RchiOBHm_Chr6g0272351

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000458
Physical Location & Seq
Reverse (-)
32594022 .. 32602834
8813 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24433

Sequence Viewer

Length: 1731 bp
ATGGGTGGGATTTTCAACAAGGTCAAACGGACGCCCAAAGTAGAAGATGTCACTGTAGCTATAAATTATCATCATGCTAGTAATGGGTGTGATGAACTGCCCCCAAGAGCAAATGGTATTACAACTCCATCCAATTGCTTCTCTAAGCAGGCAGCTGTTGCTAAGGTTTCAGCAGTAAGCTCTCTTTTGGGTAAAGCTAGCTCGATTGGATTTGGGAAGTTAATGGAAATTTTGGACAGTTTTGGCACTAGCATCACAAATTGGGACCCCACTTTAAATTCTGAGGTGCAAACAAAAGGGACTACAATTTCAATTTTGGCTTTTGAAGTTGCAAACTCAGTTGTCAAGGGTGCGAAGCTAATGCAGTCCCTTTCAGAAGATGAGATCAGATATTTAAAAGATGTGCTGCTACCATCCAAAGGGGTGCAAAGTTTAATATCTACAGATATGGATGAGCTCCTCAGAATTGCCGCTGCTGACAAGAGAGAAGAGTTGAGTACTTTTTCGAGAGATGTTGTACGTTTTGGAAATCATACTAAGGATCCGACATGGCACAACTTGGATCGCTATTTTAGGGAGACTGGCTTGAAAGTTACCGAACAAACTTATTTAAAGAAGCAAGCAGAGAGAGGGATGGAACATTTGATAACTCTAGTTCAGTCAACAGCAGAACTATGTCATGAGTTGCATTCGTTGGATAGATTGGAACGAGAATATCGGCATAAGCTTGAAGAAGATAGTTCAGTTACTGTTAAAAGAGGTAACCTTGTCTTTGGAGACAACCTTGCAATTTTAAACGCAGATATAAAGAATCAAATGAAGCTTGTAAACAGCTTAAAGAAAAAATCACTGTGGTCAAAGACTTTTGAAGAGGTTATGCCGAAGCTTGTAGACATTGTGCAATTATTACACTTCAAAATTCATGAAGCTTTTGGAAGTACTGATGATGACGAACCAGTGGAACGTTCTGAGAGGAACTGTAAAAGGTTGGGGTCTGCTGGTACTTCATTACACTATGCGCATATTATTACACAAATTAACACACTTGTGTCTCGATCAAGGTCAGTGCCTCAAAGTGTGATAGATACTCTGTATGAGGGACTGCCATGCAGTGTAAAGTCAACTCTGCGCTCAAAATTTCAGTTAGTTGAGCTCACCATTCCTCAAATCAAAGCTGAAATGAAGCAAACTTTGGATTGGCTTGTTCCCATCGCCATTAACACAACCAAAGCTCTTCATGGCTTTGGATGGGTTGGAGAGTGGGCAAATACAGGGTTTGAGGCAAGCTGGTATACTGGCTTGACTAGCATTCCGAGGATTGGGACTCTACACCATGCCGATGAGAAAAAAACTGAAGCTTATATATGTGAACTAGTAGTCTTGCTACATTATCTGATCAGCCAGGCGGGGGTTGGCCATGGTGGAATGCCATCGTCACCTGTTGAAACCCGCAGCCAGAGGACGAATGAGTTAACAGCTGAAGATCAAGAGATGCTTAAGGATGTGGGTATAAACAGCATCAGACTAGGAATGAGTAAAAGTCATAATTTTGACGCAGGAAAGGCCAGCAGGTTGAGCAGACACCATAGGCTGAGTAAGAGTAGCAGAGACTTCCTAGCAAAAGATAAACTTCCCATTGAGAATCTGTTGTCTAGGCCCATAGACTCCGATATAACCTGGACCAAGTTCTTGGATGTCATTGATGGAGTTGACACAACGTGCAGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

576

Amino Acids

64.36

Weight (kDa)

7.33

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 105 - 161 3.7e-22 Domain of unknown function (DUF3475)
