Rh6AG296300

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000965
Physical Location & Seq
Reverse (-)
49403813 .. 49405132
1320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG296300.1

Sequence Viewer

Length: 582 bp
ATGAGCATTAGTATGAAGCACTCTATTGTTTTTTTAATCTCTTTGGCAATACTCTCAGCCTTGACTGGTTGCGGAAATGCTGCTGAGAACGATATCCACGTGCCGGGTGTCGACAGGTGGTACGTGCACGTCGTCAATGGACTCGGGGGAGGTAGATGGCTCTTCGTTCATTGCCAGTCCAAAGACGACGATCTCGGAACTCATGATCTAGCAACAGGGAAGGAGTTTTGGTGGACGTTCAAGACAAGTTATTTCGGGCGCACACTTTTCTGGTGCTACCTGCGGACCGACGAAGCCACCAACAAACAACATGCTACTTTTGATGCGTTTTGGCATGAGAAGCACCATTTTTGGCTTTTGGATCATTGTGGCGATGAAGAAAGGAATTGCATCTGGACTGCAAAGGATGATGGGATTTACTTGAAAATCAATTCTAAAAATGTTGATGACTTTGTTCATAAATGGGAACCAGTGAATACTCACATTTTCGAAGAAGAGGCCCCATTGGAAGGCCTTCTTGATGTTCTAGGAATCCCAGTGAGCAAGTCGACCAGAGGATTGGTACCCAGAAAGCAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

193

Amino Acids

22.17

Weight (kDa)

5.91

Isoelectric Point (pI)

