Domain of unknown function (DUF3475)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1501 |
| Acc65I | GGTACC | 1 cut(s) | 250 |
| AccB1I | GGYRCC | 1 cut(s) | 250 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 467 |
| AccI | GTMKAC | 1 cut(s) | 69 |
| AccII | CGCG | 1 cut(s) | 1267 |
| AciI | CCGC | 5 cut(s) | 821, 919, 1267, 1292, 1393 |
| AclI | AACGTT | 1 cut(s) | 1388 |
| AclWI | GGATC | 6 cut(s) | 54, 145, 734, 768, 1874, 1887 |
| AcoI | YGGCCR | 1 cut(s) | 121 |
| AcsI | RAATTY | 7 cut(s) | 163, 451, 504, 553, 628, 1047, 1793 |
| AfaI | GTAC | 4 cut(s) | 252, 394, 624, 949 |
| AjnI | CCWGG | 2 cut(s) | 1294, 1811 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1725, 1757 |
| AluBI | AGCT | 7 cut(s) | 372, 691, 866, 925, 1310, 1373, 1430 |
| AluI | AGCT | 7 cut(s) | 372, 691, 866, 925, 1310, 1373, 1430 |
| Alw21I | GWGCWC | 2 cut(s) | 47, 693 |
| Alw26I | GTCTC | 5 cut(s) | 210, 230, 818, 1242, 1517 |
| AlwI | GGATC | 6 cut(s) | 54, 145, 734, 768, 1874, 1887 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1133 |
| Ama87I | CYCGRG | 1 cut(s) | 703 |
| AoxI | GGCC | 4 cut(s) | 121, 382, 400, 1898 |
| ApeKI | GCWGC | 3 cut(s) | 668, 671, 835 |
| ApoI | RAATTY | 7 cut(s) | 163, 451, 504, 553, 628, 1047, 1793 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 1313, 1345, 1457, 1489, 1913, 1945 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1740 |
| Asp718I | GGTACC | 1 cut(s) | 250 |
| AspLEI | GCGC | 1 cut(s) | 1317 |
| AspS9I | GGNCC | 5 cut(s) | 306, 382, 1041, 1473, 1809 |
| AsuC2I | CCSGG | 2 cut(s) | 386, 1773 |
| AsuHPI | GGTGA | 3 cut(s) | 496, 1580, 1701 |
| AsuII | TTCGAA | 1 cut(s) | 1978 |
| AvaI | CYCGRG | 1 cut(s) | 703 |
| AvaII | GGWCC | 4 cut(s) | 306, 1041, 1473, 1809 |
| AxyI | CCTNAGG | 1 cut(s) | 478 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1738, 1771 |
| BalI | TGGCCA | 1 cut(s) | 123 |
| BamHI | GGATCC | 1 cut(s) | 1879 |
| BanI | GGYRCC | 1 cut(s) | 250 |
| BanII | GRGCYC | 3 cut(s) | 264, 693, 1290 |
| BauI | CACGAG | 1 cut(s) | 141 |
| BbsI | GAAGAC | 2 cut(s) | 942, 1734 |
| Bbv12I | GWGCWC | 2 cut(s) | 47, 693 |
| BbvCI | CCTCAGC | 1 cut(s) | 1514 |
| BbvI | GCAGC | 3 cut(s) | 655, 658, 847 |
| BccI | CCATC | 5 cut(s) | 132, 187, 619, 1599, 1909 |
| BciT130I | CCWGG | 2 cut(s) | 1296, 1813 |
| BciVI | GTATCC | 1 cut(s) | 1264 |
| BcnI | CCSGG | 2 cut(s) | 386, 1773 |
