RchiOBHm_Chr6g0285421

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000965
Physical Location & Seq
Forward (+)
48774757 .. 48775200
444 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25601

Sequence Viewer

Length: 444 bp
ATGGCAGCTCTCAAAAGCATTGTCGTTCTCTATTTGTGTGCCGCTTTAGCCCTGAGTATCTTCCCGGAATCCTCTGCCAAATGGCCTGGTCTTGATCCCACCAACTGGCATGTGCACGTCGTTAACGGTTTCGGTGGACAAAGAGCATTGGTTGTCCATTGTTGGTCCGACCTCGACGATCTTGGCATCCATACTCTTTATGTTGGTGGTGACTTCAACTGGCATTTCCATGTCAACTTGCCCGGAACAACGCGTTTTACTTGCGACTTCCATGCAGACAAACAACATGCTTTGTTTGATGTGTTTTGGAAGGAGAGTAAACATATGTGGCTTCGGACCAGATGTGACTGGAAAGCGTGCTTTTGGATTGCTAAAGACGATGGGATTTACCTCAAGAACATTCCGGCGAGTCGTGACGAGCTTGTTCATCCATGGGAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

147

Amino Acids

16.92

Weight (kDa)

6.33

Isoelectric Point (pI)

30.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 37 - 145 4.5e-27 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 105
AccII CGCG 1 cut(s) 253
AciI CCGC 1 cut(s) 42
AclWI GGATC 1 cut(s) 89
AfiI CCNNNNNNNGG 1 cut(s) 105
AflIII ACRYGT 1 cut(s) 251
AgsI TTSAA 1 cut(s) 217
AjiI CACGTC 1 cut(s) 118
AjnI CCWGG 1 cut(s) 85
AluBI AGCT 2 cut(s) 8, 421
AluI AGCT 2 cut(s) 8, 421
Alw21I GWGCWC 1 cut(s) 117
Alw44I GTGCAC 1 cut(s) 113
AlwI GGATC 1 cut(s) 89
AoxI GGCC 1 cut(s) 83
ApaLI GTGCAC 1 cut(s) 113
ApeKI GCWGC 1 cut(s) 5
ArsI GACNNNNNNTTYG 1 cut(s) 38
AspS9I GGNCC 2 cut(s) 165, 336
AsuC2I CCSGG 2 cut(s) 65, 243
AsuHPI GGTGA 1 cut(s) 221
AvaII GGWCC 2 cut(s) 165, 336
BaeGI GKGCMC 1 cut(s) 117
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 1 cut(s) 17
BccI CCATC 1 cut(s) 374
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 1 cut(s) 87
BcnI CCSGG 2 cut(s) 65, 243
BisI GCNGC 2 cut(s) 6, 42
BlsI GCNGC 2 cut(s) 7, 43
Bme1390I CCNGG 3 cut(s) 65, 87, 243
Bme18I GGWCC 2 cut(s) 165, 336
BmgBI CACGTC 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 165, 336
BmrFI CCNGG 3 cut(s) 65, 87, 243
BmsI GCATC 1 cut(s) 195
BpuEI CTTGAG 1 cut(s) 377
BpuMI CCSGG 2 cut(s) 65, 243
BsaJI CCNNGG 1 cut(s) 431
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse1I ACTGG 3 cut(s) 110, 224, 353
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 431
BseGI GGATG 2 cut(s) 186, 427
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 3 cut(s) 110, 224, 353
BseSI GKGCMC 1 cut(s) 117
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 1 cut(s) 253
BshFI GGCC 1 cut(s) 85
BsiHKAI GWGCWC 1 cut(s) 117
BsiSI CCGG 3 cut(s) 65, 243, 404
BslI CCNNNNNNNGG 1 cut(s) 105
BsnI GGCC 1 cut(s) 85
Bsp1286I GDGCHC 1 cut(s) 117
Bsp143I GATC 2 cut(s) 94, 178
Bsp19I CCATGG 1 cut(s) 431
BspACI CCGC 1 cut(s) 42
BspANI GGCC 1 cut(s) 85
BspCNI CTCAG 1 cut(s) 45
BspFNI CGCG 1 cut(s) 253
BspPI GGATC 1 cut(s) 89
BsrI ACTGG 3 cut(s) 110, 224, 353
BssECI CCNNGG 1 cut(s) 431
BssMI GATC 2 cut(s) 94, 178
BssT1I CCWWGG 1 cut(s) 431
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 358
BstDEI CTNAG 1 cut(s) 53
BstDSI CCRYGG 1 cut(s) 431
BstF5I GGATG 2 cut(s) 186, 427
BstFNI CGCG 1 cut(s) 253
BstKTI GATC 2 cut(s) 97, 181
BstMBI GATC 2 cut(s) 94, 178
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 2 cut(s) 113, 290
BstSCI CCNGG 3 cut(s) 63, 85, 241
BstSLI GKGCMC 1 cut(s) 117
BstUI CGCG 1 cut(s) 253
BstV1I GCAGC 1 cut(s) 17
BsuRI GGCC 1 cut(s) 85
BtgI CCRYGG 1 cut(s) 431
BtrI CACGTC 1 cut(s) 118
BtsCI GGATG 2 cut(s) 186, 427
Cac8I GCNNGC 1 cut(s) 358
