Rh6AG184400

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000458
Physical Location & Seq
Reverse (-)
32404090 .. 32412093
8004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG184400.1

Sequence Viewer

Length: 1758 bp
ATGCCGTCTCGGGGTAACCTTGTCTGCTGTTTCTTCATCCCTCTCATCAATCGGGTTTCAGTTGTTGTTGGAAACATTTCTGCTACGAAACTTGTCCATAACGAGTCAAATTCAGTTTTTGGTAATGGGTGTGATGAACTGCCCCCAAGAGCAAATGGTATTACAACTCCATCCAATTGCTTCTCTAAGCAGGCAGCTGTTGCTAAGGTTTCAGCAGTAAGCTCTCTTTTGGGTAAAGCTAGCTCGATTGGATTTGGGAAGGCAATGGAAATTTTGGACAGTTTTGGCAGTGGCATCACAAATTGGGACCCCACTTTAAATTCTGAGGTGCAAATAAAAGGGAATACAATTTCAATTTTGGCTTTTGAAGTTGCAAACTCAGTTGTCAAGGGTGCGAAGCTAATGCAGTCCCTTTCAGAAGATGAGATCAGATATTTAAAAGACGTGCTGCTACCATCCAAAGGGGTGCAAAGTTTAATATCTACATCTATGGATGAGCTCCTCAGAATTGCCGCTGCTGACAAGAGAGAAGAGTTGAGTACTTTTTCGAGAGATGTTGTACGTTTTGGAAATCGTACTAAGGATCCGACATGGCACAACTTGGATCGCTATTTTAGGAAGACTGGCTTGGAAGTTACTGAACAAACTTATTTAAAGAAGCAAGCAGAGAGAGGGATGGAACATTTGATAACTCTAGTTCAGTCAACAGCAGAACTATGTCATGAGTTGCATTCGTTGGATAGATTGGAACGAGAATATCGGCATAAGCTTGAAGAAGATAGTTCAGTTACTGTTAAAAGAGGAGACAACCTTGCAATTTTAAACGCAGATATAAAGAATCAAATGAAGCTTGTAAGCAGCTTAAAGAAAAAATCACTGTGGTCAAAGACTTTTGAAGAGGTTATGCCGAAGCTTGTAGACATAGTGCAATTATTACACTTCAAAATTCATGAAGCTTTTGGAAGTACTGATGATGACGAACCAGTGGAACGTTCTGAGAGGAACTGTAAAAGGTTGGGGCCTGCTGGTACTTCATTACACTATGCGCATATTATTACACAAATTAACACACTTGTGTCTCGATCAAGGTCAGTGCCTCAAAGTGTGATAGATACTCTGTATGGGGGACTGCCATGCAGTGTAAAGTCAACTCTGCGCTCAAAATTTCAGTTAGTTGAGCTCACCATTCCTCAAACCAAAGCTGAAATGAAGCAAACTTTGGATTGGCTTGTTCCCATCGCCATTAACACAACCAAAGCTCTTCATGGCTTTGGATGGGTTGGAGAGTGGGCAAATACAGGGTTTGAGGCAAGCTGGCATACTGGCTTGACTAGCATTCCGAGGATTGGGACTCTACACCATGCCGATGAGAAAAAAACTGAAGCTTATATATGTGAACTAGTAGTCTTGCTACATTATCTGATCAGCCAGGCGGGGGTTGGCCATGGTGGAATGCCATCGTCACCTGTTGAAACCCGCAGCCAGAGGATGAATGAGTTAACAGCTGAAGATCAAGAGATGCTTAAGGATGTGGGTATAAACAGCATCAGACTAGGAATGAGTAAAAGTCATAATTTTGACGCAGGAAAGGCCAGCAGGTTGAGCAGACACCATAGGCTGAGTATGAGTAGCAGAGACTTCCTAGCAAAAGATAAACTTCCCATTGAGAATCTGTTGTCTAGGCCCATAGACTCAGATATAACCTGGACCAAGTTCTTGGATGTCATTGATGGAGTTGACACAATGCGCAGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

585

Amino Acids

65.16

Weight (kDa)

8.28

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 119 - 175 1.5e-20 Domain of unknown function (DUF3475)
