Rh6CG185900

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000458
Physical Location & Seq
Reverse (-)
30117989 .. 30125443
7455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG185900.1

Sequence Viewer

Length: 1212 bp
ATGGGTGGGATTTTCAACAAGGTCAAACGGACGCCCAAAGTAGAAGATGTCACTGTAGCTATAAATCGTCATCCTGCTAGTAATGGGTGTGATGAAATGCCCCCAAGAGCAAATGGTATTACAACTCCATCCAATTGCTTCTCTAAGCAGGCAGCTGTTGCTAAGGAACTATGTCATGAGTTGCATTCGTTGGATAGATTGGAACGAGAATATCGGCATAAGCTTGAAGAAGATAGTTCAGTTACTGTTAAAAGAGGAGACAACCTTGCAATTTTAAACGCAGATATAAAGAATCAAATGAAGCTTGTAAACAGCTTAAAGAAAAAATCACTGTGGTCAAAGACTTTTGAAGAGGTTATGCCGAAGCTTGTAGACATTGTGCAATTATTACACTTCAAAATTCATGAAGCTTTTGGAAGTACTGATGATGACGAACCAGTGGAACGTTCTGAGAGGAACTGTAAAAGGTTGGGGCCTGCTGGTACTTCATTACACTATGCGCATATTATTACACAAATTAACACACTTGTGTCTCGATCAAGGTCAGTGCCTCAAAGTGTGATAGATACTCTGTATGGGGGACTGCCATGCAGTGTAAAGTCAACTCTGCGCTCAAAATTTCAGTTAGTTGAGCTCACCATTCCTCAAATCAAAGCTGAAATGAAGCAAACTTTGGATTGGCTTGTTCCCATCGCCATTAACACAACCAAAGCTCTTCATGGCTTTGGATGGGTTGGAGAGTGGGCAAATACAGGGTTTGAGGCAAGCTGGCATACTGGCTTGACTAGCATTCCGAGGATTGGGACTCTACACCATGCCGATGAGAAAAAAACTGAAGCTTATATATGTGAACTAGTAGTCTTGCTACATTATCTGATCAGCCAGGCGGGGGTTGGCCATGGTGGAATGCCATCGTCACCTGTTGAAACCCGCAGCCAGAGGACGAATGAGTTAACAGCTGAAGATCAAGAGATGCTTAAGGATGTGGGTATAAACAGCATCAGACTAGGAATGAGTAAAAGTCATAATTTTGACGCAGGAAAGGCCAGCAGGTTGAGCAGACACCATAGGCTGAGTAAGAGTAGCAGAGACTTCCTAGCAAAAGATAAACTTCCCATTGAGAATCTGTTGTCTAGGCCCATAGACTCCGATATAACCTGGACCAAGTTCTTGGATGTCATTGATGGAGTTGACACAATGTGCAGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

403

Amino Acids

45.11

Weight (kDa)

8.56

Isoelectric Point (pI)

