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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 228 |
| Acc36I | ACCTGC | 1 cut(s) | 1418 |
| AccB1I | GGYRCC | 1 cut(s) | 769 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 686 |
| AccI | GTMKAC | 2 cut(s) | 789, 921 |
| AcoI | YGGCCR | 2 cut(s) | 3, 178 |
| AcsI | RAATTY | 1 cut(s) | 14 |
| AcyI | GRCGYC | 1 cut(s) | 770 |
| AdeI | CACNNNGTG | 1 cut(s) | 524 |
| AfaI | GTAC | 3 cut(s) | 221, 878, 1100 |
| AjnI | CCWGG | 2 cut(s) | 611, 1093 |
| AluBI | AGCT | 5 cut(s) | 51, 629, 762, 992, 1037 |
| AluI | AGCT | 5 cut(s) | 51, 629, 762, 992, 1037 |
| Alw21I | GWGCWC | 6 cut(s) | 100, 817, 949, 994, 1039, 1141 |
| Alw26I | GTCTC | 3 cut(s) | 117, 1240, 1283 |
| Alw44I | GTGCAC | 1 cut(s) | 945 |
| Ama87I | CYCGRG | 2 cut(s) | 1416, 1469 |
| AoxI | GGCC | 4 cut(s) | 3, 178, 1185, 1450 |
| ApaI | GGGCCC | 1 cut(s) | 1454 |
| ApaLI | GTGCAC | 1 cut(s) | 945 |
| ApeKI | GCWGC | 4 cut(s) | 1325, 1406, 1409, 1412 |
| ApoI | RAATTY | 1 cut(s) | 14 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1338, 1370 |
| AspLEI | GCGC | 5 cut(s) | 229, 772, 860, 902, 1087 |
| AspS9I | GGNCC | 3 cut(s) | 1186, 1450, 1451 |
| AsuC2I | CCSGG | 1 cut(s) | 1197 |
| AvaI | CYCGRG | 2 cut(s) | 1416, 1469 |
| BaeGI | GKGCMC | 2 cut(s) | 949, 1454 |
| BalI | TGGCCA | 2 cut(s) | 5, 180 |
| BanI | GGYRCC | 1 cut(s) | 769 |
| BanII | GRGCYC | 3 cut(s) | 994, 1039, 1454 |
| Bbv12I | GWGCWC | 6 cut(s) | 100, 817, 949, 994, 1039, 1141 |
| BbvI | GCAGC | 4 cut(s) | 1337, 1393, 1396, 1424 |
| BceAI | ACGGC | 4 cut(s) | 682, 1211, 1313, 1402 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1385, 1419 |
| BciT130I | CCWGG | 2 cut(s) | 613, 1095 |
| BcnI | CCSGG | 1 cut(s) | 1197 |
| BcoDI | GTCTC | 3 cut(s) | 117, 1240, 1283 |
| BfaI | CTAG | 1 cut(s) | 1241 |
| BfmI | CTRYAG | 2 cut(s) | 36, 1410 |
| BfoI | RGCGCY | 1 cut(s) | 773 |
| BfuAI | ACCTGC | 1 cut(s) | 1418 |
| BisI | GCNGC | 6 cut(s) | 1326, 1390, 1393, 1407, 1410, 1413 |
| BlsI | GCNGC | 6 cut(s) | 1327, 1391, 1394, 1408, 1411, 1414 |
| Bme1390I | CCNGG | 3 cut(s) | 613, 1095, 1197 |
| BmeT110I | CYCGRG | 2 cut(s) | 1416, 1469 |
| BmgT120I | GGNCC | 3 cut(s) | 1186, 1450, 1451 |
| BmiI | GGNNCC | 6 cut(s) | 675, 771, 1188, 1320, 1451, 1452 |
| BmrFI | CCNGG | 3 cut(s) | 613, 1095, 1197 |
| BmrI | ACTGGG | 4 cut(s) | 516, 798, 930, 975 |
| BmsI | GCATC | 4 cut(s) | 152, 451, 584, 1170 |
