Rroxscaffold_7G00196380

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000458
Physical Location & Seq
Forward (+)
39242884 .. 39252435
9552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00196380.1

Sequence Viewer

Length: 1884 bp
ATGGGTGGGATTATCAACAGTGTCAAACGGACGCCCAAAGTAGAAGATGTCACTGTGGCTATAAATGATCATTATGCTAGTAATGGGTGTGATGAACTGCCCCCAAGAGCGAATGGTATTACAACTCCATCCAATTGTTTCTCTAGGCAGGCAGCTGTTGCTAAGTCATCAGTTTTGATAGGAATATTGAAAATTCTACCGGTTGTTGAACATATAAGTAATGTCATCATACTACAGGTTTCAGCAGTAAGCTCTCTTTTGGGTAAAGCTAGCTCAATTGGATTTGGGAAGGCAATGGAAATTTTGGACAGTTTTGGCAGTAGCATCACAAATTGGGACCCCACTTTAAATTCTGAGGTGCAAATAAAAGGGAATACAATTTCAATTTTGGCTTTTGAAGTTGCAAACTCAGTTGTCAAGGGTGCGAAGCTAATGCAGTCCCTTTCAGAAGAGGAGATCAGATATTTAAAAGACGTGCTGCTACCATCCAAAGGGGTGCAAAGTTTAATATCTACAGATATGGATGAGCTCCTCAGAATTGTCGCTGCTGACAAGAGGACTGGCTTGAAAGTTACTGAACAAACTCATTTAAAGAAGCAAGCAGAGGGAGGGATGGAACATTTGATAACTCTAGTTCAGTCAACAGCAGAACTATGTCATGAGTTGCATTCGTTGGATAGATTGGAAAAAGAATATCGGCATAAGCTTGAAGAAGATAGTTCAGTTACTGCTAGAAGAGGAGACAACCTTGCAATTTTAAATGCAGATATAAAGAATCAATTGAAGCTTGTAAACAGCTTAAAGAAAAAATCACTGTGGTCAAAGACTTTTGAAGAGGTTATGCCGAAGCTTGTAGACATTGTGCAATTATTACACTTAAAAATTCATGAAGCTTTTGGAAGTACTGATGATGACGAACCAGTGGAACGTTCTCAGAGGAACTGTAAAAGTTCGGGGCCTGCTGGTACTTCATTACACTATGCGCATATTATTACACAAATTAACACACTTGTGTCTCGATCAAGGTCAGTGCCTCAAAGTGTAATAGATACTCTTTATGGGGGACTGCCATGCAGTGTAAAGTCAACTCTGCGCTCAAAATTTCATTTAGTTGAGCTCACCATTCCTCAAATCAAAGCTGAAATGAAGCAAACTTTGGATTGGCTTGTTCCCATCGCCATTAACACAACCAAGTTTGAGGCAAGCTGGCATACTGGCTTGACTAGCATTCCGATGATTGGGACTCTACACCATGCCGATGAGAAAAAAACTGAAGCTTATATATGTGAACTAGTAGTCTTGCTACATTATCTGATCAGCCAGGCGGGGGTTGGCCATGGTGGAATGCCATCGTCACCTGTTGAAACCCGCAGCCAGAGGACGAATGAGTTGACAGCTGAAGATCAAGAGATGCTTAAGGATGTGGGTATAAACAGCATCAGACTAGGAATGAGTAAAAGTCAGAATTTTGACGCAGGAAAGGCCAGCAGGTTGAGCAGACACCATAGGCTGAGTAAGAGTAGCAGAGACTTCCTAGCAAAAGATAAACTTCCCATTGAGAATCTGTTGTCTAGGCCCATAGACTCTGATATAACCTGGACCAAGTTCTTGGATGTCATTGATGGAGTTGACACAATGCGCAGCAACTTCATGGTTGTTTCCACCTTCTACATCCACAAATGCTGCGTCGACCAAGTCAACCGCTGCCTCATAGAGATCCGCCGCAAGAGTGGCCGTGAGGGTTTGGGTTTGGACTGGGAGACATCGGAAAATGACGTGGTCCAGGTCCTTGGACGAAAAATCAATTCCCTCCTACAGAATCTCATCCTCGCTGCCCAATCCGAATATGAGAAGAGGCGGTTTCATCGAGGAGACCATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 ko05164 map03013 map03015 map03040 map05164
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

627

Amino Acids

69.6

Weight (kDa)

7.67