| BcoDI | GTCTC | 5 cut(s) | 210, 230, 818, 1242, 1517 |
| BfaI | CTAG | 2 cut(s) | 129, 1400 |
| BfmI | CTRYAG | 2 cut(s) | 66, 1325 |
| BfuAI | ACCTGC | 1 cut(s) | 1501 |
| BfuI | GTATCC | 1 cut(s) | 1264 |
| BglII | AGATCT | 2 cut(s) | 326, 1631 |
| BisI | GCNGC | 3 cut(s) | 669, 672, 836 |
| BlpI | GCTNAGC | 1 cut(s) | 1835 |
| BlsI | GCNGC | 3 cut(s) | 670, 673, 837 |
| BmcAI | AGTACT | 1 cut(s) | 949 |
| Bme1390I | CCNGG | 4 cut(s) | 386, 1296, 1773, 1813 |
| Bme18I | GGWCC | 4 cut(s) | 306, 1041, 1473, 1809 |
| BmeT110I | CYCGRG | 1 cut(s) | 703 |
| BmgT120I | GGNCC | 5 cut(s) | 306, 382, 1041, 1473, 1809 |
| BmiI | GGNNCC | 5 cut(s) | 252, 1043, 1289, 1474, 1881 |
| BmrFI | CCNGG | 4 cut(s) | 386, 1296, 1773, 1813 |
| BmrI | ACTGGG | 2 cut(s) | 179, 1479 |
| BmsI | GCATC | 2 cut(s) | 808, 822 |
| BmuI | ACTGGG | 2 cut(s) | 179, 1479 |
| BpiI | GAAGAC | 2 cut(s) | 942, 1734 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 675, 707 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1514 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1835 |
| Bpu14I | TTCGAA | 1 cut(s) | 1978 |
| BpuEI | CTTGAG | 3 cut(s) | 928, 1000, 1789 |
| BpuMI | CCSGG | 2 cut(s) | 386, 1773 |
| BsaAI | YACGTR | 1 cut(s) | 1750 |
| BsaI | GGTCTC | 1 cut(s) | 210 |
| BsaJI | CCNNGG | 4 cut(s) | 403, 433, 891, 1772 |
| BsaWI | WCCGGW | 1 cut(s) | 1935 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 699, 729, 1373, 1403 |
| Bse118I | RCCGGY | 1 cut(s) | 1830 |
| Bse1I | ACTGG | 4 cut(s) | 185, 1474, 1578, 1953 |
| Bse21I | CCTNAGG | 1 cut(s) | 478 |
| Bse3DI | GCAATG | 1 cut(s) | 1407 |
| BseBI | CCWGG | 2 cut(s) | 1296, 1813 |
| BseDI | CCNNGG | 4 cut(s) | 403, 433, 891, 1772 |
| BseGI | GGATG | 6 cut(s) | 45, 124, 179, 655, 1901, 1957 |
| BseMI | GCAATG | 1 cut(s) | 1407 |
| BseMII | CTCAG | 7 cut(s) | 492, 650, 758, 1169, 1505, 1545, 1826 |
| BseNI | ACTGG | 4 cut(s) | 185, 1474, 1578, 1953 |
| BseRI | GAGGAG | 2 cut(s) | 70, 975 |
| BseXI | GCAGC | 3 cut(s) | 655, 658, 847 |
| BseYI | CCCAGC | 1 cut(s) | 1446 |
| BsgI | GTGCAG | 1 cut(s) | 1514 |
| Bsh1236I | CGCG | 1 cut(s) | 1267 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1936 |
| BshFI | GGCC | 4 cut(s) | 123, 384, 402, 1900 |
| BshNI | GGYRCC | 1 cut(s) | 250 |
| BsiEI | CGRYCG | 1 cut(s) | 1936 |
| BsiHKAI | GWGCWC | 2 cut(s) | 47, 693 |
| BsiHKCI | CYCGRG | 1 cut(s) | 703 |
