Rh3AG263300

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0000458
Physical Location & Seq
Reverse (-)
29320483 .. 29321052
570 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG263300.1

Sequence Viewer

Length: 570 bp
ATGTTTGGGTTTCTTAGTCTACCCTTCCTTTTATGTAGTTCTGAGACAAACCGTAAACCAACTCCACAGACTGAGGTGATTCGGATTGAGACATTCCATCATGCAAATAGGGAAAAAAGAGAAGCCTGTGTACTCAAACTAGTATTGTGGCTTCATTATTTGGTCTCTCAAAGTAAGCGTGCATCCACTAGTGTGGGTATGCGGTCACCTGTTAAGTCCATAGCTACCAAAACCCTCCCAAAGACAAATGAGCAGCTCAAGGACATTCCCCCCTCAACCATGCTAACAACTGACGACCAGAGGATGCTGCAGGATGTCAGTAAGAGAAGCCGGGTTCCCGGAATTAGTAAAAGTCAGGACTATGACAACGCAAACTCTAGTTTGGGAAACCAGAGCAGATTGAACAAGAGTAACAGCTATACATCTGGGGAAGAAACCAAGAAATTGTTGCCTTTCAGTAGGCTCTCTTCTGGGGTTCCTGTCTTTGATTATGGTATAGATAAGGAAAAAGCCTTGGATGTCATAGATAGAGTGGACACACAGAGAGATCATCATCATCCAAGGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

189

Amino Acids

21.46

Weight (kDa)

9.49

Isoelectric Point (pI)