42.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF668 PF05003 156 - 237 1.5e-21 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34320 AT1G34320
fragaria_vesca FvH4_2g12340 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_5g36070 FvH4_5g36100 FvH4_5g36130 FvH4_5g36140
malus_domestica MD05G1039900.v1.1 MD10G1045400.v1.1 MD10G1045600.v1.1 MD10G1045700.v1.1 MD10G1045800.v1.1 MD12G1057900.v1.1
prunus_persica Prupe.1G525500_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1
pyrus_communis pycom05g03250 pycom10g03180 pycom10g03190 pycom15g33960
rosa_chinensis RchiOBHm_Chr3g0484671 RchiOBHm_Chr6g0272351 RchiOBHm_Chr6g0272361 RchiOBHm_Chr7g0237881
rosa_laevigata RLG00000000952 RLG00000000960 RLG00000001025 RLG00000008689 RLG00000010609 RLG00000013679 RLG00000013680
rosa_multiflora Rmu_sc0001339.1_g000015 Rmu_sc0002877.1_g000020 Rmu_sc0009379.1_g000003 Rmu_ssc0000199.1_g000001 Rmu_ssc0000321.1_g000001
rosa_roxburghii Rroxscaffold_1G00039160 Rroxscaffold_3G00223500 Rroxscaffold_3G00223670 Rroxscaffold_7G00196370 Rroxscaffold_7G00196380
rosa_rugosa Rorug06G0068200 Rorug06G0068300 Rorug06G0068400 Rorug07G0302100 Rorug07G0308600 Rorug07G0309200.1
rosa_samantha Rh3AG263300 Rh3DG292000 Rh6AG113700 Rh6AG184400 Rh6AG184500 Rh6BG187800 Rh6BG187900 Rh6CG185900 Rh6DG177600 Rh6DG177700 Rh7AG458500 Rh7AG464200 Rh7AG464500 Rh7BG429200 Rh7BG434200 Rh7CG476400 Rh7CG481600 Rh7CG482700 Rh7DG445700 Rh7DG450300 Rh7DG451400
rosa_wichuraiana Rw0G019450 Rw6G015900 Rw6G015910 Rw6G015940 Rw6G015950 Rw7G037980 Rw7G038410 Rw7G038470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 501
Acc36I ACCTGC 2 cut(s) 1041, 1194
AccI GTMKAC 1 cut(s) 372
AciI CCGC 2 cut(s) 887, 931
AclI AACGTT 1 cut(s) 445
AcoI YGGCCR 1 cut(s) 895
AcsI RAATTY 2 cut(s) 399, 617
AcuI CTGAAG 2 cut(s) 855, 981
AcyI GRCGYC 1 cut(s) 32
AdeI CACNNNGTG 1 cut(s) 1200
AfaI GTAC 2 cut(s) 421, 484
AfiI CCNNNNNNNGG 2 cut(s) 800, 889
AflII CTTAAG 1 cut(s) 977
AgsI TTSAA 5 cut(s) 16, 227, 350, 397, 926
AhlI ACTAGT 1 cut(s) 853
AjnI CCWGG 2 cut(s) 882, 1157
AjuI GAANNNNNNNTTGG 2 cut(s) 656, 688
AleI CACNNNNGTG 1 cut(s) 527
Alw21I GWGCWC 1 cut(s) 636
Alw26I GTCTC 3 cut(s) 252, 537, 1083
AlwNI CAGNNNCTG 1 cut(s) 245
AoxI GGCC 4 cut(s) 473, 895, 1044, 1136
ApeKI GCWGC 2 cut(s) 152, 933
ApoI RAATTY 2 cut(s) 399, 617
AspLEI GCGC 2 cut(s) 502, 612
AspS9I GGNCC 3 cut(s) 473, 1137, 1161
AsuHPI GGTGA 2 cut(s) 628, 909
AvaII GGWCC 1 cut(s) 1161
BalI TGGCCA 1 cut(s) 897
BanII GRGCYC 1 cut(s) 636
Bbv12I GWGCWC 1 cut(s) 636
BbvI GCAGC 2 cut(s) 164, 945
BccI CCATC 5 cut(s) 136, 698, 723, 919, 1178
BciT130I CCWGG 2 cut(s) 884, 1159
BclI TGATCA 1 cut(s) 876
BcoDI GTCTC 3 cut(s) 252, 537, 1083
BcuI ACTAGT 1 cut(s) 853
BfaI CTAG 6 cut(s) 78, 786, 854, 1007, 1097, 1134
BfmI CTRYAG 1 cut(s) 54
BfrI CTTAAG 1 cut(s) 977
