Domain of unknown function (DUF3475)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 56 |
| AjnI | CCWGG | 1 cut(s) | 74 |
| AoxI | GGCC | 1 cut(s) | 161 |
| ApoI | RAATTY | 1 cut(s) | 56 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 176, 208 |
| AspS9I | GGNCC | 2 cut(s) | 72, 142 |
| AsuC2I | CCSGG | 1 cut(s) | 36 |
| AsuII | TTCGAA | 1 cut(s) | 241 |
| AvaII | GGWCC | 2 cut(s) | 72, 142 |
| BccI | CCATC | 1 cut(s) | 172 |
| BciT130I | CCWGG | 1 cut(s) | 76 |
| BcnI | CCSGG | 1 cut(s) | 36 |
| BlpI | GCTNAGC | 1 cut(s) | 98 |
| Bme1390I | CCNGG | 2 cut(s) | 36, 76 |
| Bme18I | GGWCC | 2 cut(s) | 72, 142 |
| BmgT120I | GGNCC | 2 cut(s) | 72, 142 |
| BmiI | GGNNCC | 1 cut(s) | 144 |
| BmrFI | CCNGG | 2 cut(s) | 36, 76 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 98 |
| Bpu14I | TTCGAA | 1 cut(s) | 241 |
| BpuEI | CTTGAG | 1 cut(s) | 52 |
| BpuMI | CCSGG | 1 cut(s) | 36 |
| Bsa29I | ATCGAT | 1 cut(s) | 222 |
| BsaJI | CCNNGG | 1 cut(s) | 35 |
| BsaWI | WCCGGW | 1 cut(s) | 198 |
| Bse118I | RCCGGY | 1 cut(s) | 93 |
| Bse1I | ACTGG | 1 cut(s) | 216 |
| BseBI | CCWGG | 1 cut(s) | 76 |
| BseCI | ATCGAT | 1 cut(s) | 222 |
| BseDI | CCNNGG | 1 cut(s) | 35 |
| BseGI | GGATG | 2 cut(s) | 164, 220 |
| BseMII | CTCAG | 1 cut(s) | 89 |
| BseNI | ACTGG | 1 cut(s) | 216 |
| Bsh1285I | CGRYCG | 2 cut(s) | 199, 226 |
| BshFI | GGCC | 1 cut(s) | 163 |
| BshVI | ATCGAT | 1 cut(s) | 222 |
| BsiEI | CGRYCG | 2 cut(s) | 199, 226 |
| BsiSI | CCGG | 3 cut(s) | 35, 94, 199 |
| BsnI | GGCC | 1 cut(s) | 163 |
| Bsp119I | TTCGAA | 1 cut(s) | 241 |
| Bsp143I | GATC | 2 cut(s) | 219, 223 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 98 |
| BspANI | GGCC | 1 cut(s) | 163 |
| BspCNI | CTCAG | 1 cut(s) | 90 |
| BspDI | ATCGAT | 1 cut(s) | 222 |
| BspLI | GGNNCC | 1 cut(s) | 144 |
| BspT104I | TTCGAA | 1 cut(s) | 241 |
| BsrFI | RCCGGY | 1 cut(s) | 93 |
| BsrI | ACTGG | 1 cut(s) | 216 |
| BssAI | RCCGGY | 1 cut(s) | 93 |
| BssECI | CCNNGG | 1 cut(s) | 35 |
| BssMI | GATC | 2 cut(s) | 219, 223 |
| Bst2UI | CCWGG | 1 cut(s) | 76 |
| BstBI | TTCGAA | 1 cut(s) | 241 |
| BstC8I | GCNNGC | 1 cut(s) | 86 |
| BstDEI | CTNAG | 1 cut(s) | 98 |
| BstF5I | GGATG | 2 cut(s) | 164, 220 |
| BstKTI | GATC | 2 cut(s) | 222, 226 |
| BstMBI | GATC | 2 cut(s) | 219, 223 |
| BstMCI | CGRYCG | 2 cut(s) | 199, 226 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 94, 108 |
| BstNI | CCWGG | 1 cut(s) | 76 |
| BstSCI | CCNGG | 2 cut(s) | 34, 74 |
| Bsu15I | ATCGAT | 1 cut(s) | 222 |
| BsuRI | GGCC | 1 cut(s) | 163 |
| BsuTUI | ATCGAT | 1 cut(s) | 222 |
| BtsCI | GGATG | 2 cut(s) | 164, 220 |
| BtsIMutI | CAGTG | 1 cut(s) | 209 |
| Cac8I | GCNNGC | 1 cut(s) | 86 |
| Cfr10I | RCCGGY | 1 cut(s) | 93 |
| Cfr13I | GGNCC | 2 cut(s) | 72, 142 |