56.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34320 AT1G34320
fragaria_vesca FvH4_2g12340 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_5g36070 FvH4_5g36100 FvH4_5g36130 FvH4_5g36140
malus_domestica MD05G1039900.v1.1 MD10G1045400.v1.1 MD10G1045600.v1.1 MD10G1045700.v1.1 MD10G1045800.v1.1 MD12G1057900.v1.1
prunus_persica Prupe.1G525500_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1
pyrus_communis pycom05g03250 pycom10g03180 pycom10g03190 pycom15g33960
rosa_chinensis RchiOBHm_Chr3g0484671 RchiOBHm_Chr6g0272351 RchiOBHm_Chr6g0272361 RchiOBHm_Chr7g0237881
rosa_laevigata RLG00000000952 RLG00000000960 RLG00000001025 RLG00000008689 RLG00000010609 RLG00000013679 RLG00000013680
rosa_multiflora Rmu_sc0001339.1_g000015 Rmu_sc0002877.1_g000020 Rmu_sc0009379.1_g000003 Rmu_ssc0000199.1_g000001 Rmu_ssc0000321.1_g000001
rosa_roxburghii Rroxscaffold_1G00039160 Rroxscaffold_3G00223500 Rroxscaffold_3G00223670 Rroxscaffold_7G00196370 Rroxscaffold_7G00196380
rosa_rugosa Rorug06G0068200 Rorug06G0068300 Rorug06G0068400 Rorug07G0302100 Rorug07G0308600 Rorug07G0309200.1
rosa_samantha Rh3AG263300 Rh3DG292000 Rh6AG113700 Rh6AG184400 Rh6AG184500 Rh6BG187800 Rh6BG187900 Rh6CG185900 Rh6DG177600 Rh6DG177700 Rh7AG458500 Rh7AG464200 Rh7AG464500 Rh7BG429200 Rh7BG434200 Rh7CG476400 Rh7CG481600 Rh7CG482700 Rh7DG445700 Rh7DG450300 Rh7DG451400
rosa_wichuraiana Rw0G019450 Rw6G015900 Rw6G015910 Rw6G015940 Rw6G015950 Rw7G037980 Rw7G038410 Rw7G038470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 19
AciI CCGC 1 cut(s) 202
AfaI GTAC 1 cut(s) 132
AgsI TTSAA 1 cut(s) 403
AhlI ACTAGT 2 cut(s) 139, 188
AleI CACNNNNGTG 1 cut(s) 191
AluBI AGCT 3 cut(s) 224, 256, 417
AluI AGCT 3 cut(s) 224, 256, 417
Alw26I GTCTC 3 cut(s) 38, 83, 169
ApeKI GCWGC 2 cut(s) 253, 307
AsuC2I CCSGG 2 cut(s) 332, 339
AsuHPI GGTGA 2 cut(s) 88, 198
BarI GAAGNNNNNNTAC 6 cut(s) 114, 135, 146, 167, 451, 483
BbvI GCAGC 2 cut(s) 265, 294
BccI CCATC 1 cut(s) 105
BcnI CCSGG 2 cut(s) 332, 339
BcoDI GTCTC 3 cut(s) 38, 83, 169
BcuI ACTAGT 2 cut(s) 139, 188
BfaI CTAG 3 cut(s) 140, 189, 378
BfmI CTRYAG 1 cut(s) 308
BisI GCNGC 2 cut(s) 254, 308
BlsI GCNGC 2 cut(s) 255, 309
Bme1390I CCNGG 2 cut(s) 332, 339
BmiI GGNNCC 2 cut(s) 336, 477
BmrFI CCNGG 2 cut(s) 332, 339
BmsI GCATC 2 cut(s) 191, 294
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 2 cut(s) 332, 339
BsaBI GATNNNNATC 1 cut(s) 552
BsaI GGTCTC 1 cut(s) 169
BsaJI CCNNGG 2 cut(s) 513, 560
Bse8I GATNNNNATC 1 cut(s) 552
BseDI CCNNGG 2 cut(s) 513, 560
BseGI GGATG 5 cut(s) 182, 309, 319, 523, 556
BseJI GATNNNNATC 1 cut(s) 552
BseMII CTCAG 2 cut(s) 33, 63
BseXI GCAGC 2 cut(s) 265, 294
BsiSI CCGG 2 cut(s) 331, 339
BsmAI GTCTC 3 cut(s) 38, 83, 169
Bso31I GGTCTC 1 cut(s) 169
Bsp143I GATC 1 cut(s) 547
BspACI CCGC 1 cut(s) 202
BspCNI CTCAG 2 cut(s) 34, 64
BspLI GGNNCC 2 cut(s) 336, 477
BspMAI CTGCAG 1 cut(s) 312
BspTNI GGTCTC 1 cut(s) 169
BssECI CCNNGG 2 cut(s) 513, 560
BssMI GATC 1 cut(s) 547
BssT1I CCWWGG 2 cut(s) 513, 560
Bst4CI ACNGT 1 cut(s) 53
Bst6I CTCTTC 1 cut(s) 472
BstC8I GCNNGC 1 cut(s) 180
BstDEI CTNAG 3 cut(s) 14, 42, 72
BstEII GGTNACC 1 cut(s) 204
BstF5I GGATG 5 cut(s) 182, 309, 319, 523, 556
BstKTI GATC 1 cut(s) 550
