Prupe.8G052600_v2.0.a1

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000458
Physical Location & Seq
Forward (+)
5797486 .. 5806524
9039 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G052600.2

Sequence Viewer

Length: 1773 bp
ATGGGTGGAATTCGCACCACAGTGAAACCGGCATTGAAAACGGGGCCCAAGGTAAAATATGTCATTATAGATTACCCTCGAGATAGCAATGAGTTTGATGGACTGTCCCCAAGAGCAATTAGTAGTACAACTACATGCCCGGCCATTCCTAAGCAGGCTGCAAAGGTTTCAGAAGTGAGCTCACTTTTGGGCAAAGCTGGCATCACTGGGTTCGGGAAGGCTATACAATTTTTGGACAGTCTTGGTAGCAGCATGACAAATTTGACCTCCATTGTTGGTACCTCAGGAATGTCAAGGAAAGAGAATACAATTTCAATTTTGGCTTTTGAAGTTGCAAACACAATTGTCAAGGGTGCCAACCTGATGCAATCCCTTTCAGAGGATAATGTCAGACATTTAAAGGAGGTGGTGCTACCATCAAAAGGGGTGCAAACTTTAATATCTAGAGATATGGATGAGCTCCTGCGAATTGCTGCTGCTGACAAGAGAGAAGAACTGAAAACATTTTCCAGTGACGTTGTGCGTTTTGGAAATTATTCTAAAGATCTGATGTGGCACAATTTGGACCTTTATTTTAAGAAGATTGGTTCAAAAGTTACACAACAGAAGCATTTCAAAAGGCAAGCACAGGAAGATATGGAACAATTGATGACTTTAGTCCAGTCAACAGCAGAATTATGTTATGAGTTGCATATGTTGGACAGACTGGAACAAGATTATCAGCATAGGCTTAAAGGAGATGGTTCAAATGCTGCTAGAAGAGGAGACAGCCTTGCAATTTTAAGAGCAGATGTAAAGATGCAAGAGAAGTTTGTAAGCAGTTTAAAGAAAAAATCACTCTGGTCAAAGACTTTTGAAGAGGTTGTCCAGAAGCTTGTAGATATTGTACACTTTCTACATTTGGAAATCCATGAAGCTTTTGGCAGTGCTGATGATGAACCTGTGGAGAGTTCTCGGAGGAACCATAAGAAGTTGGGGCCTTCTGGTATTTCATTACATTATGCGAGTATTATTACACAAATTAACACACTAGTGTCTCTGTCACGATCGAGTTCAGTGCCTCAACATATGAGGGATACTTTGTATCAAGGGCTGCCATCCAGTGTAAAGTCAGCTTTGCGCTCAAAATTACAGTTAGTTGAGCTCACTATCCCTCAAATCAAAACTGAAATGAAGAAAACATTGGAGTGGCTTGTTCCCATTGCCAGTAACACAACCAAAGCTCTTCATAGCTTTGGCTGGGTTGGAGAGTGGGCAAATTCAGAGTTTGGGGAACGCTGGAAACATGGTGGCCAGACTGGCATTCTGAGGATTGAGACACTACACCATGCAGATAAGGCAGAAACTGAAGCTTGTATCTTTAAACTAGTAGTTTGGCTACAGCATCTGATCAGCCAGTCGGGGGCTAGTGACAATGGAATGCCGTCAACTCCTGTTAAAACCCTAAACCAGAAGACAAATCTGTTATCTAGACACACACCCAACGGTTCATCCCCCGTTTTGACAGATGAAGATAAAGAGATGCTTCAAGATGTGAGTAAGAGCAACTTGAGGCTCAGGATGAGTAGGAGTCAGAATTATGCCTCAAGAAACGCCATGTTAAGCAAACAACATAGGCTGAGTAAGAGCAGCCGAGACTTCCTATCAAGTAAAACCAGGAAAGATCCATTTCCCGTATGGAGTCTGCTATCTAGGCCGATAGACTTTGATAACGATCGGATCAAACTCTTGGATGTCATTGATGGAGTTGACACAATCAAAGGTTCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

591

Amino Acids

65.97

Weight (kDa)

9.41

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34320 AT1G34320
