RchiOBHm_Chr6g0285431

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000965
Physical Location & Seq
Forward (+)
48782152 .. 48782595
444 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25602

Sequence Viewer

Length: 444 bp
ATGAGAGCTCTCAAAGGCATTGTCGTTTTCTATTTGTGTGCAGCTTTAGCCCTGAGTATCTGCCCGGGATCCTCTGCCAAATGGCCCGGTCTTGATCCCACCAACTGGCATGTGCACGTCGTTAACGGTTTCGGTGGACAAAGGGCATTGGTTGTCCATTGTTGGTCTGACCTCGACGATCTTGGCACCCATACTCTTTATCCTGGTGGCGACTTCAACTGGCATTTCCATGTCAACTTGCCCGGGACAACGCGTTTCACTTGCGACTTCCATGCAGACAAACAACACGCTTTGTTTGATGTGTTTTGGAAGGAGAGTGAACATATGTGGCTTCGGACCCATTGCAATTGGAAAGCGTGTTTTTGGATTGCGAAAGATGATGGGATTTACCTCAAAAACGTTCCGGAGAATCGTGACGAGCGTATTCATCCATGGGAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

147

Amino Acids

17.02

Weight (kDa)

6.39

Isoelectric Point (pI)

25.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 37 - 145 4.5e-27 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 185
AccB7I CCANNNNNTGG 1 cut(s) 105
AccII CGCG 1 cut(s) 253
AccIII TCCGGA 1 cut(s) 403
AclI AACGTT 1 cut(s) 399
AclWI GGATC 3 cut(s) 63, 76, 89
AfiI CCNNNNNNNGG 1 cut(s) 105
AflIII ACRYGT 1 cut(s) 251
AgsI TTSAA 1 cut(s) 217
AjiI CACGTC 1 cut(s) 118
AjnI CCWGG 1 cut(s) 202
AluBI AGCT 2 cut(s) 8, 44
AluI AGCT 2 cut(s) 8, 44
Alw21I GWGCWC 2 cut(s) 10, 117
Alw44I GTGCAC 1 cut(s) 113
AlwI GGATC 3 cut(s) 63, 76, 89
Ama87I CYCGRG 2 cut(s) 64, 242
Aor13HI TCCGGA 1 cut(s) 403
AoxI GGCC 1 cut(s) 83
ApaLI GTGCAC 1 cut(s) 113
ApeKI GCWGC 1 cut(s) 41
ArsI GACNNNNNNTTYG 1 cut(s) 38
AspS9I GGNCC 2 cut(s) 84, 336
AsuC2I CCSGG 5 cut(s) 65, 66, 87, 243, 244
AvaI CYCGRG 2 cut(s) 64, 242
AvaII GGWCC 1 cut(s) 336
BaeGI GKGCMC 1 cut(s) 117
BamHI GGATCC 1 cut(s) 68
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 10
Bbv12I GWGCWC 2 cut(s) 10, 117
BbvI GCAGC 1 cut(s) 53
BccI CCATC 1 cut(s) 374
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 1 cut(s) 204
BcnI CCSGG 5 cut(s) 65, 66, 87, 243, 244
BisI GCNGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 43
Bme1390I CCNGG 6 cut(s) 65, 66, 87, 204, 243, 244
Bme18I GGWCC 1 cut(s) 336
BmeT110I CYCGRG 2 cut(s) 64, 242
BmgBI CACGTC 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 84, 336
BmiI GGNNCC 3 cut(s) 70, 187, 338
BmrFI CCNGG 6 cut(s) 65, 66, 87, 204, 243, 244
BpuMI CCSGG 5 cut(s) 65, 66, 87, 243, 244
BsaJI CCNNGG 3 cut(s) 64, 242, 431
BsaWI WCCGGW 1 cut(s) 403
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse1I ACTGG 2 cut(s) 110, 224
Bse3DI GCAATG 1 cut(s) 340
BseAI TCCGGA 1 cut(s) 403
BseBI CCWGG 1 cut(s) 204
BseDI CCNNGG 3 cut(s) 64, 242, 431
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMI GCAATG 1 cut(s) 340
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 2 cut(s) 110, 224