| ClaI | ATCGAT | 1 cut(s) | 222 |
| CsiI | ACCWGGT | 1 cut(s) | 74 |
| CviJI | RGCY | 2 cut(s) | 97, 163 |
| CviKI_1 | RGCY | 2 cut(s) | 97, 163 |
| DdeI | CTNAG | 1 cut(s) | 98 |
| DpnI | GATC | 2 cut(s) | 221, 225 |
| DpnII | GATC | 2 cut(s) | 219, 223 |
| Eco47I | GGWCC | 2 cut(s) | 72, 142 |
| EcoO109I | RGGNCCY | 1 cut(s) | 142 |
| EcoRII | CCWGG | 1 cut(s) | 74 |
| FaiI | YATR | 2 cut(s) | 12, 90 |
| FokI | GGATG | 2 cut(s) | 151, 227 |
| HaeIII | GGCC | 1 cut(s) | 163 |
| HapII | CCGG | 3 cut(s) | 35, 94, 199 |
| HinfI | GANTC | 1 cut(s) | 49 |
| HpaII | CCGG | 3 cut(s) | 35, 94, 199 |
| Hpy166II | GTNNAC | 2 cut(s) | 131, 232 |
| Hpy188III | TCNNGA | 2 cut(s) | 53, 157 |
| Hpy8I | GTNNAC | 2 cut(s) | 131, 232 |
| HpyAV | CCTTC | 1 cut(s) | 133 |
| HpyCH4IV | ACGT | 1 cut(s) | 133 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 94, 108 |
| HpyF3I | CTNAG | 1 cut(s) | 98 |
| HpySE526I | ACGT | 1 cut(s) | 133 |
| Kzo9I | GATC | 2 cut(s) | 219, 223 |
| LmnI | GCTCC | 1 cut(s) | 201 |
| LpnPI | CCDG | 7 cut(s) | 48, 61, 88, 107, 142, 197, 212 |
| MabI | ACCWGGT | 1 cut(s) | 74 |
| MaeII | ACGT | 1 cut(s) | 133 |
| MalI | GATC | 2 cut(s) | 221, 225 |
| MboI | GATC | 2 cut(s) | 219, 223 |
| MluCI | AATT | 2 cut(s) | 56, 237 |
| MlyI | GAGTC | 1 cut(s) | 58 |
| MnlI | CCTC | 3 cut(s) | 76, 153, 178 |
| MspI | CCGG | 3 cut(s) | 35, 94, 199 |
| MspR9I | CCNGG | 2 cut(s) | 36, 76 |
| MvaI | CCWGG | 1 cut(s) | 76 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 94, 108 |
| NciI | CCSGG | 1 cut(s) | 36 |
| NdeII | GATC | 2 cut(s) | 219, 223 |
| NlaIV | GGNNCC | 1 cut(s) | 144 |
| NspV | TTCGAA | 1 cut(s) | 241 |
| Ple19I | CGATCG | 1 cut(s) | 226 |
| PleI | GAGTC | 1 cut(s) | 57 |
| PpsI | GAGTC | 1 cut(s) | 57 |
| PpuMI | RGGWCCY | 1 cut(s) | 142 |
| Psp5II | RGGWCCY | 1 cut(s) | 142 |
| Psp6I | CCWGG | 1 cut(s) | 74 |
| PspGI | CCWGG | 1 cut(s) | 74 |
| PspN4I | GGNNCC | 1 cut(s) | 144 |
| PspPI | GGNCC | 2 cut(s) | 72, 142 |
| PspPPI | RGGWCCY | 1 cut(s) | 142 |
| PvuI | CGATCG | 1 cut(s) | 226 |
| Sau3AI | GATC | 2 cut(s) | 219, 223 |
| Sau96I | GGNCC | 2 cut(s) | 72, 142 |
| SchI | GAGTC | 1 cut(s) | 58 |
| ScrFI | CCNGG | 2 cut(s) | 36, 76 |
| SetI | ASST | 3 cut(s) | 68, 80, 136 |
| SexAI | ACCWGGT | 1 cut(s) | 74 |
| SfuI | TTCGAA | 1 cut(s) | 241 |
| SinI | GGWCC | 2 cut(s) | 72, 142 |
| SmlI | CTYRAG | 1 cut(s) | 67 |
| SmoI | CTYRAG | 1 cut(s) | 67 |
| Sse9I | AATT | 2 cut(s) | 56, 237 |
| StyD4I | CCNGG | 2 cut(s) | 34, 74 |
| TaiI | ACGT | 1 cut(s) | 136 |
| TaqI | TCGA | 4 cut(s) | 7, 195, 222, 241 |
| TasI | AATT | 2 cut(s) | 56, 237 |
| TscAI | CASTG | 1 cut(s) | 216 |
| TspGWI | ACGGA | 1 cut(s) | 160 |
| TspRI | CASTG | 1 cut(s) | 216 |
| VpaK11BI | GGWCC | 2 cut(s) | 72, 142 |
| XapI | RAATTY | 1 cut(s) | 56 |