fragaria_vesca FvH4_2g12340 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_5g36070 FvH4_5g36100 FvH4_5g36130 FvH4_5g36140
malus_domestica MD05G1039900.v1.1 MD10G1045400.v1.1 MD10G1045600.v1.1 MD10G1045700.v1.1 MD10G1045800.v1.1 MD12G1057900.v1.1
prunus_persica Prupe.1G525500_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1
pyrus_communis pycom05g03250 pycom10g03180 pycom10g03190 pycom15g33960
rosa_chinensis RchiOBHm_Chr3g0484671 RchiOBHm_Chr6g0272351 RchiOBHm_Chr6g0272361 RchiOBHm_Chr7g0237881
rosa_laevigata RLG00000000952 RLG00000000960 RLG00000001025 RLG00000008689 RLG00000010609 RLG00000013679 RLG00000013680
rosa_multiflora Rmu_sc0001339.1_g000015 Rmu_sc0002877.1_g000020 Rmu_sc0009379.1_g000003 Rmu_ssc0000199.1_g000001 Rmu_ssc0000321.1_g000001
rosa_roxburghii Rroxscaffold_1G00039160 Rroxscaffold_3G00223500 Rroxscaffold_3G00223670 Rroxscaffold_7G00196370 Rroxscaffold_7G00196380
rosa_rugosa Rorug06G0068200 Rorug06G0068300 Rorug06G0068400 Rorug07G0302100 Rorug07G0308600 Rorug07G0309200.1
rosa_samantha Rh3AG263300 Rh3DG292000 Rh6AG113700 Rh6AG184400 Rh6AG184500 Rh6BG187800 Rh6BG187900 Rh6CG185900 Rh6DG177600 Rh6DG177700 Rh7AG458500 Rh7AG464200 Rh7AG464500 Rh7BG429200 Rh7BG434200 Rh7CG476400 Rh7CG481600 Rh7CG482700 Rh7DG445700 Rh7DG450300 Rh7DG451400
rosa_wichuraiana Rw0G019450 Rw6G015900 Rw6G015910 Rw6G015940 Rw6G015950 Rw7G037980 Rw7G038410 Rw7G038470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 278
AccB1I GGYRCC 2 cut(s) 278, 353
AclWI GGATC 2 cut(s) 1658, 1727
AcoI YGGCCR 2 cut(s) 141, 1291
AcsI RAATTY 3 cut(s) 9, 259, 1258
AcuI CTGAAG 1 cut(s) 1368
AfaI GTAC 3 cut(s) 127, 280, 888
AfiI CCNNNNNNNGG 3 cut(s) 379, 422, 1402
AgsI TTSAA 8 cut(s) 37, 315, 329, 591, 616, 747, 857, 1529
AhlI ACTAGT 2 cut(s) 1030, 1366
AjnI CCWGG 1 cut(s) 1655
AjuI GAANNNNNNNTTGG 2 cut(s) 1166, 1198
AleI CACNNNNGTG 2 cut(s) 20, 1031
Alw21I GWGCWC 3 cut(s) 182, 462, 1146
Alw26I GTCTC 4 cut(s) 759, 1041, 1310, 1629
AlwI GGATC 2 cut(s) 1658, 1727
AlwNI CAGNNNCTG 2 cut(s) 1346, 1387
Ama87I CYCGRG 1 cut(s) 78
AoxI GGCC 5 cut(s) 44, 141, 977, 1291, 1694
ApaI GGGCCC 1 cut(s) 48
ApeKI GCWGC 7 cut(s) 158, 249, 473, 476, 752, 1093, 1629
ApoI RAATTY 3 cut(s) 9, 259, 1258