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 129 - 185 5.9e-22 Domain of unknown function (DUF3475)
DUF668 PF05003 319 - 398 7.3e-20 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34320 AT1G34320
fragaria_vesca FvH4_2g12340 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_2g12350 FvH4_5g36070 FvH4_5g36100 FvH4_5g36130 FvH4_5g36140
malus_domestica MD05G1039900.v1.1 MD10G1045400.v1.1 MD10G1045600.v1.1 MD10G1045700.v1.1 MD10G1045800.v1.1 MD12G1057900.v1.1
prunus_persica Prupe.1G525500_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052300_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1 Prupe.8G052600_v2.0.a1
pyrus_communis pycom05g03250 pycom10g03180 pycom10g03190 pycom15g33960
rosa_chinensis RchiOBHm_Chr3g0484671 RchiOBHm_Chr6g0272351 RchiOBHm_Chr6g0272361 RchiOBHm_Chr7g0237881
rosa_laevigata RLG00000000952 RLG00000000960 RLG00000001025 RLG00000008689 RLG00000010609 RLG00000013679 RLG00000013680
rosa_multiflora Rmu_sc0001339.1_g000015 Rmu_sc0002877.1_g000020 Rmu_sc0009379.1_g000003 Rmu_ssc0000199.1_g000001 Rmu_ssc0000321.1_g000001
rosa_roxburghii Rroxscaffold_1G00039160 Rroxscaffold_3G00223500 Rroxscaffold_3G00223670 Rroxscaffold_7G00196370 Rroxscaffold_7G00196380
rosa_rugosa Rorug06G0068200 Rorug06G0068300 Rorug06G0068400 Rorug07G0302100 Rorug07G0308600 Rorug07G0309200.1
rosa_samantha Rh3AG263300 Rh3DG292000 Rh6AG113700 Rh6AG184400 Rh6AG184500 Rh6BG187800 Rh6BG187900 Rh6CG185900 Rh6DG177600 Rh6DG177700 Rh7AG458500 Rh7AG464200 Rh7AG464500 Rh7BG429200 Rh7BG434200 Rh7CG476400 Rh7CG481600 Rh7CG482700 Rh7DG445700 Rh7DG450300 Rh7DG451400
rosa_wichuraiana Rw0G019450 Rw6G015900 Rw6G015910 Rw6G015940 Rw6G015950 Rw7G037980 Rw7G038410 Rw7G038470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 984, 1640
Acc36I ACCTGC 1 cut(s) 1479
AccI GTMKAC 2 cut(s) 855, 1689
AciI CCGC 6 cut(s) 1325, 1369, 1702, 1720, 1723, 1858
AclI AACGTT 1 cut(s) 928
AclWI GGATC 1 cut(s) 1711
AcoI YGGCCR 2 cut(s) 1333, 1732
AcsI RAATTY 6 cut(s) 192, 300, 349, 882, 1100, 1465
AcuI CTGAAG 2 cut(s) 1293, 1419
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 2 cut(s) 904, 967
AfiI CCNNNNNNNGG 3 cut(s) 491, 1238, 1327
AflII CTTAAG 1 cut(s) 1415
AgeI ACCGGT 1 cut(s) 199
AgsI TTSAA 9 cut(s) 190, 209, 384, 398, 568, 710, 784, 833, 1364
AhlI ACTAGT 1 cut(s) 1291
AjiI CACGTC 2 cut(s) 475, 1777
AjnI CCWGG 3 cut(s) 1320, 1595, 1782
AjuI GAANNNNNNNTTGG 2 cut(s) 1139, 1171