BfuAI ACCTGC 2 cut(s) 1041, 1194
BisI GCNGC 2 cut(s) 153, 934
BlsI GCNGC 2 cut(s) 154, 935
BmcAI AGTACT 1 cut(s) 421
Bme1390I CCNGG 2 cut(s) 884, 1159
Bme18I GGWCC 1 cut(s) 1161
BmgT120I GGNCC 3 cut(s) 473, 1137, 1161
BmiI GGNNCC 1 cut(s) 474
BmrFI CCNGG 2 cut(s) 884, 1159
BmsI GCATC 2 cut(s) 963, 1008
Bpu10I CCTNAGC 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 794, 898
Bsc4I CCNNNNNNNGG 2 cut(s) 800, 889
Bse1I ACTGG 2 cut(s) 437, 781
BseBI CCWGG 2 cut(s) 884, 1159
BseDI CCNNGG 2 cut(s) 794, 898
BseGI GGATG 5 cut(s) 70, 128, 734, 988, 1180
BseLI CCNNNNNNNGG 2 cut(s) 800, 889
BseMII CTCAG 2 cut(s) 441, 1064
BseNI ACTGG 2 cut(s) 437, 781
BseRI GAGGAG 1 cut(s) 270
BseXI GCAGC 2 cut(s) 164, 945
BshFI GGCC 4 cut(s) 475, 897, 1046, 1138
BsiHKAI GWGCWC 1 cut(s) 636
BslFI GGGAC 2 cut(s) 594, 817
BslI CCNNNNNNNGG 2 cut(s) 800, 889
BsmAI GTCTC 3 cut(s) 252, 537, 1083
BsmFI GGGAC 2 cut(s) 594, 817
BsmI GAATGC 3 cut(s) 184, 789, 912
BsnI GGCC 4 cut(s) 475, 897, 1046, 1138
Bsp1286I GDGCHC 1 cut(s) 636
Bsp143I GATC 3 cut(s) 536, 876, 964
Bsp19I CCATGG 1 cut(s) 898
BspACI CCGC 2 cut(s) 887, 931
BspANI GGCC 4 cut(s) 475, 897, 1046, 1138
BspCNI CTCAG 2 cut(s) 442, 1065
BspHI TCATGA 2 cut(s) 175, 403
BspLI GGNNCC 1 cut(s) 474
BspMI ACCTGC 2 cut(s) 1041, 1194
BspQI GCTCTTC 1 cut(s) 720
BspTI CTTAAG 1 cut(s) 977
BsrI ACTGG 2 cut(s) 437, 781
BssECI CCNNGG 2 cut(s) 794, 898
BssMI GATC 3 cut(s) 536, 876, 964
BssNI GRCGYC 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 898
Bst2UI CCWGG 2 cut(s) 884, 1159
Bst4CI ACNGT 4 cut(s) 55, 247, 333, 461
Bst6I CTCTTC 2 cut(s) 345, 720
BstACI GRCGYC 1 cut(s) 32
BstAFI CTTAAG 1 cut(s) 977
BstAPI GCANNNNNTGC 1 cut(s) 158
BstC8I GCNNGC 5 cut(s) 150, 477, 766, 770, 1048
BstDEI CTNAG 4 cut(s) 144, 162, 450, 1073
BstDSI CCRYGG 1 cut(s) 898
BstF5I GGATG 5 cut(s) 70, 128, 734, 988, 1180
BstHHI GCGC 2 cut(s) 502, 612
BstKTI GATC 3 cut(s) 539, 879, 967
BstMAI GTCTC 3 cut(s) 252, 537, 1083
BstMBI GATC 3 cut(s) 536, 876, 964
BstMWI GCNNNNNNNGC 4 cut(s) 158, 786, 1043, 1056
BstNI CCWGG 2 cut(s) 884, 1159
BstSCI CCNGG 2 cut(s) 882, 1157
BstSFI CTRYAG 1 cut(s) 54
BstV1I GCAGC 2 cut(s) 164, 945
BstXI CCANNNNNNTGG 1 cut(s) 1171
BsuRI GGCC 4 cut(s) 475, 897, 1046, 1138
BtgI CCRYGG 1 cut(s) 898
BtgZI GCGATG 1 cut(s) 676
BtsCI GGATG 5 cut(s) 70, 128, 734, 988, 1180
BtsI GCAGTG 1 cut(s) 598
BtsIMutI CAGTG 5 cut(s) 51, 329, 444, 552, 598
BveI ACCTGC 2 cut(s) 1041, 1194
Cac8I GCNNGC 5 cut(s) 150, 477, 766, 770, 1048
CaiI CAGNNNCTG 1 cut(s) 245
CciI TCATGA 2 cut(s) 175, 403
CfoI GCGC 2 cut(s) 502, 612
Cfr13I GGNCC 3 cut(s) 473, 1137, 1161
CseI GACGC 2 cut(s) 40, 1043
Csp6I GTAC 2 cut(s) 420, 483
CviAII CATG 6 cut(s) 176, 404, 588, 719, 815, 899
CviQI GTAC 2 cut(s) 420, 483
DdeI CTNAG 4 cut(s) 144, 162, 450, 1073
DpnI GATC 3 cut(s) 538, 878, 966
