Rh6DG177600

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000458
Physical Location & Seq
Reverse (-)
31221011 .. 31229670
8660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG177600.1

Sequence Viewer

Length: 1233 bp
ATGGGTGGGATTTTCAACAAGGTCAAACGGACGCCCAAAGTAGAAGATGTCACTGTAGCTATAAATCGTCATCCTGCTAGTAATGGGTGTGATGAAATGCCCCCAAGAGCAAATGGTATTACAACTCCATCCAATTGCTTCTCTAAGCAGGCAGCTGTTGCTAAGGTTTCAGCAGTAAGCTCTCTTTTGGGTAAAGCTAGCTCGATTGGATTTGGGAAGTTAATGGAAATTTTGGACAGTTTTGGCACTAGCATCACAAATTGGGACCCCACTTTAAATTCTGAGGTGCAAACAAAAGGGACTACAATTTCAATTTTGGCTTTTGAAGTTGCAAACTCAGTTGTCAAGGGTGCGAAGCTAATGCAGTCCCTTTCAGAAGATGAGATCAGATATTTAAAAGACGTGCTGCTACCATCCAAAGGGGTGCAAAGTTTAATATCTACAGATATGGATGAGCTCCTCAGAATTGCCGCTGCTGACAAGAGAGAAGAGTTGAGTACTTTTTCGAGAGATGTTGTACGTTTTGGAAATCATATTAAGGATCCGACATGGCACAACTTGGATCGCTATTTTAGGAAGACTGACTTGAAAGTTACTGAACAAACTTATTTAAAGAAGCAAGCAGAGAGAGGGATGGAACATTTGATAACTCTAGTTCAGTCAACAGCAGAACTATGTCATGAGTTGCATTCGTTGGATAGATTGGAACGAGAATATCGGCATAAGCTTGAAGAAGATAGTTCAGTTACTGTTAAAAGAGGTAACCTTGTCTTTGGAGACAACCTTGCAATTTTAAACGCAGATATAAAGAATCAAATGAAGCTTGTAAACAGCTTAAAGAAAAAATCACTGTGGTCAAAGACTTTTGAAGAGGTTATGCCGAAGCTTGTAGACATTGTGCAATTATTACACTTCAAAATTCATGAAGCTTTTGGAAGTACTGATGATGACGAACCAGTGGAACGTTCTGAGAGGAACTGTAAAAGGTTGGGGCCTGCTGGTACTTCATTACACTATGCGCATATTATTACACAAATTAACACACTTGTGTCTCGATCAAGGTCAGTGCCTCAAAGTGTGATAGATACTCTGTATGGGGGACTGCCATGCAGTGTAAAGTCAACTCTGCGCTCAAAATTTCAGTTAGTTGAGCTCACCATTCCTCAAATCAAAGCTGAAATGAAGCAAACTTTGGATTGGCTTGTTCCCATCGCCATTAACACAACCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

410

Amino Acids

45.85

Weight (kDa)