| BsiSI | CCGG | 5 cut(s) | 233, 385, 1772, 1831, 1936 |
| BslFI | GGGAC | 2 cut(s) | 550, 1027 |
| BsmAI | GTCTC | 5 cut(s) | 210, 230, 818, 1242, 1517 |
| BsmFI | GGGAC | 2 cut(s) | 550, 1027 |
| BsmI | GAATGC | 1 cut(s) | 105 |
| BsnI | GGCC | 4 cut(s) | 123, 384, 402, 1900 |
| Bso31I | GGTCTC | 1 cut(s) | 210 |
| BsoBI | CYCGRG | 1 cut(s) | 703 |
| Bsp119I | TTCGAA | 1 cut(s) | 1978 |
| Bsp1286I | GDGCHC | 4 cut(s) | 47, 264, 693, 1290 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1835 |
| BspACI | CCGC | 5 cut(s) | 821, 919, 1267, 1292, 1393 |
| BspANI | GGCC | 4 cut(s) | 123, 384, 402, 1900 |
| BspCNI | CTCAG | 7 cut(s) | 491, 651, 757, 1168, 1506, 1546, 1827 |
| BspFNI | CGCG | 1 cut(s) | 1267 |
| BspHI | TCATGA | 2 cut(s) | 877, 1108 |
| BspLI | GGNNCC | 5 cut(s) | 252, 1043, 1289, 1474, 1881 |
| BspMI | ACCTGC | 1 cut(s) | 1501 |
| BspPI | GGATC | 6 cut(s) | 54, 145, 734, 768, 1874, 1887 |
| BspQI | GCTCTTC | 2 cut(s) | 681, 1341 |
| BspT104I | TTCGAA | 1 cut(s) | 1978 |
| BspT107I | GGYRCC | 1 cut(s) | 250 |
| BspTNI | GGTCTC | 1 cut(s) | 210 |
| BsrDI | GCAATG | 1 cut(s) | 1407 |
| BsrFI | RCCGGY | 1 cut(s) | 1830 |
| BsrI | ACTGG | 4 cut(s) | 185, 1474, 1578, 1953 |
| BssAI | RCCGGY | 1 cut(s) | 1830 |
| BssECI | CCNNGG | 4 cut(s) | 403, 433, 891, 1772 |
| BssSI | CACGAG | 1 cut(s) | 141 |
| BssT1I | CCWWGG | 3 cut(s) | 403, 433, 891 |
| Bst2BI | CACGAG | 1 cut(s) | 141 |
| Bst2UI | CCWGG | 2 cut(s) | 1296, 1813 |
| Bst4CI | ACNGT | 6 cut(s) | 67, 538, 829, 1329, 1622, 1723 |
| Bst6I | CTCTTC | 4 cut(s) | 681, 989, 1050, 1341 |
| BstBAI | YACGTR | 1 cut(s) | 1750 |
| BstBI | TTCGAA | 1 cut(s) | 1978 |
| BstC8I | GCNNGC | 3 cut(s) | 345, 400, 1823 |
| BstDEI | CTNAG | 9 cut(s) | 339, 478, 573, 659, 744, 1155, 1514, 1554, 1835 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 407, 572, 1146 |
| BstF5I | GGATG | 6 cut(s) | 45, 124, 179, 655, 1901, 1957 |
| BstFNI | CGCG | 1 cut(s) | 1267 |
| BstHHI | GCGC | 1 cut(s) | 1317 |
| BstMAI | GTCTC | 5 cut(s) | 210, 230, 818, 1242, 1517 |
| BstMCI | CGRYCG | 1 cut(s) | 1936 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 395, 1190, 1831, 1845 |
| BstNI | CCWGG | 2 cut(s) | 1296, 1813 |
| BstNSI | RCATGY | 2 cut(s) | 890, 1013 |
| BstSCI | CCNGG | 4 cut(s) | 384, 1294, 1771, 1811 |
| BstSFI | CTRYAG | 2 cut(s) | 66, 1325 |
| BstSNI | TACGTA | 1 cut(s) | 1750 |