DUF668 PF05003 329 - 410 1.5e-21 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34320 AT1G34320
fragaria_vesca FvH4_2g12340 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_5g36070 FvH4_5g36100 FvH4_5g36130 FvH4_5g36140
malus_domestica MD05G1039900.v1.1 MD10G1045400.v1.1 MD10G1045600.v1.1 MD10G1045700.v1.1 MD10G1045800.v1.1 MD12G1057900.v1.1
prunus_persica Prupe.1G525500_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1
pyrus_communis pycom05g03250 pycom10g03180 pycom10g03190 pycom15g33960
rosa_chinensis RchiOBHm_Chr3g0484671 RchiOBHm_Chr6g0272351 RchiOBHm_Chr6g0272361 RchiOBHm_Chr7g0237881
rosa_laevigata RLG00000000952 RLG00000000960 RLG00000001025 RLG00000008689 RLG00000010609 RLG00000013679 RLG00000013680
rosa_multiflora Rmu_sc0001339.1_g000015 Rmu_sc0002877.1_g000020 Rmu_sc0009379.1_g000003 Rmu_ssc0000199.1_g000001 Rmu_ssc0000321.1_g000001
rosa_roxburghii Rroxscaffold_1G00039160 Rroxscaffold_3G00223500 Rroxscaffold_3G00223670 Rroxscaffold_7G00196370 Rroxscaffold_7G00196380
rosa_rugosa Rorug06G0068200 Rorug06G0068300 Rorug06G0068400 Rorug07G0302100 Rorug07G0308600 Rorug07G0309200.1
rosa_samantha Rh3AG263300 Rh3DG292000 Rh6AG113700 Rh6AG184400 Rh6AG184500 Rh6BG187800 Rh6BG187900 Rh6CG185900 Rh6DG177600 Rh6DG177700 Rh7AG458500 Rh7AG464200 Rh7AG464500 Rh7BG429200 Rh7BG434200 Rh7CG476400 Rh7CG481600 Rh7CG482700 Rh7DG445700 Rh7DG450300 Rh7DG451400
rosa_wichuraiana Rw0G019450 Rw6G015900 Rw6G015910 Rw6G015940 Rw6G015950 Rw7G037980 Rw7G038410 Rw7G038470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1020
Acc36I ACCTGC 2 cut(s) 1560, 1713
AccI GTMKAC 2 cut(s) 891, 1292
AciI CCGC 3 cut(s) 471, 1406, 1450
AclI AACGTT 1 cut(s) 964
AclWI GGATC 3 cut(s) 536, 549, 570
AcoI YGGCCR 1 cut(s) 1414
AcsI RAATTY 4 cut(s) 228, 277, 918, 1136
AcuI CTGAAG 2 cut(s) 1374, 1500
AcyI GRCGYC 1 cut(s) 32
AdeI CACNNNGTG 1 cut(s) 1719
AfaI GTAC 4 cut(s) 499, 519, 940, 1003
AfiI CCNNNNNNNGG 3 cut(s) 419, 1319, 1408
AflII CTTAAG 1 cut(s) 1496
AgsI TTSAA 8 cut(s) 16, 312, 326, 589, 731, 869, 916, 1445
AhlI ACTAGT 1 cut(s) 1372
AjnI CCWGG 2 cut(s) 1401, 1676
AjuI GAANNNNNNNTTGG 2 cut(s) 1175, 1207
