Rorug05G0178100

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000965
Physical Location & Seq
Reverse (-)
16646922 .. 16650132
3211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0178100.1

Sequence Viewer

Length: 1143 bp
ATGAAATCTAGATTTGATTCTGTATTCAAGCTCTGCATAGTGTTCCCTGCCGGTCGAAATCACTCTCCTTTCCCCAGAACTGTTCTCCCAACTTGTTTAAGTTTCGTGCACACCACCTATTCGACAGTTTGCCTCACTCAGGAAGAAAGTGTAGTGGAAGACATTTTTGACCAACCAAAGACCTCTGCAGAAGTTCTGGAGAAATGGGGTTGTAGTGATGCTGACATATCGAAGCTGTTCAGGCGCCGGCCTACTTTAAGGAATGCTGACCTTAACCAGCTTCAGTTCAAGTTAAACCTTTTGAGTGGATTGGGTATTACTGCACCTGAGCTTTTGAAGATGATCAATTGTCGCCCCCGGTTGCTCAGTGTTCGGATTGATCATTGCTTTGATGAGCGGCATGAGTTCTTTATGAAATTGTTTGGGTCAAGGGAACTGCTTGTCAAAGCCATTGTAAGGAACCCGTCTCTCTTGATCTATGACTTCCATAATAAAGTCAAGCCGACCATTGCTCTATATGAGGAAATGGGTCTTAGTCGGGAGGAGTTAGTCCCAATGCTAATATCACGGCCGACATTGATCCCAAGAACTACATTTAACGACGAGAAGATGGAGTATATACGGAAAACTGGACTGTCAAATGAGTCCAGGATGTATAAGTATGTTGTGACACTCATTGGCATTTCACGCCTTGAAACTATCAGTGAGAAAGTGGCAAATTTTGAAAAGTTTGGTTTCTCAGAAGATGAGATTTTCGGCCTTTTGGGAAGGTCTCCCATAGTGTTTACTCTTTCAGTGGATAAGGTCCAGAGGAACATGACTTTCATTTTAGGACAAATGAAACTTCCTGCTACAGTGATTCTTGAGCACCCATTTTTGCTGTACATGAATCTGGAGGCTGCTTTGAAGCCGCGAGTATGTCTTGCAGGAAAAATTGAGGATATGGATCTTGATCTGCAAATTAAAGGACCTAAGTTGCTGACGGCATTGAGAATGACAGAGAAAAGATTTGTGAAGGCATTTGTTAATTGTCACCCAAAGGATGTTGCTGATGAATTGATGGAGCACTATACAAAAGCAAAGGGTGTGAAGCGATTGGCAGAAGCCTCAAAGAAGAAATTGTATGAAGGCTTTCCTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

380

Amino Acids

43.72

Weight (kDa)

