Domain of unknown function (DUF3475)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1483 |
| Acc65I | GGTACC | 1 cut(s) | 232 |
| AccB1I | GGYRCC | 2 cut(s) | 211, 232 |
| AccI | GTMKAC | 1 cut(s) | 842 |
| AciI | CCGC | 1 cut(s) | 380 |
| AclWI | GGATC | 7 cut(s) | 54, 716, 750, 1141, 1856, 1869, 1941 |
| AcsI | RAATTY | 6 cut(s) | 433, 486, 535, 610, 1000, 1775 |
| AcuI | CTGAAG | 1 cut(s) | 618 |
| AfaI | GTAC | 4 cut(s) | 234, 376, 606, 1808 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 54, 208, 449, 556, 599, 1130, 1878 |
| AjnI | CCWGG | 2 cut(s) | 1276, 1793 |
| Alw21I | GWGCWC | 1 cut(s) | 356 |
| Alw26I | GTCTC | 6 cut(s) | 192, 212, 324, 1224, 1499, 1842 |
| AlwI | GGATC | 7 cut(s) | 54, 716, 750, 1141, 1856, 1869, 1941 |
| AoxI | GGCC | 1 cut(s) | 828 |
| ApeKI | GCWGC | 5 cut(s) | 80, 650, 653, 1270, 1394 |
| ApoI | RAATTY | 6 cut(s) | 433, 486, 535, 610, 1000, 1775 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1295, 1327, 1439, 1471 |
| Asp718I | GGTACC | 1 cut(s) | 232 |
| AspLEI | GCGC | 1 cut(s) | 1299 |
| AsuC2I | CCSGG | 1 cut(s) | 1755 |
| AsuHPI | GGTGA | 3 cut(s) | 241, 478, 956 |
| AsuII | TTCGAA | 1 cut(s) | 1960 |
| BaeGI | GKGCMC | 1 cut(s) | 153 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 216, 249, 1200, 1233 |
| BamHI | GGATCC | 1 cut(s) | 1861 |
| BanI | GGYRCC | 2 cut(s) | 211, 232 |
| BanII | GRGCYC | 2 cut(s) | 246, 356 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1551, 1583 |
| BauI | CACGAG | 1 cut(s) | 1248 |
| Bbv12I | GWGCWC | 1 cut(s) | 356 |
| BbvCI | CCTCAGC | 1 cut(s) | 1496 |
| BbvI | GCAGC | 5 cut(s) | 67, 637, 665, 1257, 1381 |
| BccI | CCATC | 6 cut(s) | 170, 175, 601, 820, 1152, 1696 |
| BceAI | ACGGC | 1 cut(s) | 283 |
| BciT130I | CCWGG | 2 cut(s) | 1278, 1795 |
| BcnI | CCSGG | 1 cut(s) | 1755 |
| BcoDI | GTCTC | 6 cut(s) | 192, 212, 324, 1224, 1499, 1842 |
| BfaI | CTAG | 3 cut(s) | 285, 444, 1026 |
| BfmI | CTRYAG | 2 cut(s) | 651, 1307 |
| BfuAI | ACCTGC | 1 cut(s) | 1483 |
| BglII | AGATCT | 2 cut(s) | 1086, 1613 |
| BisI | GCNGC | 5 cut(s) | 81, 651, 654, 1271, 1395 |
| BlsI | GCNGC | 5 cut(s) | 82, 652, 655, 1272, 1396 |
| Bme1390I | CCNGG | 3 cut(s) | 1278, 1755, 1795 |
| BmiI | GGNNCC | 3 cut(s) | 213, 234, 1863 |
| BmrFI | CCNGG | 3 cut(s) | 1278, 1755, 1795 |
| BmrI | ACTGGG | 1 cut(s) | 1461 |
| BmsI | GCATC | 2 cut(s) | 790, 804 |
| BmuI | ACTGGG | 1 cut(s) | 1461 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 908, 940 |
| BpmI | CTGGAG | 1 cut(s) | 165 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1496 |