AleI CACNNNNGTG 1 cut(s) 1046
Alw21I GWGCWC 2 cut(s) 459, 1155
Alw26I GTCTC 4 cut(s) 572, 771, 1056, 1602
AlwI GGATC 3 cut(s) 536, 549, 570
AlwNI CAGNNNCTG 1 cut(s) 749
AoxI GGCC 3 cut(s) 1414, 1563, 1655
ApeKI GCWGC 4 cut(s) 152, 406, 473, 1452
ApoI RAATTY 4 cut(s) 228, 277, 918, 1136
AspLEI GCGC 2 cut(s) 1021, 1131
AspS9I GGNCC 3 cut(s) 265, 1656, 1680
AsuHPI GGTGA 2 cut(s) 1147, 1428
AsuNHI GCTAGC 1 cut(s) 197
AvaII GGWCC 2 cut(s) 265, 1680
BalI TGGCCA 1 cut(s) 1416
BamHI GGATCC 1 cut(s) 541
BanII GRGCYC 2 cut(s) 459, 1155
Bbv12I GWGCWC 2 cut(s) 459, 1155
BbvI GCAGC 4 cut(s) 164, 393, 460, 1464
BccI CCATC 7 cut(s) 136, 421, 628, 1217, 1242, 1438, 1697
BcgI CGANNNNNNTGC 2 cut(s) 343, 377
BciT130I CCWGG 2 cut(s) 1403, 1678
BclI TGATCA 1 cut(s) 1395
BcoDI GTCTC 4 cut(s) 572, 771, 1056, 1602
BcuI ACTAGT 1 cut(s) 1372
BfaI CTAG 9 cut(s) 78, 198, 249, 653, 1305, 1373, 1526, 1616, 1653
BfmI CTRYAG 2 cut(s) 54, 441
BfrI CTTAAG 1 cut(s) 1496
BfuAI ACCTGC 2 cut(s) 1560, 1713
BisI GCNGC 5 cut(s) 153, 407, 471, 474, 1453
BlsI GCNGC 5 cut(s) 154, 408, 472, 475, 1454
BmcAI AGTACT 2 cut(s) 499, 940
Bme1390I CCNGG 2 cut(s) 1403, 1678
Bme18I GGWCC 2 cut(s) 265, 1680
BmgT120I GGNCC 3 cut(s) 265, 1656, 1680
BmiI GGNNCC 3 cut(s) 266, 267, 543
BmrFI CCNGG 2 cut(s) 1403, 1678
BmsI GCATC 3 cut(s) 261, 1482, 1527
BmtI GCTAGC 1 cut(s) 201
Bpu10I CCTNAGC 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 2 cut(s) 1313, 1417
Bsc4I CCNNNNNNNGG 3 cut(s) 419, 1319, 1408
Bse1I ACTGG 3 cut(s) 586, 956, 1300
BseBI CCWGG 2 cut(s) 1403, 1678
BseDI CCNNGG 2 cut(s) 1313, 1417
BseGI GGATG 7 cut(s) 128, 413, 457, 639, 1253, 1507, 1699
BseLI CCNNNNNNNGG 3 cut(s) 419, 1319, 1408
BseMII CTCAG 5 cut(s) 273, 351, 475, 960, 1583
BseNI ACTGG 3 cut(s) 586, 956, 1300
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 4 cut(s) 164, 393, 460, 1464
BshFI GGCC 3 cut(s) 1416, 1565, 1657
BsiHKAI GWGCWC 2 cut(s) 459, 1155
BslFI GGGAC 5 cut(s) 278, 313, 352, 1113, 1336
BslI CCNNNNNNNGG 3 cut(s) 419, 1319, 1408
BsmAI GTCTC 4 cut(s) 572, 771, 1056, 1602
BsmFI GGGAC 5 cut(s) 278, 313, 352, 1113, 1336
BsmI GAATGC 3 cut(s) 688, 1308, 1431
BsnI GGCC 3 cut(s) 1416, 1565, 1657