DUF668 PF05003 338 - 419 6.6e-21 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34320 AT1G34320
fragaria_vesca FvH4_2g12340 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_5g36070 FvH4_5g36100 FvH4_5g36130 FvH4_5g36140
malus_domestica MD05G1039900.v1.1 MD10G1045400.v1.1 MD10G1045600.v1.1 MD10G1045700.v1.1 MD10G1045800.v1.1 MD12G1057900.v1.1
prunus_persica Prupe.1G525500_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1
pyrus_communis pycom05g03250 pycom10g03180 pycom10g03190 pycom15g33960
rosa_chinensis RchiOBHm_Chr3g0484671 RchiOBHm_Chr6g0272351 RchiOBHm_Chr6g0272361 RchiOBHm_Chr7g0237881
rosa_laevigata RLG00000000952 RLG00000000960 RLG00000001025 RLG00000008689 RLG00000010609 RLG00000013679 RLG00000013680
rosa_multiflora Rmu_sc0001339.1_g000015 Rmu_sc0002877.1_g000020 Rmu_sc0009379.1_g000003 Rmu_ssc0000199.1_g000001 Rmu_ssc0000321.1_g000001
rosa_roxburghii Rroxscaffold_1G00039160 Rroxscaffold_3G00223500 Rroxscaffold_3G00223670 Rroxscaffold_7G00196370 Rroxscaffold_7G00196380
rosa_rugosa Rorug06G0068200 Rorug06G0068300 Rorug06G0068400 Rorug07G0302100 Rorug07G0308600 Rorug07G0309200.1
rosa_samantha Rh3AG263300 Rh3DG292000 Rh6AG113700 Rh6AG184400 Rh6AG184500 Rh6BG187800 Rh6BG187900 Rh6CG185900 Rh6DG177600 Rh6DG177700 Rh7AG458500 Rh7AG464200 Rh7AG464500 Rh7BG429200 Rh7BG434200 Rh7CG476400 Rh7CG481600 Rh7CG482700 Rh7DG445700 Rh7DG450300 Rh7DG451400
rosa_wichuraiana Rw0G019450 Rw6G015900 Rw6G015910 Rw6G015940 Rw6G015950 Rw7G037980 Rw7G038410 Rw7G038470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 1047, 1748
Acc36I ACCTGC 2 cut(s) 1587, 1740
AccI GTMKAC 1 cut(s) 918
AciI CCGC 3 cut(s) 513, 1433, 1477
AclI AACGTT 1 cut(s) 991
AclWI GGATC 3 cut(s) 578, 591, 612
AcoI YGGCCR 1 cut(s) 1441
AcsI RAATTY 5 cut(s) 109, 270, 319, 945, 1163
AcuI CTGAAG 2 cut(s) 1401, 1527
AfaI GTAC 5 cut(s) 541, 561, 577, 967, 1030
AfiI CCNNNNNNNGG 4 cut(s) 11, 461, 1346, 1435
AflII CTTAAG 1 cut(s) 1523
AgsI TTSAA 6 cut(s) 354, 368, 773, 896, 943, 1472
AhlI ACTAGT 1 cut(s) 1399
AjiI CACGTC 1 cut(s) 445
AjnI CCWGG 2 cut(s) 1428, 1703
AjuI GAANNNNNNNTTGG 4 cut(s) 611, 643, 1202, 1234
AleI CACNNNNGTG 1 cut(s) 1073
Alw21I GWGCWC 2 cut(s) 501, 1182
Alw26I GTCTC 4 cut(s) 12, 798, 1083, 1629
AlwI GGATC 3 cut(s) 578, 591, 612
