MD10G1045600.v1.1

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000458
Physical Location & Seq
Forward (+)
6108737 .. 6123464
14728 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1045600.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGATATCGGAAATGAGCTCACTGCTGGGCAGGGCTAGTACATCTGGCCTTGGGAGGGCAGTGGATGCAATCTATGGCCTTGGTAGTAGCATGGCAATTCTGAACACAAACACTGGTTTTACCTCTGCGGCGACAACAAAAGGGACTAAAATTTCACTCTTGACTTTCGAAGTTGCAAACACAACTGTCAAGGGATCAAATTTAATGCAATCATTGTCAACGTATAACATTAAACATTTAAAAGAGGTTGTGCTTCCATCAGAAGGGGTCCAGAATTTAGTTTCACGAAATATGGATGAACTCCTGAGAATTGTTGCAGCTGACAAGAGGGAAGAGCTCAAAATTCTCTCCGGAGAAGTAGCGCGGTTTGGAAATTGTTGTAAAGACCCTCAGTGGCATAATCTGGATCGCTATCTTAACCAGTGCCCTTTCACAAACATTATGGTTGCACGACAGAAGCTATTGAAGGAAGATGCAGAGGTGGTAATGCAGCAAATGATGATGTTGGTTTCAAATACACATGAATTATATCATGAGATTTGTTCCTTGGACAAACTTGACAAAGAGTACCGGTGTAAGCTTCAAGAGCAAATAGAGAATCCAGAAACCTGCCAAAGAGGAACCGATGATGAACCTGTGAATGATTCTCCGAATAATCACGAAACATTGGGGTCTGCTGGTCTTGCCTTGCATTATGCAAATATTATCACTCAAATTGATACGCTTGTGTCTCGATCAAGTTCTGTGTGTTCAAACACACGCGATACTTTATATCAGGGGCTCCCGCCTGGTGTGAAATCAGCTTTGCGATCAAAACTGCAGTCATTTCAGTTTAAGGAAGAGCATACAATCGCTGAAATTAAAGCGGAAATGAAGAAAACGTTGCAGTGGCTAGTTCCCATTGCCACTAACACAACCAAAGCTCATTATTGCTCTGGCTGGGTTGGAGAATGGGCGATGAACTGGAAACCTGCTGGTACTGACTTGCTGAGGATTGAGACACTGCACCATGCTGATAAAAATAGAACTGAGGCTTACATTCTTGAACTGGTGGTGTGGCTCCACCATCTCGTCAACCAATCGAGGGTTAGTATTGGAAGTATTCAAAGACCTCTAGGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

375

Amino Acids

41.8

Weight (kDa)

6.64

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 54 - 109 5.9e-21 Domain of unknown function (DUF3475)
DUF668 PF05003 222 - 307 4.4e-33 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34320 AT1G34320
fragaria_vesca FvH4_2g12340 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_5g36070 FvH4_5g36100 FvH4_5g36130 FvH4_5g36140
malus_domestica MD05G1039900.v1.1 MD10G1045400.v1.1 MD10G1045600.v1.1 MD10G1045700.v1.1 MD10G1045800.v1.1 MD12G1057900.v1.1
prunus_persica Prupe.1G525500_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1
pyrus_communis pycom05g03250 pycom10g03180 pycom10g03190 pycom15g33960
rosa_chinensis RchiOBHm_Chr3g0484671 RchiOBHm_Chr6g0272351 RchiOBHm_Chr6g0272361 RchiOBHm_Chr7g0237881
rosa_laevigata RLG00000000952 RLG00000000960 RLG00000001025 RLG00000008689 RLG00000010609 RLG00000013679 RLG00000013680