| BstUI | CGCG | 1 cut(s) | 1267 |
| BstV1I | GCAGC | 3 cut(s) | 655, 658, 847 |
| BstV2I | GAAGAC | 2 cut(s) | 942, 1734 |
| BstX2I | RGATCY | 6 cut(s) | 59, 137, 326, 739, 1631, 1879 |
| BstYI | RGATCY | 6 cut(s) | 59, 137, 326, 739, 1631, 1879 |
| Bsu36I | CCTNAGG | 1 cut(s) | 478 |
| BsuI | GTATCC | 1 cut(s) | 1264 |
| BsuRI | GGCC | 4 cut(s) | 123, 384, 402, 1900 |
| BtsCI | GGATG | 6 cut(s) | 45, 124, 179, 655, 1901, 1957 |
| BtsI | GCAGTG | 3 cut(s) | 420, 472, 1526 |
| BtsIMutI | CAGTG | 8 cut(s) | 63, 420, 472, 752, 834, 1467, 1526, 1946 |
| BveI | ACCTGC | 1 cut(s) | 1501 |
| Cac8I | GCNNGC | 3 cut(s) | 345, 400, 1823 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1133 |
| CciI | TCATGA | 2 cut(s) | 877, 1108 |
| CfoI | GCGC | 1 cut(s) | 1317 |
| Cfr10I | RCCGGY | 1 cut(s) | 1830 |
| Cfr13I | GGNCC | 5 cut(s) | 306, 382, 1041, 1473, 1809 |
| CsiI | ACCWGGT | 1 cut(s) | 1811 |
| Csp6I | GTAC | 4 cut(s) | 251, 393, 623, 948 |
| CviAII | CATG | 8 cut(s) | 445, 878, 887, 1010, 1063, 1109, 1436, 1535 |
| CviQI | GTAC | 4 cut(s) | 251, 393, 623, 948 |
| DdeI | CTNAG | 9 cut(s) | 339, 478, 573, 659, 744, 1155, 1514, 1554, 1835 |
| EaeI | YGGCCR | 1 cut(s) | 121 |
| Eam1104I | CTCTTC | 4 cut(s) | 681, 989, 1050, 1341 |
| EarI | CTCTTC | 4 cut(s) | 681, 989, 1050, 1341 |
| EciI | GGCGGA | 1 cut(s) | 934 |
| Ecl136II | GAGCTC | 1 cut(s) | 691 |
| Eco105I | TACGTA | 1 cut(s) | 1750 |
| Eco130I | CCWWGG | 3 cut(s) | 403, 433, 891 |
| Eco24I | GRGCYC | 3 cut(s) | 264, 693, 1290 |
| Eco31I | GGTCTC | 1 cut(s) | 210 |
| Eco32I | GATATC | 1 cut(s) | 636 |
| Eco47I | GGWCC | 4 cut(s) | 306, 1041, 1473, 1809 |
| Eco53kI | GAGCTC | 1 cut(s) | 691 |
| Eco81I | CCTNAGG | 1 cut(s) | 478 |
| Eco88I | CYCGRG | 1 cut(s) | 703 |
| EcoICRI | GAGCTC | 1 cut(s) | 691 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 407, 572, 1146 |
| EcoO109I | RGGNCCY | 1 cut(s) | 306 |
| EcoRI | GAATTC | 1 cut(s) | 163 |
| EcoRII | CCWGG | 2 cut(s) | 1294, 1811 |
| EcoRV | GATATC | 1 cut(s) | 636 |
| EcoT14I | CCWWGG | 3 cut(s) | 403, 433, 891 |
| EcoT38I | GRGCYC | 3 cut(s) | 264, 693, 1290 |
| ErhI | CCWWGG | 3 cut(s) | 403, 433, 891 |
| FaeI | CATG | 8 cut(s) | 448, 881, 890, 1013, 1066, 1112, 1439, 1538 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1725, 1757 |
| FaqI | GGGAC | 2 cut(s) | 550, 1027 |
| FatI | CATG | 8 cut(s) | 444, 877, 886, 1009, 1062, 1108, 1435, 1534 |