AlwNI CAGNNNCTG 1 cut(s) 791
Ama87I CYCGRG 1 cut(s) 9
AoxI GGCC 4 cut(s) 1019, 1441, 1590, 1682
ApeKI GCWGC 5 cut(s) 194, 448, 515, 858, 1479
ApoI RAATTY 5 cut(s) 109, 270, 319, 945, 1163
Asp700I GAANNNNTTC 1 cut(s) 76
AspLEI GCGC 3 cut(s) 1048, 1158, 1749
AspS9I GGNCC 4 cut(s) 307, 1019, 1683, 1707
AsuHPI GGTGA 2 cut(s) 1174, 1455
AsuNHI GCTAGC 1 cut(s) 239
AvaI CYCGRG 1 cut(s) 9
AvaII GGWCC 2 cut(s) 307, 1707
BalI TGGCCA 1 cut(s) 1443
BamHI GGATCC 1 cut(s) 583
BanII GRGCYC 2 cut(s) 501, 1182
BbsI GAAGAC 1 cut(s) 626
Bbv12I GWGCWC 2 cut(s) 501, 1182
BbvI GCAGC 5 cut(s) 206, 435, 502, 870, 1491
BccI CCATC 7 cut(s) 178, 463, 670, 1244, 1269, 1465, 1724
BcgI CGANNNNNNTGC 2 cut(s) 385, 419
BciT130I CCWGG 2 cut(s) 1430, 1705
BclI TGATCA 1 cut(s) 1422
BcoDI GTCTC 4 cut(s) 12, 798, 1083, 1629
BcuI ACTAGT 1 cut(s) 1399
BfaI CTAG 7 cut(s) 240, 695, 1332, 1400, 1553, 1643, 1680
BfrI CTTAAG 1 cut(s) 1523
BfuAI ACCTGC 2 cut(s) 1587, 1740
BisI GCNGC 6 cut(s) 195, 449, 513, 516, 859, 1480
BlsI GCNGC 6 cut(s) 196, 450, 514, 517, 860, 1481
BmcAI AGTACT 2 cut(s) 541, 967
Bme1390I CCNGG 2 cut(s) 1430, 1705
Bme18I GGWCC 2 cut(s) 307, 1707
BmeT110I CYCGRG 1 cut(s) 9
BmgBI CACGTC 1 cut(s) 445
BmgT120I GGNCC 4 cut(s) 307, 1019, 1683, 1707
BmiI GGNNCC 4 cut(s) 308, 309, 585, 1020
BmrFI CCNGG 2 cut(s) 1430, 1705
BmsI GCATC 3 cut(s) 303, 1509, 1554
BmtI GCTAGC 1 cut(s) 243
BpiI GAAGAC 1 cut(s) 626
Bpu10I CCTNAGC 1 cut(s) 204
BsaJI CCNNGG 2 cut(s) 1340, 1444
Bsc4I CCNNNNNNNGG 4 cut(s) 11, 461, 1346, 1435
Bse1I ACTGG 3 cut(s) 628, 983, 1327
Bse3DI GCAATG 1 cut(s) 270
BseBI CCWGG 2 cut(s) 1430, 1705
BseDI CCNNGG 2 cut(s) 1340, 1444
BseGI GGATG 9 cut(s) 36, 170, 455, 499, 681, 1280, 1494, 1534, 1726
BseLI CCNNNNNNNGG 4 cut(s) 11, 461, 1346, 1435
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 6 cut(s) 315, 393, 517, 987, 1610, 1707
BseNI ACTGG 3 cut(s) 628, 983, 1327
BseRI GAGGAG 2 cut(s) 491, 816
BseXI GCAGC 5 cut(s) 206, 435, 502, 870, 1491
BshFI GGCC 4 cut(s) 1021, 1443, 1592, 1684
BsiHKAI GWGCWC 2 cut(s) 501, 1182
BsiHKCI CYCGRG 1 cut(s) 9
BslFI GGGAC 4 cut(s) 320, 394, 1140, 1363
BslI CCNNNNNNNGG 4 cut(s) 11, 461, 1346, 1435
BsmAI GTCTC 4 cut(s) 12, 798, 1083, 1629