BseSI GKGCMC 1 cut(s) 117
BseXI GCAGC 1 cut(s) 53
BsgI GTGCAG 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 253
BshFI GGCC 1 cut(s) 85
BshNI GGYRCC 1 cut(s) 185
BsiHKAI GWGCWC 2 cut(s) 10, 117
BsiHKCI CYCGRG 2 cut(s) 64, 242
BsiSI CCGG 4 cut(s) 65, 87, 243, 404
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 1 cut(s) 105
BsmFI GGGAC 1 cut(s) 259
BsnI GGCC 1 cut(s) 85
BsoBI CYCGRG 2 cut(s) 64, 242
Bsp1286I GDGCHC 2 cut(s) 10, 117
Bsp13I TCCGGA 1 cut(s) 403
Bsp143I GATC 3 cut(s) 68, 94, 178
Bsp19I CCATGG 1 cut(s) 431
BspANI GGCC 1 cut(s) 85
BspCNI CTCAG 1 cut(s) 45
BspEI TCCGGA 1 cut(s) 403
BspFNI CGCG 1 cut(s) 253
BspLI GGNNCC 3 cut(s) 70, 187, 338
BspPI GGATC 3 cut(s) 63, 76, 89
BspT107I GGYRCC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 340
BsrI ACTGG 2 cut(s) 110, 224
BssECI CCNNGG 3 cut(s) 64, 242, 431
BssMI GATC 3 cut(s) 68, 94, 178
BssT1I CCWWGG 1 cut(s) 431
Bst2UI CCWGG 1 cut(s) 204
Bst4CI ACNGT 1 cut(s) 128
BstDEI CTNAG 1 cut(s) 53
BstDSI CCRYGG 1 cut(s) 431
BstF5I GGATG 1 cut(s) 427
BstFNI CGCG 1 cut(s) 253
BstKTI GATC 3 cut(s) 71, 97, 181
BstMBI GATC 3 cut(s) 68, 94, 178
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 1 cut(s) 204
BstNSI RCATGY 1 cut(s) 113
BstSCI CCNGG 6 cut(s) 63, 64, 85, 202, 241, 242
BstSLI GKGCMC 1 cut(s) 117
BstUI CGCG 1 cut(s) 253
BstV1I GCAGC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
BsuRI GGCC 1 cut(s) 85
BtgI CCRYGG 1 cut(s) 431
BtrI CACGTC 1 cut(s) 118
BtsCI GGATG 1 cut(s) 427
Cfr13I GGNCC 2 cut(s) 84, 336
Cfr9I CCCGGG 2 cut(s) 64, 242
CviAII CATG 4 cut(s) 110, 230, 272, 432
CviJI RGCY 5 cut(s) 8, 44, 50, 85, 331
CviKI_1 RGCY 5 cut(s) 8, 44, 50, 85, 331
DdeI CTNAG 1 cut(s) 53
DpnI GATC 3 cut(s) 70, 96, 180
DpnII GATC 3 cut(s) 68, 94, 178
Ecl136II GAGCTC 1 cut(s) 8
Eco130I CCWWGG 1 cut(s) 431
Eco24I GRGCYC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 336
Eco53kI GAGCTC 1 cut(s) 8
Eco88I CYCGRG 2 cut(s) 64, 242
EcoICRI GAGCTC 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 202
EcoT14I CCWWGG 1 cut(s) 431
EcoT38I GRGCYC 1 cut(s) 10
ErhI CCWWGG 1 cut(s) 431
FaeI CATG 4 cut(s) 113, 233, 275, 435
FaiI YATR 7 cut(s) 111, 192, 231, 273, 324, 326, 433
FaqI GGGAC 1 cut(s) 259
FatI CATG 4 cut(s) 109, 229, 271, 431
FauNDI CATATG 1 cut(s) 324
Fnu4HI GCNGC 1 cut(s) 42
FokI GGATG 1 cut(s) 414
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 1 cut(s) 42
GluI GCNGC 1 cut(s) 42
HaeIII GGCC 1 cut(s) 85
HapII CCGG 4 cut(s) 65, 87, 243, 404
Hin1II CATG 4 cut(s) 113, 233, 275, 435
HincII GTYRAC 2 cut(s) 124, 235
HindII GTYRAC 2 cut(s) 124, 235
HinfI GANTC 1 cut(s) 409
HpaI GTTAAC 1 cut(s) 124
HpaII CCGG 4 cut(s) 65, 87, 243, 404
Hpy166II GTNNAC 5 cut(s) 115, 124, 137, 235, 320