Bsp1286I GDGCHC 2 cut(s) 459, 1155
Bsp143I GATC 6 cut(s) 384, 541, 562, 1055, 1395, 1483
Bsp19I CCATGG 1 cut(s) 1417
BspACI CCGC 3 cut(s) 471, 1406, 1450
BspANI GGCC 3 cut(s) 1416, 1565, 1657
BspCNI CTCAG 5 cut(s) 274, 350, 474, 961, 1584
BspHI TCATGA 2 cut(s) 679, 922
BspLI GGNNCC 3 cut(s) 266, 267, 543
BspMI ACCTGC 2 cut(s) 1560, 1713
BspOI GCTAGC 1 cut(s) 201
BspPI GGATC 3 cut(s) 536, 549, 570
BspQI GCTCTTC 1 cut(s) 1239
BspTI CTTAAG 1 cut(s) 1496
BsrI ACTGG 3 cut(s) 586, 956, 1300
BssECI CCNNGG 2 cut(s) 1313, 1417
BssMI GATC 6 cut(s) 384, 541, 562, 1055, 1395, 1483
BssNAI GTATAC 1 cut(s) 1293
BssNI GRCGYC 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 1417
Bst1107I GTATAC 1 cut(s) 1293
Bst2UI CCWGG 2 cut(s) 1403, 1678
Bst4CI ACNGT 5 cut(s) 55, 239, 751, 852, 980
Bst6I CTCTTC 3 cut(s) 483, 864, 1239
BstACI GRCGYC 1 cut(s) 32
BstAFI CTTAAG 1 cut(s) 1496
BstAPI GCANNNNNTGC 1 cut(s) 158
BstC8I GCNNGC 5 cut(s) 150, 199, 621, 1285, 1567
BstDEI CTNAG 8 cut(s) 144, 162, 282, 337, 461, 537, 969, 1592
BstDSI CCRYGG 1 cut(s) 1417
BstEII GGTNACC 1 cut(s) 761
BstF5I GGATG 7 cut(s) 128, 413, 457, 639, 1253, 1507, 1699
BstHHI GCGC 2 cut(s) 1021, 1131
BstKTI GATC 6 cut(s) 387, 544, 565, 1058, 1398, 1486
BstMAI GTCTC 4 cut(s) 572, 771, 1056, 1602
BstMBI GATC 6 cut(s) 384, 541, 562, 1055, 1395, 1483
BstMWI GCNNNNNNNGC 4 cut(s) 158, 1305, 1562, 1575
BstNI CCWGG 2 cut(s) 1403, 1678
BstPI GGTNACC 1 cut(s) 761
BstSCI CCNGG 2 cut(s) 1401, 1676
BstSFI CTRYAG 2 cut(s) 54, 441
BstV1I GCAGC 4 cut(s) 164, 393, 460, 1464
BstX2I RGATCY 1 cut(s) 541
BstXI CCANNNNNNTGG 1 cut(s) 1690
BstYI RGATCY 1 cut(s) 541
BstZ17I GTATAC 1 cut(s) 1293
BsuRI GGCC 3 cut(s) 1416, 1565, 1657
BtgI CCRYGG 1 cut(s) 1417
BtgZI GCGATG 1 cut(s) 1195
BtsCI GGATG 7 cut(s) 128, 413, 457, 639, 1253, 1507, 1699
BtsI GCAGTG 1 cut(s) 1117
BtsIMutI CAGTG 5 cut(s) 51, 848, 963, 1071, 1117
BveI ACCTGC 2 cut(s) 1560, 1713
Cac8I GCNNGC 5 cut(s) 150, 199, 621, 1285, 1567
CaiI CAGNNNCTG 1 cut(s) 749
CciI TCATGA 2 cut(s) 679, 922
CfoI GCGC 2 cut(s) 1021, 1131
Cfr13I GGNCC 3 cut(s) 265, 1656, 1680
CseI GACGC 2 cut(s) 40, 1562
Csp6I GTAC 4 cut(s) 498, 518, 939, 1002