| BmuI | ACTGGG | 4 cut(s) | 516, 798, 930, 975 |
| BpmI | CTGGAG | 2 cut(s) | 609, 1077 |
| Bpu10I | CCTNAGC | 2 cut(s) | 339, 507 |
| BpuMI | CCSGG | 1 cut(s) | 1197 |
| BsaHI | GRCGYC | 1 cut(s) | 770 |
| BsaI | GGTCTC | 1 cut(s) | 1283 |
| BsaJI | CCNNGG | 5 cut(s) | 6, 175, 612, 1094, 1417 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 72, 102 |
| Bse1I | ACTGG | 6 cut(s) | 522, 804, 830, 936, 981, 1188 |
| BseBI | CCWGG | 2 cut(s) | 613, 1095 |
| BseDI | CCNNGG | 5 cut(s) | 6, 175, 612, 1094, 1417 |
| BseGI | GGATG | 3 cut(s) | 196, 1161, 1507 |
| BseNI | ACTGG | 6 cut(s) | 522, 804, 830, 936, 981, 1188 |
| BseSI | GKGCMC | 2 cut(s) | 949, 1454 |
| BseXI | GCAGC | 4 cut(s) | 1337, 1393, 1396, 1424 |
| BseYI | CCCAGC | 1 cut(s) | 758 |
| BshFI | GGCC | 4 cut(s) | 5, 180, 1187, 1452 |
| BshNI | GGYRCC | 1 cut(s) | 769 |
| BsiHKAI | GWGCWC | 6 cut(s) | 100, 817, 949, 994, 1039, 1141 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1416, 1469 |
| BsiSI | CCGG | 1 cut(s) | 1197 |
| BsmAI | GTCTC | 3 cut(s) | 117, 1240, 1283 |
| BsmBI | CGTCTC | 1 cut(s) | 117 |
| BsmI | GAATGC | 2 cut(s) | 1250, 1262 |
| BsnI | GGCC | 4 cut(s) | 5, 180, 1187, 1452 |
| Bso31I | GGTCTC | 1 cut(s) | 1283 |
| BsoBI | CYCGRG | 2 cut(s) | 1416, 1469 |
| Bsp120I | GGGCCC | 1 cut(s) | 1450 |
| Bsp1286I | GDGCHC | 7 cut(s) | 100, 817, 949, 994, 1039, 1141, 1454 |
| Bsp19I | CCATGG | 1 cut(s) | 175 |
| BspANI | GGCC | 4 cut(s) | 5, 180, 1187, 1452 |
| BspHI | TCATGA | 1 cut(s) | 1462 |
| BspLI | GGNNCC | 6 cut(s) | 675, 771, 1188, 1320, 1451, 1452 |
| BspMAI | CTGCAG | 1 cut(s) | 1414 |
| BspMI | ACCTGC | 1 cut(s) | 1418 |
| BspT107I | GGYRCC | 1 cut(s) | 769 |
| BspTNI | GGTCTC | 1 cut(s) | 1283 |
| BsrI | ACTGG | 6 cut(s) | 522, 804, 830, 936, 981, 1188 |
| BssECI | CCNNGG | 5 cut(s) | 6, 175, 612, 1094, 1417 |
| BssNAI | GTATAC | 2 cut(s) | 790, 922 |
| BssNI | GRCGYC | 1 cut(s) | 770 |
| BssT1I | CCWWGG | 2 cut(s) | 6, 175 |
| Bst1107I | GTATAC | 2 cut(s) | 790, 922 |
| Bst2UI | CCWGG | 2 cut(s) | 613, 1095 |
| Bst4CI | ACNGT | 6 cut(s) | 111, 219, 1136, 1170, 1219, 1366 |
| Bst6I | CTCTTC | 1 cut(s) | 1301 |
| BstACI | GRCGYC | 1 cut(s) | 770 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1406 |
| BstDEI | CTNAG | 2 cut(s) | 339, 507 |
| BstDSI | CCRYGG | 1 cut(s) | 175 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1155 |
| BstF5I | GGATG | 3 cut(s) | 196, 1161, 1507 |