| Bpu14I | TTCGAA | 1 cut(s) | 1960 |
| BpuEI | CTTGAG | 1 cut(s) | 1571 |
| BpuMI | CCSGG | 1 cut(s) | 1755 |
| BsaI | GGTCTC | 2 cut(s) | 192, 212 |
| BsaJI | CCNNGG | 2 cut(s) | 415, 1754 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 138, 168, 681, 711 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 54, 208, 449, 556, 599, 1130, 1878 |
| Bse1I | ACTGG | 4 cut(s) | 167, 838, 1456, 1834 |
| Bse3DI | GCAATG | 2 cut(s) | 1104, 1389 |
| BseBI | CCWGG | 2 cut(s) | 1278, 1795 |
| BseDI | CCNNGG | 2 cut(s) | 415, 1754 |
| BseGI | GGATG | 5 cut(s) | 79, 186, 637, 805, 1688 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 54, 208, 449, 556, 599, 1130, 1878 |
| BseMI | GCAATG | 2 cut(s) | 1104, 1389 |
| BseMII | CTCAG | 7 cut(s) | 335, 740, 960, 1151, 1449, 1487, 1527 |
| BseNI | ACTGG | 4 cut(s) | 167, 838, 1456, 1834 |
| BseRI | GAGGAG | 2 cut(s) | 70, 929 |
| BseSI | GKGCMC | 1 cut(s) | 153 |
| BseXI | GCAGC | 5 cut(s) | 67, 637, 665, 1257, 1381 |
| BseYI | CCCAGC | 1 cut(s) | 1679 |
| BshFI | GGCC | 1 cut(s) | 830 |
| BshNI | GGYRCC | 2 cut(s) | 211, 232 |
| BsiHKAI | GWGCWC | 1 cut(s) | 356 |
| BsiSI | CCGG | 1 cut(s) | 1754 |
| BslFI | GGGAC | 3 cut(s) | 396, 1325, 1612 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 54, 208, 449, 556, 599, 1130, 1878 |
| BsmAI | GTCTC | 6 cut(s) | 192, 212, 324, 1224, 1499, 1842 |
| BsmFI | GGGAC | 3 cut(s) | 396, 1325, 1612 |
| BsmI | GAATGC | 1 cut(s) | 270 |
| BsnI | GGCC | 1 cut(s) | 830 |
| Bso31I | GGTCTC | 2 cut(s) | 192, 212 |
| Bsp119I | TTCGAA | 1 cut(s) | 1960 |
| Bsp1286I | GDGCHC | 3 cut(s) | 153, 246, 356 |
| BspACI | CCGC | 1 cut(s) | 380 |
| BspANI | GGCC | 1 cut(s) | 830 |
| BspCNI | CTCAG | 7 cut(s) | 334, 739, 961, 1150, 1450, 1488, 1528 |
| BspHI | TCATGA | 1 cut(s) | 859 |
| BspLI | GGNNCC | 3 cut(s) | 213, 234, 1863 |
| BspMAI | CTGCAG | 1 cut(s) | 655 |
| BspMI | ACCTGC | 1 cut(s) | 1483 |
| BspPI | GGATC | 7 cut(s) | 54, 716, 750, 1141, 1856, 1869, 1941 |
| BspQI | GCTCTTC | 1 cut(s) | 1323 |
| BspT104I | TTCGAA | 1 cut(s) | 1960 |
| BspT107I | GGYRCC | 2 cut(s) | 211, 232 |
| BspTNI | GGTCTC | 2 cut(s) | 192, 212 |
| BsrDI | GCAATG | 2 cut(s) | 1104, 1389 |
| BsrI | ACTGG | 4 cut(s) | 167, 838, 1456, 1834 |
| BssECI | CCNNGG | 2 cut(s) | 415, 1754 |
| BssNAI | GTATAC | 1 cut(s) | 843 |
| BssSI | CACGAG | 1 cut(s) | 1248 |
| BssT1I | CCWWGG | 1 cut(s) | 415 |
| Bst1107I | GTATAC | 1 cut(s) | 843 |
| Bst2BI | CACGAG | 1 cut(s) | 1248 |
| Bst2UI | CCWGG | 2 cut(s) | 1278, 1795 |
| Bst4CI | ACNGT | 5 cut(s) | 67, 100, 1311, 1339, 1705 |