9.1

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTERF PF02536 64 - 138 5.9e-12 mTERF
mTERF PF02536 135 - 337 2.2e-32 mTERF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 243
AccBSI CCGCTC 1 cut(s) 397
AccII CGCG 1 cut(s) 913
AciI CCGC 2 cut(s) 397, 911
AclWI GGATC 2 cut(s) 574, 954
AcoI YGGCCR 1 cut(s) 569
AcsI RAATTY 1 cut(s) 718
AcuI CTGAAG 1 cut(s) 266
AcyI GRCGYC 1 cut(s) 244
AfaI GTAC 1 cut(s) 884
AfiI CCNNNNNNNGG 2 cut(s) 139, 456
AgsI TTSAA 6 cut(s) 28, 289, 337, 695, 725, 907
AjnI CCWGG 1 cut(s) 647
AluBI AGCT 4 cut(s) 31, 235, 280, 331
AluI AGCT 4 cut(s) 31, 235, 280, 331
Alw21I GWGCWC 3 cut(s) 111, 870, 1068
Alw26I GTCTC 2 cut(s) 471, 777
Alw44I GTGCAC 1 cut(s) 107
AlwI GGATC 2 cut(s) 574, 954
AoxI GGCC 3 cut(s) 248, 569, 757
ApaLI GTGCAC 1 cut(s) 107
ApeKI GCWGC 1 cut(s) 899
ApoI RAATTY 1 cut(s) 718
Asp700I GAANNNNTTC 2 cut(s) 236, 1131
AspLEI GCGC 1 cut(s) 246
AspS9I GGNCC 2 cut(s) 805, 968
AsuC2I CCSGG 1 cut(s) 358
AsuHPI GGTGA 1 cut(s) 1025
AvaII GGWCC 2 cut(s) 805, 968
BaeGI GKGCMC 1 cut(s) 111
BanI GGYRCC 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 165
Bbv12I GWGCWC 3 cut(s) 111, 870, 1068
BbvI GCAGC 1 cut(s) 886
BccI CCATC 2 cut(s) 604, 1054
BceAI ACGGC 2 cut(s) 584, 999
BciT130I CCWGG 1 cut(s) 649
BclI TGATCA 2 cut(s) 342, 379
BcnI CCSGG 1 cut(s) 358
BcoDI GTCTC 2 cut(s) 471, 777
BfaI CTAG 1 cut(s) 9
BfmI CTRYAG 2 cut(s) 186, 852
BfoI RGCGCY 1 cut(s) 247
BisI GCNGC 3 cut(s) 398, 900, 911
BlsI GCNGC 3 cut(s) 399, 901, 912
Bme1390I CCNGG 2 cut(s) 358, 649
Bme18I GGWCC 2 cut(s) 805, 968
BmgT120I GGNCC 2 cut(s) 805, 968
BmiI GGNNCC 2 cut(s) 245, 461
BmrFI CCNGG 2 cut(s) 358, 649
BmsI GCATC 1 cut(s) 208
BpiI GAAGAC 1 cut(s) 165
BpmI CTGGAG 2 cut(s) 218, 914
Bpu10I CCTNAGC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 884
BpuMI CCSGG 1 cut(s) 358
BsaBI GATNNNNATC 2 cut(s) 945, 951
BsaHI GRCGYC 1 cut(s) 244
BsaI GGTCTC 1 cut(s) 777
BsaJI CCNNGG 1 cut(s) 356
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 456
Bse118I RCCGGY 2 cut(s) 50, 246
Bse1I ACTGG 1 cut(s) 634
Bse3DI GCAATG 2 cut(s) 382, 507
Bse8I GATNNNNATC 2 cut(s) 945, 951
BseBI CCWGG 1 cut(s) 649
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 2 cut(s) 657, 1048
BseJI GATNNNNATC 2 cut(s) 945, 951
BseLI CCNNNNNNNGG 2 cut(s) 139, 456
BseMI GCAATG 2 cut(s) 382, 507
BseMII CTCAG 4 cut(s) 152, 318, 379, 753
BseNI ACTGG 1 cut(s) 634
BseRI GAGGAG 1 cut(s) 557
BseSI GKGCMC 1 cut(s) 111