| FauI | CCCGC | 2 cut(s) | 1260, 1299 |
| FauNDI | CATATG | 1 cut(s) | 1098 |
| FblI | GTMKAC | 1 cut(s) | 69 |
| Fnu4HI | GCNGC | 3 cut(s) | 669, 672, 836 |
| FokI | GGATG | 6 cut(s) | 52, 111, 166, 662, 1888, 1964 |
| FriOI | GRGCYC | 3 cut(s) | 264, 693, 1290 |
| Fsp4HI | GCNGC | 3 cut(s) | 669, 672, 836 |
| FspBI | CTAG | 2 cut(s) | 129, 1400 |
| GlaI | GCGC | 1 cut(s) | 1316 |
| GluI | GCNGC | 3 cut(s) | 669, 672, 836 |
| GsaI | CCCAGC | 1 cut(s) | 1450 |
| HaeIII | GGCC | 4 cut(s) | 123, 384, 402, 1900 |
| HapII | CCGG | 5 cut(s) | 233, 385, 1772, 1831, 1936 |
| HhaI | GCGC | 1 cut(s) | 1317 |
| Hin1II | CATG | 8 cut(s) | 448, 881, 890, 1013, 1066, 1112, 1439, 1538 |
| Hin6I | GCGC | 1 cut(s) | 1315 |
| HinP1I | GCGC | 1 cut(s) | 1315 |
| HincII | GTYRAC | 2 cut(s) | 280, 1600 |
| HindII | GTYRAC | 2 cut(s) | 280, 1600 |
| HindIII | AAGCTT | 1 cut(s) | 923 |
| HinfI | GANTC | 7 cut(s) | 175, 289, 457, 997, 1105, 1556, 1786 |
| HpaII | CCGG | 5 cut(s) | 233, 385, 1772, 1831, 1936 |
| HphI | GGTGA | 3 cut(s) | 496, 1580, 1701 |
| Hpy166II | GTNNAC | 5 cut(s) | 70, 280, 1600, 1868, 1969 |
| Hpy188I | TCNGA | 5 cut(s) | 364, 616, 787, 1158, 1477 |
| Hpy8I | GTNNAC | 5 cut(s) | 70, 280, 1600, 1868, 1969 |
| Hpy99I | CGWCG | 1 cut(s) | 287 |
| HpyAV | CCTTC | 8 cut(s) | 188, 403, 611, 805, 1266, 1336, 1663, 1870 |
| HpyCH4III | ACNGT | 6 cut(s) | 67, 538, 829, 1329, 1622, 1723 |
| HpyCH4IV | ACGT | 4 cut(s) | 848, 1388, 1749, 1870 |
| HpyCH4V | TGCA | 7 cut(s) | 562, 835, 974, 1127, 1198, 1205, 1531 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 395, 1190, 1831, 1845 |
| HpyF3I | CTNAG | 9 cut(s) | 339, 478, 573, 659, 744, 1155, 1514, 1554, 1835 |
| HpySE526I | ACGT | 4 cut(s) | 848, 1388, 1749, 1870 |
| Hsp92II | CATG | 8 cut(s) | 448, 881, 890, 1013, 1066, 1112, 1439, 1538 |
| HspAI | GCGC | 1 cut(s) | 1315 |
| KpnI | GGTACC | 1 cut(s) | 254 |
| LguI | GCTCTTC | 2 cut(s) | 681, 1341 |
| LmnI | GCTCC | 5 cut(s) | 50, 377, 1293, 1661, 1938 |
| Lsp1109I | GCAGC | 3 cut(s) | 655, 658, 847 |
| LweI | GCATC | 2 cut(s) | 808, 822 |
| MabI | ACCWGGT | 1 cut(s) | 1811 |
| MaeI | CTAG | 2 cut(s) | 129, 1400 |
| MaeII | ACGT | 4 cut(s) | 848, 1388, 1749, 1870 |
| MaeIII | GTNAC | 2 cut(s) | 484, 882 |
| MboII | GAAGA | 9 cut(s) | 698, 827, 947, 1006, 1036, 1067, 1220, 1358, 1739 |
| MfeI | CAATTG | 3 cut(s) | 209, 806, 839 |