rosa_multiflora Rmu_sc0001339.1_g000015 Rmu_sc0002877.1_g000020 Rmu_sc0009379.1_g000003 Rmu_ssc0000199.1_g000001 Rmu_ssc0000321.1_g000001
rosa_roxburghii Rroxscaffold_1G00039160 Rroxscaffold_3G00223500 Rroxscaffold_3G00223670 Rroxscaffold_7G00196370 Rroxscaffold_7G00196380
rosa_rugosa Rorug06G0068200 Rorug06G0068300 Rorug06G0068400 Rorug07G0302100 Rorug07G0308600 Rorug07G0309200.1
rosa_samantha Rh3AG263300 Rh3DG292000 Rh6AG113700 Rh6AG184400 Rh6AG184500 Rh6BG187800 Rh6BG187900 Rh6CG185900 Rh6DG177600 Rh6DG177700 Rh7AG458500 Rh7AG464200 Rh7AG464500 Rh7BG429200 Rh7BG434200 Rh7CG476400 Rh7CG481600 Rh7CG482700 Rh7DG445700 Rh7DG450300 Rh7DG451400
rosa_wichuraiana Rw0G019450 Rw6G015900 Rw6G015910 Rw6G015940 Rw6G015950 Rw7G037980 Rw7G038410 Rw7G038470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 617, 979
AccII CGCG 2 cut(s) 364, 762
AccIII TCCGGA 1 cut(s) 350
AciI CCGC 4 cut(s) 128, 364, 785, 866
AclI AACGTT 1 cut(s) 881
AclWI GGATC 2 cut(s) 202, 414
AcsI RAATTY 4 cut(s) 150, 199, 274, 342
AfaI GTAC 3 cut(s) 40, 569, 979
AfiI CCNNNNNNNGG 3 cut(s) 55, 263, 1084
AgeI ACCGGT 1 cut(s) 570
AgsI TTSAA 6 cut(s) 466, 513, 584, 753, 1046, 1106
AjnI CCWGG 1 cut(s) 787
AluBI AGCT 7 cut(s) 18, 320, 337, 460, 580, 803, 923
AluI AGCT 7 cut(s) 18, 320, 337, 460, 580, 803, 923
Alw21I GWGCWC 2 cut(s) 20, 339
Alw26I GTCTC 2 cut(s) 735, 992
AlwI GGATC 2 cut(s) 202, 414
Aor13HI TCCGGA 1 cut(s) 350
AoxI GGCC 2 cut(s) 46, 76
ApeKI GCWGC 2 cut(s) 317, 490
ApoI RAATTY 4 cut(s) 150, 199, 274, 342
ArsI GACNNNNNNTTYG 2 cut(s) 806, 838
AsiGI ACCGGT 1 cut(s) 570
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 1 cut(s) 268
AsuII TTCGAA 1 cut(s) 168
AvaII GGWCC 1 cut(s) 268
BaeGI GKGCMC 1 cut(s) 428
BaeI ACNNNNGTAYC 2 cut(s) 711, 744
BanII GRGCYC 3 cut(s) 20, 339, 783
Bbv12I GWGCWC 2 cut(s) 20, 339
BbvCI CCTCAGC 1 cut(s) 989
BbvI GCAGC 2 cut(s) 329, 502
BccI CCATC 2 cut(s) 265, 1074
BciT130I CCWGG 1 cut(s) 789
BcoDI GTCTC 2 cut(s) 735, 992
BfaI CTAG 3 cut(s) 36, 893, 1115
BfmI CTRYAG 1 cut(s) 818
BfuAI ACCTGC 2 cut(s) 617, 979
BisI GCNGC 3 cut(s) 129, 318, 491
BlsI GCNGC 3 cut(s) 130, 319, 492
Bme1390I CCNGG 1 cut(s) 789
Bme18I GGWCC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 4 cut(s) 269, 622, 782, 1061
BmrFI CCNGG 1 cut(s) 789
BmsI GCATC 2 cut(s) 55, 463
Bpu10I CCTNAGC 1 cut(s) 989
Bpu14I TTCGAA 1 cut(s) 168