CviAII CATG 8 cut(s) 74, 549, 680, 923, 1107, 1238, 1334, 1418
CviQI GTAC 4 cut(s) 498, 518, 939, 1002
DdeI CTNAG 8 cut(s) 144, 162, 282, 337, 461, 537, 969, 1592
DpnI GATC 6 cut(s) 386, 543, 564, 1057, 1397, 1485
DpnII GATC 6 cut(s) 384, 541, 562, 1055, 1395, 1483
DraI TTTAAA 4 cut(s) 276, 396, 612, 795
DraIII CACNNNGTG 1 cut(s) 1719
EaeI YGGCCR 1 cut(s) 1414
Eam1104I CTCTTC 3 cut(s) 483, 864, 1239
EarI CTCTTC 3 cut(s) 483, 864, 1239
Ecl136II GAGCTC 2 cut(s) 457, 1153
Eco130I CCWWGG 1 cut(s) 1417
Eco24I GRGCYC 2 cut(s) 459, 1155
Eco47I GGWCC 2 cut(s) 265, 1680
Eco53kI GAGCTC 2 cut(s) 457, 1153
Eco57I CTGAAG 2 cut(s) 1374, 1500
Eco91I GGTNACC 1 cut(s) 761
EcoICRI GAGCTC 2 cut(s) 457, 1153
EcoO109I RGGNCCY 1 cut(s) 265
EcoO65I GGTNACC 1 cut(s) 761
EcoRII CCWGG 2 cut(s) 1401, 1676
EcoT14I CCWWGG 1 cut(s) 1417
EcoT38I GRGCYC 2 cut(s) 459, 1155
ErhI CCWWGG 1 cut(s) 1417
FaeI CATG 8 cut(s) 77, 552, 683, 926, 1110, 1241, 1337, 1421
FalI AAGNNNNNCTT 4 cut(s) 1479, 1511, 1614, 1646
FaqI GGGAC 5 cut(s) 278, 313, 352, 1113, 1336
FatI CATG 8 cut(s) 73, 548, 679, 922, 1106, 1237, 1333, 1417
FauI CCCGC 2 cut(s) 1399, 1457
FbaI TGATCA 1 cut(s) 1395
FblI GTMKAC 2 cut(s) 891, 1292
Fnu4HI GCNGC 5 cut(s) 153, 407, 471, 474, 1453
FokI GGATG 7 cut(s) 115, 400, 464, 646, 1260, 1514, 1706
FriOI GRGCYC 2 cut(s) 459, 1155
Fsp4HI GCNGC 5 cut(s) 153, 407, 471, 474, 1453
FspAI RTGCGCAY 1 cut(s) 1020
FspBI CTAG 9 cut(s) 78, 198, 249, 653, 1305, 1373, 1526, 1616, 1653
FspI TGCGCA 1 cut(s) 1020
GlaI GCGC 2 cut(s) 1020, 1130
GluI GCNGC 5 cut(s) 153, 407, 471, 474, 1453
HaeIII GGCC 3 cut(s) 1416, 1565, 1657
HgaI GACGC 2 cut(s) 40, 1562
HhaI GCGC 2 cut(s) 1021, 1131
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 8 cut(s) 77, 552, 683, 926, 1110, 1241, 1337, 1421
Hin6I GCGC 2 cut(s) 1019, 1129
HinP1I GCGC 2 cut(s) 1019, 1129
HincII GTYRAC 4 cut(s) 663, 1122, 1473, 1711
HindII GTYRAC 4 cut(s) 663, 1122, 1473, 1711
HindIII AAGCTT 5 cut(s) 725, 821, 884, 927, 1356
HinfI GANTC 4 cut(s) 811, 1324, 1642, 1664
HpaI GTTAAC 1 cut(s) 1473
HphI GGTGA 2 cut(s) 1147, 1428
Hpy166II GTNNAC 8 cut(s) 663, 829, 892, 1122, 1293, 1370, 1473, 1711