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 4 cut(s) 320, 394, 1140, 1363
BsmI GAATGC 3 cut(s) 730, 1335, 1458
BsnI GGCC 4 cut(s) 1021, 1443, 1592, 1684
BsoBI CYCGRG 1 cut(s) 9
Bsp1286I GDGCHC 2 cut(s) 501, 1182
Bsp143I GATC 6 cut(s) 426, 583, 604, 1082, 1422, 1510
Bsp19I CCATGG 1 cut(s) 1444
BspACI CCGC 3 cut(s) 513, 1433, 1477
BspANI GGCC 4 cut(s) 1021, 1443, 1592, 1684
BspCNI CTCAG 6 cut(s) 316, 392, 516, 988, 1611, 1706
BspHI TCATGA 2 cut(s) 721, 949
BspLI GGNNCC 4 cut(s) 308, 309, 585, 1020
BspMI ACCTGC 2 cut(s) 1587, 1740
BspOI GCTAGC 1 cut(s) 243
BspPI GGATC 3 cut(s) 578, 591, 612
BspQI GCTCTTC 1 cut(s) 1266
BspTI CTTAAG 1 cut(s) 1523
BsrDI GCAATG 1 cut(s) 270
BsrI ACTGG 3 cut(s) 628, 983, 1327
BssECI CCNNGG 2 cut(s) 1340, 1444
BssMI GATC 6 cut(s) 426, 583, 604, 1082, 1422, 1510
BssT1I CCWWGG 1 cut(s) 1444
Bst2UI CCWGG 2 cut(s) 1430, 1705
Bst4CI ACNGT 4 cut(s) 281, 793, 879, 1007
Bst6I CTCTTC 3 cut(s) 525, 891, 1266
BstAFI CTTAAG 1 cut(s) 1523
BstAPI GCANNNNNTGC 1 cut(s) 200
BstC8I GCNNGC 7 cut(s) 192, 241, 663, 1023, 1312, 1316, 1594
BstDEI CTNAG 9 cut(s) 186, 204, 324, 379, 503, 579, 996, 1619, 1693
BstDSI CCRYGG 1 cut(s) 1444
BstEII GGTNACC 1 cut(s) 14
BstF5I GGATG 9 cut(s) 36, 170, 455, 499, 681, 1280, 1494, 1534, 1726
BstHHI GCGC 3 cut(s) 1048, 1158, 1749
BstKTI GATC 6 cut(s) 429, 586, 607, 1085, 1425, 1513
BstMAI GTCTC 4 cut(s) 12, 798, 1083, 1629
BstMBI GATC 6 cut(s) 426, 583, 604, 1082, 1422, 1510
BstMWI GCNNNNNNNGC 4 cut(s) 200, 1332, 1589, 1602
BstNI CCWGG 2 cut(s) 1430, 1705
BstPI GGTNACC 1 cut(s) 14
BstSCI CCNGG 2 cut(s) 1428, 1703
BstV1I GCAGC 5 cut(s) 206, 435, 502, 870, 1491
BstV2I GAAGAC 1 cut(s) 626
BstX2I RGATCY 1 cut(s) 583
BstXI CCANNNNNNTGG 1 cut(s) 1717
BstYI RGATCY 1 cut(s) 583
BsuRI GGCC 4 cut(s) 1021, 1443, 1592, 1684
BtgI CCRYGG 1 cut(s) 1444
BtgZI GCGATG 1 cut(s) 1222
BtrI CACGTC 1 cut(s) 445
BtsCI GGATG 9 cut(s) 36, 170, 455, 499, 681, 1280, 1494, 1534, 1726
BtsI GCAGTG 2 cut(s) 295, 1144
BtsIMutI CAGTG 5 cut(s) 295, 875, 990, 1098, 1144
BveI ACCTGC 2 cut(s) 1587, 1740
Cac8I GCNNGC 7 cut(s) 192, 241, 663, 1023, 1312, 1316, 1594
CaiI CAGNNNCTG 1 cut(s) 791
CciI TCATGA 2 cut(s) 721, 949
CfoI GCGC 3 cut(s) 1048, 1158, 1749