| MflI | RGATCY | 6 cut(s) | 59, 137, 326, 739, 1631, 1879 |
| MhlI | GDGCHC | 4 cut(s) | 47, 264, 693, 1290 |
| MlsI | TGGCCA | 1 cut(s) | 123 |
| MluNI | TGGCCA | 1 cut(s) | 123 |
| MlyI | GAGTC | 4 cut(s) | 169, 1006, 1565, 1795 |
| MmeI | TCCRAC | 2 cut(s) | 1432, 1594 |
| Mox20I | TGGCCA | 1 cut(s) | 123 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1740 |
| MscI | TGGCCA | 1 cut(s) | 123 |
| MseI | TTAA | 4 cut(s) | 557, 1368, 1641, 1718 |
| Msp20I | TGGCCA | 1 cut(s) | 123 |
| MspA1I | CMGCKG | 1 cut(s) | 866 |
| MspI | CCGG | 5 cut(s) | 233, 385, 1772, 1831, 1936 |
| MspR9I | CCNGG | 4 cut(s) | 386, 1296, 1773, 1813 |
| MunI | CAATTG | 3 cut(s) | 209, 806, 839 |
| Mva1269I | GAATGC | 1 cut(s) | 105 |
| MvaI | CCWGG | 2 cut(s) | 1296, 1813 |
| MvnI | CGCG | 1 cut(s) | 1267 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 395, 1190, 1831, 1845 |
| NciI | CCSGG | 2 cut(s) | 386, 1773 |
| NdeI | CATATG | 1 cut(s) | 1098 |
| NlaIII | CATG | 8 cut(s) | 448, 881, 890, 1013, 1066, 1112, 1439, 1538 |
| NlaIV | GGNNCC | 5 cut(s) | 252, 1043, 1289, 1474, 1881 |
| NmuCI | GTSAC | 1 cut(s) | 484 |
| NspI | RCATGY | 2 cut(s) | 890, 1013 |
| NspV | TTCGAA | 1 cut(s) | 1978 |
| PagI | TCATGA | 2 cut(s) | 877, 1108 |
| PciSI | GCTCTTC | 2 cut(s) | 681, 1341 |
| PctI | GAATGC | 1 cut(s) | 105 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1740 |
| PfeI | GAWTC | 3 cut(s) | 289, 457, 1105 |
| PflFI | GACNNNGTC | 1 cut(s) | 74 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 467 |
| PkrI | GCNGC | 3 cut(s) | 670, 673, 837 |
| PleI | GAGTC | 4 cut(s) | 169, 1005, 1564, 1794 |
| PpsI | GAGTC | 4 cut(s) | 169, 1005, 1564, 1794 |
| Ppu21I | YACGTR | 1 cut(s) | 1750 |
| PpuMI | RGGWCCY | 1 cut(s) | 306 |
| Psp124BI | GAGCTC | 1 cut(s) | 693 |
| Psp1406I | AACGTT | 1 cut(s) | 1388 |
| Psp5II | RGGWCCY | 1 cut(s) | 306 |
| Psp6I | CCWGG | 2 cut(s) | 1294, 1811 |
| PspFI | CCCAGC | 1 cut(s) | 1446 |
| PspGI | CCWGG | 2 cut(s) | 1294, 1811 |
| PspN4I | GGNNCC | 5 cut(s) | 252, 1043, 1289, 1474, 1881 |
| PspPI | GGNCC | 5 cut(s) | 306, 382, 1041, 1473, 1809 |
| PspPPI | RGGWCCY | 1 cut(s) | 306 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1133 |
| PsuI | RGATCY | 6 cut(s) | 59, 137, 326, 739, 1631, 1879 |
| PsyI | GACNNNGTC | 1 cut(s) | 74 |
| PvuII | CAGCTG | 1 cut(s) | 866 |
| RsaI | GTAC | 4 cut(s) | 252, 394, 624, 949 |