AleI CACNNNNGTG 1 cut(s) 1010
Alw21I GWGCWC 2 cut(s) 531, 1119
Alw26I GTCTC 5 cut(s) 735, 1020, 1521, 1754, 1866
AlwI GGATC 1 cut(s) 1711
AlwNI CAGNNNCTG 1 cut(s) 728
AoxI GGCC 5 cut(s) 956, 1333, 1482, 1574, 1732
ApeKI GCWGC 8 cut(s) 152, 478, 545, 1371, 1641, 1683, 1704, 1832
ApoI RAATTY 6 cut(s) 192, 300, 349, 882, 1100, 1465
ArsI GACNNNNNNTTYG 2 cut(s) 1671, 1703
AsiGI ACCGGT 1 cut(s) 199
AspLEI GCGC 3 cut(s) 985, 1095, 1641
AspS9I GGNCC 6 cut(s) 337, 956, 1575, 1599, 1780, 1786
AsuHPI GGTGA 2 cut(s) 1111, 1347
AsuNHI GCTAGC 1 cut(s) 269
AvaII GGWCC 4 cut(s) 337, 1599, 1780, 1786
BalI TGGCCA 1 cut(s) 1335
BanII GRGCYC 2 cut(s) 531, 1119
Bbv12I GWGCWC 2 cut(s) 531, 1119
BbvI GCAGC 8 cut(s) 164, 465, 532, 1383, 1653, 1670, 1691, 1819
BccI CCATC 6 cut(s) 136, 493, 607, 1181, 1357, 1616
BceAI ACGGC 1 cut(s) 1719
BcgI CGANNNNNNTGC 2 cut(s) 415, 449
BciT130I CCWGG 3 cut(s) 1322, 1597, 1784
BclI TGATCA 2 cut(s) 67, 1314
BcoDI GTCTC 5 cut(s) 735, 1020, 1521, 1754, 1866
BcuI ACTAGT 1 cut(s) 1291
BfmI CTRYAG 3 cut(s) 233, 513, 1814
BfrI CTTAAG 1 cut(s) 1415
BfuAI ACCTGC 1 cut(s) 1479
BisI GCNGC 9 cut(s) 153, 479, 546, 1372, 1642, 1684, 1705, 1723, 1833
BlsI GCNGC 9 cut(s) 154, 480, 547, 1373, 1643, 1685, 1706, 1724, 1834
BmcAI AGTACT 1 cut(s) 904
Bme1390I CCNGG 3 cut(s) 1322, 1597, 1784
Bme18I GGWCC 4 cut(s) 337, 1599, 1780, 1786
BmgBI CACGTC 2 cut(s) 475, 1777
BmgT120I GGNCC 6 cut(s) 337, 956, 1575, 1599, 1780, 1786
BmiI GGNNCC 3 cut(s) 338, 339, 957
BmrFI CCNGG 3 cut(s) 1322, 1597, 1784
BmrI ACTGGG 1 cut(s) 1765
BmsI GCATC 3 cut(s) 333, 1401, 1446
BmtI GCTAGC 1 cut(s) 273
BmuI ACTGGG 1 cut(s) 1765
BsaHI GRCGYC 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 1866
BsaJI CCNNGG 2 cut(s) 1336, 1789
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 1 cut(s) 1863
Bsc4I CCNNNNNNNGG 3 cut(s) 491, 1238, 1327
Bse118I RCCGGY 1 cut(s) 199
Bse1I ACTGG 4 cut(s) 565, 920, 1219, 1760
Bse3DI GCAATG 1 cut(s) 300
BseBI CCWGG 3 cut(s) 1322, 1597, 1784
BseDI CCNNGG 2 cut(s) 1336, 1789
BseGI GGATG 8 cut(s) 128, 485, 529, 618, 1426, 1618, 1671, 1824
BseLI CCNNNNNNNGG 3 cut(s) 491, 1238, 1327
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 5 cut(s) 345, 423, 547, 947, 1502
BseNI ACTGG 4 cut(s) 565, 920, 1219, 1760
BseRI GAGGAG 4 cut(s) 467, 521, 753, 1884
BseXI GCAGC 8 cut(s) 164, 465, 532, 1383, 1653, 1670, 1691, 1819
BshFI GGCC 5 cut(s) 958, 1335, 1484, 1576, 1734
