RchiOBHm_Chr6g0285441

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000965
Physical Location & Seq
Forward (+)
48796046 .. 48796489
444 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25603

Sequence Viewer

Length: 444 bp
ATGAGAGCTCTCAAAGGCATTGCCGTTTTCTATTTGTGTGCAGCTTTAGCCCTAAGTATCTACCCAGGATCCTCTGCCAAAGCTCCTGGTCTTGATCCCACCGACTGGCATGTGCACGTCGTTAACGGTTTCAGTGGGCGAAGAGCACTGGTGGTCCACTGTAAGTCCGACGTCAACGATCTCGGCACCCATACTCTTTATCCTGGTGGCGACTTCAATTGGAATTTCCATGTCAACTTGCCCGGGTCAACGCGTTTCACTTGCGACTTCCATGCAGACAAACAACACGCTTCATTTGATGTGTTTTGGAAGGAGAGTCAACATATGTGGCTCCGGACCAGATGCAATTGGAAAGCGTGCTTTTGGACTGCGAAAGATGATGGGATTTACCTCAAAAACGTTCCAGAGAATCATGACGAGCGTATTCATCCATGGGAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

147

Amino Acids

16.82

Weight (kDa)

7.14

Isoelectric Point (pI)

28.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 37 - 145 6e-27 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 174
AccB1I GGYRCC 1 cut(s) 185
AccB7I CCANNNNNTGG 1 cut(s) 105
AccII CGCG 1 cut(s) 253
AccIII TCCGGA 1 cut(s) 333
AclI AACGTT 1 cut(s) 399
AclWI GGATC 3 cut(s) 63, 76, 89
AcsI RAATTY 1 cut(s) 223
AcyI GRCGYC 1 cut(s) 171
AfiI CCNNNNNNNGG 1 cut(s) 105
AflIII ACRYGT 1 cut(s) 251
AgsI TTSAA 1 cut(s) 217
AjiI CACGTC 1 cut(s) 118
AjnI CCWGG 3 cut(s) 64, 85, 202
AluBI AGCT 3 cut(s) 8, 44, 83
AluI AGCT 3 cut(s) 8, 44, 83
Alw21I GWGCWC 3 cut(s) 10, 117, 148
Alw44I GTGCAC 1 cut(s) 113
AlwI GGATC 3 cut(s) 63, 76, 89
Ama87I CYCGRG 1 cut(s) 242
Aor13HI TCCGGA 1 cut(s) 333
ApaLI GTGCAC 1 cut(s) 113
ApeKI GCWGC 1 cut(s) 41
ApoI RAATTY 1 cut(s) 223
AspS9I GGNCC 2 cut(s) 154, 336
AsuC2I CCSGG 2 cut(s) 243, 244
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 2 cut(s) 154, 336
BaeGI GKGCMC 1 cut(s) 117
BamHI GGATCC 1 cut(s) 68
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 10
Bbv12I GWGCWC 3 cut(s) 10, 117, 148
BbvI GCAGC 1 cut(s) 53
BccI CCATC 1 cut(s) 374
BceAI ACGGC 1 cut(s) 8
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 3 cut(s) 66, 87, 204
BcnI CCSGG 2 cut(s) 243, 244
BisI GCNGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 43
Bme1390I CCNGG 5 cut(s) 66, 87, 204, 243, 244
Bme18I GGWCC 2 cut(s) 154, 336
BmeT110I CYCGRG 1 cut(s) 242
BmgBI CACGTC 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 154, 336
BmiI GGNNCC 3 cut(s) 70, 187, 332
BmrFI CCNGG 5 cut(s) 66, 87, 204, 243, 244
BmsI GCATC 1 cut(s) 332
BpuMI CCSGG 2 cut(s) 243, 244
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 3 cut(s) 64, 242, 431
BsaWI WCCGGW 1 cut(s) 333
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse1I ACTGG 2 cut(s) 110, 153
Bse3DI GCAATG 1 cut(s) 18
BseAI TCCGGA 1 cut(s) 333
BseBI CCWGG 3 cut(s) 66, 87, 204
BseDI CCNNGG 3 cut(s) 64, 242, 431
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 2 cut(s) 110, 153
BseSI GKGCMC 1 cut(s) 117
BseXI GCAGC 1 cut(s) 53
BsgI GTGCAG 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 253
BshNI GGYRCC 1 cut(s) 185
