Rroxscaffold_1G00040500

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000965
Physical Location & Seq
Forward (+)
58225435 .. 58229996
4562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00040500.1

Sequence Viewer

Length: 525 bp
ATGAGTGGTTTCAAGCTCTATGTGGTTGCGCTTGTGTCTTTCACATTCTTCGCATTGACTATCCCATGTTCTGCTGTGACGTGGCATGTTCACGTCGTTAATGGATTGAGCTCTGGAAGAATTCTCTTCGTCCATTGCAAGTCTAAAGATAATGATCTCGGCATCCATAATCTTGCAGTCGGAACCGAAACCAACTGGAGTTTCAAAGAAAACTTTACAGGGACAACACTTTTTTGGTGCTACCTGCGCACGTACCGTGAAGAGTATGCCGATTTTGATGTGTTCTGGTCGGAGCATAATCGTAAGTGGCTTCGGACTAGATGCAACTGGAAAGATTGCATATGGACCGCAAGAGATGACGGGGTTTACATAAAGAACATTCCTAACAACGGGCGTGACAGAGGCCCAACCACTCCTAGGTCCCAAGCCCAAAACAGAAAACCAAACCAAAGCCCTACCCCAATTAGGGTTTGCCATTGCAGCCAACCCAACAGAGCCATCAACTTTGCCGCCACCGACACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

174

Amino Acids

20.08

Weight (kDa)

9.19

Isoelectric Point (pI)