| Bst6I | CTCTTC | 2 cut(s) | 1032, 1323 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 89 |
| BstBI | TTCGAA | 1 cut(s) | 1960 |
| BstC8I | GCNNGC | 2 cut(s) | 327, 870 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 554, 1128 |
| BstF5I | GGATG | 5 cut(s) | 79, 186, 637, 805, 1688 |
| BstHHI | GCGC | 1 cut(s) | 1299 |
| BstMAI | GTCTC | 6 cut(s) | 192, 212, 324, 1224, 1499, 1842 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 89, 134, 377, 1172 |
| BstNI | CCWGG | 2 cut(s) | 1278, 1795 |
| BstNSI | RCATGY | 2 cut(s) | 128, 995 |
| BstSCI | CCNGG | 3 cut(s) | 1276, 1753, 1793 |
| BstSFI | CTRYAG | 2 cut(s) | 651, 1307 |
| BstSLI | GKGCMC | 1 cut(s) | 153 |
| BstV1I | GCAGC | 5 cut(s) | 67, 637, 665, 1257, 1381 |
| BstX2I | RGATCY | 7 cut(s) | 59, 721, 1086, 1133, 1613, 1861, 1933 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 229 |
| BstYI | RGATCY | 7 cut(s) | 59, 721, 1086, 1133, 1613, 1861, 1933 |
| BstZ17I | GTATAC | 1 cut(s) | 843 |
| BsuRI | GGCC | 1 cut(s) | 830 |
| BtsCI | GGATG | 5 cut(s) | 79, 186, 637, 805, 1688 |
| BtsI | GCAGTG | 2 cut(s) | 1382, 1782 |
| BtsIMutI | CAGTG | 6 cut(s) | 63, 96, 734, 1382, 1449, 1782 |
| BveI | ACCTGC | 1 cut(s) | 1483 |
| Cac8I | GCNNGC | 2 cut(s) | 327, 870 |
| CciI | TCATGA | 1 cut(s) | 859 |
| CfoI | GCGC | 1 cut(s) | 1299 |
| CseI | GACGC | 1 cut(s) | 150 |
| CsiI | ACCWGGT | 2 cut(s) | 1276, 1793 |
| Csp6I | GTAC | 4 cut(s) | 233, 375, 605, 1807 |
| CviAII | CATG | 7 cut(s) | 125, 427, 860, 992, 1045, 1418, 1517 |
| CviQI | GTAC | 4 cut(s) | 233, 375, 605, 1807 |
| DraI | TTTAAA | 1 cut(s) | 438 |
| Eam1104I | CTCTTC | 2 cut(s) | 1032, 1323 |
| EarI | CTCTTC | 2 cut(s) | 1032, 1323 |
| Ecl136II | GAGCTC | 1 cut(s) | 354 |
| Eco130I | CCWWGG | 1 cut(s) | 415 |
| Eco24I | GRGCYC | 2 cut(s) | 246, 356 |
| Eco31I | GGTCTC | 2 cut(s) | 192, 212 |
| Eco32I | GATATC | 1 cut(s) | 1912 |
| Eco53kI | GAGCTC | 1 cut(s) | 354 |
| Eco57I | CTGAAG | 1 cut(s) | 618 |
| EcoICRI | GAGCTC | 1 cut(s) | 354 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 554, 1128 |
| EcoRII | CCWGG | 2 cut(s) | 1276, 1793 |
| EcoRV | GATATC | 1 cut(s) | 1912 |
| EcoT14I | CCWWGG | 1 cut(s) | 415 |
| EcoT38I | GRGCYC | 2 cut(s) | 246, 356 |
| ErhI | CCWWGG | 1 cut(s) | 415 |
| FaeI | CATG | 7 cut(s) | 128, 430, 863, 995, 1048, 1421, 1520 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1707, 1739 |
| FaqI | GGGAC | 3 cut(s) | 396, 1325, 1612 |
| FatI | CATG | 7 cut(s) | 124, 426, 859, 991, 1044, 1417, 1516 |
| FauI | CCCGC | 1 cut(s) | 373 |
| FblI | GTMKAC | 1 cut(s) | 842 |
| Fnu4HI | GCNGC | 5 cut(s) | 81, 651, 654, 1271, 1395 |