Asp700I GAANNNNTTC 2 cut(s) 535, 611
Asp718I GGTACC 1 cut(s) 278
AspLEI GCGC 1 cut(s) 1122
AspS9I GGNCC 4 cut(s) 44, 45, 565, 977
AsuC2I CCSGG 1 cut(s) 140
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 1 cut(s) 565
AxyI CCTNAGG 1 cut(s) 283
BaeGI GKGCMC 1 cut(s) 48
BalI TGGCCA 1 cut(s) 1293
BanI GGYRCC 2 cut(s) 278, 353
BanII GRGCYC 4 cut(s) 48, 182, 462, 1146
BbsI GAAGAC 1 cut(s) 1460
Bbv12I GWGCWC 3 cut(s) 182, 462, 1146
BbvI GCAGC 7 cut(s) 145, 261, 460, 463, 739, 1080, 1641
BccI CCATC 5 cut(s) 92, 424, 734, 1105, 1736
BceAI ACGGC 1 cut(s) 1408
BcgI CGANNNNNNTGC 2 cut(s) 1039, 1073
BciT130I CCWGG 1 cut(s) 1657
BciVI GTATCC 1 cut(s) 1069
BclI TGATCA 1 cut(s) 1389
BcnI CCSGG 1 cut(s) 140
BcoDI GTCTC 4 cut(s) 759, 1041, 1310, 1629
BcuI ACTAGT 2 cut(s) 1030, 1366
BfaI CTAG 7 cut(s) 444, 756, 1031, 1367, 1407, 1470, 1692
BfmI CTRYAG 1 cut(s) 1379
BfuI GTATCC 1 cut(s) 1069
BglI GCCNNNNNGGC 1 cut(s) 1299
BglII AGATCT 1 cut(s) 544
BisI GCNGC 7 cut(s) 159, 250, 474, 477, 753, 1094, 1630
BlsI GCNGC 7 cut(s) 160, 251, 475, 478, 754, 1095, 1631
Bme1390I CCNGG 2 cut(s) 140, 1657
Bme18I GGWCC 1 cut(s) 565
BmeT110I CYCGRG 1 cut(s) 78
BmgT120I GGNCC 4 cut(s) 44, 45, 565, 977
BmiI GGNNCC 6 cut(s) 45, 46, 280, 355, 962, 978
BmrFI CCNGG 2 cut(s) 140, 1657
BmrI ACTGGG 1 cut(s) 216
BmsI GCATC 5 cut(s) 210, 354, 789, 1393, 1512
BmuI ACTGGG 1 cut(s) 216
BoxI GACNNNNGTC 1 cut(s) 656
BpiI GAAGAC 1 cut(s) 1460
Bpu10I CCTNAGC 2 cut(s) 150, 1556
BpuEI CTTGAG 2 cut(s) 1570, 1570
BpuMI CCSGG 1 cut(s) 140
BsaBI GATNNNNATC 1 cut(s) 1713
BsaJI CCNNGG 1 cut(s) 48
Bsc4I CCNNNNNNNGG 3 cut(s) 379, 422, 1402
Bse118I RCCGGY 1 cut(s) 28
Bse1I ACTGG 8 cut(s) 211, 510, 661, 711, 1101, 1206, 1303, 1396
Bse21I CCTNAGG 1 cut(s) 283
Bse3DI GCAATG 2 cut(s) 94, 1200
Bse8I GATNNNNATC 1 cut(s) 1713
BseBI CCWGG 1 cut(s) 1657
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 5 cut(s) 460, 1097, 1490, 1566, 1738
BseJI GATNNNNATC 1 cut(s) 1713
BseLI CCNNNNNNNGG 3 cut(s) 379, 422, 1402
BseMI GCAATG 2 cut(s) 94, 1200
BseMII CTCAG 4 cut(s) 297, 1298, 1570, 1610
BseNI ACTGG 8 cut(s) 211, 510, 661, 711, 1101, 1206, 1303, 1396
BseRI GAGGAG 1 cut(s) 777
BseSI GKGCMC 1 cut(s) 48