8.76

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 105 - 161 2.3e-22 Domain of unknown function (DUF3475)
DUF668 PF05003 329 - 410 1.6e-21 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34320 AT1G34320
fragaria_vesca FvH4_2g12340 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_5g36070 FvH4_5g36100 FvH4_5g36130 FvH4_5g36140
malus_domestica MD05G1039900.v1.1 MD10G1045400.v1.1 MD10G1045600.v1.1 MD10G1045700.v1.1 MD10G1045800.v1.1 MD12G1057900.v1.1
prunus_persica Prupe.1G525500_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1
pyrus_communis pycom05g03250 pycom10g03180 pycom10g03190 pycom15g33960
rosa_chinensis RchiOBHm_Chr3g0484671 RchiOBHm_Chr6g0272351 RchiOBHm_Chr6g0272361 RchiOBHm_Chr7g0237881
rosa_laevigata RLG00000000952 RLG00000000960 RLG00000001025 RLG00000008689 RLG00000010609 RLG00000013679 RLG00000013680
rosa_multiflora Rmu_sc0001339.1_g000015 Rmu_sc0002877.1_g000020 Rmu_sc0009379.1_g000003 Rmu_ssc0000199.1_g000001 Rmu_ssc0000321.1_g000001
rosa_roxburghii Rroxscaffold_1G00039160 Rroxscaffold_3G00223500 Rroxscaffold_3G00223670 Rroxscaffold_7G00196370 Rroxscaffold_7G00196380
rosa_rugosa Rorug06G0068200 Rorug06G0068300 Rorug06G0068400 Rorug07G0302100 Rorug07G0308600 Rorug07G0309200.1
rosa_samantha Rh3AG263300 Rh3DG292000 Rh6AG113700 Rh6AG184400 Rh6AG184500 Rh6BG187800 Rh6BG187900 Rh6CG185900 Rh6DG177600 Rh6DG177700 Rh7AG458500 Rh7AG464200 Rh7AG464500 Rh7BG429200 Rh7BG434200 Rh7CG476400 Rh7CG481600 Rh7CG482700 Rh7DG445700 Rh7DG450300 Rh7DG451400
rosa_wichuraiana Rw0G019450 Rw6G015900 Rw6G015910 Rw6G015940 Rw6G015950 Rw7G037980 Rw7G038410 Rw7G038470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1020
AccI GTMKAC 1 cut(s) 891
AciI CCGC 1 cut(s) 471
AclI AACGTT 1 cut(s) 964
AclWI GGATC 3 cut(s) 536, 549, 570
AcsI RAATTY 4 cut(s) 228, 277, 918, 1136
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 4 cut(s) 499, 519, 940, 1003
AfiI CCNNNNNNNGG 1 cut(s) 419
AgsI TTSAA 7 cut(s) 16, 312, 326, 589, 731, 869, 916
AjiI CACGTC 1 cut(s) 403
AjuI GAANNNNNNNTTGG 2 cut(s) 1175, 1207
AleI CACNNNNGTG 1 cut(s) 1046
Alw21I GWGCWC 2 cut(s) 459, 1155
Alw26I GTCTC 2 cut(s) 771, 1056
AlwI GGATC 3 cut(s) 536, 549, 570
AlwNI CAGNNNCTG 1 cut(s) 749
AoxI GGCC 1 cut(s) 992
ApeKI GCWGC 3 cut(s) 152, 406, 473
ApoI RAATTY 4 cut(s) 228, 277, 918, 1136
AspLEI GCGC 2 cut(s) 1021, 1131
AspS9I GGNCC 2 cut(s) 265, 992
AsuHPI GGTGA 1 cut(s) 1147
AsuNHI GCTAGC 1 cut(s) 197
AvaII GGWCC 1 cut(s) 265
BamHI GGATCC 1 cut(s) 541