| BstH2I | RGCGCY | 1 cut(s) | 773 |
| BstHHI | GCGC | 5 cut(s) | 229, 772, 860, 902, 1087 |
| BstMAI | GTCTC | 3 cut(s) | 117, 1240, 1283 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1406, 1412, 1490 |
| BstNI | CCWGG | 2 cut(s) | 613, 1095 |
| BstSCI | CCNGG | 3 cut(s) | 611, 1093, 1195 |
| BstSFI | CTRYAG | 2 cut(s) | 36, 1410 |
| BstSLI | GKGCMC | 2 cut(s) | 949, 1454 |
| BstV1I | GCAGC | 4 cut(s) | 1337, 1393, 1396, 1424 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1183 |
| BstZ17I | GTATAC | 2 cut(s) | 790, 922 |
| BsuRI | GGCC | 4 cut(s) | 5, 180, 1187, 1452 |
| BtgI | CCRYGG | 1 cut(s) | 175 |
| BtgZI | GCGATG | 2 cut(s) | 155, 1207 |
| BtsCI | GGATG | 3 cut(s) | 196, 1161, 1507 |
| BtsI | GCAGTG | 1 cut(s) | 650 |
| BtsIMutI | CAGTG | 5 cut(s) | 529, 650, 811, 943, 1215 |
| BveI | ACCTGC | 1 cut(s) | 1418 |
| CciI | TCATGA | 1 cut(s) | 1462 |
| CfoI | GCGC | 5 cut(s) | 229, 772, 860, 902, 1087 |
| Cfr13I | GGNCC | 3 cut(s) | 1186, 1450, 1451 |
| Csp6I | GTAC | 3 cut(s) | 220, 877, 1099 |
| CviQI | GTAC | 3 cut(s) | 220, 877, 1099 |
| DdeI | CTNAG | 2 cut(s) | 339, 507 |
| DinI | GGCGCC | 1 cut(s) | 771 |
| DraIII | CACNNNGTG | 1 cut(s) | 524 |
| EaeI | YGGCCR | 2 cut(s) | 3, 178 |
| Eam1104I | CTCTTC | 1 cut(s) | 1301 |
| EarI | CTCTTC | 1 cut(s) | 1301 |
| EciI | GGCGGA | 3 cut(s) | 144, 423, 456 |
| Ecl136II | GAGCTC | 2 cut(s) | 992, 1037 |
| Eco130I | CCWWGG | 2 cut(s) | 6, 175 |
| Eco24I | GRGCYC | 3 cut(s) | 994, 1039, 1454 |
| Eco31I | GGTCTC | 1 cut(s) | 1283 |
| Eco53kI | GAGCTC | 2 cut(s) | 992, 1037 |
| Eco88I | CYCGRG | 2 cut(s) | 1416, 1469 |
| EcoICRI | GAGCTC | 2 cut(s) | 992, 1037 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1155 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1450 |
| EcoRII | CCWGG | 2 cut(s) | 611, 1093 |
| EcoT14I | CCWWGG | 2 cut(s) | 6, 175 |
| EcoT38I | GRGCYC | 3 cut(s) | 994, 1039, 1454 |
| EgeI | GGCGCC | 1 cut(s) | 771 |
| EheI | GGCGCC | 1 cut(s) | 771 |
| ErhI | CCWWGG | 2 cut(s) | 6, 175 |
| Esp3I | CGTCTC | 1 cut(s) | 117 |
| FauI | CCCGC | 2 cut(s) | 1193, 1330 |
| FblI | GTMKAC | 2 cut(s) | 789, 921 |
| Fnu4HI | GCNGC | 6 cut(s) | 1326, 1390, 1393, 1407, 1410, 1413 |
| FokI | GGATG | 3 cut(s) | 183, 1148, 1494 |
| FriOI | GRGCYC | 3 cut(s) | 994, 1039, 1454 |
| Fsp4HI | GCNGC | 6 cut(s) | 1326, 1390, 1393, 1407, 1410, 1413 |
| FspBI | CTAG | 1 cut(s) | 1241 |
| FspI | TGCGCA | 1 cut(s) | 228 |