BshTI ACCGGT 1 cut(s) 199
BsiHKAI GWGCWC 2 cut(s) 531, 1119
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 4 cut(s) 350, 424, 1077, 1255
BslI CCNNNNNNNGG 3 cut(s) 491, 1238, 1327
BsmAI GTCTC 5 cut(s) 735, 1020, 1521, 1754, 1866
BsmFI GGGAC 4 cut(s) 350, 424, 1077, 1255
BsmI GAATGC 3 cut(s) 667, 1227, 1350
BsnI GGCC 5 cut(s) 958, 1335, 1484, 1576, 1734
Bso31I GGTCTC 1 cut(s) 1866
Bsp1286I GDGCHC 2 cut(s) 531, 1119
Bsp143I GATC 6 cut(s) 67, 456, 1019, 1314, 1402, 1716
Bsp19I CCATGG 1 cut(s) 1336
BspACI CCGC 6 cut(s) 1325, 1369, 1702, 1720, 1723, 1858
BspANI GGCC 5 cut(s) 958, 1335, 1484, 1576, 1734
BspCNI CTCAG 5 cut(s) 346, 422, 546, 946, 1503
BspHI TCATGA 2 cut(s) 658, 886
BspLI GGNNCC 3 cut(s) 338, 339, 957
BspMI ACCTGC 1 cut(s) 1479
BspOI GCTAGC 1 cut(s) 273
BspPI GGATC 1 cut(s) 1711
BspTI CTTAAG 1 cut(s) 1415
BspTNI GGTCTC 1 cut(s) 1866
BsrDI GCAATG 1 cut(s) 300
BsrFI RCCGGY 1 cut(s) 199
BsrI ACTGG 4 cut(s) 565, 920, 1219, 1760
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 2 cut(s) 1336, 1789
BssMI GATC 6 cut(s) 67, 456, 1019, 1314, 1402, 1716
BssNI GRCGYC 1 cut(s) 32
BssT1I CCWWGG 2 cut(s) 1336, 1789
Bst2UI CCWGG 3 cut(s) 1322, 1597, 1784
Bst4CI ACNGT 5 cut(s) 20, 55, 311, 816, 944
Bst6I CTCTTC 4 cut(s) 444, 730, 828, 1847
BstACI GRCGYC 1 cut(s) 32
BstAFI CTTAAG 1 cut(s) 1415
BstAPI GCANNNNNTGC 1 cut(s) 158
BstC8I GCNNGC 7 cut(s) 150, 271, 600, 960, 1204, 1208, 1486
BstDEI CTNAG 6 cut(s) 162, 354, 409, 533, 933, 1511
BstDSI CCRYGG 1 cut(s) 1336
BstF5I GGATG 8 cut(s) 128, 485, 529, 618, 1426, 1618, 1671, 1824
BstHHI GCGC 3 cut(s) 985, 1095, 1641
BstKTI GATC 6 cut(s) 70, 459, 1022, 1317, 1405, 1719
BstMAI GTCTC 5 cut(s) 735, 1020, 1521, 1754, 1866
BstMBI GATC 6 cut(s) 67, 456, 1019, 1314, 1402, 1716
BstMWI GCNNNNNNNGC 5 cut(s) 158, 1224, 1481, 1494, 1731
BstNI CCWGG 3 cut(s) 1322, 1597, 1784
BstSCI CCNGG 3 cut(s) 1320, 1595, 1782
BstSFI CTRYAG 3 cut(s) 233, 513, 1814
BstV1I GCAGC 8 cut(s) 164, 465, 532, 1383, 1653, 1670, 1691, 1819
BstX2I RGATCY 1 cut(s) 1716
BstXI CCANNNNNNTGG 2 cut(s) 1609, 1790
BstYI RGATCY 1 cut(s) 1716
BsuRI GGCC 5 cut(s) 958, 1335, 1484, 1576, 1734
BtgI CCRYGG 1 cut(s) 1336
BtgZI GCGATG 1 cut(s) 1159
BtrI CACGTC 2 cut(s) 475, 1777
BtsCI GGATG 8 cut(s) 128, 485, 529, 618, 1426, 1618, 1671, 1824
BtsI GCAGTG 1 cut(s) 1081
BtsIMutI CAGTG 6 cut(s) 25, 51, 812, 927, 1035, 1081