BseXI GCAGC 7 cut(s) 145, 261, 460, 463, 739, 1080, 1641
BseYI CCCAGC 1 cut(s) 1239
Bsh1285I CGRYCG 2 cut(s) 1049, 1717
BshFI GGCC 5 cut(s) 46, 143, 979, 1293, 1696
BshNI GGYRCC 2 cut(s) 278, 353
BsiEI CGRYCG 2 cut(s) 1049, 1717
BsiHKAI GWGCWC 3 cut(s) 182, 462, 1146
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 2 cut(s) 29, 140
BslFI GGGAC 1 cut(s) 91
BslI CCNNNNNNNGG 3 cut(s) 379, 422, 1402
BsmAI GTCTC 4 cut(s) 759, 1041, 1310, 1629
BsmFI GGGAC 1 cut(s) 91
BsmI GAATGC 2 cut(s) 1302, 1425
BsnI GGCC 5 cut(s) 46, 143, 979, 1293, 1696
BsoBI CYCGRG 1 cut(s) 78
Bsp120I GGGCCC 1 cut(s) 44
Bsp1286I GDGCHC 4 cut(s) 48, 182, 462, 1146
Bsp1407I TGTACA 1 cut(s) 886
Bsp143I GATC 6 cut(s) 544, 1046, 1389, 1663, 1714, 1719
BspANI GGCC 5 cut(s) 46, 143, 979, 1293, 1696
BspCNI CTCAG 4 cut(s) 296, 1299, 1569, 1611
BspLI GGNNCC 6 cut(s) 45, 46, 280, 355, 962, 978
BspPI GGATC 2 cut(s) 1658, 1727
BspQI GCTCTTC 1 cut(s) 1230
BspT107I GGYRCC 2 cut(s) 278, 353
BsrDI GCAATG 2 cut(s) 94, 1200
BsrFI RCCGGY 1 cut(s) 28
BsrGI TGTACA 1 cut(s) 886
BsrI ACTGG 8 cut(s) 211, 510, 661, 711, 1101, 1206, 1303, 1396
BssAI RCCGGY 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 6 cut(s) 544, 1046, 1389, 1663, 1714, 1719
BssT1I CCWWGG 1 cut(s) 48
Bst2UI CCWGG 1 cut(s) 1657
Bst4CI ACNGT 5 cut(s) 22, 105, 239, 1134, 1487
Bst6I CTCTTC 3 cut(s) 754, 852, 1230
BstAUI TGTACA 1 cut(s) 886
BstC8I GCNNGC 3 cut(s) 156, 199, 624
BstDEI CTNAG 5 cut(s) 150, 283, 1307, 1556, 1619
BstENI CCTNNNNNAGG 1 cut(s) 377
BstF5I GGATG 5 cut(s) 460, 1097, 1490, 1566, 1738
BstHHI GCGC 1 cut(s) 1122
BstKTI GATC 6 cut(s) 547, 1049, 1392, 1666, 1717, 1722
BstMAI GTCTC 4 cut(s) 759, 1041, 1310, 1629
BstMBI GATC 6 cut(s) 544, 1046, 1389, 1663, 1714, 1719
BstMCI CGRYCG 2 cut(s) 1049, 1717
BstMWI GCNNNNNNNGC 4 cut(s) 198, 1299, 1337, 1693
BstNI CCWGG 1 cut(s) 1657
BstNSI RCATGY 1 cut(s) 138
BstPAI GACNNNNGTC 1 cut(s) 656
BstSCI CCNGG 2 cut(s) 138, 1655
BstSFI CTRYAG 1 cut(s) 1379
BstSLI GKGCMC 1 cut(s) 48
BstV1I GCAGC 7 cut(s) 145, 261, 460, 463, 739, 1080, 1641
BstV2I GAAGAC 1 cut(s) 1460
BstX2I RGATCY 2 cut(s) 544, 1663
BstYI RGATCY 2 cut(s) 544, 1663
Bsu36I CCTNAGG 1 cut(s) 283
BsuI GTATCC 1 cut(s) 1069