DpnII GATC 3 cut(s) 536, 876, 964
DraI TTTAAA 1 cut(s) 276
DraIII CACNNNGTG 1 cut(s) 1200
EaeI YGGCCR 1 cut(s) 895
Eam1104I CTCTTC 2 cut(s) 345, 720
EarI CTCTTC 2 cut(s) 345, 720
Ecl136II GAGCTC 1 cut(s) 634
Eco130I CCWWGG 1 cut(s) 898
Eco24I GRGCYC 1 cut(s) 636
Eco47I GGWCC 1 cut(s) 1161
Eco53kI GAGCTC 1 cut(s) 634
Eco57I CTGAAG 2 cut(s) 855, 981
EcoICRI GAGCTC 1 cut(s) 634
EcoO109I RGGNCCY 1 cut(s) 473
EcoRII CCWGG 2 cut(s) 882, 1157
EcoT14I CCWWGG 1 cut(s) 898
EcoT38I GRGCYC 1 cut(s) 636
ErhI CCWWGG 1 cut(s) 898
FaeI CATG 6 cut(s) 179, 407, 591, 722, 818, 902
FalI AAGNNNNNCTT 4 cut(s) 960, 992, 1095, 1127
FaqI GGGAC 2 cut(s) 594, 817
FatI CATG 6 cut(s) 175, 403, 587, 718, 814, 898
FauI CCCGC 2 cut(s) 880, 938
FbaI TGATCA 1 cut(s) 876
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 2 cut(s) 153, 934
FokI GGATG 5 cut(s) 57, 115, 741, 995, 1187
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 2 cut(s) 153, 934
FspAI RTGCGCAY 1 cut(s) 501
FspBI CTAG 6 cut(s) 78, 786, 854, 1007, 1097, 1134
FspI TGCGCA 1 cut(s) 501
GlaI GCGC 2 cut(s) 501, 611
GluI GCNGC 2 cut(s) 153, 934
HaeIII GGCC 4 cut(s) 475, 897, 1046, 1138
HgaI GACGC 2 cut(s) 40, 1043
HhaI GCGC 2 cut(s) 502, 612
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 6 cut(s) 179, 407, 591, 722, 818, 902
Hin6I GCGC 2 cut(s) 500, 610
HinP1I GCGC 2 cut(s) 500, 610
HincII GTYRAC 3 cut(s) 603, 954, 1192
HindII GTYRAC 3 cut(s) 603, 954, 1192
HindIII AAGCTT 5 cut(s) 221, 302, 365, 408, 837
HinfI GANTC 4 cut(s) 292, 805, 1123, 1145
HpaI GTTAAC 1 cut(s) 954
HphI GGTGA 2 cut(s) 628, 909
Hpy166II GTNNAC 6 cut(s) 310, 373, 603, 851, 954, 1192
Hpy188I TCNGA 5 cut(s) 451, 795, 876, 1004, 1150
Hpy188III TCNNGA 4 cut(s) 176, 404, 534, 968
Hpy8I GTNNAC 6 cut(s) 310, 373, 603, 851, 954, 1192
HpyCH4III ACNGT 4 cut(s) 55, 247, 333, 461
HpyCH4IV ACGT 1 cut(s) 445
HpyCH4V TGCA 5 cut(s) 184, 269, 382, 591, 1203
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 786, 1043, 1056
HpyF3I CTNAG 4 cut(s) 144, 162, 450, 1073
HpySE526I ACGT 1 cut(s) 445
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 6 cut(s) 179, 407, 591, 722, 818, 902
HspAI GCGC 2 cut(s) 500, 610
Ksp22I TGATCA 1 cut(s) 876
KspAI GTTAAC 1 cut(s) 954
Kzo9I GATC 3 cut(s) 536, 876, 964
LguI GCTCTTC 1 cut(s) 720
Lsp1109I GCAGC 2 cut(s) 164, 945
LweI GCATC 2 cut(s) 963, 1008
MaeI CTAG 6 cut(s) 78, 786, 854, 1007, 1097, 1134
MaeII ACGT 1 cut(s) 445
MaeIII GTNAC 3 cut(s) 49, 241, 915
MalI GATC 3 cut(s) 538, 878, 966
MboI GATC 3 cut(s) 536, 876, 964
MboII GAAGA 6 cut(s) 56, 239, 242, 362, 707, 974
MfeI CAATTG 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 636
MlsI TGGCCA 1 cut(s) 897
MluCI AATT 7 cut(s) 133, 270, 383, 399, 516, 617, 1027
MluNI TGGCCA 1 cut(s) 897
MlyI GAGTC 2 cut(s) 799, 1139
MmeI TCCRAC 2 cut(s) 171, 715