BanII GRGCYC 2 cut(s) 459, 1155
BbsI GAAGAC 1 cut(s) 584
Bbv12I GWGCWC 2 cut(s) 459, 1155
BbvI GCAGC 3 cut(s) 164, 393, 460
BccI CCATC 4 cut(s) 136, 421, 628, 1217
BcgI CGANNNNNNTGC 2 cut(s) 343, 377
BcoDI GTCTC 2 cut(s) 771, 1056
BfaI CTAG 4 cut(s) 78, 198, 249, 653
BfmI CTRYAG 2 cut(s) 54, 441
BisI GCNGC 4 cut(s) 153, 407, 471, 474
BlsI GCNGC 4 cut(s) 154, 408, 472, 475
BmcAI AGTACT 2 cut(s) 499, 940
Bme18I GGWCC 1 cut(s) 265
BmgBI CACGTC 1 cut(s) 403
BmgT120I GGNCC 2 cut(s) 265, 992
BmiI GGNNCC 4 cut(s) 266, 267, 543, 993
BmsI GCATC 1 cut(s) 261
BmtI GCTAGC 1 cut(s) 201
BpiI GAAGAC 1 cut(s) 584
Bpu10I CCTNAGC 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 1 cut(s) 419
Bse1I ACTGG 1 cut(s) 956
BseGI GGATG 5 cut(s) 70, 128, 413, 457, 639
BseLI CCNNNNNNNGG 1 cut(s) 419
BseMII CTCAG 4 cut(s) 273, 351, 475, 960
BseNI ACTGG 1 cut(s) 956
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 3 cut(s) 164, 393, 460
BshFI GGCC 1 cut(s) 994
BsiHKAI GWGCWC 2 cut(s) 459, 1155
BslFI GGGAC 4 cut(s) 278, 313, 352, 1113
BslI CCNNNNNNNGG 1 cut(s) 419
BsmAI GTCTC 2 cut(s) 771, 1056
BsmFI GGGAC 4 cut(s) 278, 313, 352, 1113
BsmI GAATGC 1 cut(s) 688
BsnI GGCC 1 cut(s) 994
Bsp1286I GDGCHC 2 cut(s) 459, 1155
Bsp143I GATC 4 cut(s) 384, 541, 562, 1055
BspACI CCGC 1 cut(s) 471
BspANI GGCC 1 cut(s) 994
BspCNI CTCAG 4 cut(s) 274, 350, 474, 961
BspHI TCATGA 2 cut(s) 679, 922
BspLI GGNNCC 4 cut(s) 266, 267, 543, 993
BspOI GCTAGC 1 cut(s) 201
BspPI GGATC 3 cut(s) 536, 549, 570
BsrI ACTGG 1 cut(s) 956
BssMI GATC 4 cut(s) 384, 541, 562, 1055
BssNI GRCGYC 1 cut(s) 32
Bst4CI ACNGT 5 cut(s) 55, 239, 751, 852, 980
Bst6I CTCTTC 2 cut(s) 483, 864
BstACI GRCGYC 1 cut(s) 32
BstAPI GCANNNNNTGC 1 cut(s) 158
BstC8I GCNNGC 4 cut(s) 150, 199, 621, 996
BstDEI CTNAG 6 cut(s) 144, 162, 282, 337, 461, 969
BstEII GGTNACC 1 cut(s) 761
BstF5I GGATG 5 cut(s) 70, 128, 413, 457, 639
BstHHI GCGC 2 cut(s) 1021, 1131
BstKTI GATC 4 cut(s) 387, 544, 565, 1058
BstMAI GTCTC 2 cut(s) 771, 1056
BstMBI GATC 4 cut(s) 384, 541, 562, 1055
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstPI GGTNACC 1 cut(s) 761
BstSFI CTRYAG 2 cut(s) 54, 441
BstV1I GCAGC 3 cut(s) 164, 393, 460
BstV2I GAAGAC 1 cut(s) 584
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
BsuRI GGCC 1 cut(s) 994
BtgZI GCGATG 1 cut(s) 1195
BtrI CACGTC 1 cut(s) 403
BtsCI GGATG 5 cut(s) 70, 128, 413, 457, 639
BtsI GCAGTG 1 cut(s) 1117
BtsIMutI CAGTG 5 cut(s) 51, 848, 963, 1071, 1117