BseX3I CGGCCG 1 cut(s) 569
BseXI GCAGC 1 cut(s) 886
BsgI GTGCAG 1 cut(s) 306
Bsh1236I CGCG 1 cut(s) 913
Bsh1285I CGRYCG 2 cut(s) 55, 572
BshFI GGCC 3 cut(s) 250, 571, 759
BshNI GGYRCC 1 cut(s) 243
BsiEI CGRYCG 2 cut(s) 55, 572
BsiHKAI GWGCWC 3 cut(s) 111, 870, 1068
BsiSI CCGG 3 cut(s) 51, 247, 358
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 2 cut(s) 139, 456
BsmAI GTCTC 2 cut(s) 471, 777
BsmBI CGTCTC 1 cut(s) 471
BsmFI GGGAC 1 cut(s) 536
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 3 cut(s) 250, 571, 759
Bso31I GGTCTC 1 cut(s) 777
Bsp1286I GDGCHC 3 cut(s) 111, 870, 1068
Bsp1407I TGTACA 1 cut(s) 882
Bsp143I GATC 6 cut(s) 342, 379, 474, 579, 946, 952
BspACI CCGC 2 cut(s) 397, 911
BspANI GGCC 3 cut(s) 250, 571, 759
BspCNI CTCAG 4 cut(s) 151, 319, 378, 752
BspFNI CGCG 1 cut(s) 913
BspLI GGNNCC 2 cut(s) 245, 461
BspMAI CTGCAG 1 cut(s) 190
BspPI GGATC 2 cut(s) 574, 954
BspT107I GGYRCC 1 cut(s) 243
BspTNI GGTCTC 1 cut(s) 777
BsrBI CCGCTC 1 cut(s) 397
BsrDI GCAATG 2 cut(s) 382, 507
BsrFI RCCGGY 2 cut(s) 50, 246
BsrGI TGTACA 1 cut(s) 882
BsrI ACTGG 1 cut(s) 634
BssAI RCCGGY 2 cut(s) 50, 246
BssECI CCNNGG 1 cut(s) 356
BssMI GATC 6 cut(s) 342, 379, 474, 579, 946, 952
BssNI GRCGYC 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 649
Bst4CI ACNGT 4 cut(s) 82, 127, 636, 856
BstACI GRCGYC 1 cut(s) 244
BstAUI TGTACA 1 cut(s) 882
BstC8I GCNNGC 1 cut(s) 248
BstDEI CTNAG 6 cut(s) 138, 327, 365, 533, 739, 972
BstENI CCTNNNNNAGG 1 cut(s) 137
BstF5I GGATG 2 cut(s) 657, 1048
BstFNI CGCG 1 cut(s) 913
BstH2I RGCGCY 1 cut(s) 247
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 6 cut(s) 345, 382, 477, 582, 949, 955
BstMAI GTCTC 2 cut(s) 471, 777
BstMBI GATC 6 cut(s) 342, 379, 474, 579, 946, 952
BstMCI CGRYCG 2 cut(s) 55, 572
BstMWI GCNNNNNNNGC 2 cut(s) 241, 687
BstNI CCWGG 1 cut(s) 649
BstSCI CCNGG 2 cut(s) 356, 647
BstSFI CTRYAG 2 cut(s) 186, 852
BstSLI GKGCMC 1 cut(s) 111
BstUI CGCG 1 cut(s) 913
BstV1I GCAGC 1 cut(s) 886
BstV2I GAAGAC 1 cut(s) 165
BstX2I RGATCY 1 cut(s) 946
BstYI RGATCY 1 cut(s) 946
BstZI CGGCCG 1 cut(s) 569
BsuRI GGCC 3 cut(s) 250, 571, 759
BtsCI GGATG 2 cut(s) 657, 1048
BtsIMutI CAGTG 4 cut(s) 373, 709, 801, 861
Cac8I GCNNGC 1 cut(s) 248
CfoI GCGC 1 cut(s) 246
Cfr10I RCCGGY 2 cut(s) 50, 246
Cfr13I GGNCC 2 cut(s) 805, 968
Csp6I GTAC 1 cut(s) 883
CviAII CATG 3 cut(s) 401, 817, 886
CviQI GTAC 1 cut(s) 883
DdeI CTNAG 6 cut(s) 138, 327, 365, 533, 739, 972
DinI GGCGCC 1 cut(s) 245