34.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 42 - 155 2.9e-26 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 288
Acc65I GGTACC 1 cut(s) 562
AccB1I GGYRCC 1 cut(s) 562
AccI GTMKAC 2 cut(s) 111, 548
AciI CCGC 2 cut(s) 72, 283
AclWI GGATC 1 cut(s) 369
AcvI CACGTG 1 cut(s) 100
AfaI GTAC 2 cut(s) 122, 564
AfiI CCNNNNNNNGG 2 cut(s) 103, 509
AgsI TTSAA 2 cut(s) 241, 424
AjiI CACGTC 1 cut(s) 130
Alw21I GWGCWC 1 cut(s) 129
Alw44I GTGCAC 1 cut(s) 125
AlwI GGATC 1 cut(s) 369
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 2 cut(s) 498, 511
ApaLI GTGCAC 1 cut(s) 125
ApeKI GCWGC 1 cut(s) 80
Asp700I GAANNNNTTC 1 cut(s) 513
Asp718I GGTACC 1 cut(s) 562
AspLEI GCGC 1 cut(s) 261
AspS9I GGNCC 2 cut(s) 285, 499
AsuC2I CCSGG 1 cut(s) 105
AsuII TTCGAA 1 cut(s) 489
AvaI CYCGRG 1 cut(s) 143
AvaII GGWCC 1 cut(s) 285
BaeGI GKGCMC 1 cut(s) 129
BanI GGYRCC 1 cut(s) 562
BbrPI CACGTG 1 cut(s) 100
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 67
BccI CCATC 2 cut(s) 150, 404
BcnI CCSGG 1 cut(s) 105
BfaI CTAG 2 cut(s) 209, 527
BfuAI ACCTGC 1 cut(s) 288
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 1 cut(s) 285
BmeT110I CYCGRG 1 cut(s) 143
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 2 cut(s) 285, 499
BmiI GGNNCC 3 cut(s) 468, 501, 564
BmrFI CCNGG 1 cut(s) 105
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 2 cut(s) 313, 399
BmuI ACTGGG 1 cut(s) 530
Bpu14I TTCGAA 1 cut(s) 489
BpuMI CCSGG 1 cut(s) 105
BsaAI YACGTR 2 cut(s) 100, 124
BsaXI ACNNNNNCTCC 2 cut(s) 215, 245
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 509
Bse1I ACTGG 4 cut(s) 70, 175, 470, 536
Bse3DI GCAATG 1 cut(s) 169
BseGI GGATG 1 cut(s) 412
BseLI CCNNNNNNNGG 2 cut(s) 103, 509
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 2 cut(s) 69, 75
BseNI ACTGG 4 cut(s) 70, 175, 470, 536
BseSI GKGCMC 1 cut(s) 129
BseXI GCAGC 1 cut(s) 67
BshFI GGCC 2 cut(s) 500, 513
BshNI GGYRCC 1 cut(s) 562
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 1 cut(s) 104
BslI CCNNNNNNNGG 2 cut(s) 103, 509
BsnI GGCC 2 cut(s) 500, 513
BsoBI CYCGRG 1 cut(s) 143
Bsp119I TTCGAA 1 cut(s) 489
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 3 cut(s) 190, 205, 361
BspACI CCGC 2 cut(s) 72, 283
BspANI GGCC 2 cut(s) 500, 513
BspCNI CTCAG 2 cut(s) 68, 76
BspHI TCATGA 1 cut(s) 202
BspLI GGNNCC 3 cut(s) 468, 501, 564
BspMI ACCTGC 1 cut(s) 288
BspPI GGATC 1 cut(s) 369
BspQI GCTCTTC 1 cut(s) 167
BspT104I TTCGAA 1 cut(s) 489
BspT107I GGYRCC 1 cut(s) 562
BsrDI GCAATG 1 cut(s) 169
BsrI ACTGG 4 cut(s) 70, 175, 470, 536
BssMI GATC 3 cut(s) 190, 205, 361
Bst6I CTCTTC 2 cut(s) 167, 489
BstBAI YACGTR 2 cut(s) 100, 124
BstBI TTCGAA 1 cut(s) 489
BstDEI CTNAG 2 cut(s) 55, 84
BstF5I GGATG 1 cut(s) 412
BstHHI GCGC 1 cut(s) 261
BstKTI GATC 3 cut(s) 193, 208, 364
BstMBI GATC 3 cut(s) 190, 205, 361
BstMWI GCNNNNNNNGC 1 cut(s) 340
BstNSI RCATGY 1 cut(s) 314
BstSCI CCNGG 1 cut(s) 103
BstSLI GKGCMC 1 cut(s) 129
BstV1I GCAGC 1 cut(s) 67
BstXI CCANNNNNNTGG 1 cut(s) 559
BsuRI GGCC 2 cut(s) 500, 513
BtgZI GCGATG 1 cut(s) 387
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 1 cut(s) 412
BtsIMutI CAGTG 2 cut(s) 477, 543
BveI ACCTGC 1 cut(s) 288
CciI TCATGA 1 cut(s) 202
CfoI GCGC 1 cut(s) 261
Cfr13I GGNCC 2 cut(s) 285, 499
CpoI CGGWCCG 1 cut(s) 285
Csp6I GTAC 2 cut(s) 121, 563
CspI CGGWCCG 1 cut(s) 285
CviAII CATG 3 cut(s) 203, 311, 335
CviJI RGCY 6 cut(s) 59, 160, 296, 355, 500, 513
CviKI_1 RGCY 6 cut(s) 59, 160, 296, 355, 500, 513
CviQI GTAC 2 cut(s) 121, 563
DdeI CTNAG 2 cut(s) 55, 84
DpnI GATC 3 cut(s) 192, 207, 363
DpnII GATC 3 cut(s) 190, 205, 361
Eam1104I CTCTTC 2 cut(s) 167, 489
EarI CTCTTC 2 cut(s) 167, 489
Eco147I AGGCCT 1 cut(s) 513
Eco32I GATATC 1 cut(s) 94
Eco47I GGWCC 1 cut(s) 285
Eco72I CACGTG 1 cut(s) 100