BveI ACCTGC 1 cut(s) 1479
Cac8I GCNNGC 7 cut(s) 150, 271, 600, 960, 1204, 1208, 1486
CaiI CAGNNNCTG 1 cut(s) 728
CciI TCATGA 2 cut(s) 658, 886
CfoI GCGC 3 cut(s) 985, 1095, 1641
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 6 cut(s) 337, 956, 1575, 1599, 1780, 1786
CseI GACGC 3 cut(s) 40, 1481, 1675
Csp6I GTAC 2 cut(s) 903, 966
CspAI ACCGGT 1 cut(s) 199
CviAII CATG 7 cut(s) 659, 887, 1071, 1253, 1337, 1651, 1877
CviQI GTAC 2 cut(s) 903, 966
DdeI CTNAG 6 cut(s) 162, 354, 409, 533, 933, 1511
DpnI GATC 6 cut(s) 69, 458, 1021, 1316, 1404, 1718
DpnII GATC 6 cut(s) 67, 456, 1019, 1314, 1402, 1716
DraI TTTAAA 4 cut(s) 348, 468, 591, 759
EaeI YGGCCR 2 cut(s) 1333, 1732
Eam1104I CTCTTC 4 cut(s) 444, 730, 828, 1847
EarI CTCTTC 4 cut(s) 444, 730, 828, 1847
EciI GGCGGA 1 cut(s) 1709
Ecl136II GAGCTC 2 cut(s) 529, 1117
Eco130I CCWWGG 2 cut(s) 1336, 1789
Eco24I GRGCYC 2 cut(s) 531, 1119
Eco31I GGTCTC 1 cut(s) 1866
Eco47I GGWCC 4 cut(s) 337, 1599, 1780, 1786
Eco53kI GAGCTC 2 cut(s) 529, 1117
Eco57I CTGAAG 2 cut(s) 1293, 1419
EcoICRI GAGCTC 2 cut(s) 529, 1117
EcoO109I RGGNCCY 3 cut(s) 337, 956, 1786
EcoRII CCWGG 3 cut(s) 1320, 1595, 1782
EcoT14I CCWWGG 2 cut(s) 1336, 1789
EcoT38I GRGCYC 2 cut(s) 531, 1119
ErhI CCWWGG 2 cut(s) 1336, 1789
FaeI CATG 7 cut(s) 662, 890, 1074, 1256, 1340, 1654, 1880
FalI AAGNNNNNCTT 4 cut(s) 1398, 1430, 1533, 1565
FaqI GGGAC 4 cut(s) 350, 424, 1077, 1255
FatI CATG 7 cut(s) 658, 886, 1070, 1252, 1336, 1650, 1876
FauI CCCGC 2 cut(s) 1318, 1376
FbaI TGATCA 2 cut(s) 67, 1314
FblI GTMKAC 2 cut(s) 855, 1689
Fnu4HI GCNGC 9 cut(s) 153, 479, 546, 1372, 1642, 1684, 1705, 1723, 1833
FokI GGATG 8 cut(s) 115, 472, 536, 625, 1433, 1625, 1658, 1811
FriOI GRGCYC 2 cut(s) 531, 1119
Fsp4HI GCNGC 9 cut(s) 153, 479, 546, 1372, 1642, 1684, 1705, 1723, 1833
FspAI RTGCGCAY 1 cut(s) 984
FspI TGCGCA 2 cut(s) 984, 1640
GlaI GCGC 3 cut(s) 984, 1094, 1640
GluI GCNGC 9 cut(s) 153, 479, 546, 1372, 1642, 1684, 1705, 1723, 1833
HaeIII GGCC 5 cut(s) 958, 1335, 1484, 1576, 1734
HapII CCGG 1 cut(s) 200
HgaI GACGC 3 cut(s) 40, 1481, 1675
HhaI GCGC 3 cut(s) 985, 1095, 1641
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 7 cut(s) 662, 890, 1074, 1256, 1340, 1654, 1880
Hin6I GCGC 3 cut(s) 983, 1093, 1639
HinP1I GCGC 3 cut(s) 983, 1093, 1639
HincII GTYRAC 6 cut(s) 642, 1086, 1392, 1630, 1690, 1699
HindII GTYRAC 6 cut(s) 642, 1086, 1392, 1630, 1690, 1699
