Rroxscaffold_7G00183150

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000965
Physical Location & Seq
Reverse (-)
22194704 .. 22207349
12646 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00183150.1

Sequence Viewer

Length: 855 bp
ATGAATACTGCTTGTCTTACTTATCTGCCTGAGCCAACCATGGGTCACACATGGTTGGCTAGGGGAGTACACAGTATTCATTGTAAATGGCTTGGGAAACAATCAGCTTTAACTCTGAATATCCTGTGTCCCGAAAACTCTGCCGAGATCGACTGGGGTATTGATCCCACGACATGGCATGTGTACGTCTTGAACGGATTGGGTGCACATAGAGCATTGGAGATTCATTGTAAGTCCAAAGACGACGATTTGGGCACCCATACACTTACTCCAACCCTCAAATCCCACAAACCTCCGCCACCCACCTTGAAGCCACGGACTCCAAGAACTCGGAGCGGAGTCCCCACCCCCGAAGCCGGCGGAATCAACTCGAACCGACCATTGGAAGTGACTGAACCGGCCCCAAGAAGAATGCATATCTTCCCGGATCCTCTCGCCAAAGCACCTGGTCTTGATCCCAACGATCGGCATGTGCACGTTGTTAACGGTTTCGGTGGGCGAAGAGCACCGGTGGTCCACCGTAAGTCCGACGTCAACGATCTCGGCACCCATACTCTTTATCCTGGTGGCGACTTCAATTGGAATTTCCATGTCAACTTGCACGGGTCAACGCGTTTCACTTGCGACTTCCATGCGGACAAACAACATGCTTCATTTGATGTGTTTTGGAAGGAGAGTCAACATATGTGGCTCCGGACCAGATGCAATTGGAAAGCGTGCTTTTGGACTGCGAAAGATGATGGGATTTACCTCAAAATGTTCCGGAGAATCGTGATGAGCGTATTCATCCATGGGAAACATGACGGTGATATTGAAAAGGTGGAAATGTTTAAATTTGCACTTCATGTGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

284

Amino Acids

32.21

Weight (kDa)