BstMAI GTCTC 3 cut(s) 38, 83, 169
BstMBI GATC 1 cut(s) 547
BstPI GGTNACC 1 cut(s) 204
BstSCI CCNGG 2 cut(s) 330, 337
BstSFI CTRYAG 1 cut(s) 308
BstV1I GCAGC 2 cut(s) 265, 294
BstXI CCANNNNNNTGG 1 cut(s) 193
BtsCI GGATG 5 cut(s) 182, 309, 319, 523, 556
Cac8I GCNNGC 1 cut(s) 180
Csp6I GTAC 1 cut(s) 131
CviAII CATG 2 cut(s) 101, 280
CviJI RGCY 8 cut(s) 125, 151, 224, 256, 330, 417, 463, 512
CviKI_1 RGCY 8 cut(s) 125, 151, 224, 256, 330, 417, 463, 512
CviQI GTAC 1 cut(s) 131
DdeI CTNAG 3 cut(s) 14, 42, 72
DpnI GATC 1 cut(s) 549
DpnII GATC 1 cut(s) 547
Eam1104I CTCTTC 1 cut(s) 472
EarI CTCTTC 1 cut(s) 472
Eco130I CCWWGG 2 cut(s) 513, 560
Eco31I GGTCTC 1 cut(s) 169
Eco91I GGTNACC 1 cut(s) 204
EcoO65I GGTNACC 1 cut(s) 204
EcoT14I CCWWGG 2 cut(s) 513, 560
ErhI CCWWGG 2 cut(s) 513, 560
FaeI CATG 2 cut(s) 104, 283
FatI CATG 2 cut(s) 100, 279
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 2 cut(s) 254, 308
FokI GGATG 5 cut(s) 169, 316, 326, 530, 543
Fsp4HI GCNGC 2 cut(s) 254, 308
FspBI CTAG 3 cut(s) 140, 189, 378
GluI GCNGC 2 cut(s) 254, 308
HapII CCGG 2 cut(s) 331, 339
Hin1II CATG 2 cut(s) 104, 283
HinfI GANTC 1 cut(s) 79
HpaII CCGG 2 cut(s) 331, 339
HphI GGTGA 2 cut(s) 88, 198
Hpy166II GTNNAC 4 cut(s) 20, 56, 131, 535
Hpy188I TCNGA 2 cut(s) 43, 84
Hpy188III TCNNGA 1 cut(s) 356
Hpy8I GTNNAC 4 cut(s) 20, 56, 131, 535
HpyAV CCTTC 1 cut(s) 34
HpyCH4III ACNGT 1 cut(s) 53
HpyCH4V TGCA 3 cut(s) 104, 182, 310
HpyF3I CTNAG 3 cut(s) 14, 42, 72
Hsp92II CATG 2 cut(s) 104, 283
Kzo9I GATC 1 cut(s) 547
Lsp1109I GCAGC 2 cut(s) 265, 294
LweI GCATC 2 cut(s) 191, 294
MaeI CTAG 3 cut(s) 140, 189, 378
MaeIII GTNAC 2 cut(s) 204, 410
MalI GATC 1 cut(s) 549
MboI GATC 1 cut(s) 547
MboII GAAGA 2 cut(s) 443, 459
MluCI AATT 2 cut(s) 342, 443
MnlI CCTC 4 cut(s) 67, 245, 283, 294
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 1 cut(s) 191
MspI CCGG 2 cut(s) 331, 339
MspR9I CCNGG 2 cut(s) 332, 339
NciI CCSGG 2 cut(s) 332, 339
NdeII GATC 1 cut(s) 547
NlaIII CATG 2 cut(s) 104, 283
NlaIV GGNNCC 2 cut(s) 336, 477
NmuCI GTSAC 1 cut(s) 204
OliI CACNNNNGTG 1 cut(s) 191
PfeI GAWTC 1 cut(s) 79
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 2 cut(s) 255, 309
PspEI GGTNACC 1 cut(s) 204
PspN4I GGNNCC 2 cut(s) 336, 477
PstI CTGCAG 1 cut(s) 312
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
RseI CAYNNNNRTG 1 cut(s) 191
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 2 cut(s) 254, 308
Sau3AI GATC 1 cut(s) 547
ScrFI CCNGG 2 cut(s) 332, 339
SetI ASST 5 cut(s) 78, 211, 226, 258, 419
SfaNI GCATC 2 cut(s) 191, 294
SfcI CTRYAG 1 cut(s) 308
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SpeI ACTAGT 2 cut(s) 139, 188
Sse9I AATT 2 cut(s) 342, 443
SsiI CCGC 1 cut(s) 202
SspMI CTAG 3 cut(s) 140, 189, 378
StyD4I CCNGG 2 cut(s) 330, 337
StyI CCWWGG 2 cut(s) 513, 560
TaaI ACNGT 1 cut(s) 53
TasI AATT 2 cut(s) 342, 443
TatI WGTACW 1 cut(s) 130
TfiI GAWTC 1 cut(s) 79
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TseFI GTSAC 1 cut(s) 204
TseI GCWGC 2 cut(s) 253, 307
Tsp45I GTSAC 1 cut(s) 204
TspDTI ATGAA 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 19
XspI CTAG 3 cut(s) 140, 189, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.