Cfr13I GGNCC 4 cut(s) 307, 1019, 1683, 1707
CseI GACGC 1 cut(s) 1589
Csp6I GTAC 5 cut(s) 540, 560, 576, 966, 1029
CviAII CATG 7 cut(s) 591, 722, 950, 1134, 1265, 1361, 1445
CviQI GTAC 5 cut(s) 540, 560, 576, 966, 1029
DdeI CTNAG 9 cut(s) 186, 204, 324, 379, 503, 579, 996, 1619, 1693
DpnI GATC 6 cut(s) 428, 585, 606, 1084, 1424, 1512
DpnII GATC 6 cut(s) 426, 583, 604, 1082, 1422, 1510
DraI TTTAAA 4 cut(s) 318, 438, 654, 822
EaeI YGGCCR 1 cut(s) 1441
Eam1104I CTCTTC 3 cut(s) 525, 891, 1266
EarI CTCTTC 3 cut(s) 525, 891, 1266
Ecl136II GAGCTC 2 cut(s) 499, 1180
Eco130I CCWWGG 1 cut(s) 1444
Eco24I GRGCYC 2 cut(s) 501, 1182
Eco47I GGWCC 2 cut(s) 307, 1707
Eco53kI GAGCTC 2 cut(s) 499, 1180
Eco57I CTGAAG 2 cut(s) 1401, 1527
Eco88I CYCGRG 1 cut(s) 9
Eco91I GGTNACC 1 cut(s) 14
EcoICRI GAGCTC 2 cut(s) 499, 1180
EcoO109I RGGNCCY 2 cut(s) 307, 1019
EcoO65I GGTNACC 1 cut(s) 14
EcoRII CCWGG 2 cut(s) 1428, 1703
EcoT14I CCWWGG 1 cut(s) 1444
EcoT38I GRGCYC 2 cut(s) 501, 1182
ErhI CCWWGG 1 cut(s) 1444
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 7 cut(s) 594, 725, 953, 1137, 1268, 1364, 1448
FalI AAGNNNNNCTT 6 cut(s) 611, 643, 1506, 1538, 1641, 1673
FaqI GGGAC 4 cut(s) 320, 394, 1140, 1363
FatI CATG 7 cut(s) 590, 721, 949, 1133, 1264, 1360, 1444
FauI CCCGC 2 cut(s) 1426, 1484
FbaI TGATCA 1 cut(s) 1422
FblI GTMKAC 1 cut(s) 918
Fnu4HI GCNGC 6 cut(s) 195, 449, 513, 516, 859, 1480
FokI GGATG 9 cut(s) 23, 157, 442, 506, 688, 1287, 1501, 1541, 1733
FriOI GRGCYC 2 cut(s) 501, 1182
Fsp4HI GCNGC 6 cut(s) 195, 449, 513, 516, 859, 1480
FspAI RTGCGCAY 1 cut(s) 1047
FspBI CTAG 7 cut(s) 240, 695, 1332, 1400, 1553, 1643, 1680
FspI TGCGCA 2 cut(s) 1047, 1748
GlaI GCGC 3 cut(s) 1047, 1157, 1748
GluI GCNGC 6 cut(s) 195, 449, 513, 516, 859, 1480
HaeIII GGCC 4 cut(s) 1021, 1443, 1592, 1684
HgaI GACGC 1 cut(s) 1589
HhaI GCGC 3 cut(s) 1048, 1158, 1749
Hin1II CATG 7 cut(s) 594, 725, 953, 1137, 1268, 1364, 1448
Hin6I GCGC 3 cut(s) 1046, 1156, 1747
HinP1I GCGC 3 cut(s) 1046, 1156, 1747
HincII GTYRAC 4 cut(s) 705, 1149, 1500, 1738
HindII GTYRAC 4 cut(s) 705, 1149, 1500, 1738
HindIII AAGCTT 5 cut(s) 767, 848, 911, 954, 1383
HinfI GANTC 5 cut(s) 104, 838, 1351, 1669, 1691
HpaI GTTAAC 1 cut(s) 1500
HphI GGTGA 2 cut(s) 1174, 1455