| FokI | GGATG | 5 cut(s) | 86, 193, 644, 812, 1675 |
| FriOI | GRGCYC | 2 cut(s) | 246, 356 |
| Fsp4HI | GCNGC | 5 cut(s) | 81, 651, 654, 1271, 1395 |
| FspBI | CTAG | 3 cut(s) | 285, 444, 1026 |
| GlaI | GCGC | 1 cut(s) | 1298 |
| GluI | GCNGC | 5 cut(s) | 81, 651, 654, 1271, 1395 |
| GsaI | CCCAGC | 1 cut(s) | 1683 |
| GsuI | CTGGAG | 1 cut(s) | 165 |
| HaeIII | GGCC | 1 cut(s) | 830 |
| HapII | CCGG | 1 cut(s) | 1754 |
| HgaI | GACGC | 1 cut(s) | 150 |
| HhaI | GCGC | 1 cut(s) | 1299 |
| Hin1II | CATG | 7 cut(s) | 128, 430, 863, 995, 1048, 1421, 1520 |
| Hin6I | GCGC | 1 cut(s) | 1297 |
| HinP1I | GCGC | 1 cut(s) | 1297 |
| HincII | GTYRAC | 1 cut(s) | 1582 |
| HindII | GTYRAC | 1 cut(s) | 1582 |
| HindIII | AAGCTT | 1 cut(s) | 1052 |
| HinfI | GANTC | 3 cut(s) | 1030, 1538, 1608 |
| HpaII | CCGG | 1 cut(s) | 1754 |
| HphI | GGTGA | 3 cut(s) | 241, 478, 956 |
| Hpy166II | GTNNAC | 5 cut(s) | 843, 905, 1060, 1582, 1951 |
| Hpy188III | TCNNGA | 9 cut(s) | 285, 621, 687, 860, 911, 1034, 1550, 1715, 1772 |
| Hpy8I | GTNNAC | 5 cut(s) | 843, 905, 1060, 1582, 1951 |
| Hpy99I | CGWCG | 1 cut(s) | 166 |
| HpyAV | CCTTC | 6 cut(s) | 116, 368, 385, 593, 787, 1852 |
| HpyCH4III | ACNGT | 5 cut(s) | 67, 100, 1311, 1339, 1705 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 89, 134, 377, 1172 |
| Hsp92II | CATG | 7 cut(s) | 128, 430, 863, 995, 1048, 1421, 1520 |
| HspAI | GCGC | 1 cut(s) | 1297 |
| KpnI | GGTACC | 1 cut(s) | 236 |
| LguI | GCTCTTC | 1 cut(s) | 1323 |
| LmnI | GCTCC | 4 cut(s) | 50, 359, 1643, 1920 |
| Lsp1109I | GCAGC | 5 cut(s) | 67, 637, 665, 1257, 1381 |
| LweI | GCATC | 2 cut(s) | 790, 804 |
| MabI | ACCWGGT | 2 cut(s) | 1276, 1793 |
| MaeI | CTAG | 3 cut(s) | 285, 444, 1026 |
| MaeIII | GTNAC | 2 cut(s) | 466, 944 |
| MboII | GAAGA | 5 cut(s) | 680, 1018, 1049, 1340, 1721 |
| MfeI | CAATTG | 3 cut(s) | 519, 788, 821 |
| MflI | RGATCY | 7 cut(s) | 59, 721, 1086, 1133, 1613, 1861, 1933 |
| MhlI | GDGCHC | 3 cut(s) | 153, 246, 356 |
| MlyI | GAGTC | 1 cut(s) | 1547 |
| MmeI | TCCRAC | 3 cut(s) | 1414, 1576, 1865 |
| MslI | CAYNNNNRTG | 1 cut(s) | 29 |
| MspA1I | CMGCKG | 3 cut(s) | 656, 848, 1683 |
| MspI | CCGG | 1 cut(s) | 1754 |
| MspR9I | CCNGG | 3 cut(s) | 1278, 1755, 1795 |
| MunI | CAATTG | 3 cut(s) | 519, 788, 821 |
| Mva1269I | GAATGC | 1 cut(s) | 270 |
| MvaI | CCWGG | 2 cut(s) | 1278, 1795 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 89, 134, 377, 1172 |
| NciI | CCSGG | 1 cut(s) | 1755 |
| NlaIII | CATG | 7 cut(s) | 128, 430, 863, 995, 1048, 1421, 1520 |
| NlaIV | GGNNCC | 3 cut(s) | 213, 234, 1863 |