BsuRI GGCC 5 cut(s) 46, 143, 979, 1293, 1696
BtsCI GGATG 5 cut(s) 460, 1097, 1490, 1566, 1738
BtsI GCAGTG 1 cut(s) 931
BtsIMutI CAGTG 6 cut(s) 27, 204, 517, 931, 1062, 1108
Cac8I GCNNGC 3 cut(s) 156, 199, 624
CaiI CAGNNNCTG 2 cut(s) 1346, 1387
CfoI GCGC 1 cut(s) 1122
Cfr10I RCCGGY 1 cut(s) 28
Cfr13I GGNCC 4 cut(s) 44, 45, 565, 977
Csp6I GTAC 3 cut(s) 126, 279, 887
CviAII CATG 6 cut(s) 135, 253, 911, 1286, 1328, 1597
CviQI GTAC 3 cut(s) 126, 279, 887
DdeI CTNAG 5 cut(s) 150, 283, 1307, 1556, 1619
DpnI GATC 6 cut(s) 546, 1048, 1391, 1665, 1716, 1721
DpnII GATC 6 cut(s) 544, 1046, 1389, 1663, 1714, 1719
DraI TTTAAA 3 cut(s) 399, 825, 1363
EaeI YGGCCR 2 cut(s) 141, 1291
Eam1104I CTCTTC 3 cut(s) 754, 852, 1230
EarI CTCTTC 3 cut(s) 754, 852, 1230
Ecl136II GAGCTC 3 cut(s) 180, 460, 1144
Eco130I CCWWGG 1 cut(s) 48
Eco24I GRGCYC 4 cut(s) 48, 182, 462, 1146
Eco47I GGWCC 1 cut(s) 565
Eco53kI GAGCTC 3 cut(s) 180, 460, 1144
Eco57I CTGAAG 1 cut(s) 1368
Eco81I CCTNAGG 1 cut(s) 283
Eco88I CYCGRG 1 cut(s) 78
EcoICRI GAGCTC 3 cut(s) 180, 460, 1144
EcoNI CCTNNNNNAGG 1 cut(s) 377
EcoO109I RGGNCCY 2 cut(s) 44, 977
EcoRI GAATTC 1 cut(s) 9
EcoRII CCWGG 1 cut(s) 1655
EcoT14I CCWWGG 1 cut(s) 48
EcoT38I GRGCYC 4 cut(s) 48, 182, 462, 1146
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 6 cut(s) 138, 256, 914, 1289, 1331, 1600
FalI AAGNNNNNCTT 4 cut(s) 1509, 1541, 1532, 1564
FaqI GGGAC 1 cut(s) 91
FatI CATG 6 cut(s) 134, 252, 910, 1285, 1327, 1596
FauNDI CATATG 2 cut(s) 693, 1068
FbaI TGATCA 1 cut(s) 1389
Fnu4HI GCNGC 7 cut(s) 159, 250, 474, 477, 753, 1094, 1630
FokI GGATG 5 cut(s) 467, 1084, 1477, 1573, 1745
FriOI GRGCYC 4 cut(s) 48, 182, 462, 1146
Fsp4HI GCNGC 7 cut(s) 159, 250, 474, 477, 753, 1094, 1630
FspBI CTAG 7 cut(s) 444, 756, 1031, 1367, 1407, 1470, 1692
GlaI GCGC 1 cut(s) 1121
GluI GCNGC 7 cut(s) 159, 250, 474, 477, 753, 1094, 1630
GsaI CCCAGC 1 cut(s) 1243
HaeIII GGCC 5 cut(s) 46, 143, 979, 1293, 1696
HapII CCGG 2 cut(s) 29, 140
HhaI GCGC 1 cut(s) 1122
Hin1II CATG 6 cut(s) 138, 256, 914, 1289, 1331, 1600
Hin6I GCGC 1 cut(s) 1120
HinP1I GCGC 1 cut(s) 1120
HincII GTYRAC 3 cut(s) 666, 1428, 1750
HindII GTYRAC 3 cut(s) 666, 1428, 1750