DpnI GATC 6 cut(s) 344, 381, 476, 581, 948, 954
DpnII GATC 6 cut(s) 342, 379, 474, 579, 946, 952
EaeI YGGCCR 1 cut(s) 569
EagI CGGCCG 1 cut(s) 569
EclXI CGGCCG 1 cut(s) 569
Eco31I GGTCTC 1 cut(s) 777
Eco47I GGWCC 2 cut(s) 805, 968
Eco52I CGGCCG 1 cut(s) 569
Eco57I CTGAAG 1 cut(s) 266
EcoNI CCTNNNNNAGG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 968
EcoRII CCWGG 1 cut(s) 647
EgeI GGCGCC 1 cut(s) 245
EheI GGCGCC 1 cut(s) 245
Esp3I CGTCTC 1 cut(s) 471
FaeI CATG 3 cut(s) 404, 820, 889
FaqI GGGAC 1 cut(s) 536
FatI CATG 3 cut(s) 400, 816, 885
FbaI TGATCA 2 cut(s) 342, 379
Fnu4HI GCNGC 3 cut(s) 398, 900, 911
FokI GGATG 2 cut(s) 664, 1055
Fsp4HI GCNGC 3 cut(s) 398, 900, 911
FspBI CTAG 1 cut(s) 9
GlaI GCGC 1 cut(s) 245
GluI GCNGC 3 cut(s) 398, 900, 911
GsuI CTGGAG 2 cut(s) 218, 914
HaeII RGCGCY 1 cut(s) 247
HaeIII GGCC 3 cut(s) 250, 571, 759
HapII CCGG 3 cut(s) 51, 247, 358
HhaI GCGC 1 cut(s) 246
Hin1I GRCGYC 1 cut(s) 244
Hin1II CATG 3 cut(s) 404, 820, 889
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HinfI GANTC 4 cut(s) 17, 644, 859, 889
HpaII CCGG 3 cut(s) 51, 247, 358
HphI GGTGA 1 cut(s) 1025
Hpy166II GTNNAC 2 cut(s) 109, 786
Hpy188I TCNGA 2 cut(s) 375, 742
Hpy188III TCNNGA 9 cut(s) 9, 140, 197, 472, 539, 808, 863, 893, 950
Hpy8I GTNNAC 2 cut(s) 109, 786
Hpy99I CGWCG 1 cut(s) 605
HpyAV CCTTC 3 cut(s) 762, 1009, 1121
HpyCH4III ACNGT 4 cut(s) 82, 127, 636, 856
HpyCH4V TGCA 6 cut(s) 36, 109, 188, 323, 926, 958
HpyF10VI GCNNNNNNNGC 2 cut(s) 241, 687
HpyF3I CTNAG 6 cut(s) 138, 327, 365, 533, 739, 972
Hsp92I GRCGYC 1 cut(s) 244
Hsp92II CATG 3 cut(s) 404, 820, 889
HspAI GCGC 1 cut(s) 244
KasI GGCGCC 1 cut(s) 243
KroI GCCGGC 1 cut(s) 246
KroNI GCCGGC 1 cut(s) 248
Ksp22I TGATCA 2 cut(s) 342, 379
Kzo9I GATC 6 cut(s) 342, 379, 474, 579, 946, 952
LmnI GCTCC 1 cut(s) 1063
Lsp1109I GCAGC 1 cut(s) 886
LweI GCATC 1 cut(s) 208
MaeI CTAG 1 cut(s) 9
MaeIII GTNAC 2 cut(s) 667, 1031
MalI GATC 6 cut(s) 344, 381, 476, 581, 948, 954
MbiI CCGCTC 1 cut(s) 397
MboI GATC 6 cut(s) 342, 379, 474, 579, 946, 952
MboII GAAGA 6 cut(s) 155, 170, 349, 619, 755, 1126
MfeI CAATTG 1 cut(s) 346
MflI RGATCY 1 cut(s) 946
MhlI GDGCHC 3 cut(s) 111, 870, 1068
MluCI AATT 8 cut(s) 346, 416, 718, 933, 960, 1027, 1055, 1118
Mly113I GGCGCC 1 cut(s) 244
MlyI GAGTC 1 cut(s) 653
MnlI CCTC 8 cut(s) 143, 193, 514, 535, 804, 889, 931, 1117
MroNI GCCGGC 1 cut(s) 246
MroXI GAANNNNTTC 2 cut(s) 236, 1131
MseI TTAA 7 cut(s) 98, 257, 273, 293, 597, 963, 1026