Hpy188III TCNNGA 5 cut(s) 507, 680, 923, 1053, 1487
Hpy8I GTNNAC 8 cut(s) 663, 829, 892, 1122, 1293, 1370, 1473, 1711
HpyCH4III ACNGT 5 cut(s) 55, 239, 751, 852, 980
HpyCH4IV ACGT 3 cut(s) 520, 964, 1718
HpyCH4V TGCA 9 cut(s) 289, 332, 364, 427, 688, 788, 901, 1110, 1722
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 1305, 1562, 1575
HpyF3I CTNAG 8 cut(s) 144, 162, 282, 337, 461, 537, 969, 1592
HpySE526I ACGT 3 cut(s) 520, 964, 1718
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 8 cut(s) 77, 552, 683, 926, 1110, 1241, 1337, 1421
HspAI GCGC 2 cut(s) 1019, 1129
KflI GGGWCCC 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 1395
KspAI GTTAAC 1 cut(s) 1473
Kzo9I GATC 6 cut(s) 384, 541, 562, 1055, 1395, 1483
LguI GCTCTTC 1 cut(s) 1239
LmnI GCTCC 1 cut(s) 462
Lsp1109I GCAGC 4 cut(s) 164, 393, 460, 1464
LweI GCATC 3 cut(s) 261, 1482, 1527
MaeI CTAG 9 cut(s) 78, 198, 249, 653, 1305, 1373, 1526, 1616, 1653
MaeII ACGT 3 cut(s) 520, 964, 1718
MaeIII GTNAC 5 cut(s) 49, 592, 745, 761, 1434
MalI GATC 6 cut(s) 386, 543, 564, 1057, 1397, 1485
MboI GATC 6 cut(s) 384, 541, 562, 1055, 1395, 1483
MboII GAAGA 8 cut(s) 56, 389, 500, 743, 746, 881, 1226, 1493
MfeI CAATTG 1 cut(s) 133
MflI RGATCY 1 cut(s) 541
MhlI GDGCHC 2 cut(s) 459, 1155
MlsI TGGCCA 1 cut(s) 1416
MluNI TGGCCA 1 cut(s) 1416
MlyI GAGTC 2 cut(s) 1318, 1658
MmeI TCCRAC 3 cut(s) 569, 675, 1234
Mox20I TGGCCA 1 cut(s) 1416
MscI TGGCCA 1 cut(s) 1416
MslI CAYNNNNRTG 2 cut(s) 1046, 1338
Msp20I TGGCCA 1 cut(s) 1416
MspA1I CMGCKG 3 cut(s) 155, 473, 1478
MspCI CTTAAG 1 cut(s) 1496
MspR9I CCNGG 2 cut(s) 1403, 1678
MunI CAATTG 1 cut(s) 133
Mva1269I GAATGC 3 cut(s) 688, 1308, 1431
MvaI CCWGG 2 cut(s) 1403, 1678
MwoI GCNNNNNNNGC 4 cut(s) 158, 1305, 1562, 1575
NcoI CCATGG 1 cut(s) 1417
NdeII GATC 6 cut(s) 384, 541, 562, 1055, 1395, 1483
NheI GCTAGC 1 cut(s) 197
NlaIII CATG 8 cut(s) 77, 552, 683, 926, 1110, 1241, 1337, 1421
NlaIV GGNNCC 3 cut(s) 266, 267, 543
NmuCI GTSAC 2 cut(s) 49, 1434
NsbI TGCGCA 1 cut(s) 1020
OliI CACNNNNGTG 1 cut(s) 1046
PagI TCATGA 2 cut(s) 679, 922
PciSI GCTCTTC 1 cut(s) 1239
PctI GAATGC 3 cut(s) 688, 1308, 1431
PfeI GAWTC 2 cut(s) 811, 1642