HindIII AAGCTT 5 cut(s) 704, 785, 848, 891, 1275
HinfI GANTC 5 cut(s) 775, 1243, 1561, 1583, 1819
HpaII CCGG 1 cut(s) 200
HphI GGTGA 2 cut(s) 1111, 1347
Hpy166II GTNNAC 9 cut(s) 642, 793, 856, 1086, 1289, 1392, 1630, 1690, 1699
Hpy188III TCNNGA 4 cut(s) 659, 887, 1017, 1406
Hpy8I GTNNAC 9 cut(s) 642, 793, 856, 1086, 1289, 1392, 1630, 1690, 1699
Hpy99I CGWCG 1 cut(s) 1691
HpyAV CCTTC 2 cut(s) 283, 1675
HpyCH4III ACNGT 5 cut(s) 20, 55, 311, 816, 944
HpyCH4IV ACGT 3 cut(s) 474, 928, 1776
HpyCH4V TGCA 9 cut(s) 361, 404, 436, 499, 667, 752, 764, 865, 1074
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 1224, 1481, 1494, 1731
HpyF3I CTNAG 6 cut(s) 162, 354, 409, 533, 933, 1511
HpySE526I ACGT 3 cut(s) 474, 928, 1776
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 7 cut(s) 662, 890, 1074, 1256, 1340, 1654, 1880
HspAI GCGC 3 cut(s) 983, 1093, 1639
KflI GGGWCCC 1 cut(s) 337
Ksp22I TGATCA 2 cut(s) 67, 1314
Kzo9I GATC 6 cut(s) 67, 456, 1019, 1314, 1402, 1716
LmnI GCTCC 1 cut(s) 534
Lsp1109I GCAGC 8 cut(s) 164, 465, 532, 1383, 1653, 1670, 1691, 1819
LweI GCATC 3 cut(s) 333, 1401, 1446
MaeII ACGT 3 cut(s) 474, 928, 1776
MaeIII GTNAC 4 cut(s) 49, 571, 724, 1353
MalI GATC 6 cut(s) 69, 458, 1021, 1316, 1404, 1718
MboI GATC 6 cut(s) 67, 456, 1019, 1314, 1402, 1716
MboII GAAGA 8 cut(s) 56, 461, 722, 725, 747, 845, 1412, 1864
MfeI CAATTG 3 cut(s) 133, 276, 779
MflI RGATCY 1 cut(s) 1716
MhlI GDGCHC 2 cut(s) 531, 1119
MlsI TGGCCA 1 cut(s) 1335
MluNI TGGCCA 1 cut(s) 1335
MlyI GAGTC 2 cut(s) 1237, 1577
MmeI TCCRAC 1 cut(s) 654
Mox20I TGGCCA 1 cut(s) 1335
MscI TGGCCA 1 cut(s) 1335
MslI CAYNNNNRTG 3 cut(s) 1010, 1232, 1257
Msp20I TGGCCA 1 cut(s) 1335
MspA1I CMGCKG 3 cut(s) 155, 1397, 1704
MspCI CTTAAG 1 cut(s) 1415
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 3 cut(s) 1322, 1597, 1784
MunI CAATTG 3 cut(s) 133, 276, 779
Mva1269I GAATGC 3 cut(s) 667, 1227, 1350
MvaI CCWGG 3 cut(s) 1322, 1597, 1784
MwoI GCNNNNNNNGC 5 cut(s) 158, 1224, 1481, 1494, 1731
NcoI CCATGG 1 cut(s) 1336
NdeII GATC 6 cut(s) 67, 456, 1019, 1314, 1402, 1716
NheI GCTAGC 1 cut(s) 269
NlaIII CATG 7 cut(s) 662, 890, 1074, 1256, 1340, 1654, 1880
NlaIV GGNNCC 3 cut(s) 338, 339, 957
NmuCI GTSAC 2 cut(s) 49, 1353
NsbI TGCGCA 2 cut(s) 984, 1640
OliI CACNNNNGTG 1 cut(s) 1010
PagI TCATGA 2 cut(s) 658, 886
PctI GAATGC 3 cut(s) 667, 1227, 1350
PfeI GAWTC 3 cut(s) 775, 1561, 1819