MnlI CCTC 8 cut(s) 248, 346, 447, 561, 654, 754, 789, 933
Mox20I TGGCCA 1 cut(s) 897
MscI TGGCCA 1 cut(s) 897
MseI TTAA 7 cut(s) 249, 275, 317, 519, 699, 953, 978
MslI CAYNNNNRTG 2 cut(s) 527, 819
Msp20I TGGCCA 1 cut(s) 897
MspA1I CMGCKG 2 cut(s) 155, 959
MspCI CTTAAG 1 cut(s) 977
MspR9I CCNGG 2 cut(s) 884, 1159
MunI CAATTG 1 cut(s) 133
Mva1269I GAATGC 3 cut(s) 184, 789, 912
MvaI CCWGG 2 cut(s) 884, 1159
MwoI GCNNNNNNNGC 4 cut(s) 158, 786, 1043, 1056
NcoI CCATGG 1 cut(s) 898
NdeII GATC 3 cut(s) 536, 876, 964
NlaIII CATG 6 cut(s) 179, 407, 591, 722, 818, 902
NlaIV GGNNCC 1 cut(s) 474
NmuCI GTSAC 2 cut(s) 49, 915
NsbI TGCGCA 1 cut(s) 501
OliI CACNNNNGTG 1 cut(s) 527
PagI TCATGA 2 cut(s) 175, 403
PciSI GCTCTTC 1 cut(s) 720
PctI GAATGC 3 cut(s) 184, 789, 912
PfeI GAWTC 2 cut(s) 292, 1123
PkrI GCNGC 2 cut(s) 154, 935
PleI GAGTC 2 cut(s) 799, 1139
PpsI GAGTC 2 cut(s) 799, 1139
Psp124BI GAGCTC 1 cut(s) 636
Psp1406I AACGTT 1 cut(s) 445
Psp6I CCWGG 2 cut(s) 882, 1157
PspGI CCWGG 2 cut(s) 882, 1157
PspN4I GGNNCC 1 cut(s) 474
PspPI GGNCC 3 cut(s) 473, 1137, 1161
PstNI CAGNNNCTG 1 cut(s) 245
PvuII CAGCTG 2 cut(s) 155, 959
RsaI GTAC 2 cut(s) 421, 484
RsaNI GTAC 2 cut(s) 420, 483
RseI CAYNNNNRTG 2 cut(s) 527, 819
SacI GAGCTC 1 cut(s) 636
SapI GCTCTTC 1 cut(s) 720
SaqAI TTAA 7 cut(s) 249, 275, 317, 519, 699, 953, 978
SatI GCNGC 2 cut(s) 153, 934
Sau3AI GATC 3 cut(s) 536, 876, 964
Sau96I GGNCC 3 cut(s) 473, 1137, 1161
ScaI AGTACT 1 cut(s) 421
SchI GAGTC 2 cut(s) 799, 1139
ScrFI CCNGG 2 cut(s) 884, 1159
SduI GDGCHC 1 cut(s) 636
SfaNI GCATC 2 cut(s) 963, 1008
SfcI CTRYAG 1 cut(s) 54
SinI GGWCC 1 cut(s) 1161
SmiMI CAYNNNNRTG 2 cut(s) 527, 819
SmlI CTYRAG 1 cut(s) 977
SmoI CTYRAG 1 cut(s) 977
SpeI ACTAGT 1 cut(s) 853
Sse9I AATT 7 cut(s) 133, 270, 383, 399, 516, 617, 1027
SsiI CCGC 2 cut(s) 887, 931
SspMI CTAG 6 cut(s) 78, 786, 854, 1007, 1097, 1134
SstI GAGCTC 1 cut(s) 636
StyD4I CCNGG 2 cut(s) 882, 1157
StyI CCWWGG 1 cut(s) 898
TaaI ACNGT 4 cut(s) 55, 247, 333, 461
TaiI ACGT 1 cut(s) 448
TaqI TCGA 1 cut(s) 535
TasI AATT 7 cut(s) 133, 270, 383, 399, 516, 617, 1027
TatI WGTACW 1 cut(s) 419
TfiI GAWTC 2 cut(s) 292, 1123
Tru1I TTAA 7 cut(s) 249, 275, 317, 519, 699, 953, 978
Tru9I TTAA 7 cut(s) 249, 275, 317, 519, 699, 953, 978
TscAI CASTG 5 cut(s) 58, 336, 444, 552, 598
TseFI GTSAC 2 cut(s) 49, 915
TseI GCWGC 2 cut(s) 152, 933
Tsp45I GTSAC 2 cut(s) 49, 915
TspDTI ATGAA 7 cut(s) 108, 314, 392, 420, 477, 677, 707
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 5 cut(s) 58, 336, 444, 552, 598
Vha464I CTTAAG 1 cut(s) 977
VpaK11BI GGWCC 1 cut(s) 1161
XapI RAATTY 2 cut(s) 399, 617
XcmI CCANNNNNNNNNTGG 1 cut(s) 890
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 6 cut(s) 78, 786, 854, 1007, 1097, 1134
ZrmI AGTACT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.