25.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 29 - 126 1.3e-28 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 248
Acc36I ACCTGC 1 cut(s) 252
AciI CCGC 2 cut(s) 348, 510
AcsI RAATTY 1 cut(s) 120
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 4 cut(s) 389, 417, 465, 466
AgsI TTSAA 2 cut(s) 13, 205
AjiI CACGTC 2 cut(s) 81, 94
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AluBI AGCT 2 cut(s) 16, 111
AluI AGCT 2 cut(s) 16, 111
Alw21I GWGCWC 1 cut(s) 113
AoxI GGCC 1 cut(s) 403
ApeKI GCWGC 1 cut(s) 480
ApoI RAATTY 1 cut(s) 120
AspA2I CCTAGG 1 cut(s) 416
AspLEI GCGC 2 cut(s) 31, 249
AspS9I GGNCC 3 cut(s) 345, 404, 420
AvaII GGWCC 2 cut(s) 345, 420
AvrII CCTAGG 1 cut(s) 416
BanII GRGCYC 1 cut(s) 113
Bbv12I GWGCWC 1 cut(s) 113
BbvI GCAGC 1 cut(s) 492
BccI CCATC 1 cut(s) 506
BfaI CTAG 2 cut(s) 318, 417
BfuAI ACCTGC 1 cut(s) 252
BisI GCNGC 2 cut(s) 481, 510
BlnI CCTAGG 1 cut(s) 416
BlsI GCNGC 2 cut(s) 482, 511
Bme18I GGWCC 2 cut(s) 345, 420
BmgBI CACGTC 2 cut(s) 81, 94
BmgT120I GGNCC 3 cut(s) 345, 404, 420
BmiI GGNNCC 2 cut(s) 184, 422
BmsI GCATC 2 cut(s) 171, 311
BpmI CTGGAG 1 cut(s) 217
BsaAI YACGTR 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 416
Bsc4I CCNNNNNNNGG 4 cut(s) 389, 417, 465, 466
Bse1I ACTGG 2 cut(s) 200, 332
Bse3DI GCAATG 2 cut(s) 133, 475
Bse8I GATNNNNATC 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 416
BseGI GGATG 1 cut(s) 162
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 4 cut(s) 389, 417, 465, 466
BseMI GCAATG 2 cut(s) 133, 475
BseNI ACTGG 2 cut(s) 200, 332
BseXI GCAGC 1 cut(s) 492
BshFI GGCC 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 113
BslFI GGGAC 2 cut(s) 235, 406
BslI CCNNNNNNNGG 4 cut(s) 389, 417, 465, 466
BsmFI GGGAC 2 cut(s) 235, 406
BsnI GGCC 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 113
Bsp143I GATC 1 cut(s) 154
BspACI CCGC 2 cut(s) 348, 510
BspANI GGCC 1 cut(s) 405
BspLI GGNNCC 2 cut(s) 184, 422
BspMI ACCTGC 1 cut(s) 252
BsrDI GCAATG 2 cut(s) 133, 475
BsrI ACTGG 2 cut(s) 200, 332
BssECI CCNNGG 1 cut(s) 416
BssMI GATC 1 cut(s) 154
BssT1I CCWWGG 1 cut(s) 416
Bst4CI ACNGT 1 cut(s) 257
Bst6I CTCTTC 2 cut(s) 131, 255
BstBAI YACGTR 1 cut(s) 252
BstF5I GGATG 1 cut(s) 162
BstHHI GCGC 2 cut(s) 31, 249
BstKTI GATC 1 cut(s) 157
BstMBI GATC 1 cut(s) 154
BstMWI GCNNNNNNNGC 2 cut(s) 246, 480
BstNSI RCATGY 1 cut(s) 89
BstV1I GCAGC 1 cut(s) 492
BsuRI GGCC 1 cut(s) 405
BtrI CACGTC 2 cut(s) 81, 94
BtsCI GGATG 1 cut(s) 162
BveI ACCTGC 1 cut(s) 252
CfoI GCGC 2 cut(s) 31, 249
Cfr13I GGNCC 3 cut(s) 345, 404, 420
Csp6I GTAC 1 cut(s) 253
CviAII CATG 2 cut(s) 66, 86
CviJI RGCY 8 cut(s) 16, 111, 310, 405, 428, 453, 483, 497
CviKI_1 RGCY 8 cut(s) 16, 111, 310, 405, 428, 453, 483, 497
CviQI GTAC 1 cut(s) 253
DpnI GATC 1 cut(s) 156
DpnII GATC 1 cut(s) 154
Eam1104I CTCTTC 2 cut(s) 131, 255
EarI CTCTTC 2 cut(s) 131, 255
Ecl136II GAGCTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 416
Eco24I GRGCYC 1 cut(s) 113
Eco47I GGWCC 2 cut(s) 345, 420
Eco53kI GAGCTC 1 cut(s) 111