Hpy166II GTNNAC 6 cut(s) 705, 919, 1149, 1397, 1500, 1738
Hpy188III TCNNGA 5 cut(s) 549, 722, 950, 1080, 1514
Hpy8I GTNNAC 6 cut(s) 705, 919, 1149, 1397, 1500, 1738
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 4 cut(s) 281, 793, 879, 1007
HpyCH4IV ACGT 3 cut(s) 444, 562, 991
HpyCH4V TGCA 8 cut(s) 331, 374, 406, 469, 730, 815, 928, 1137
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 1332, 1589, 1602
HpyF3I CTNAG 9 cut(s) 186, 204, 324, 379, 503, 579, 996, 1619, 1693
HpySE526I ACGT 3 cut(s) 444, 562, 991
Hsp92II CATG 7 cut(s) 594, 725, 953, 1137, 1268, 1364, 1448
HspAI GCGC 3 cut(s) 1046, 1156, 1747
KflI GGGWCCC 1 cut(s) 307
Ksp22I TGATCA 1 cut(s) 1422
KspAI GTTAAC 1 cut(s) 1500
Kzo9I GATC 6 cut(s) 426, 583, 604, 1082, 1422, 1510
LguI GCTCTTC 1 cut(s) 1266
LmnI GCTCC 1 cut(s) 504
Lsp1109I GCAGC 5 cut(s) 206, 435, 502, 870, 1491
LweI GCATC 3 cut(s) 303, 1509, 1554
MaeI CTAG 7 cut(s) 240, 695, 1332, 1400, 1553, 1643, 1680
MaeII ACGT 3 cut(s) 444, 562, 991
MaeIII GTNAC 4 cut(s) 14, 634, 787, 1461
MalI GATC 6 cut(s) 428, 585, 606, 1084, 1424, 1512
MboI GATC 6 cut(s) 426, 583, 604, 1082, 1422, 1510
MboII GAAGA 9 cut(s) 25, 431, 542, 631, 785, 788, 908, 1253, 1520
MfeI CAATTG 1 cut(s) 175
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 2 cut(s) 501, 1182
MlsI TGGCCA 1 cut(s) 1443
MluNI TGGCCA 1 cut(s) 1443
MlyI GAGTC 3 cut(s) 113, 1345, 1685
MmeI TCCRAC 4 cut(s) 49, 611, 717, 1261
Mox20I TGGCCA 1 cut(s) 1443
MroXI GAANNNNTTC 1 cut(s) 76
MscI TGGCCA 1 cut(s) 1443
MslI CAYNNNNRTG 2 cut(s) 1073, 1365
Msp20I TGGCCA 1 cut(s) 1443
MspA1I CMGCKG 3 cut(s) 197, 515, 1505
MspCI CTTAAG 1 cut(s) 1523
MspR9I CCNGG 2 cut(s) 1430, 1705
MunI CAATTG 1 cut(s) 175
Mva1269I GAATGC 3 cut(s) 730, 1335, 1458
MvaI CCWGG 2 cut(s) 1430, 1705
MwoI GCNNNNNNNGC 4 cut(s) 200, 1332, 1589, 1602
NcoI CCATGG 1 cut(s) 1444
NdeII GATC 6 cut(s) 426, 583, 604, 1082, 1422, 1510
NheI GCTAGC 1 cut(s) 239
NlaIII CATG 7 cut(s) 594, 725, 953, 1137, 1268, 1364, 1448
NlaIV GGNNCC 4 cut(s) 308, 309, 585, 1020
NmuCI GTSAC 1 cut(s) 1461
NsbI TGCGCA 2 cut(s) 1047, 1748
OliI CACNNNNGTG 1 cut(s) 1073
PagI TCATGA 2 cut(s) 721, 949
PciSI GCTCTTC 1 cut(s) 1266
PctI GAATGC 3 cut(s) 730, 1335, 1458
PdmI GAANNNNTTC 1 cut(s) 76