BsiHKAI GWGCWC 3 cut(s) 10, 117, 148
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 2 cut(s) 243, 334
BslI CCNNNNNNNGG 1 cut(s) 105
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 3 cut(s) 10, 117, 148
Bsp13I TCCGGA 1 cut(s) 333
Bsp143I GATC 3 cut(s) 68, 94, 178
Bsp19I CCATGG 1 cut(s) 431
BspEI TCCGGA 1 cut(s) 333
BspFNI CGCG 1 cut(s) 253
BspHI TCATGA 1 cut(s) 412
BspLI GGNNCC 3 cut(s) 70, 187, 332
BspPI GGATC 3 cut(s) 63, 76, 89
BspQI GCTCTTC 1 cut(s) 136
BspT107I GGYRCC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 2 cut(s) 110, 153
BssECI CCNNGG 3 cut(s) 64, 242, 431
BssMI GATC 3 cut(s) 68, 94, 178
BssNI GRCGYC 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 431
Bst2UI CCWGG 3 cut(s) 66, 87, 204
Bst4CI ACNGT 2 cut(s) 128, 161
Bst6I CTCTTC 1 cut(s) 136
BstACI GRCGYC 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 358
BstDEI CTNAG 1 cut(s) 53
BstDSI CCRYGG 1 cut(s) 431
BstF5I GGATG 1 cut(s) 427
BstFNI CGCG 1 cut(s) 253
BstKTI GATC 3 cut(s) 71, 97, 181
BstMBI GATC 3 cut(s) 68, 94, 178
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 3 cut(s) 66, 87, 204
BstNSI RCATGY 1 cut(s) 113
BstSCI CCNGG 5 cut(s) 64, 85, 202, 241, 242
BstSLI GKGCMC 1 cut(s) 117
BstUI CGCG 1 cut(s) 253
BstV1I GCAGC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
BtgI CCRYGG 1 cut(s) 431
BtrI CACGTC 1 cut(s) 118
BtsCI GGATG 1 cut(s) 427
BtsIMutI CAGTG 3 cut(s) 139, 146, 157
Cac8I GCNNGC 1 cut(s) 358
CciI TCATGA 1 cut(s) 412
Cfr13I GGNCC 2 cut(s) 154, 336
Cfr9I CCCGGG 1 cut(s) 242
CspCI CAANNNNNGTGG 2 cut(s) 308, 343
CviAII CATG 6 cut(s) 110, 230, 272, 413, 432, 441
CviJI RGCY 5 cut(s) 8, 44, 50, 83, 331
CviKI_1 RGCY 5 cut(s) 8, 44, 50, 83, 331
DdeI CTNAG 1 cut(s) 53
DpnI GATC 3 cut(s) 70, 96, 180
DpnII GATC 3 cut(s) 68, 94, 178
Eam1104I CTCTTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 136
Ecl136II GAGCTC 1 cut(s) 8
Eco130I CCWWGG 1 cut(s) 431
Eco24I GRGCYC 1 cut(s) 10
Eco47I GGWCC 2 cut(s) 154, 336
Eco53kI GAGCTC 1 cut(s) 8
Eco88I CYCGRG 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 8
EcoRII CCWGG 3 cut(s) 64, 85, 202
EcoT14I CCWWGG 1 cut(s) 431
EcoT38I GRGCYC 1 cut(s) 10
ErhI CCWWGG 1 cut(s) 431
FaeI CATG 6 cut(s) 113, 233, 275, 416, 435, 444
FaiI YATR 9 cut(s) 111, 192, 231, 273, 324, 326, 414, 433, 442
FatI CATG 6 cut(s) 109, 229, 271, 412, 431, 440
FauNDI CATATG 1 cut(s) 324
Fnu4HI GCNGC 1 cut(s) 42
FokI GGATG 1 cut(s) 414
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 1 cut(s) 42
GluI GCNGC 1 cut(s) 42
HapII CCGG 2 cut(s) 243, 334
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 6 cut(s) 113, 233, 275, 416, 435, 444
HincII GTYRAC 5 cut(s) 124, 175, 235, 249, 320
HindII GTYRAC 5 cut(s) 124, 175, 235, 249, 320
HinfI GANTC 2 cut(s) 316, 409
HpaI GTTAAC 1 cut(s) 124
HpaII CCGG 2 cut(s) 243, 334
Hpy166II GTNNAC 7 cut(s) 115, 124, 157, 175, 235, 249, 320
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 4 cut(s) 92, 334, 404, 413