Cac8I GCNNGC 4 cut(s) 150, 199, 621, 996
CaiI CAGNNNCTG 1 cut(s) 749
CciI TCATGA 2 cut(s) 679, 922
CfoI GCGC 2 cut(s) 1021, 1131
Cfr13I GGNCC 2 cut(s) 265, 992
CseI GACGC 1 cut(s) 40
Csp6I GTAC 4 cut(s) 498, 518, 939, 1002
CviAII CATG 4 cut(s) 549, 680, 923, 1107
CviQI GTAC 4 cut(s) 498, 518, 939, 1002
DdeI CTNAG 6 cut(s) 144, 162, 282, 337, 461, 969
DpnI GATC 4 cut(s) 386, 543, 564, 1057
DpnII GATC 4 cut(s) 384, 541, 562, 1055
DraI TTTAAA 4 cut(s) 276, 396, 612, 795
Eam1104I CTCTTC 2 cut(s) 483, 864
EarI CTCTTC 2 cut(s) 483, 864
Ecl136II GAGCTC 2 cut(s) 457, 1153
Eco24I GRGCYC 2 cut(s) 459, 1155
Eco47I GGWCC 1 cut(s) 265
Eco53kI GAGCTC 2 cut(s) 457, 1153
Eco91I GGTNACC 1 cut(s) 761
EcoICRI GAGCTC 2 cut(s) 457, 1153
EcoO109I RGGNCCY 2 cut(s) 265, 992
EcoO65I GGTNACC 1 cut(s) 761
EcoT38I GRGCYC 2 cut(s) 459, 1155
FaeI CATG 4 cut(s) 552, 683, 926, 1110
FalI AAGNNNNNCTT 2 cut(s) 569, 601
FaqI GGGAC 4 cut(s) 278, 313, 352, 1113
FatI CATG 4 cut(s) 548, 679, 922, 1106
FblI GTMKAC 1 cut(s) 891
Fnu4HI GCNGC 4 cut(s) 153, 407, 471, 474
FokI GGATG 5 cut(s) 57, 115, 400, 464, 646
FriOI GRGCYC 2 cut(s) 459, 1155
Fsp4HI GCNGC 4 cut(s) 153, 407, 471, 474
FspAI RTGCGCAY 1 cut(s) 1020
FspBI CTAG 4 cut(s) 78, 198, 249, 653
FspI TGCGCA 1 cut(s) 1020
GlaI GCGC 2 cut(s) 1020, 1130
GluI GCNGC 4 cut(s) 153, 407, 471, 474
HaeIII GGCC 1 cut(s) 994
HgaI GACGC 1 cut(s) 40
HhaI GCGC 2 cut(s) 1021, 1131
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 4 cut(s) 552, 683, 926, 1110
Hin6I GCGC 2 cut(s) 1019, 1129
HinP1I GCGC 2 cut(s) 1019, 1129
HincII GTYRAC 2 cut(s) 663, 1122
HindII GTYRAC 2 cut(s) 663, 1122
HindIII AAGCTT 4 cut(s) 725, 821, 884, 927
HinfI GANTC 1 cut(s) 811
HphI GGTGA 1 cut(s) 1147
Hpy166II GTNNAC 4 cut(s) 663, 829, 892, 1122
Hpy188I TCNGA 6 cut(s) 283, 376, 389, 464, 546, 970
Hpy188III TCNNGA 4 cut(s) 507, 680, 923, 1053
Hpy8I GTNNAC 4 cut(s) 663, 829, 892, 1122
HpyCH4III ACNGT 5 cut(s) 55, 239, 751, 852, 980
HpyCH4IV ACGT 3 cut(s) 402, 520, 964
HpyCH4V TGCA 8 cut(s) 289, 332, 364, 427, 688, 788, 901, 1110
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 6 cut(s) 144, 162, 282, 337, 461, 969
HpySE526I ACGT 3 cut(s) 402, 520, 964
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 4 cut(s) 552, 683, 926, 1110
HspAI GCGC 2 cut(s) 1019, 1129
KflI GGGWCCC 1 cut(s) 265
Kzo9I GATC 4 cut(s) 384, 541, 562, 1055
LmnI GCTCC 1 cut(s) 462
LpnPI CCDG 5 cut(s) 87, 134, 969, 984, 1008
Lsp1109I GCAGC 3 cut(s) 164, 393, 460
LweI GCATC 1 cut(s) 261