PflFI GACNNNGTC 2 cut(s) 1694, 1778
PinAI ACCGGT 1 cut(s) 199
PkrI GCNGC 9 cut(s) 154, 480, 547, 1373, 1643, 1685, 1706, 1724, 1834
PleI GAGTC 2 cut(s) 1237, 1577
PpsI GAGTC 2 cut(s) 1237, 1577
PpuMI RGGWCCY 2 cut(s) 337, 1786
Psp124BI GAGCTC 2 cut(s) 531, 1119
Psp1406I AACGTT 1 cut(s) 928
Psp5II RGGWCCY 2 cut(s) 337, 1786
Psp6I CCWGG 3 cut(s) 1320, 1595, 1782
PspGI CCWGG 3 cut(s) 1320, 1595, 1782
PspN4I GGNNCC 3 cut(s) 338, 339, 957
PspPI GGNCC 6 cut(s) 337, 956, 1575, 1599, 1780, 1786
PspPPI RGGWCCY 2 cut(s) 337, 1786
PstNI CAGNNNCTG 1 cut(s) 728
PsuI RGATCY 1 cut(s) 1716
PsyI GACNNNGTC 2 cut(s) 1694, 1778
PvuII CAGCTG 2 cut(s) 155, 1397
RsaI GTAC 2 cut(s) 904, 967
RsaNI GTAC 2 cut(s) 903, 966
RseI CAYNNNNRTG 3 cut(s) 1010, 1232, 1257
SacI GAGCTC 2 cut(s) 531, 1119
SalI GTCGAC 1 cut(s) 1688
SatI GCNGC 9 cut(s) 153, 479, 546, 1372, 1642, 1684, 1705, 1723, 1833
Sau3AI GATC 6 cut(s) 67, 456, 1019, 1314, 1402, 1716
Sau96I GGNCC 6 cut(s) 337, 956, 1575, 1599, 1780, 1786
ScaI AGTACT 1 cut(s) 904
SchI GAGTC 2 cut(s) 1237, 1577
ScrFI CCNGG 3 cut(s) 1322, 1597, 1784
SduI GDGCHC 2 cut(s) 531, 1119
SfaNI GCATC 3 cut(s) 333, 1401, 1446
SfcI CTRYAG 3 cut(s) 233, 513, 1814
SinI GGWCC 4 cut(s) 337, 1599, 1780, 1786
SmiMI CAYNNNNRTG 3 cut(s) 1010, 1232, 1257
SmlI CTYRAG 1 cut(s) 1415
SmoI CTYRAG 1 cut(s) 1415
SpeI ACTAGT 1 cut(s) 1291
SsiI CCGC 6 cut(s) 1325, 1369, 1702, 1720, 1723, 1858
SspI AATATT 1 cut(s) 186
SstI GAGCTC 2 cut(s) 531, 1119
StyD4I CCNGG 3 cut(s) 1320, 1595, 1782
StyI CCWWGG 2 cut(s) 1336, 1789
TaaI ACNGT 5 cut(s) 20, 55, 311, 816, 944
TaiI ACGT 3 cut(s) 477, 931, 1779
TaqI TCGA 3 cut(s) 1018, 1689, 1867
TatI WGTACW 1 cut(s) 902
TauI GCSGC 1 cut(s) 1725
TfiI GAWTC 3 cut(s) 775, 1561, 1819
TscAI CASTG 6 cut(s) 25, 58, 819, 927, 1035, 1081
TseFI GTSAC 2 cut(s) 49, 1353
TseI GCWGC 8 cut(s) 152, 478, 545, 1371, 1641, 1683, 1704, 1832
Tsp45I GTSAC 2 cut(s) 49, 1353
TspDTI ATGAA 8 cut(s) 108, 875, 903, 960, 1094, 1160, 1639, 1853
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 6 cut(s) 25, 58, 819, 927, 1035, 1081
Tth111I GACNNNGTC 2 cut(s) 1694, 1778
Vha464I CTTAAG 1 cut(s) 1415
VpaK11BI GGWCC 4 cut(s) 337, 1599, 1780, 1786
XapI RAATTY 6 cut(s) 192, 300, 349, 882, 1100, 1465
XcmI CCANNNNNNNNNTGG 1 cut(s) 1328
XmiI GTMKAC 2 cut(s) 855, 1689
ZrmI AGTACT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.