EcoICRI GAGCTC 1 cut(s) 111
EcoO109I RGGNCCY 1 cut(s) 420
EcoRI GAATTC 1 cut(s) 120
EcoT14I CCWWGG 1 cut(s) 416
EcoT38I GRGCYC 1 cut(s) 113
ErhI CCWWGG 1 cut(s) 416
FaeI CATG 2 cut(s) 69, 89
FaiI YATR 9 cut(s) 21, 67, 87, 168, 267, 297, 341, 343, 371
FaqI GGGAC 2 cut(s) 235, 406
FatI CATG 2 cut(s) 65, 85
FauNDI CATATG 1 cut(s) 341
Fnu4HI GCNGC 2 cut(s) 481, 510
FokI GGATG 1 cut(s) 149
FriOI GRGCYC 1 cut(s) 113
Fsp4HI GCNGC 2 cut(s) 481, 510
FspBI CTAG 2 cut(s) 318, 417
FspI TGCGCA 1 cut(s) 248
GlaI GCGC 2 cut(s) 30, 248
GluI GCNGC 2 cut(s) 481, 510
GsuI CTGGAG 1 cut(s) 217
HaeIII GGCC 1 cut(s) 405
HhaI GCGC 2 cut(s) 31, 249
Hin1II CATG 2 cut(s) 69, 89
Hin6I GCGC 2 cut(s) 29, 247
HinP1I GCGC 2 cut(s) 29, 247
Hpy166II GTNNAC 2 cut(s) 91, 367
Hpy188I TCNGA 3 cut(s) 182, 292, 315
Hpy188III TCNNGA 1 cut(s) 114
Hpy8I GTNNAC 2 cut(s) 91, 367
Hpy99I CGWCG 1 cut(s) 98
HpyCH4III ACNGT 1 cut(s) 257
HpyCH4IV ACGT 3 cut(s) 80, 93, 251
HpyCH4V TGCA 5 cut(s) 138, 176, 324, 339, 480
HpyF10VI GCNNNNNNNGC 2 cut(s) 246, 480
HpySE526I ACGT 3 cut(s) 80, 93, 251
Hsp92II CATG 2 cut(s) 69, 89
HspAI GCGC 2 cut(s) 29, 247
Kzo9I GATC 1 cut(s) 154
LmnI GCTCC 1 cut(s) 292
LpnPI CCDG 6 cut(s) 99, 181, 204, 257, 271, 313
Lsp1109I GCAGC 1 cut(s) 492
LweI GCATC 2 cut(s) 171, 311
MaeI CTAG 2 cut(s) 318, 417
MaeII ACGT 3 cut(s) 80, 93, 251
MaeIII GTNAC 2 cut(s) 76, 395
MalI GATC 1 cut(s) 156
MboI GATC 1 cut(s) 154
MboII GAAGA 4 cut(s) 40, 118, 129, 272
MhlI GDGCHC 1 cut(s) 113
MluCI AATT 2 cut(s) 120, 462
MmeI TCCRAC 2 cut(s) 160, 270
MnlI CCTC 1 cut(s) 395
MseI TTAA 1 cut(s) 99
MwoI GCNNNNNNNGC 2 cut(s) 246, 480
NdeI CATATG 1 cut(s) 341
NdeII GATC 1 cut(s) 154
NlaIII CATG 2 cut(s) 69, 89
NlaIV GGNNCC 2 cut(s) 184, 422
NmeAIII GCCGAG 1 cut(s) 138
NmuCI GTSAC 2 cut(s) 76, 395
NsbI TGCGCA 1 cut(s) 248
NspI RCATGY 1 cut(s) 89
PkrI GCNGC 2 cut(s) 482, 511
Ppu21I YACGTR 1 cut(s) 252
PpuMI RGGWCCY 1 cut(s) 420
Psp124BI GAGCTC 1 cut(s) 113
Psp5II RGGWCCY 1 cut(s) 420
PspN4I GGNNCC 2 cut(s) 184, 422
PspPI GGNCC 3 cut(s) 345, 404, 420
PspPPI RGGWCCY 1 cut(s) 420
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
SacI GAGCTC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 2 cut(s) 481, 510
Sau3AI GATC 1 cut(s) 154
Sau96I GGNCC 3 cut(s) 345, 404, 420
SduI GDGCHC 1 cut(s) 113
SetI ASST 8 cut(s) 18, 83, 96, 113, 246, 254, 422, 524
SfaNI GCATC 2 cut(s) 171, 311
SinI GGWCC 2 cut(s) 345, 420
Sse9I AATT 2 cut(s) 120, 462
SsiI CCGC 2 cut(s) 348, 510
SspMI CTAG 2 cut(s) 318, 417
SstI GAGCTC 1 cut(s) 113
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 1 cut(s) 257
TaiI ACGT 3 cut(s) 83, 96, 254
TasI AATT 2 cut(s) 120, 462
TauI GCSGC 1 cut(s) 512
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TseFI GTSAC 2 cut(s) 76, 395
TseI GCWGC 1 cut(s) 480
Tsp45I GTSAC 2 cut(s) 76, 395
VpaK11BI GGWCC 2 cut(s) 345, 420
XapI RAATTY 1 cut(s) 120
XceI RCATGY 1 cut(s) 89
XmaJI CCTAGG 1 cut(s) 416
XspI CTAG 2 cut(s) 318, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.