Hpy188I TCNGA 2 cut(s) 169, 336
Hpy188III TCNNGA 3 cut(s) 92, 404, 413
Hpy8I GTNNAC 5 cut(s) 115, 124, 137, 235, 320
Hpy99I CGWCG 2 cut(s) 122, 179
HpyAV CCTTC 1 cut(s) 304
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4IV ACGT 2 cut(s) 117, 399
HpyCH4V TGCA 4 cut(s) 41, 115, 275, 345
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 2 cut(s) 117, 399
Hsp92II CATG 4 cut(s) 113, 233, 275, 435
Kpn2I TCCGGA 1 cut(s) 403
KspAI GTTAAC 1 cut(s) 124
Kzo9I GATC 3 cut(s) 68, 94, 178
LpnPI CCDG 9 cut(s) 65, 78, 91, 100, 189, 205, 216, 256, 417
Lsp1109I GCAGC 1 cut(s) 53
MaeII ACGT 2 cut(s) 117, 399
MaeIII GTNAC 1 cut(s) 413
MalI GATC 3 cut(s) 70, 96, 180
MboI GATC 3 cut(s) 68, 94, 178
MfeI CAATTG 1 cut(s) 346
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 2 cut(s) 10, 117
MluCI AATT 1 cut(s) 346
MluI ACGCGT 1 cut(s) 251
MnlI CCTC 3 cut(s) 82, 182, 401
MroI TCCGGA 1 cut(s) 403
MseI TTAA 1 cut(s) 123
MslI CAYNNNNRTG 1 cut(s) 228
MspI CCGG 4 cut(s) 65, 87, 243, 404
MspR9I CCNGG 6 cut(s) 65, 66, 87, 204, 243, 244
MunI CAATTG 1 cut(s) 346
MvaI CCWGG 1 cut(s) 204
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 1 cut(s) 47
NciI CCSGG 5 cut(s) 65, 66, 87, 243, 244
NcoI CCATGG 1 cut(s) 431
NdeI CATATG 1 cut(s) 324
NdeII GATC 3 cut(s) 68, 94, 178
NlaIII CATG 4 cut(s) 113, 233, 275, 435
NlaIV GGNNCC 3 cut(s) 70, 187, 338
NmuCI GTSAC 1 cut(s) 413
NspI RCATGY 1 cut(s) 113
PcsI WCGNNNNNNNCGW 1 cut(s) 418
PfeI GAWTC 1 cut(s) 409
PflMI CCANNNNNTGG 1 cut(s) 105
PkrI GCNGC 1 cut(s) 43
Psp124BI GAGCTC 1 cut(s) 10
Psp1406I AACGTT 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 202
PspGI CCWGG 1 cut(s) 202
PspN4I GGNNCC 3 cut(s) 70, 187, 338
PspPI GGNCC 2 cut(s) 84, 336
PsuI RGATCY 1 cut(s) 68
RseI CAYNNNNRTG 1 cut(s) 228
SacI GAGCTC 1 cut(s) 10
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 1 cut(s) 42
Sau3AI GATC 3 cut(s) 68, 94, 178
Sau96I GGNCC 2 cut(s) 84, 336
ScrFI CCNGG 6 cut(s) 65, 66, 87, 204, 243, 244
SduI GDGCHC 2 cut(s) 10, 117
SetI ASST 6 cut(s) 10, 46, 120, 174, 393, 402
SinI GGWCC 1 cut(s) 336
SmaI CCCGGG 2 cut(s) 66, 244
SmiMI CAYNNNNRTG 1 cut(s) 228
Sse9I AATT 1 cut(s) 346
SstI GAGCTC 1 cut(s) 10
StyD4I CCNGG 6 cut(s) 63, 64, 85, 202, 241, 242
StyI CCWWGG 1 cut(s) 431
TaaI ACNGT 1 cut(s) 128
TaiI ACGT 2 cut(s) 120, 402
TaqI TCGA 1 cut(s) 174
TasI AATT 1 cut(s) 346
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 1 cut(s) 41
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 1 cut(s) 416
TspMI CCCGGG 2 cut(s) 64, 242
Van91I CCANNNNNTGG 1 cut(s) 105
VneI GTGCAC 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 336
XceI RCATGY 1 cut(s) 113
XmaI CCCGGG 2 cut(s) 64, 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.