| NmuCI | GTSAC | 2 cut(s) | 466, 944 |
| NspI | RCATGY | 2 cut(s) | 128, 995 |
| NspV | TTCGAA | 1 cut(s) | 1960 |
| PagI | TCATGA | 1 cut(s) | 859 |
| PciSI | GCTCTTC | 1 cut(s) | 1323 |
| PctI | GAATGC | 1 cut(s) | 270 |
| PfeI | GAWTC | 2 cut(s) | 1030, 1608 |
| PkrI | GCNGC | 5 cut(s) | 82, 652, 655, 1272, 1396 |
| PleI | GAGTC | 1 cut(s) | 1546 |
| PpsI | GAGTC | 1 cut(s) | 1546 |
| Psp124BI | GAGCTC | 1 cut(s) | 356 |
| Psp6I | CCWGG | 2 cut(s) | 1276, 1793 |
| PspFI | CCCAGC | 1 cut(s) | 1679 |
| PspGI | CCWGG | 2 cut(s) | 1276, 1793 |
| PspN4I | GGNNCC | 3 cut(s) | 213, 234, 1863 |
| PstI | CTGCAG | 1 cut(s) | 655 |
| PsuI | RGATCY | 7 cut(s) | 59, 721, 1086, 1133, 1613, 1861, 1933 |
| PvuII | CAGCTG | 3 cut(s) | 656, 848, 1683 |
| RsaI | GTAC | 4 cut(s) | 234, 376, 606, 1808 |
| RsaNI | GTAC | 4 cut(s) | 233, 375, 605, 1807 |
| RseI | CAYNNNNRTG | 1 cut(s) | 29 |
| SacI | GAGCTC | 1 cut(s) | 356 |
| SapI | GCTCTTC | 1 cut(s) | 1323 |
| SatI | GCNGC | 5 cut(s) | 81, 651, 654, 1271, 1395 |
| SchI | GAGTC | 1 cut(s) | 1547 |
| ScrFI | CCNGG | 3 cut(s) | 1278, 1755, 1795 |
| SduI | GDGCHC | 3 cut(s) | 153, 246, 356 |
| SexAI | ACCWGGT | 2 cut(s) | 1276, 1793 |
| SfaNI | GCATC | 2 cut(s) | 790, 804 |
| SfcI | CTRYAG | 2 cut(s) | 651, 1307 |
| SfuI | TTCGAA | 1 cut(s) | 1960 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 29 |
| SmlI | CTYRAG | 1 cut(s) | 1550 |
| SmoI | CTYRAG | 1 cut(s) | 1550 |
| SsiI | CCGC | 1 cut(s) | 380 |
| SspI | AATATT | 1 cut(s) | 1192 |
| SspMI | CTAG | 3 cut(s) | 285, 444, 1026 |
| SstI | GAGCTC | 1 cut(s) | 356 |
| StyD4I | CCNGG | 3 cut(s) | 1276, 1753, 1793 |
| StyI | CCWWGG | 1 cut(s) | 415 |
| TaaI | ACNGT | 5 cut(s) | 67, 100, 1311, 1339, 1705 |
| TaqI | TCGA | 5 cut(s) | 53, 620, 1914, 1945, 1960 |
| TaqII | GACCGA | 2 cut(s) | 1212, 1502 |
| TfiI | GAWTC | 2 cut(s) | 1030, 1608 |
| TscAI | CASTG | 6 cut(s) | 70, 103, 734, 1382, 1456, 1789 |
| TseFI | GTSAC | 2 cut(s) | 466, 944 |
| TseI | GCWGC | 5 cut(s) | 80, 650, 653, 1270, 1394 |
| Tsp45I | GTSAC | 2 cut(s) | 466, 944 |
| TspDTI | ATGAA | 7 cut(s) | 13, 17, 648, 1107, 1478, 1559, 1649 |
| TspGWI | ACGGA | 1 cut(s) | 1879 |
| TspRI | CASTG | 6 cut(s) | 70, 103, 734, 1382, 1456, 1789 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 554, 1128 |
| XapI | RAATTY | 6 cut(s) | 433, 486, 535, 610, 1000, 1775 |
| XbaI | TCTAGA | 1 cut(s) | 284 |
| XceI | RCATGY | 2 cut(s) | 128, 995 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1592 |
| XmiI | GTMKAC | 1 cut(s) | 842 |
| XspI | CTAG | 3 cut(s) | 285, 444, 1026 |