| GlaI | GCGC | 5 cut(s) | 228, 771, 859, 901, 1086 |
| GluI | GCNGC | 6 cut(s) | 1326, 1390, 1393, 1407, 1410, 1413 |
| GsaI | CCCAGC | 1 cut(s) | 762 |
| GsuI | CTGGAG | 2 cut(s) | 609, 1077 |
| HaeII | RGCGCY | 1 cut(s) | 773 |
| HaeIII | GGCC | 4 cut(s) | 5, 180, 1187, 1452 |
| HapII | CCGG | 1 cut(s) | 1197 |
| HhaI | GCGC | 5 cut(s) | 229, 772, 860, 902, 1087 |
| Hin1I | GRCGYC | 1 cut(s) | 770 |
| Hin6I | GCGC | 5 cut(s) | 227, 770, 858, 900, 1085 |
| HinP1I | GCGC | 5 cut(s) | 227, 770, 858, 900, 1085 |
| HpaII | CCGG | 1 cut(s) | 1197 |
| Hpy166II | GTNNAC | 5 cut(s) | 790, 922, 947, 967, 1384 |
| Hpy188I | TCNGA | 1 cut(s) | 1157 |
| Hpy188III | TCNNGA | 1 cut(s) | 1463 |
| Hpy8I | GTNNAC | 5 cut(s) | 790, 922, 947, 967, 1384 |
| HpyAV | CCTTC | 7 cut(s) | 87, 483, 489, 803, 935, 1277, 1436 |
| HpyCH4III | ACNGT | 6 cut(s) | 111, 219, 1136, 1170, 1219, 1366 |
| HpyCH4V | TGCA | 4 cut(s) | 575, 947, 1250, 1412 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1406, 1412, 1490 |
| HpyF3I | CTNAG | 2 cut(s) | 339, 507 |
| Hsp92I | GRCGYC | 1 cut(s) | 770 |
| HspAI | GCGC | 5 cut(s) | 227, 770, 858, 900, 1085 |
| KasI | GGCGCC | 1 cut(s) | 769 |
| LmnI | GCTCC | 9 cut(s) | 103, 679, 820, 989, 997, 1034, 1042, 1144, 1424 |
| Lsp1109I | GCAGC | 4 cut(s) | 1337, 1393, 1396, 1424 |
| LweI | GCATC | 4 cut(s) | 152, 451, 584, 1170 |
| MaeI | CTAG | 1 cut(s) | 1241 |
| MaeIII | GTNAC | 3 cut(s) | 128, 136, 1430 |
| MboII | GAAGA | 8 cut(s) | 999, 1246, 1309, 1312, 1315, 1318, 1358, 1361 |
| MhlI | GDGCHC | 7 cut(s) | 100, 817, 949, 994, 1039, 1141, 1454 |
| MlsI | TGGCCA | 2 cut(s) | 5, 180 |
| MluCI | AATT | 1 cut(s) | 14 |
| MluNI | TGGCCA | 2 cut(s) | 5, 180 |
| Mly113I | GGCGCC | 1 cut(s) | 770 |
| Mox20I | TGGCCA | 2 cut(s) | 5, 180 |
| MscI | TGGCCA | 2 cut(s) | 5, 180 |
| MseI | TTAA | 1 cut(s) | 1519 |
| MslI | CAYNNNNRTG | 8 cut(s) | 174, 273, 327, 447, 495, 543, 591, 1226 |
| Msp20I | TGGCCA | 2 cut(s) | 5, 180 |
| MspI | CCGG | 1 cut(s) | 1197 |
| MspR9I | CCNGG | 3 cut(s) | 613, 1095, 1197 |
| Mva1269I | GAATGC | 2 cut(s) | 1250, 1262 |
| MvaI | CCWGG | 2 cut(s) | 613, 1095 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1406, 1412, 1490 |
| NarI | GGCGCC | 1 cut(s) | 770 |
| NciI | CCSGG | 1 cut(s) | 1197 |
| NcoI | CCATGG | 1 cut(s) | 175 |
| NlaIV | GGNNCC | 6 cut(s) | 675, 771, 1188, 1320, 1451, 1452 |
| NmeAIII | GCCGAG | 1 cut(s) | 650 |
| NsbI | TGCGCA | 1 cut(s) | 228 |