PfeI GAWTC 2 cut(s) 838, 1669
PkrI GCNGC 6 cut(s) 196, 450, 514, 517, 860, 1481
PleI GAGTC 3 cut(s) 112, 1345, 1685
PpsI GAGTC 3 cut(s) 112, 1345, 1685
PpuMI RGGWCCY 1 cut(s) 307
Psp124BI GAGCTC 2 cut(s) 501, 1182
Psp1406I AACGTT 1 cut(s) 991
Psp5II RGGWCCY 1 cut(s) 307
Psp6I CCWGG 2 cut(s) 1428, 1703
PspEI GGTNACC 1 cut(s) 14
PspGI CCWGG 2 cut(s) 1428, 1703
PspN4I GGNNCC 4 cut(s) 308, 309, 585, 1020
PspPI GGNCC 4 cut(s) 307, 1019, 1683, 1707
PspPPI RGGWCCY 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 791
PsuI RGATCY 1 cut(s) 583
PvuII CAGCTG 2 cut(s) 197, 1505
RsaI GTAC 5 cut(s) 541, 561, 577, 967, 1030
RsaNI GTAC 5 cut(s) 540, 560, 576, 966, 1029
RseI CAYNNNNRTG 2 cut(s) 1073, 1365
SacI GAGCTC 2 cut(s) 501, 1182
SapI GCTCTTC 1 cut(s) 1266
SatI GCNGC 6 cut(s) 195, 449, 513, 516, 859, 1480
Sau3AI GATC 6 cut(s) 426, 583, 604, 1082, 1422, 1510
Sau96I GGNCC 4 cut(s) 307, 1019, 1683, 1707
ScaI AGTACT 2 cut(s) 541, 967
SchI GAGTC 3 cut(s) 113, 1345, 1685
ScrFI CCNGG 2 cut(s) 1430, 1705
SduI GDGCHC 2 cut(s) 501, 1182
SfaNI GCATC 3 cut(s) 303, 1509, 1554
SinI GGWCC 2 cut(s) 307, 1707
SmiMI CAYNNNNRTG 2 cut(s) 1073, 1365
SmlI CTYRAG 1 cut(s) 1523
SmoI CTYRAG 1 cut(s) 1523
SpeI ACTAGT 1 cut(s) 1399
SsiI CCGC 3 cut(s) 513, 1433, 1477
SspMI CTAG 7 cut(s) 240, 695, 1332, 1400, 1553, 1643, 1680
SstI GAGCTC 2 cut(s) 501, 1182
StyD4I CCNGG 2 cut(s) 1428, 1703
StyI CCWWGG 1 cut(s) 1444
TaaI ACNGT 4 cut(s) 281, 793, 879, 1007
TaiI ACGT 3 cut(s) 447, 565, 994
TaqI TCGA 3 cut(s) 245, 548, 1081
TatI WGTACW 2 cut(s) 539, 965
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 2 cut(s) 838, 1669
TscAI CASTG 5 cut(s) 295, 882, 990, 1098, 1144
TseFI GTSAC 1 cut(s) 1461
TseI GCWGC 5 cut(s) 194, 448, 515, 858, 1479
Tsp45I GTSAC 1 cut(s) 1461
TspDTI ATGAA 9 cut(s) 25, 150, 860, 938, 966, 1023, 1223, 1253, 1505
TspRI CASTG 5 cut(s) 295, 882, 990, 1098, 1144
Vha464I CTTAAG 1 cut(s) 1523
VpaK11BI GGWCC 2 cut(s) 307, 1707
XapI RAATTY 5 cut(s) 109, 270, 319, 945, 1163
XcmI CCANNNNNNNNNTGG 1 cut(s) 1436
XmiI GTMKAC 1 cut(s) 918
XmnI GAANNNNTTC 1 cut(s) 76
XspI CTAG 7 cut(s) 240, 695, 1332, 1400, 1553, 1643, 1680
ZrmI AGTACT 2 cut(s) 541, 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.