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 3 cut(s) 49, 79, 546
BsaWI WCCGGW 2 cut(s) 350, 570
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 263, 1084
Bse118I RCCGGY 1 cut(s) 570
Bse1I ACTGG 4 cut(s) 118, 421, 968, 1053
Bse3DI GCAATG 1 cut(s) 900
Bse8I GATNNNNATC 1 cut(s) 411
BseAI TCCGGA 1 cut(s) 350
BseBI CCWGG 1 cut(s) 789
BseDI CCNNGG 3 cut(s) 49, 79, 546
BseGI GGATG 2 cut(s) 70, 301
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 3 cut(s) 55, 263, 1084
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 4 cut(s) 296, 404, 980, 1020
BseNI ACTGG 4 cut(s) 118, 421, 968, 1053
BseSI GKGCMC 1 cut(s) 428
BseXI GCAGC 2 cut(s) 329, 502
BseYI CCCAGC 2 cut(s) 25, 939
BsgI GTGCAG 1 cut(s) 989
Bsh1236I CGCG 2 cut(s) 364, 762
BshFI GGCC 2 cut(s) 48, 78
BshTI ACCGGT 1 cut(s) 570
BsiHKAI GWGCWC 2 cut(s) 20, 339
BsiSI CCGG 2 cut(s) 351, 571
BslFI GGGAC 1 cut(s) 157
BslI CCNNNNNNNGG 3 cut(s) 55, 263, 1084
BsmAI GTCTC 2 cut(s) 735, 992
BsmFI GGGAC 1 cut(s) 157
BsnI GGCC 2 cut(s) 48, 78
Bsp119I TTCGAA 1 cut(s) 168
Bsp1286I GDGCHC 4 cut(s) 20, 339, 428, 783
Bsp13I TCCGGA 1 cut(s) 350
Bsp143I GATC 4 cut(s) 194, 406, 734, 809
BspACI CCGC 4 cut(s) 128, 364, 785, 866
BspANI GGCC 2 cut(s) 48, 78
BspCNI CTCAG 4 cut(s) 297, 403, 981, 1021
BspEI TCCGGA 1 cut(s) 350
BspFNI CGCG 2 cut(s) 364, 762
BspHI TCATGA 1 cut(s) 532
BspLI GGNNCC 4 cut(s) 269, 622, 782, 1061
BspMAI CTGCAG 1 cut(s) 822
BspMI ACCTGC 2 cut(s) 617, 979
BspPI GGATC 2 cut(s) 202, 414
BspQI GCTCTTC 2 cut(s) 327, 834
BspT104I TTCGAA 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 900
BsrFI RCCGGY 1 cut(s) 570
BsrI ACTGG 4 cut(s) 118, 421, 968, 1053
BssAI RCCGGY 1 cut(s) 570
BssECI CCNNGG 3 cut(s) 49, 79, 546
BssMI GATC 4 cut(s) 194, 406, 734, 809
BssT1I CCWWGG 3 cut(s) 49, 79, 546
Bst2UI CCWGG 1 cut(s) 789
Bst4CI ACNGT 1 cut(s) 187
Bst6I CTCTTC 2 cut(s) 327, 834
BstAPI GCANNNNNTGC 1 cut(s) 65
BstBI TTCGAA 1 cut(s) 168
BstDEI CTNAG 4 cut(s) 305, 390, 989, 1029
BstF5I GGATG 2 cut(s) 70, 301
BstFNI CGCG 2 cut(s) 364, 762
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 4 cut(s) 197, 409, 737, 812
BstMAI GTCTC 2 cut(s) 735, 992
BstMBI GATC 4 cut(s) 194, 406, 734, 809
BstMWI GCNNNNNNNGC 3 cut(s) 65, 586, 683
BstNI CCWGG 1 cut(s) 789
BstSCI CCNGG 1 cut(s) 787
BstSFI CTRYAG 1 cut(s) 818
BstSLI GKGCMC 1 cut(s) 428
BstUI CGCG 2 cut(s) 364, 762
BstV1I GCAGC 2 cut(s) 329, 502