8.95

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 60 - 91 1.2e-06 Plant self-incompatibility protein S1
Self-incomp_S1 PF05938 157 - 251 2.4e-20 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 534
AccB1I GGYRCC 2 cut(s) 254, 545
AccB7I CCANNNNNTGG 1 cut(s) 174
AccBSI CCGCTC 1 cut(s) 336
AccII CGCG 1 cut(s) 613
AccIII TCCGGA 2 cut(s) 693, 762
AciI CCGC 4 cut(s) 296, 336, 360, 635
AclWI GGATC 4 cut(s) 158, 422, 435, 449
AcsI RAATTY 2 cut(s) 583, 833
AcyI GRCGYC 1 cut(s) 531
AfaI GTAC 2 cut(s) 69, 185
AfiI CCNNNNNNNGG 5 cut(s) 41, 174, 356, 382, 465
AflIII ACRYGT 1 cut(s) 611
AgeI ACCGGT 1 cut(s) 508
AgsI TTSAA 4 cut(s) 193, 310, 577, 815
AjnI CCWGG 2 cut(s) 445, 562
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 1 cut(s) 107
AluI AGCT 1 cut(s) 107
Alw21I GWGCWC 3 cut(s) 208, 477, 508
Alw44I GTGCAC 2 cut(s) 204, 473
AlwI GGATC 4 cut(s) 158, 422, 435, 449
Aor13HI TCCGGA 2 cut(s) 693, 762
AoxI GGCC 1 cut(s) 399
ApaLI GTGCAC 2 cut(s) 204, 473
ApoI RAATTY 2 cut(s) 583, 833
AsiGI ACCGGT 1 cut(s) 508
AspS9I GGNCC 3 cut(s) 400, 514, 696
AsuC2I CCSGG 1 cut(s) 425
AsuHPI GGTGA 1 cut(s) 818
AvaII GGWCC 2 cut(s) 514, 696
BaeGI GKGCMC 3 cut(s) 208, 257, 477
BamHI GGATCC 1 cut(s) 427
BanI GGYRCC 2 cut(s) 254, 545
Bbv12I GWGCWC 3 cut(s) 208, 477, 508
BccI CCATC 1 cut(s) 734
BcgI CGANNNNNNTGC 4 cut(s) 122, 156, 614, 648
BciT130I CCWGG 2 cut(s) 447, 564
BcnI CCSGG 1 cut(s) 425
BfaI CTAG 1 cut(s) 60
Bme1390I CCNGG 3 cut(s) 425, 447, 564
Bme18I GGWCC 2 cut(s) 514, 696
BmgT120I GGNCC 3 cut(s) 400, 514, 696
BmiI GGNNCC 5 cut(s) 256, 402, 429, 547, 692
BmrFI CCNGG 3 cut(s) 425, 447, 564
BmrI ACTGGG 1 cut(s) 163
BmsI GCATC 1 cut(s) 692
BmuI ACTGGG 1 cut(s) 163
Bpu10I CCTNAGC 1 cut(s) 30
BpuMI CCSGG 1 cut(s) 425
BsaHI GRCGYC 1 cut(s) 531
BsaJI CCNNGG 3 cut(s) 39, 314, 790
BsaWI WCCGGW 3 cut(s) 508, 693, 762
BsaXI ACNNNNNCTCC 2 cut(s) 253, 283
Bsc4I CCNNNNNNNGG 5 cut(s) 41, 174, 356, 382, 465
Bse118I RCCGGY 3 cut(s) 356, 397, 508
Bse1I ACTGG 1 cut(s) 158
BseAI TCCGGA 2 cut(s) 693, 762
BseBI CCWGG 2 cut(s) 447, 564
BseDI CCNNGG 3 cut(s) 39, 314, 790
BseGI GGATG 1 cut(s) 786
BseLI CCNNNNNNNGG 5 cut(s) 41, 174, 356, 382, 465
BseMII CTCAG 1 cut(s) 21
BseNI ACTGG 1 cut(s) 158
BseSI GKGCMC 3 cut(s) 208, 257, 477
Bsh1236I CGCG 1 cut(s) 613
Bsh1285I CGRYCG 1 cut(s) 466
BshFI GGCC 1 cut(s) 401
BshNI GGYRCC 2 cut(s) 254, 545
BshTI ACCGGT 1 cut(s) 508
BsiEI CGRYCG 1 cut(s) 466
BsiHKAI GWGCWC 3 cut(s) 208, 477, 508
BsiSI CCGG 6 cut(s) 357, 398, 425, 509, 694, 763
BslFI GGGAC 2 cut(s) 114, 326
BslI CCNNNNNNNGG 5 cut(s) 41, 174, 356, 382, 465
BsmFI GGGAC 2 cut(s) 114, 326
BsmI GAATGC 1 cut(s) 417
BsnI GGCC 1 cut(s) 401
Bsp1286I GDGCHC 4 cut(s) 208, 257, 477, 508
Bsp13I TCCGGA 2 cut(s) 693, 762
Bsp143I GATC 6 cut(s) 147, 163, 427, 454, 463, 538
Bsp19I CCATGG 2 cut(s) 39, 790
BspACI CCGC 4 cut(s) 296, 336, 360, 635
BspANI GGCC 1 cut(s) 401
BspCNI CTCAG 1 cut(s) 22
BspEI TCCGGA 2 cut(s) 693, 762
BspFNI CGCG 1 cut(s) 613
BspLI GGNNCC 5 cut(s) 256, 402, 429, 547, 692
BspPI GGATC 4 cut(s) 158, 422, 435, 449