| PagI | TCATGA | 1 cut(s) | 1462 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1392 |
| PctI | GAATGC | 2 cut(s) | 1250, 1262 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 686 |
| PkrI | GCNGC | 6 cut(s) | 1327, 1391, 1394, 1408, 1411, 1414 |
| PluTI | GGCGCC | 1 cut(s) | 773 |
| Psp124BI | GAGCTC | 2 cut(s) | 994, 1039 |
| Psp6I | CCWGG | 2 cut(s) | 611, 1093 |
| PspFI | CCCAGC | 1 cut(s) | 758 |
| PspGI | CCWGG | 2 cut(s) | 611, 1093 |
| PspN4I | GGNNCC | 6 cut(s) | 675, 771, 1188, 1320, 1451, 1452 |
| PspOMI | GGGCCC | 1 cut(s) | 1450 |
| PspPI | GGNCC | 3 cut(s) | 1186, 1450, 1451 |
| PstI | CTGCAG | 1 cut(s) | 1414 |
| RsaI | GTAC | 3 cut(s) | 221, 878, 1100 |
| RsaNI | GTAC | 3 cut(s) | 220, 877, 1099 |
| RseI | CAYNNNNRTG | 8 cut(s) | 174, 273, 327, 447, 495, 543, 591, 1226 |
| SacI | GAGCTC | 2 cut(s) | 994, 1039 |
| SaqAI | TTAA | 1 cut(s) | 1519 |
| SatI | GCNGC | 6 cut(s) | 1326, 1390, 1393, 1407, 1410, 1413 |
| Sau96I | GGNCC | 3 cut(s) | 1186, 1450, 1451 |
| ScrFI | CCNGG | 3 cut(s) | 613, 1095, 1197 |
| SduI | GDGCHC | 7 cut(s) | 100, 817, 949, 994, 1039, 1141, 1454 |
| SfaNI | GCATC | 4 cut(s) | 152, 451, 584, 1170 |
| SfcI | CTRYAG | 2 cut(s) | 36, 1410 |
| SfoI | GGCGCC | 1 cut(s) | 771 |
| SmiMI | CAYNNNNRTG | 8 cut(s) | 174, 273, 327, 447, 495, 543, 591, 1226 |
| Sse9I | AATT | 1 cut(s) | 14 |
| SspDI | GGCGCC | 1 cut(s) | 769 |
| SspMI | CTAG | 1 cut(s) | 1241 |
| SstI | GAGCTC | 2 cut(s) | 994, 1039 |
| StyD4I | CCNGG | 3 cut(s) | 611, 1093, 1195 |
| StyI | CCWWGG | 2 cut(s) | 6, 175 |
| TaaI | ACNGT | 6 cut(s) | 111, 219, 1136, 1170, 1219, 1366 |
| TaqII | GACCGA | 1 cut(s) | 1265 |
| TasI | AATT | 1 cut(s) | 14 |
| TatI | WGTACW | 1 cut(s) | 219 |
| TauI | GCSGC | 2 cut(s) | 1392, 1395 |
| Tru1I | TTAA | 1 cut(s) | 1519 |
| Tru9I | TTAA | 1 cut(s) | 1519 |
| TscAI | CASTG | 5 cut(s) | 529, 657, 811, 943, 1222 |
| TseI | GCWGC | 4 cut(s) | 1325, 1406, 1409, 1412 |
| TspDTI | ATGAA | 4 cut(s) | 999, 1194, 1374, 1479 |
| TspGWI | ACGGA | 3 cut(s) | 775, 907, 952 |
| TspRI | CASTG | 5 cut(s) | 529, 657, 811, 943, 1222 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 686 |
| VneI | GTGCAC | 1 cut(s) | 945 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1155 |
| XapI | RAATTY | 1 cut(s) | 14 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 609 |
| XmiI | GTMKAC | 2 cut(s) | 789, 921 |
| XspI | CTAG | 1 cut(s) | 1241 |