, 705, 848, 995
TaiI ACGT 3 cut(s) 272, 553, 982
TaqI TCGA 3 cut(s) 233, 617, 1069
TatI WGTACW 1 cut(s) 913
TauI GCSGC 2 cut(s) 185, 503
TfiI GAWTC 3 cut(s) 10, 826, 934
Tru1I TTAA 6 cut(s) 425, 464, 809, 851, 929, 1053
Tru9I TTAA 6 cut(s) 425, 464, 809, 851, 929, 1053
TscAI CASTG 4 cut(s) 58, 978, 1132, 1315
TseI GCWGC 3 cut(s) 161, 436, 503
TspDTI ATGAA 6 cut(s) 848, 926, 954, 981, 1211, 1241
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 4 cut(s) 58, 978, 1132, 1315
VpaK11BI GGWCC 2 cut(s) 295, 1016
XagI CCTNNNNNAGG 1 cut(s) 404
XapI RAATTY 4 cut(s) 528, 679, 859, 1151
XspI CTAG 2 cut(s) 78, 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.