FvH4_2g19730

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000965
Physical Location & Seq
Forward (+)
16669596 .. 16670456
861 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19730.t1

Sequence Viewer

Length: 441 bp
ATGATAGCTCTCAAAGGCATTGCCATTTTCTACTTGTGTGCAACTTTGGCACTTAGCATCTGCCCGGAATCCTATGCCAAATGGCCCGGTCTTGATCCCACCAATTGGAATGTGCACGTTGTTAACGGTTTGAGTGGAGGAAGACAACTGGTTGTCCACTGCAAGTCCGACGTCGACGATCTCGGCATCCATACTCTTGGTCCTGGTGGCGACTTCAATTGGCATTTCCATGTCAACTTGCCTGGGACAACAAAATTCACTTGCGACTGGCGTGCCGGCAATCTACATGCTGTGACTGATGTGTTCTGGAGGGAGGCTGAACATATGTGGCTTCGGACCAAATGCAACTGGAAAGAGTGTTTTTGGACTGCGAAAGACGATGGCATTTACCTCAAAGACATTCCGGCGAATAGTGAAGAGCGTGTTCATCCATGGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

147

Amino Acids

16.62

Weight (kDa)

6.03

Isoelectric Point (pI)

22.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 37 - 145 3.1e-25 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 174
AccB7I CCANNNNNTGG 1 cut(s) 105
AccI GTMKAC 1 cut(s) 174
AclWI GGATC 1 cut(s) 89
AcsI RAATTY 1 cut(s) 254
AcyI GRCGYC 1 cut(s) 171
AfiI CCNNNNNNNGG 1 cut(s) 105
AgsI TTSAA 1 cut(s) 217
AjnI CCWGG 2 cut(s) 202, 241
AluBI AGCT 1 cut(s) 8
AluI AGCT 1 cut(s) 8
Alw21I GWGCWC 1 cut(s) 117
Alw44I GTGCAC 1 cut(s) 113
AlwI GGATC 1 cut(s) 89
AoxI GGCC 1 cut(s) 83
ApaLI GTGCAC 1 cut(s) 113
ApoI RAATTY 1 cut(s) 254
AspS9I GGNCC 3 cut(s) 84, 200, 336
AsuC2I CCSGG 2 cut(s) 65, 87
AvaII GGWCC 2 cut(s) 200, 336
BaeGI GKGCMC 1 cut(s) 117
BbsI GAAGAC 1 cut(s) 148
Bbv12I GWGCWC 1 cut(s) 117
BccI CCATC 1 cut(s) 374
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 2 cut(s) 204, 243
BcnI CCSGG 2 cut(s) 65, 87
Bme1390I CCNGG 4 cut(s) 65, 87, 204, 243
Bme18I GGWCC 2 cut(s) 200, 336
BmgT120I GGNCC 3 cut(s) 84, 200, 336
BmrFI CCNGG 4 cut(s) 65, 87, 204, 243
BmsI GCATC 2 cut(s) 66, 195
BpiI GAAGAC 1 cut(s) 148
BpmI CTGGAG 1 cut(s) 328
BpuMI CCSGG 2 cut(s) 65, 87
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 2 cut(s) 242, 431
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 3 cut(s) 153, 272, 353
Bse3DI GCAATG 1 cut(s) 18
BseBI CCWGG 2 cut(s) 204, 243
BseDI CCNNGG 2 cut(s) 242, 431
BseGI GGATG 2 cut(s) 186, 427
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 3 cut(s) 153, 272, 353
BseSI GKGCMC 1 cut(s) 117
BshFI GGCC 1 cut(s) 85
BsiHKAI GWGCWC 1 cut(s) 117
BsiSI CCGG 4 cut(s) 65, 87, 276, 404
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 259
BsnI GGCC 1 cut(s) 85
Bsp1286I GDGCHC 1 cut(s) 117
Bsp143I GATC 2 cut(s) 94, 178
Bsp19I CCATGG 1 cut(s) 431
BspANI GGCC 1 cut(s) 85
BspPI GGATC 1 cut(s) 89
BspQI GCTCTTC 1 cut(s) 411
BsrDI GCAATG 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 3 cut(s) 153, 272, 353
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 242, 431
BssMI GATC 2 cut(s) 94, 178
BssNI GRCGYC 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 431
Bst2UI CCWGG 2 cut(s) 204, 243
Bst4CI ACNGT 1 cut(s) 128
Bst6I CTCTTC 1 cut(s) 411
BstACI GRCGYC 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 273, 277
BstDEI CTNAG 1 cut(s) 53
BstDSI CCRYGG 1 cut(s) 431
BstF5I GGATG 2 cut(s) 186, 427
BstKTI GATC 2 cut(s) 97, 181
BstMBI GATC 2 cut(s) 94, 178
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 2 cut(s) 204, 243
BstNSI RCATGY 1 cut(s) 290
BstSCI CCNGG 4 cut(s) 63, 85, 202, 241
BstSLI GKGCMC 1 cut(s) 117
BstV2I GAAGAC 1 cut(s) 148
BstXI CCANNNNNNTGG 1 cut(s) 197
BsuRI GGCC 1 cut(s) 85
BtgI CCRYGG 1 cut(s) 431
BtsCI GGATG 2 cut(s) 186, 427
BtsI GCAGTG 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 157
Cac8I GCNNGC 2 cut(s) 273, 277
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 3 cut(s) 84, 200, 336