PkrI GCNGC 5 cut(s) 154, 408, 472, 475, 1454
PleI GAGTC 2 cut(s) 1318, 1658
PpsI GAGTC 2 cut(s) 1318, 1658
PpuMI RGGWCCY 1 cut(s) 265
Psp124BI GAGCTC 2 cut(s) 459, 1155
Psp1406I AACGTT 1 cut(s) 964
Psp5II RGGWCCY 1 cut(s) 265
Psp6I CCWGG 2 cut(s) 1401, 1676
PspEI GGTNACC 1 cut(s) 761
PspGI CCWGG 2 cut(s) 1401, 1676
PspN4I GGNNCC 3 cut(s) 266, 267, 543
PspPI GGNCC 3 cut(s) 265, 1656, 1680
PspPPI RGGWCCY 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 1 cut(s) 541
PvuII CAGCTG 2 cut(s) 155, 1478
RsaI GTAC 4 cut(s) 499, 519, 940, 1003
RsaNI GTAC 4 cut(s) 498, 518, 939, 1002
RseI CAYNNNNRTG 2 cut(s) 1046, 1338
SacI GAGCTC 2 cut(s) 459, 1155
SapI GCTCTTC 1 cut(s) 1239
SatI GCNGC 5 cut(s) 153, 407, 471, 474, 1453
Sau3AI GATC 6 cut(s) 384, 541, 562, 1055, 1395, 1483
Sau96I GGNCC 3 cut(s) 265, 1656, 1680
ScaI AGTACT 2 cut(s) 499, 940
SchI GAGTC 2 cut(s) 1318, 1658
ScrFI CCNGG 2 cut(s) 1403, 1678
SduI GDGCHC 2 cut(s) 459, 1155
SfaNI GCATC 3 cut(s) 261, 1482, 1527
SfcI CTRYAG 2 cut(s) 54, 441
SinI GGWCC 2 cut(s) 265, 1680
SmiMI CAYNNNNRTG 2 cut(s) 1046, 1338
SmlI CTYRAG 1 cut(s) 1496
SmoI CTYRAG 1 cut(s) 1496
SpeI ACTAGT 1 cut(s) 1372
SsiI CCGC 3 cut(s) 471, 1406, 1450
SspMI CTAG 9 cut(s) 78, 198, 249, 653, 1305, 1373, 1526, 1616, 1653
SstI GAGCTC 2 cut(s) 459, 1155
StyD4I CCNGG 2 cut(s) 1401, 1676
StyI CCWWGG 1 cut(s) 1417
TaaI ACNGT 5 cut(s) 55, 239, 751, 852, 980
TaiI ACGT 3 cut(s) 523, 967, 1721
TaqI TCGA 3 cut(s) 203, 506, 1054
TatI WGTACW 2 cut(s) 497, 938
TauI GCSGC 1 cut(s) 473
TfiI GAWTC 2 cut(s) 811, 1642
TscAI CASTG 5 cut(s) 58, 855, 963, 1071, 1117
TseFI GTSAC 2 cut(s) 49, 1434
TseI GCWGC 4 cut(s) 152, 406, 473, 1452
Tsp45I GTSAC 2 cut(s) 49, 1434
TspDTI ATGAA 7 cut(s) 108, 833, 911, 939, 996, 1196, 1226
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 5 cut(s) 58, 855, 963, 1071, 1117
Vha464I CTTAAG 1 cut(s) 1496
VpaK11BI GGWCC 2 cut(s) 265, 1680
XapI RAATTY 4 cut(s) 228, 277, 918, 1136
XcmI CCANNNNNNNNNTGG 1 cut(s) 1409
XmiI GTMKAC 2 cut(s) 891, 1292
XspI CTAG 9 cut(s) 78, 198, 249, 653, 1305, 1373, 1526, 1616, 1653
ZrmI AGTACT 2 cut(s) 499, 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.