BspQI GCTCTTC 1 cut(s) 496
BspT107I GGYRCC 2 cut(s) 254, 545
BsrBI CCGCTC 1 cut(s) 336
BsrFI RCCGGY 3 cut(s) 356, 397, 508
BsrI ACTGG 1 cut(s) 158
BssAI RCCGGY 3 cut(s) 356, 397, 508
BssECI CCNNGG 3 cut(s) 39, 314, 790
BssMI GATC 6 cut(s) 147, 163, 427, 454, 463, 538
BssNI GRCGYC 1 cut(s) 531
BssT1I CCWWGG 2 cut(s) 39, 790
Bst2UI CCWGG 2 cut(s) 447, 564
Bst4CI ACNGT 4 cut(s) 74, 488, 521, 806
Bst6I CTCTTC 1 cut(s) 496
BstACI GRCGYC 1 cut(s) 531
BstC8I GCNNGC 2 cut(s) 358, 718
BstDEI CTNAG 1 cut(s) 30
BstDSI CCRYGG 3 cut(s) 39, 314, 790
BstF5I GGATG 1 cut(s) 786
BstFNI CGCG 1 cut(s) 613
BstKTI GATC 6 cut(s) 150, 166, 430, 457, 466, 541
BstMBI GATC 6 cut(s) 147, 163, 427, 454, 463, 538
BstMCI CGRYCG 1 cut(s) 466
BstMWI GCNNNNNNNGC 1 cut(s) 212
BstNI CCWGG 2 cut(s) 447, 564
BstNSI RCATGY 3 cut(s) 182, 473, 650
BstSCI CCNGG 3 cut(s) 423, 445, 562
BstSLI GKGCMC 3 cut(s) 208, 257, 477
BstUI CGCG 1 cut(s) 613
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
BsuRI GGCC 1 cut(s) 401
BtgI CCRYGG 3 cut(s) 39, 314, 790
BtsCI GGATG 1 cut(s) 786
Cac8I GCNNGC 2 cut(s) 358, 718
Cfr10I RCCGGY 3 cut(s) 356, 397, 508
Cfr13I GGNCC 3 cut(s) 400, 514, 696
CsiI ACCWGGT 1 cut(s) 445
Csp6I GTAC 2 cut(s) 68, 184
CspAI ACCGGT 1 cut(s) 508
CspCI CAANNNNNGTGG 4 cut(s) 288, 323, 668, 703
CviJI RGCY 8 cut(s) 34, 59, 91, 107, 313, 356, 401, 691
CviKI_1 RGCY 8 cut(s) 34, 59, 91, 107, 313, 356, 401, 691
CviQI GTAC 2 cut(s) 68, 184
DdeI CTNAG 1 cut(s) 30
DpnI GATC 6 cut(s) 149, 165, 429, 456, 465, 540
DpnII GATC 6 cut(s) 147, 163, 427, 454, 463, 538
DraI TTTAAA 1 cut(s) 832
Eam1104I CTCTTC 1 cut(s) 496
EarI CTCTTC 1 cut(s) 496
EciI GGCGGA 2 cut(s) 285, 375
Eco130I CCWWGG 2 cut(s) 39, 790
Eco47I GGWCC 2 cut(s) 514, 696
EcoRII CCWGG 2 cut(s) 445, 562
EcoT14I CCWWGG 2 cut(s) 39, 790
EcoT22I ATGCAT 1 cut(s) 417
ErhI CCWWGG 2 cut(s) 39, 790
FaqI GGGAC 2 cut(s) 114, 326
FauNDI CATATG 1 cut(s) 684
FokI GGATG 1 cut(s) 773
FspBI CTAG 1 cut(s) 60
HaeIII GGCC 1 cut(s) 401
HapII CCGG 6 cut(s) 357, 398, 425, 509, 694, 763
Hin1I GRCGYC 1 cut(s) 531
HincII GTYRAC 5 cut(s) 484, 535, 595, 609, 680
HindII GTYRAC 5 cut(s) 484, 535, 595, 609, 680
HinfI GANTC 6 cut(s) 223, 319, 339, 363, 676, 768
HpaI GTTAAC 1 cut(s) 484
HpaII CCGG 6 cut(s) 357, 398, 425, 509, 694, 763
HphI GGTGA 1 cut(s) 818
Hpy188I TCNGA 3 cut(s) 117, 333, 529
Hpy188III TCNNGA 6 cut(s) 131, 190, 452, 694, 763, 772
Hpy99I CGWCG 2 cut(s) 248, 533
HpyAV CCTTC 1 cut(s) 664
HpyCH4III ACNGT 4 cut(s) 74, 488, 521, 806
HpyCH4IV ACGT 3 cut(s) 186, 477, 531
HpyCH4V TGCA 6 cut(s) 206, 415, 475, 601, 705, 839
HpyF10VI GCNNNNNNNGC 1 cut(s) 212
HpyF3I CTNAG 1 cut(s) 30
HpySE526I ACGT 3 cut(s) 186, 477, 531
Hsp92I GRCGYC 1 cut(s) 531
Kpn2I TCCGGA 2 cut(s) 693, 762
KroI GCCGGC 1 cut(s) 356
KroNI GCCGGC 1 cut(s) 358
KspAI GTTAAC 1 cut(s) 484
Kzo9I GATC 6 cut(s) 147, 163, 427, 454, 463, 538
LguI GCTCTTC 1 cut(s) 496
LmnI GCTCC 2 cut(s) 333, 696
LweI GCATC 1 cut(s) 692
MabI ACCWGGT 1 cut(s) 445
MaeI CTAG 1 cut(s) 60
MaeII ACGT 3 cut(s) 186, 477, 531
MaeIII GTNAC 2 cut(s) 44, 388
MalI GATC 6 cut(s) 149, 165, 429, 456, 465, 540