BsuRI GGCC 2 cut(s) 48, 78
BtgZI GCGATG 1 cut(s) 971
BtsCI GGATG 2 cut(s) 70, 301
BtsI GCAGTG 4 cut(s) 20, 66, 893, 1001
BtsIMutI CAGTG 7 cut(s) 20, 66, 111, 398, 428, 893, 1001
BveI ACCTGC 2 cut(s) 617, 979
CciI TCATGA 1 cut(s) 532
CfoI GCGC 1 cut(s) 364
Cfr10I RCCGGY 1 cut(s) 570
Cfr13I GGNCC 1 cut(s) 268
Csp6I GTAC 3 cut(s) 39, 568, 978
CspAI ACCGGT 1 cut(s) 570
CviAII CATG 4 cut(s) 91, 521, 533, 1010
CviQI GTAC 3 cut(s) 39, 568, 978
DdeI CTNAG 4 cut(s) 305, 390, 989, 1029
DpnI GATC 4 cut(s) 196, 408, 736, 811
DpnII GATC 4 cut(s) 194, 406, 734, 809
DraI TTTAAA 1 cut(s) 240
Eam1104I CTCTTC 2 cut(s) 327, 834
EarI CTCTTC 2 cut(s) 327, 834
Ecl136II GAGCTC 2 cut(s) 18, 337
Eco130I CCWWGG 3 cut(s) 49, 79, 546
Eco24I GRGCYC 3 cut(s) 20, 339, 783
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 1 cut(s) 268
Eco53kI GAGCTC 2 cut(s) 18, 337
EcoICRI GAGCTC 2 cut(s) 18, 337
EcoRII CCWGG 1 cut(s) 787
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 3 cut(s) 49, 79, 546
EcoT38I GRGCYC 3 cut(s) 20, 339, 783
ErhI CCWWGG 3 cut(s) 49, 79, 546
FaeI CATG 4 cut(s) 94, 524, 536, 1013
FaqI GGGAC 1 cut(s) 157
FatI CATG 4 cut(s) 90, 520, 532, 1009
FauI CCCGC 1 cut(s) 792
Fnu4HI GCNGC 3 cut(s) 129, 318, 491
FokI GGATG 2 cut(s) 77, 308
FriOI GRGCYC 3 cut(s) 20, 339, 783
Fsp4HI GCNGC 3 cut(s) 129, 318, 491
FspBI CTAG 3 cut(s) 36, 893, 1115
GlaI GCGC 1 cut(s) 363
GluI GCNGC 3 cut(s) 129, 318, 491
GsaI CCCAGC 2 cut(s) 29, 943
HaeIII GGCC 2 cut(s) 48, 78
HapII CCGG 2 cut(s) 351, 571
HhaI GCGC 1 cut(s) 364
Hin1II CATG 4 cut(s) 94, 524, 536, 1013
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HincII GTYRAC 2 cut(s) 219, 1075
HindII GTYRAC 2 cut(s) 219, 1075
HindIII AAGCTT 1 cut(s) 578
HinfI GANTC 2 cut(s) 598, 644
HpaII CCGG 2 cut(s) 351, 571
Hpy166II GTNNAC 2 cut(s) 219, 1075
Hpy188I TCNGA 4 cut(s) 10, 102, 262, 651
Hpy8I GTNNAC 2 cut(s) 219, 1075
HpyAV CCTTC 2 cut(s) 257, 460
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4IV ACGT 2 cut(s) 221, 881
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 586, 683
HpyF3I CTNAG 4 cut(s) 305, 390, 989, 1029
HpySE526I ACGT 2 cut(s) 221, 881
Hsp92II CATG 4 cut(s) 94, 524, 536, 1013
HspAI GCGC 1 cut(s) 362
Kpn2I TCCGGA 1 cut(s) 350
Kzo9I GATC 4 cut(s) 194, 406, 734, 809
LguI GCTCTTC 2 cut(s) 327, 834
LmnI GCTCC 2 cut(s) 786, 1065
Lsp1109I GCAGC 2 cut(s) 329, 502