MspI CCGG 3 cut(s) 51, 247, 358
MspR9I CCNGG 2 cut(s) 358, 649
MunI CAATTG 1 cut(s) 346
Mva1269I GAATGC 1 cut(s) 268
MvaI CCWGG 1 cut(s) 649
MvnI CGCG 1 cut(s) 913
MwoI GCNNNNNNNGC 2 cut(s) 241, 687
NaeI GCCGGC 1 cut(s) 248
NarI GGCGCC 1 cut(s) 244
NciI CCSGG 1 cut(s) 358
NdeII GATC 6 cut(s) 342, 379, 474, 579, 946, 952
NgoMIV GCCGGC 1 cut(s) 246
NlaIII CATG 3 cut(s) 404, 820, 889
NlaIV GGNNCC 2 cut(s) 245, 461
NmuCI GTSAC 2 cut(s) 667, 1031
PctI GAATGC 1 cut(s) 268
PdiI GCCGGC 1 cut(s) 248
PdmI GAANNNNTTC 2 cut(s) 236, 1131
PfeI GAWTC 3 cut(s) 17, 859, 889
PfoI TCCNGGA 1 cut(s) 647
PkrI GCNGC 3 cut(s) 399, 901, 912
PleI GAGTC 1 cut(s) 652
PluTI GGCGCC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 652
PpuMI RGGWCCY 1 cut(s) 968
Psp5II RGGWCCY 1 cut(s) 968
Psp6I CCWGG 1 cut(s) 647
PspGI CCWGG 1 cut(s) 647
PspN4I GGNNCC 2 cut(s) 245, 461
PspPI GGNCC 2 cut(s) 805, 968
PspPPI RGGWCCY 1 cut(s) 968
PstI CTGCAG 1 cut(s) 190
PsuI RGATCY 1 cut(s) 946
RsaI GTAC 1 cut(s) 884
RsaNI GTAC 1 cut(s) 883
SaqAI TTAA 7 cut(s) 98, 257, 273, 293, 597, 963, 1026
SatI GCNGC 3 cut(s) 398, 900, 911
Sau3AI GATC 6 cut(s) 342, 379, 474, 579, 946, 952
Sau96I GGNCC 2 cut(s) 805, 968
SchI GAGTC 1 cut(s) 653
ScrFI CCNGG 2 cut(s) 358, 649
SduI GDGCHC 3 cut(s) 111, 870, 1068
SfaNI GCATC 1 cut(s) 208
SfcI CTRYAG 2 cut(s) 186, 852
SfoI GGCGCC 1 cut(s) 245
SinI GGWCC 2 cut(s) 805, 968
SmlI CTYRAG 1 cut(s) 863
SmoI CTYRAG 1 cut(s) 863
Sse9I AATT 8 cut(s) 346, 416, 718, 933, 960, 1027, 1055, 1118
SsiI CCGC 2 cut(s) 397, 911
SspDI GGCGCC 1 cut(s) 243
SspMI CTAG 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 356, 647
TaaI ACNGT 4 cut(s) 82, 127, 636, 856
TaqI TCGA 3 cut(s) 55, 122, 230
TasI AATT 8 cut(s) 346, 416, 718, 933, 960, 1027, 1055, 1118
TatI WGTACW 1 cut(s) 882
TauI GCSGC 2 cut(s) 400, 913
TfiI GAWTC 3 cut(s) 17, 859, 889
Tru1I TTAA 7 cut(s) 98, 257, 273, 293, 597, 963, 1026
Tru9I TTAA 7 cut(s) 98, 257, 273, 293, 597, 963, 1026
TscAI CASTG 4 cut(s) 373, 709, 801, 861
TseFI GTSAC 2 cut(s) 667, 1031
TseI GCWGC 1 cut(s) 899
Tsp45I GTSAC 2 cut(s) 667, 1031
TspDTI ATGAA 7 cut(s) 17, 428, 814, 854, 902, 1068, 1140
TspGWI ACGGA 1 cut(s) 637
TspRI CASTG 4 cut(s) 373, 709, 801, 861
VneI GTGCAC 1 cut(s) 107
VpaK11BI GGWCC 2 cut(s) 805, 968
XagI CCTNNNNNAGG 1 cut(s) 137
XapI RAATTY 1 cut(s) 718
XbaI TCTAGA 1 cut(s) 8
XmnI GAANNNNTTC 2 cut(s) 236, 1131
XspI CTAG 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.