MbiI CCGCTC 1 cut(s) 336
MboI GATC 6 cut(s) 147, 163, 427, 454, 463, 538
MboII GAAGA 3 cut(s) 412, 420, 513
MfeI CAATTG 2 cut(s) 577, 706
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 4 cut(s) 208, 257, 477, 508
MluCI AATT 4 cut(s) 577, 583, 706, 833
MluI ACGCGT 1 cut(s) 611
MlyI GAGTC 3 cut(s) 313, 348, 685
MmeI TCCRAC 2 cut(s) 296, 552
MnlI CCTC 4 cut(s) 287, 303, 441, 761
Mph1103I ATGCAT 1 cut(s) 417
MroI TCCGGA 2 cut(s) 693, 762
MroNI GCCGGC 1 cut(s) 356
MseI TTAA 4 cut(s) 110, 483, 831, 853
MslI CAYNNNNRTG 1 cut(s) 804
MspI CCGG 6 cut(s) 357, 398, 425, 509, 694, 763
MspR9I CCNGG 3 cut(s) 425, 447, 564
MunI CAATTG 2 cut(s) 577, 706
Mva1269I GAATGC 1 cut(s) 417
MvaI CCWGG 2 cut(s) 447, 564
MvnI CGCG 1 cut(s) 613
MwoI GCNNNNNNNGC 1 cut(s) 212
NaeI GCCGGC 1 cut(s) 358
NciI CCSGG 1 cut(s) 425
NcoI CCATGG 2 cut(s) 39, 790
NdeI CATATG 1 cut(s) 684
NdeII GATC 6 cut(s) 147, 163, 427, 454, 463, 538
NgoMIV GCCGGC 1 cut(s) 356
NlaIV GGNNCC 5 cut(s) 256, 402, 429, 547, 692
NmeAIII GCCGAG 2 cut(s) 169, 522
NmuCI GTSAC 2 cut(s) 44, 388
NsiI ATGCAT 1 cut(s) 417
NspI RCATGY 3 cut(s) 182, 473, 650
PciSI GCTCTTC 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 777
PctI GAATGC 1 cut(s) 417
PdiI GCCGGC 1 cut(s) 358
PfeI GAWTC 3 cut(s) 223, 363, 768
PflMI CCANNNNNTGG 1 cut(s) 174
PfoI TCCNGGA 1 cut(s) 423
PinAI ACCGGT 1 cut(s) 508
Ple19I CGATCG 1 cut(s) 466
PleI GAGTC 3 cut(s) 313, 347, 684
PpsI GAGTC 3 cut(s) 313, 347, 684
Psp6I CCWGG 2 cut(s) 445, 562
PspGI CCWGG 2 cut(s) 445, 562
PspN4I GGNNCC 5 cut(s) 256, 402, 429, 547, 692
PspPI GGNCC 3 cut(s) 400, 514, 696
PsuI RGATCY 1 cut(s) 427
PvuI CGATCG 1 cut(s) 466
RsaI GTAC 2 cut(s) 69, 185
RsaNI GTAC 2 cut(s) 68, 184
RseI CAYNNNNRTG 1 cut(s) 804
SapI GCTCTTC 1 cut(s) 496
SaqAI TTAA 4 cut(s) 110, 483, 831, 853
Sau3AI GATC 6 cut(s) 147, 163, 427, 454, 463, 538
Sau96I GGNCC 3 cut(s) 400, 514, 696
SchI GAGTC 3 cut(s) 313, 348, 685
ScrFI CCNGG 3 cut(s) 425, 447, 564
SduI GDGCHC 4 cut(s) 208, 257, 477, 508
SetI ASST 9 cut(s) 109, 189, 295, 308, 448, 480, 534, 753, 822
SexAI ACCWGGT 1 cut(s) 445
SfaNI GCATC 1 cut(s) 692
SgrAI CRCCGGYG 1 cut(s) 508
SinI GGWCC 2 cut(s) 514, 696
SmiMI CAYNNNNRTG 1 cut(s) 804
Sse9I AATT 4 cut(s) 577, 583, 706, 833
SsiI CCGC 4 cut(s) 296, 336, 360, 635
SspMI CTAG 1 cut(s) 60
StyD4I CCNGG 3 cut(s) 423, 445, 562
StyI CCWWGG 2 cut(s) 39, 790
TaaI ACNGT 4 cut(s) 74, 488, 521, 806
TaiI ACGT 3 cut(s) 189, 480, 534
TaqI TCGA 2 cut(s) 150, 371
TasI AATT 4 cut(s) 577, 583, 706, 833
TatI WGTACW 1 cut(s) 67
TfiI GAWTC 3 cut(s) 223, 363, 768
Tru1I TTAA 4 cut(s) 110, 483, 831, 853
Tru9I TTAA 4 cut(s) 110, 483, 831, 853
TseFI GTSAC 2 cut(s) 44, 388
Tsp45I GTSAC 2 cut(s) 44, 388
TspDTI ATGAA 6 cut(s) 17, 68, 215, 642, 775, 833
TspGWI ACGGA 2 cut(s) 210, 331
Van91I CCANNNNNTGG 1 cut(s) 174
VneI GTGCAC 2 cut(s) 204, 473
VpaK11BI GGWCC 2 cut(s) 514, 696
XapI RAATTY 2 cut(s) 583, 833
XceI RCATGY 3 cut(s) 182, 473, 650
XspI CTAG 1 cut(s) 60
ZraI GACGTC 1 cut(s) 532
Zsp2I ATGCAT 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.