LweI GCATC 2 cut(s) 55, 463
MaeI CTAG 3 cut(s) 36, 893, 1115
MaeII ACGT 2 cut(s) 221, 881
MalI GATC 4 cut(s) 196, 408, 736, 811
MboI GATC 4 cut(s) 194, 406, 734, 809
MboII GAAGA 4 cut(s) 344, 482, 851, 886
MhlI GDGCHC 4 cut(s) 20, 339, 428, 783
MmeI TCCRAC 1 cut(s) 925
MroI TCCGGA 1 cut(s) 350
MseI TTAA 6 cut(s) 203, 231, 239, 417, 834, 861
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 2 cut(s) 351, 571
MspR9I CCNGG 1 cut(s) 789
MvaI CCWGG 1 cut(s) 789
MvnI CGCG 2 cut(s) 364, 762
MwoI GCNNNNNNNGC 3 cut(s) 65, 586, 683
NdeII GATC 4 cut(s) 194, 406, 734, 809
NlaIII CATG 4 cut(s) 94, 524, 536, 1013
NlaIV GGNNCC 4 cut(s) 269, 622, 782, 1061
NspV TTCGAA 1 cut(s) 168
PagI TCATGA 1 cut(s) 532
PciSI GCTCTTC 2 cut(s) 327, 834
PfeI GAWTC 2 cut(s) 598, 644
PinAI ACCGGT 1 cut(s) 570
PkrI GCNGC 3 cut(s) 130, 319, 492
Psp124BI GAGCTC 2 cut(s) 20, 339
Psp1406I AACGTT 1 cut(s) 881
Psp6I CCWGG 1 cut(s) 787
PspFI CCCAGC 2 cut(s) 25, 939
PspGI CCWGG 1 cut(s) 787
PspN4I GGNNCC 4 cut(s) 269, 622, 782, 1061
PspPI GGNCC 1 cut(s) 268
PstI CTGCAG 1 cut(s) 822
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 3 cut(s) 40, 569, 979
RsaNI GTAC 3 cut(s) 39, 568, 978
SacI GAGCTC 2 cut(s) 20, 339
SapI GCTCTTC 2 cut(s) 327, 834
SaqAI TTAA 6 cut(s) 203, 231, 239, 417, 834, 861
SatI GCNGC 3 cut(s) 129, 318, 491
Sau3AI GATC 4 cut(s) 194, 406, 734, 809
Sau96I GGNCC 1 cut(s) 268
ScrFI CCNGG 1 cut(s) 789
SduI GDGCHC 4 cut(s) 20, 339, 428, 783
SfaNI GCATC 2 cut(s) 55, 463
SfcI CTRYAG 1 cut(s) 818
SfuI TTCGAA 1 cut(s) 168
SinI GGWCC 1 cut(s) 268
SsiI CCGC 4 cut(s) 128, 364, 785, 866
SspI AATATT 1 cut(s) 703
SspMI CTAG 3 cut(s) 36, 893, 1115
SstI GAGCTC 2 cut(s) 20, 339
StyD4I CCNGG 1 cut(s) 787
StyI CCWWGG 3 cut(s) 49, 79, 546
TaaI ACNGT 1 cut(s) 187
TaiI ACGT 2 cut(s) 224, 884
TaqI TCGA 3 cut(s) 168, 733, 1082
TatI WGTACW 1 cut(s) 38
TauI GCSGC 1 cut(s) 131
TfiI GAWTC 2 cut(s) 598, 644
Tru1I TTAA 6 cut(s) 203, 231, 239, 417, 834, 861
Tru9I TTAA 6 cut(s) 203, 231, 239, 417, 834, 861
TscAI CASTG 7 cut(s) 27, 66, 118, 398, 428, 893, 1008
TseI GCWGC 2 cut(s) 317, 490
TspDTI ATGAA 5 cut(s) 312, 537, 645, 887, 974
TspRI CASTG 7 cut(s) 27, 66, 118, 398, 428, 893, 1008
VpaK11BI GGWCC 1 cut(s) 268
XapI RAATTY 4 cut(s) 150, 199, 274, 342
XspI CTAG 3 cut(s) 36, 893, 1115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.