HindIII AAGCTT 3 cut(s) 872, 915, 1350
HinfI GANTC 2 cut(s) 1570, 1681
HpaII CCGG 2 cut(s) 29, 140
Hpy166II GTNNAC 4 cut(s) 666, 889, 1428, 1750
Hpy8I GTNNAC 4 cut(s) 666, 889, 1428, 1750
HpyAV CCTTC 2 cut(s) 211, 990
HpyCH4III ACNGT 5 cut(s) 22, 105, 239, 1134, 1487
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 8 cut(s) 161, 335, 367, 430, 691, 776, 802, 1331
HpyF10VI GCNNNNNNNGC 4 cut(s) 198, 1299, 1337, 1693
HpyF3I CTNAG 5 cut(s) 150, 283, 1307, 1556, 1619
HpySE526I ACGT 1 cut(s) 516
Hsp92II CATG 6 cut(s) 138, 256, 914, 1289, 1331, 1600
HspAI GCGC 1 cut(s) 1120
KpnI GGTACC 1 cut(s) 282
Ksp22I TGATCA 1 cut(s) 1389
Kzo9I GATC 6 cut(s) 544, 1046, 1389, 1663, 1714, 1719
LguI GCTCTTC 1 cut(s) 1230
LmnI GCTCC 1 cut(s) 465
Lsp1109I GCAGC 7 cut(s) 145, 261, 460, 463, 739, 1080, 1641
LweI GCATC 5 cut(s) 210, 354, 789, 1393, 1512
MaeI CTAG 7 cut(s) 444, 756, 1031, 1367, 1407, 1470, 1692
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 5 cut(s) 512, 595, 1041, 1208, 1409
MalI GATC 6 cut(s) 546, 1048, 1391, 1665, 1716, 1721
MboI GATC 6 cut(s) 544, 1046, 1389, 1663, 1714, 1719
MboII GAAGA 9 cut(s) 503, 592, 644, 771, 869, 1186, 1217, 1465, 1523
MfeI CAATTG 2 cut(s) 342, 644
MflI RGATCY 2 cut(s) 544, 1663
MhlI GDGCHC 4 cut(s) 48, 182, 462, 1146
MlsI TGGCCA 1 cut(s) 1293
MluNI TGGCCA 1 cut(s) 1293
MlyI GAGTC 2 cut(s) 1579, 1690
MmeI TCCRAC 2 cut(s) 678, 1225
Mox20I TGGCCA 1 cut(s) 1293
MroXI GAANNNNTTC 2 cut(s) 535, 611
MscI TGGCCA 1 cut(s) 1293
MslI CAYNNNNRTG 3 cut(s) 20, 1031, 1186
Msp20I TGGCCA 1 cut(s) 1293
MspI CCGG 2 cut(s) 29, 140
MspR9I CCNGG 2 cut(s) 140, 1657
MunI CAATTG 2 cut(s) 342, 644
Mva1269I GAATGC 2 cut(s) 1302, 1425
MvaI CCWGG 1 cut(s) 1657
MwoI GCNNNNNNNGC 4 cut(s) 198, 1299, 1337, 1693
NciI CCSGG 1 cut(s) 140
NdeI CATATG 2 cut(s) 693, 1068
NdeII GATC 6 cut(s) 544, 1046, 1389, 1663, 1714, 1719
NlaIII CATG 6 cut(s) 138, 256, 914, 1289, 1331, 1600
NlaIV GGNNCC 6 cut(s) 45, 46, 280, 355, 962, 978
NmeAIII GCCGAG 1 cut(s) 1658
NmuCI GTSAC 3 cut(s) 512, 1041, 1409
NspI RCATGY 1 cut(s) 138
OliI CACNNNNGTG 2 cut(s) 20, 1031
PaeR7I CTCGAG 1 cut(s) 78
PciSI GCTCTTC 1 cut(s) 1230
PctI GAATGC 2 cut(s) 1302, 1425
PdmI GAANNNNTTC 2 cut(s) 535, 611