| RsaNI | GTAC | 4 cut(s) | 251, 393, 623, 948 |
| SacI | GAGCTC | 1 cut(s) | 693 |
| SapI | GCTCTTC | 2 cut(s) | 681, 1341 |
| SaqAI | TTAA | 4 cut(s) | 557, 1368, 1641, 1718 |
| SatI | GCNGC | 3 cut(s) | 669, 672, 836 |
| Sau96I | GGNCC | 5 cut(s) | 306, 382, 1041, 1473, 1809 |
| ScaI | AGTACT | 1 cut(s) | 949 |
| SchI | GAGTC | 4 cut(s) | 169, 1006, 1565, 1795 |
| ScrFI | CCNGG | 4 cut(s) | 386, 1296, 1773, 1813 |
| SduI | GDGCHC | 4 cut(s) | 47, 264, 693, 1290 |
| SexAI | ACCWGGT | 1 cut(s) | 1811 |
| SfaNI | GCATC | 2 cut(s) | 808, 822 |
| SfcI | CTRYAG | 2 cut(s) | 66, 1325 |
| SfuI | TTCGAA | 1 cut(s) | 1978 |
| SinI | GGWCC | 4 cut(s) | 306, 1041, 1473, 1809 |
| SmlI | CTYRAG | 3 cut(s) | 943, 979, 1804 |
| SmoI | CTYRAG | 3 cut(s) | 943, 979, 1804 |
| SnaBI | TACGTA | 1 cut(s) | 1750 |
| SsiI | CCGC | 5 cut(s) | 821, 919, 1267, 1292, 1393 |
| SspI | AATATT | 1 cut(s) | 1210 |
| SspMI | CTAG | 2 cut(s) | 129, 1400 |
| SstI | GAGCTC | 1 cut(s) | 693 |
| StyD4I | CCNGG | 4 cut(s) | 384, 1294, 1771, 1811 |
| StyI | CCWWGG | 3 cut(s) | 403, 433, 891 |
| TaaI | ACNGT | 6 cut(s) | 67, 538, 829, 1329, 1622, 1723 |
| TaiI | ACGT | 4 cut(s) | 851, 1391, 1752, 1873 |
| TaqI | TCGA | 7 cut(s) | 173, 302, 1077, 1240, 1744, 1932, 1978 |
| TatI | WGTACW | 1 cut(s) | 947 |
| TfiI | GAWTC | 3 cut(s) | 289, 457, 1105 |
| Tru1I | TTAA | 4 cut(s) | 557, 1368, 1641, 1718 |
| Tru9I | TTAA | 4 cut(s) | 557, 1368, 1641, 1718 |
| TscAI | CASTG | 8 cut(s) | 70, 420, 472, 752, 834, 1474, 1533, 1953 |
| TseFI | GTSAC | 1 cut(s) | 484 |
| TseI | GCWGC | 3 cut(s) | 668, 671, 835 |
| Tsp45I | GTSAC | 1 cut(s) | 484 |
| TspDTI | ATGAA | 7 cut(s) | 17, 666, 1076, 1097, 1125, 1496, 1703 |
| TspGWI | ACGGA | 1 cut(s) | 1897 |
| TspRI | CASTG | 8 cut(s) | 70, 420, 472, 752, 834, 1474, 1533, 1953 |
| Tth111I | GACNNNGTC | 1 cut(s) | 74 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 467 |
| VpaK11BI | GGWCC | 4 cut(s) | 306, 1041, 1473, 1809 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 407, 572, 1146 |
| XapI | RAATTY | 7 cut(s) | 163, 451, 504, 553, 628, 1047, 1793 |
| XceI | RCATGY | 2 cut(s) | 890, 1013 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 131, 1610 |
| XmiI | GTMKAC | 1 cut(s) | 69 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1740 |
| XspI | CTAG | 2 cut(s) | 129, 1400 |
| ZrmI | AGTACT | 1 cut(s) | 949 |