Eco88I CYCGRG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 499
EcoRV GATATC 1 cut(s) 94
FaeI CATG 3 cut(s) 206, 314, 338
FaiI YATR 5 cut(s) 14, 204, 312, 336, 459
FalI AAGNNNNNCTT 2 cut(s) 501, 533
FatI CATG 3 cut(s) 202, 310, 334
FblI GTMKAC 2 cut(s) 111, 548
Fnu4HI GCNGC 1 cut(s) 81
FokI GGATG 1 cut(s) 419
Fsp4HI GCNGC 1 cut(s) 81
FspBI CTAG 2 cut(s) 209, 527
GlaI GCGC 1 cut(s) 260
GluI GCNGC 1 cut(s) 81
HaeIII GGCC 2 cut(s) 500, 513
HapII CCGG 1 cut(s) 104
HhaI GCGC 1 cut(s) 261
Hin1II CATG 3 cut(s) 206, 314, 338
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HincII GTYRAC 2 cut(s) 112, 549
HindII GTYRAC 2 cut(s) 112, 549
HinfI GANTC 2 cut(s) 141, 531
HpaII CCGG 1 cut(s) 104
Hpy166II GTNNAC 4 cut(s) 112, 127, 234, 549
Hpy188I TCNGA 1 cut(s) 197
Hpy188III TCNNGA 4 cut(s) 203, 241, 394, 518
Hpy8I GTNNAC 4 cut(s) 112, 127, 234, 549
Hpy99I CGWCG 3 cut(s) 134, 191, 293
HpyAV CCTTC 3 cut(s) 214, 503, 524
HpyCH4IV ACGT 4 cut(s) 99, 123, 129, 236
HpyCH4V TGCA 3 cut(s) 127, 390, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 340
HpyF3I CTNAG 2 cut(s) 55, 84
HpySE526I ACGT 4 cut(s) 99, 123, 129, 236
Hsp92II CATG 3 cut(s) 206, 314, 338
HspAI GCGC 1 cut(s) 259
KpnI GGTACC 1 cut(s) 566
Kzo9I GATC 3 cut(s) 190, 205, 361
LguI GCTCTTC 1 cut(s) 167
Lsp1109I GCAGC 1 cut(s) 67
LweI GCATC 2 cut(s) 313, 399
MaeI CTAG 2 cut(s) 209, 527
MaeII ACGT 4 cut(s) 99, 123, 129, 236
MalI GATC 3 cut(s) 192, 207, 363
MboI GATC 3 cut(s) 190, 205, 361
MboII GAAGA 4 cut(s) 154, 389, 503, 506
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 3 cut(s) 385, 430, 577
MlyI GAGTC 1 cut(s) 135
MnlI CCTC 3 cut(s) 143, 490, 548
MroXI GAANNNNTTC 1 cut(s) 513
MseI TTAA 1 cut(s) 35
MslI CAYNNNNRTG 1 cut(s) 11
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 1 cut(s) 105
MwoI GCNNNNNNNGC 1 cut(s) 340
NciI CCSGG 1 cut(s) 105
NdeII GATC 3 cut(s) 190, 205, 361
NlaIII CATG 3 cut(s) 206, 314, 338
NlaIV GGNNCC 3 cut(s) 468, 501, 564
NspI RCATGY 1 cut(s) 314
NspV TTCGAA 1 cut(s) 489
PagI TCATGA 1 cut(s) 202
PceI AGGCCT 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 167
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 129
PdmI GAANNNNTTC 1 cut(s) 513
PfeI GAWTC 1 cut(s) 531
PkrI GCNGC 1 cut(s) 82
PleI GAGTC 1 cut(s) 135
PmaCI CACGTG 1 cut(s) 100
PmlI CACGTG 1 cut(s) 100
PpsI GAGTC 1 cut(s) 135
Ppu21I YACGTR 2 cut(s) 100, 124
PspCI CACGTG 1 cut(s) 100
PspN4I GGNNCC 3 cut(s) 468, 501, 564
PspPI GGNCC 2 cut(s) 285, 499
RsaI GTAC 2 cut(s) 122, 564
RsaNI GTAC 2 cut(s) 121, 563
RseI CAYNNNNRTG 1 cut(s) 11
Rsr2I CGGWCCG 1 cut(s) 285
RsrII CGGWCCG 1 cut(s) 285
SalI GTCGAC 2 cut(s) 110, 547
SapI GCTCTTC 1 cut(s) 167
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 81
Sau3AI GATC 3 cut(s) 190, 205, 361
Sau96I GGNCC 2 cut(s) 285, 499
SchI GAGTC 1 cut(s) 135
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 1 cut(s) 129
SetI ASST 7 cut(s) 102, 119, 126, 132, 154, 239, 282
SfaNI GCATC 2 cut(s) 313, 399
SfuI TTCGAA 1 cut(s) 489
SinI GGWCC 1 cut(s) 285
SmiMI CAYNNNNRTG 1 cut(s) 11
Sse9I AATT 3 cut(s) 385, 430, 577
SseBI AGGCCT 1 cut(s) 513
SsiI CCGC 2 cut(s) 72, 283
SspMI CTAG 2 cut(s) 209, 527
StuI AGGCCT 1 cut(s) 513
StyD4I CCNGG 1 cut(s) 103
TaiI ACGT 4 cut(s) 102, 126, 132, 239
TaqI TCGA 3 cut(s) 111, 489, 548
TaqII GACCGA 1 cut(s) 302
TasI AATT 3 cut(s) 385, 430, 577
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 2 cut(s) 477, 543
TseI GCWGC 1 cut(s) 80
TspDTI ATGAA 4 cut(s) 29, 158, 390, 446
TspRI CASTG 2 cut(s) 477, 543
VneI GTGCAC 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 285
XceI RCATGY 1 cut(s) 314
XmiI GTMKAC 2 cut(s) 111, 548
XmnI GAANNNNTTC 1 cut(s) 513
XspI CTAG 2 cut(s) 209, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.