PkrI GCNGC 7 cut(s) 160, 251, 475, 478, 754, 1095, 1631
Ple19I CGATCG 2 cut(s) 1049, 1717
PleI GAGTC 2 cut(s) 1578, 1689
PpsI GAGTC 2 cut(s) 1578, 1689
PshAI GACNNNNGTC 1 cut(s) 656
Psp124BI GAGCTC 3 cut(s) 182, 462, 1146
Psp6I CCWGG 1 cut(s) 1655
PspFI CCCAGC 1 cut(s) 1239
PspGI CCWGG 1 cut(s) 1655
PspN4I GGNNCC 6 cut(s) 45, 46, 280, 355, 962, 978
PspOMI GGGCCC 1 cut(s) 44
PspPI GGNCC 4 cut(s) 44, 45, 565, 977
PstNI CAGNNNCTG 2 cut(s) 1346, 1387
PsuI RGATCY 2 cut(s) 544, 1663
PvuI CGATCG 2 cut(s) 1049, 1717
RsaI GTAC 3 cut(s) 127, 280, 888
RsaNI GTAC 3 cut(s) 126, 279, 887
RseI CAYNNNNRTG 3 cut(s) 20, 1031, 1186
SacI GAGCTC 3 cut(s) 182, 462, 1146
SapI GCTCTTC 1 cut(s) 1230
SatI GCNGC 7 cut(s) 159, 250, 474, 477, 753, 1094, 1630
Sau3AI GATC 6 cut(s) 544, 1046, 1389, 1663, 1714, 1719
Sau96I GGNCC 4 cut(s) 44, 45, 565, 977
SchI GAGTC 2 cut(s) 1579, 1690
ScrFI CCNGG 2 cut(s) 140, 1657
SduI GDGCHC 4 cut(s) 48, 182, 462, 1146
SfaNI GCATC 5 cut(s) 210, 354, 789, 1393, 1512
SfcI CTRYAG 1 cut(s) 1379
Sfr274I CTCGAG 1 cut(s) 78
SinI GGWCC 1 cut(s) 565
SlaI CTCGAG 1 cut(s) 78
SmiMI CAYNNNNRTG 3 cut(s) 20, 1031, 1186
SmlI CTYRAG 3 cut(s) 78, 1549, 1585
SmoI CTYRAG 3 cut(s) 78, 1549, 1585
SpeI ACTAGT 2 cut(s) 1030, 1366
SspMI CTAG 7 cut(s) 444, 756, 1031, 1367, 1407, 1470, 1692
SstI GAGCTC 3 cut(s) 182, 462, 1146
StyD4I CCNGG 2 cut(s) 138, 1655
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 5 cut(s) 22, 105, 239, 1134, 1487
TaiI ACGT 1 cut(s) 519
TaqI TCGA 2 cut(s) 79, 1049
TatI WGTACW 2 cut(s) 125, 886
TscAI CASTG 6 cut(s) 27, 211, 517, 931, 1062, 1108
TseFI GTSAC 3 cut(s) 512, 1041, 1409
TseI GCWGC 7 cut(s) 158, 249, 473, 476, 752, 1093, 1629
Tsp45I GTSAC 3 cut(s) 512, 1041, 1409
TspDTI ATGAA 7 cut(s) 927, 951, 981, 1187, 1217, 1479, 1524
TspRI CASTG 6 cut(s) 27, 211, 517, 931, 1062, 1108
VpaK11BI GGWCC 1 cut(s) 565
XagI CCTNNNNNAGG 1 cut(s) 377
XapI RAATTY 3 cut(s) 9, 259, 1258
XbaI TCTAGA 2 cut(s) 443, 1469
XceI RCATGY 1 cut(s) 138
XcmI CCANNNNNNNNNTGG 2 cut(s) 917, 1674
XhoI CTCGAG 1 cut(s) 78
XmnI GAANNNNTTC 2 cut(s) 535, 611
XspI CTAG 7 cut(s) 444, 756, 1031, 1367, 1407, 1470, 1692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.