Cfr13I GGNCC 2 cut(s) 165, 336
CviAII CATG 6 cut(s) 110, 230, 272, 287, 432, 441
CviJI RGCY 5 cut(s) 8, 50, 85, 331, 421
CviKI_1 RGCY 5 cut(s) 8, 50, 85, 331, 421
DdeI CTNAG 1 cut(s) 53
DpnI GATC 2 cut(s) 96, 180
DpnII GATC 2 cut(s) 94, 178
Eco130I CCWWGG 1 cut(s) 431
Eco47I GGWCC 2 cut(s) 165, 336
EcoRII CCWGG 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 431
ErhI CCWWGG 1 cut(s) 431
FaeI CATG 6 cut(s) 113, 233, 275, 290, 435, 444
FatI CATG 6 cut(s) 109, 229, 271, 286, 431, 440
FauNDI CATATG 1 cut(s) 324
Fnu4HI GCNGC 2 cut(s) 6, 42
FokI GGATG 2 cut(s) 173, 414
Fsp4HI GCNGC 2 cut(s) 6, 42
GluI GCNGC 2 cut(s) 6, 42
HaeIII GGCC 1 cut(s) 85
HapII CCGG 3 cut(s) 65, 243, 404
Hin1II CATG 6 cut(s) 113, 233, 275, 290, 435, 444
HincII GTYRAC 2 cut(s) 124, 235
HindII GTYRAC 2 cut(s) 124, 235
HinfI GANTC 2 cut(s) 68, 409
HpaI GTTAAC 1 cut(s) 124
HpaII CCGG 3 cut(s) 65, 243, 404
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 5 cut(s) 115, 124, 137, 235, 320
Hpy188I TCNGA 2 cut(s) 169, 336
Hpy188III TCNNGA 3 cut(s) 92, 394, 413
Hpy8I GTNNAC 5 cut(s) 115, 124, 137, 235, 320
Hpy99I CGWCG 2 cut(s) 122, 179
HpyAV CCTTC 1 cut(s) 304
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 2 cut(s) 115, 275
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 6 cut(s) 113, 233, 275, 290, 435, 444
KspAI GTTAAC 1 cut(s) 124
Kzo9I GATC 2 cut(s) 94, 178
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 195
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 3 cut(s) 209, 344, 413
MalI GATC 2 cut(s) 96, 180
MboI GATC 2 cut(s) 94, 178
MboII GAAGA 1 cut(s) 52
MhlI GDGCHC 1 cut(s) 117
MluI ACGCGT 1 cut(s) 251
MlyI GAGTC 1 cut(s) 418
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 3 cut(s) 82, 182, 401
MseI TTAA 1 cut(s) 123
MslI CAYNNNNRTG 1 cut(s) 228
MspI CCGG 3 cut(s) 65, 243, 404
MspR9I CCNGG 3 cut(s) 65, 87, 243
MvaI CCWGG 1 cut(s) 87
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 1 cut(s) 47
NciI CCSGG 2 cut(s) 65, 243
NcoI CCATGG 1 cut(s) 431
NdeI CATATG 1 cut(s) 324
NdeII GATC 2 cut(s) 94, 178
NlaIII CATG 6 cut(s) 113, 233, 275, 290, 435, 444
NmuCI GTSAC 3 cut(s) 209, 344, 413
NspI RCATGY 2 cut(s) 113, 290
PfeI GAWTC 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 105
PfoI TCCNGGA 1 cut(s) 63
PkrI GCNGC 2 cut(s) 7, 43
PleI GAGTC 1 cut(s) 417
PpsI GAGTC 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 85
PspGI CCWGG 1 cut(s) 85
PspPI GGNCC 2 cut(s) 165, 336
RseI CAYNNNNRTG 1 cut(s) 228
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 2 cut(s) 6, 42
Sau3AI GATC 2 cut(s) 94, 178
Sau96I GGNCC 2 cut(s) 165, 336
SchI GAGTC 1 cut(s) 418
ScrFI CCNGG 3 cut(s) 65, 87, 243
SduI GDGCHC 1 cut(s) 117
SetI ASST 5 cut(s) 10, 120, 174, 393, 423
SfaNI GCATC 1 cut(s) 195
SinI GGWCC 2 cut(s) 165, 336
SmiMI CAYNNNNRTG 1 cut(s) 228
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
SsiI CCGC 1 cut(s) 42
StyD4I CCNGG 3 cut(s) 63, 85, 241
StyI CCWWGG 1 cut(s) 431
TaaI ACNGT 1 cut(s) 128
TaiI ACGT 1 cut(s) 120
TaqI TCGA 1 cut(s) 174
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TseFI GTSAC 3 cut(s) 209, 344, 413
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 3 cut(s) 209, 344, 413
TspDTI ATGAA 1 cut(s) 416
Van91I CCANNNNNTGG 1 cut(s) 105
VneI GTGCAC 1 cut(s) 113
VpaK11BI GGWCC 2 cut(s) 165, 336
XceI RCATGY 2 cut(s) 113, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.