MaeI CTAG 4 cut(s) 78, 198, 249, 653
MaeII ACGT 3 cut(s) 402, 520, 964
MaeIII GTNAC 4 cut(s) 49, 592, 745, 761
MalI GATC 4 cut(s) 386, 543, 564, 1057
MboI GATC 4 cut(s) 384, 541, 562, 1055
MboII GAAGA 7 cut(s) 56, 389, 500, 589, 743, 746, 881
MfeI CAATTG 1 cut(s) 133
MflI RGATCY 1 cut(s) 541
MhlI GDGCHC 2 cut(s) 459, 1155
MmeI TCCRAC 2 cut(s) 569, 675
MnlI CCTC 8 cut(s) 277, 470, 623, 752, 865, 966, 1080, 1173
MslI CAYNNNNRTG 1 cut(s) 1046
MspA1I CMGCKG 2 cut(s) 155, 473
MunI CAATTG 1 cut(s) 133
Mva1269I GAATGC 1 cut(s) 688
MwoI GCNNNNNNNGC 1 cut(s) 158
NdeII GATC 4 cut(s) 384, 541, 562, 1055
NheI GCTAGC 1 cut(s) 197
NlaIII CATG 4 cut(s) 552, 683, 926, 1110
NlaIV GGNNCC 4 cut(s) 266, 267, 543, 993
NmuCI GTSAC 1 cut(s) 49
NsbI TGCGCA 1 cut(s) 1020
OliI CACNNNNGTG 1 cut(s) 1046
PagI TCATGA 2 cut(s) 679, 922
PctI GAATGC 1 cut(s) 688
PfeI GAWTC 1 cut(s) 811
PkrI GCNGC 4 cut(s) 154, 408, 472, 475
PpuMI RGGWCCY 1 cut(s) 265
Psp124BI GAGCTC 2 cut(s) 459, 1155
Psp1406I AACGTT 1 cut(s) 964
Psp5II RGGWCCY 1 cut(s) 265
PspEI GGTNACC 1 cut(s) 761
PspN4I GGNNCC 4 cut(s) 266, 267, 543, 993
PspPI GGNCC 2 cut(s) 265, 992
PspPPI RGGWCCY 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 1 cut(s) 541
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 4 cut(s) 499, 519, 940, 1003
RsaNI GTAC 4 cut(s) 498, 518, 939, 1002
RseI CAYNNNNRTG 1 cut(s) 1046
SacI GAGCTC 2 cut(s) 459, 1155
SatI GCNGC 4 cut(s) 153, 407, 471, 474
Sau3AI GATC 4 cut(s) 384, 541, 562, 1055
Sau96I GGNCC 2 cut(s) 265, 992
ScaI AGTACT 2 cut(s) 499, 940
SduI GDGCHC 2 cut(s) 459, 1155
SfaNI GCATC 1 cut(s) 261
SfcI CTRYAG 2 cut(s) 54, 441
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 1046
SsiI CCGC 1 cut(s) 471
SspMI CTAG 4 cut(s) 78, 198, 249, 653
SstI GAGCTC 2 cut(s) 459, 1155
TaaI ACNGT 5 cut(s) 55, 239, 751, 852, 980
TaiI ACGT 3 cut(s) 405, 523, 967
TaqI TCGA 3 cut(s) 203, 506, 1054
TatI WGTACW 2 cut(s) 497, 938
TauI GCSGC 1 cut(s) 473
TfiI GAWTC 1 cut(s) 811
TscAI CASTG 5 cut(s) 58, 855, 963, 1071, 1117
TseFI GTSAC 1 cut(s) 49
TseI GCWGC 3 cut(s) 152, 406, 473
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 6 cut(s) 108, 833, 911, 939, 996, 1196
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 5 cut(s) 58, 855, 963, 1071, 1117
VpaK11BI GGWCC 1 cut(s) 265
XapI RAATTY 4 cut(s) 228, 277, 918, 1136
XmiI GTMKAC 1 cut(s) 891
XspI CTAG 4 cut(s) 78, 198, 249, 653
ZrmI AGTACT 2 cut(s) 499, 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.