CviAII CATG 3 cut(s) 230, 287, 432
CviJI RGCY 4 cut(s) 8, 85, 317, 331
CviKI_1 RGCY 4 cut(s) 8, 85, 317, 331
DdeI CTNAG 1 cut(s) 53
DpnI GATC 2 cut(s) 96, 180
DpnII GATC 2 cut(s) 94, 178
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Eco130I CCWWGG 1 cut(s) 431
Eco47I GGWCC 2 cut(s) 200, 336
EcoRII CCWGG 2 cut(s) 202, 241
EcoT14I CCWWGG 1 cut(s) 431
ErhI CCWWGG 1 cut(s) 431
FaeI CATG 3 cut(s) 233, 290, 435
FaiI YATR 7 cut(s) 75, 192, 231, 288, 324, 326, 433
FaqI GGGAC 1 cut(s) 259
FatI CATG 3 cut(s) 229, 286, 431
FauNDI CATATG 1 cut(s) 324
FblI GTMKAC 1 cut(s) 174
FokI GGATG 2 cut(s) 173, 414
GsuI CTGGAG 1 cut(s) 328
HaeIII GGCC 1 cut(s) 85
HapII CCGG 4 cut(s) 65, 87, 276, 404
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 3 cut(s) 233, 290, 435
HincII GTYRAC 3 cut(s) 124, 175, 235
HindII GTYRAC 3 cut(s) 124, 175, 235
HinfI GANTC 1 cut(s) 68
HpaI GTTAAC 1 cut(s) 124
HpaII CCGG 4 cut(s) 65, 87, 276, 404
Hpy166II GTNNAC 5 cut(s) 115, 124, 157, 175, 235
Hpy188I TCNGA 2 cut(s) 169, 336
Hpy188III TCNNGA 2 cut(s) 92, 307
Hpy8I GTNNAC 5 cut(s) 115, 124, 157, 175, 235
Hpy99I CGWCG 3 cut(s) 173, 176, 179
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4IV ACGT 2 cut(s) 117, 171
HpyCH4V TGCA 4 cut(s) 41, 115, 162, 345
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 2 cut(s) 117, 171
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 3 cut(s) 233, 290, 435
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
KspAI GTTAAC 1 cut(s) 124
Kzo9I GATC 2 cut(s) 94, 178
LguI GCTCTTC 1 cut(s) 411
LweI GCATC 2 cut(s) 66, 195
MaeII ACGT 2 cut(s) 117, 171
MaeIII GTNAC 1 cut(s) 292
MalI GATC 2 cut(s) 96, 180
MboI GATC 2 cut(s) 94, 178
MboII GAAGA 2 cut(s) 153, 428
MfeI CAATTG 2 cut(s) 103, 217
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 3 cut(s) 103, 217, 254
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 4 cut(s) 131, 303, 307, 401
MroNI GCCGGC 1 cut(s) 275
MseI TTAA 1 cut(s) 123
MslI CAYNNNNRTG 1 cut(s) 228
MspI CCGG 4 cut(s) 65, 87, 276, 404
MspR9I CCNGG 4 cut(s) 65, 87, 204, 243
MunI CAATTG 2 cut(s) 103, 217
MvaI CCWGG 2 cut(s) 204, 243
MwoI GCNNNNNNNGC 1 cut(s) 47
NaeI GCCGGC 1 cut(s) 277
NciI CCSGG 2 cut(s) 65, 87
NcoI CCATGG 1 cut(s) 431
NdeI CATATG 1 cut(s) 324
NdeII GATC 2 cut(s) 94, 178
NgoMIV GCCGGC 1 cut(s) 275
NlaIII CATG 3 cut(s) 233, 290, 435
NmeAIII GCCGAG 1 cut(s) 162
NmuCI GTSAC 1 cut(s) 292
NspI RCATGY 1 cut(s) 290
PciSI GCTCTTC 1 cut(s) 411
PdiI GCCGGC 1 cut(s) 277
PfeI GAWTC 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 105
Psp6I CCWGG 2 cut(s) 202, 241
PspGI CCWGG 2 cut(s) 202, 241
PspPI GGNCC 3 cut(s) 84, 200, 336
RseI CAYNNNNRTG 1 cut(s) 228
SalI GTCGAC 1 cut(s) 173
SapI GCTCTTC 1 cut(s) 411
SaqAI TTAA 1 cut(s) 123
Sau3AI GATC 2 cut(s) 94, 178
Sau96I GGNCC 3 cut(s) 84, 200, 336
ScrFI CCNGG 4 cut(s) 65, 87, 204, 243
SduI GDGCHC 1 cut(s) 117
SetI ASST 4 cut(s) 10, 120, 174, 393
SfaNI GCATC 2 cut(s) 66, 195
SgrDI CGTCGACG 1 cut(s) 173
SinI GGWCC 2 cut(s) 200, 336
SmiMI CAYNNNNRTG 1 cut(s) 228
Sse9I AATT 3 cut(s) 103, 217, 254
StyD4I CCNGG 4 cut(s) 63, 85, 202, 241
StyI CCWWGG 1 cut(s) 431
TaaI ACNGT 1 cut(s) 128
TaiI ACGT 2 cut(s) 120, 174
TaqI TCGA 1 cut(s) 174
TasI AATT 3 cut(s) 103, 217, 254
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TscAI CASTG 1 cut(s) 164
TseFI GTSAC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 1 cut(s) 416
TspRI CASTG 1 cut(s) 164
Van91I CCANNNNNTGG 1 cut(s) 105
VneI GTGCAC 1 cut(s) 113
VpaK11BI GGWCC 2 cut(s) 200, 336
XapI RAATTY 1 cut(s) 254
XceI RCATGY 1 cut(s) 290
XmiI GTMKAC 1 cut(s) 174
ZraI GACGTC 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.