Hpy8I GTNNAC 7 cut(s) 115, 124, 157, 175, 235, 249, 320
Hpy99I CGWCG 2 cut(s) 122, 173
HpyAV CCTTC 1 cut(s) 304
HpyCH4III ACNGT 2 cut(s) 128, 161
HpyCH4IV ACGT 3 cut(s) 117, 171, 399
HpyCH4V TGCA 4 cut(s) 41, 115, 275, 345
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 3 cut(s) 117, 171, 399
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 6 cut(s) 113, 233, 275, 416, 435, 444
Kpn2I TCCGGA 1 cut(s) 333
KspAI GTTAAC 1 cut(s) 124
Kzo9I GATC 3 cut(s) 68, 94, 178
LguI GCTCTTC 1 cut(s) 136
LmnI GCTCC 2 cut(s) 88, 336
Lsp1109I GCAGC 1 cut(s) 53
LweI GCATC 1 cut(s) 332
MaeII ACGT 3 cut(s) 117, 171, 399
MalI GATC 3 cut(s) 70, 96, 180
MboI GATC 3 cut(s) 68, 94, 178
MboII GAAGA 1 cut(s) 153
MfeI CAATTG 2 cut(s) 217, 346
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 3 cut(s) 10, 117, 148
MluCI AATT 3 cut(s) 217, 223, 346
MluI ACGCGT 1 cut(s) 251
MlyI GAGTC 1 cut(s) 325
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 2 cut(s) 82, 401
MroI TCCGGA 1 cut(s) 333
MseI TTAA 1 cut(s) 123
MspI CCGG 2 cut(s) 243, 334
MspR9I CCNGG 5 cut(s) 66, 87, 204, 243, 244
MunI CAATTG 2 cut(s) 217, 346
MvaI CCWGG 3 cut(s) 66, 87, 204
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 1 cut(s) 47
NciI CCSGG 2 cut(s) 243, 244
NcoI CCATGG 1 cut(s) 431
NdeI CATATG 1 cut(s) 324
NdeII GATC 3 cut(s) 68, 94, 178
NlaIII CATG 6 cut(s) 113, 233, 275, 416, 435, 444
NlaIV GGNNCC 3 cut(s) 70, 187, 332
NmeAIII GCCGAG 1 cut(s) 162
NspI RCATGY 1 cut(s) 113
PagI TCATGA 1 cut(s) 412
PciSI GCTCTTC 1 cut(s) 136
PfeI GAWTC 1 cut(s) 409
PflMI CCANNNNNTGG 1 cut(s) 105
PkrI GCNGC 1 cut(s) 43
PleI GAGTC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 10
Psp1406I AACGTT 1 cut(s) 399
Psp6I CCWGG 3 cut(s) 64, 85, 202
PspGI CCWGG 3 cut(s) 64, 85, 202
PspN4I GGNNCC 3 cut(s) 70, 187, 332
PspPI GGNCC 2 cut(s) 154, 336
PsuI RGATCY 1 cut(s) 68
SacI GAGCTC 1 cut(s) 10
SapI GCTCTTC 1 cut(s) 136
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 1 cut(s) 42
Sau3AI GATC 3 cut(s) 68, 94, 178
Sau96I GGNCC 2 cut(s) 154, 336
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 5 cut(s) 66, 87, 204, 243, 244
SduI GDGCHC 3 cut(s) 10, 117, 148
SetI ASST 7 cut(s) 10, 46, 85, 120, 174, 393, 402
SfaNI GCATC 1 cut(s) 332
SinI GGWCC 2 cut(s) 154, 336
SmaI CCCGGG 1 cut(s) 244
Sse9I AATT 3 cut(s) 217, 223, 346
SstI GAGCTC 1 cut(s) 10
StyD4I CCNGG 5 cut(s) 64, 85, 202, 241, 242
StyI CCWWGG 1 cut(s) 431
TaaI ACNGT 2 cut(s) 128, 161
TaiI ACGT 3 cut(s) 120, 174, 402
TasI AATT 3 cut(s) 217, 223, 346
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TscAI CASTG 3 cut(s) 139, 153, 164
TseI GCWGC 1 cut(s) 41
TspDTI ATGAA 2 cut(s) 282, 416
TspMI CCCGGG 1 cut(s) 242
TspRI CASTG 3 cut(s) 139, 153, 164
Van91I CCANNNNNTGG 1 cut(s) 105
VneI GTGCAC 1 cut(s) 113
VpaK11BI GGWCC 2 cut(s) 154, 336
XapI RAATTY 1 cut(s) 223
XceI RCATGY 1 cut(s) 113
XmaI CCCGGG 1 cut(s) 242
ZraI GACGTC 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.