FvH4_2g20680

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000965
Physical Location & Seq
Reverse (-)
17297574 .. 17298263
690 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20680.t1

Sequence Viewer

Length: 486 bp
ATGAGCATTGGTATGAAGAACTCTATTGTTTTGTTCATCTTCGTGGCAATATTCACGGCCTTGAATGACCGTGGAAATGCTGCTCCCCCGAGCAAATACCACCCAGCGGGTGTCGACAGGTGGTACATCCACGTCGTCAATAAACTCGGGGGAGGTAGATGGCTCGCCGTTCATTGCAAGTCCAAAGATGACGACCTCGGGAATCATGATCTAGCACCAGGGCAGGACTATTGGTTTACGTTCAAGTCAAACTATTGGGGAACCACGCTTTTCTGGTGCACCCTGCGCACCGAAAAAGAAGACCCTGTAAAACAACATGTTGGTTTGAAAGTGTTTTGGCAGGAATGGCTACATGTTTGGCTCACGTATCATTGTGGCCAAAAAGAAAGGAAATGCTTCTGGATTGCAAAAGATGATGGGATTTACCTGAGAGTCAATCCTGAAAATCGTGATGAATTTGTTGTTAAATGGGAAACGGGGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

162

Amino Acids

18.94

Weight (kDa)

8.32

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 43 - 157 1.6e-27 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 287
AccI GTMKAC 1 cut(s) 114
AciI CCGC 1 cut(s) 107
AcoI YGGCCR 1 cut(s) 376
AcsI RAATTY 1 cut(s) 455
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 106
AflIII ACRYGT 2 cut(s) 316, 352
AgsI TTSAA 3 cut(s) 64, 244, 328
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 1 cut(s) 217
Alw21I GWGCWC 1 cut(s) 281
Alw44I GTGCAC 1 cut(s) 277
Ama87I CYCGRG 3 cut(s) 88, 146, 197
AoxI GGCC 2 cut(s) 57, 376
ApaLI GTGCAC 1 cut(s) 277
ApeKI GCWGC 1 cut(s) 80
ApoI RAATTY 1 cut(s) 455
Asp700I GAANNNNTTC 1 cut(s) 395
AspLEI GCGC 1 cut(s) 288
AvaI CYCGRG 3 cut(s) 88, 146, 197
BaeGI GKGCMC 1 cut(s) 281
BalI TGGCCA 1 cut(s) 378
BbsI GAAGAC 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 281
BbvI GCAGC 1 cut(s) 67
BccI CCATC 2 cut(s) 153, 410
BceAI ACGGC 2 cut(s) 72, 152
BciT130I CCWGG 1 cut(s) 219
BfaI CTAG 1 cut(s) 212
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
Bme1390I CCNGG 1 cut(s) 219
BmeT110I CYCGRG 3 cut(s) 88, 146, 197
BmgBI CACGTC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 262
BmrFI CCNGG 1 cut(s) 219
BpiI GAAGAC 1 cut(s) 306
BsaAI YACGTR 1 cut(s) 366
BsaJI CCNNGG 3 cut(s) 70, 196, 218
Bsc4I CCNNNNNNNGG 1 cut(s) 106
Bse3DI GCAATG 1 cut(s) 172
BseBI CCWGG 1 cut(s) 219
BseDI CCNNGG 3 cut(s) 70, 196, 218
BseGI GGATG 1 cut(s) 126
BseLI CCNNNNNNNGG 1 cut(s) 106
BseMI GCAATG 1 cut(s) 172
BseMII CTCAG 1 cut(s) 419
BseSI GKGCMC 1 cut(s) 281
BseXI GCAGC 1 cut(s) 67
BseYI CCCAGC 1 cut(s) 103
BshFI GGCC 2 cut(s) 59, 378
BsiHKAI GWGCWC 1 cut(s) 281
BsiHKCI CYCGRG 3 cut(s) 88, 146, 197
BslI CCNNNNNNNGG 1 cut(s) 106
BsnI GGCC 2 cut(s) 59, 378
BsoBI CYCGRG 3 cut(s) 88, 146, 197
Bsp1286I GDGCHC 1 cut(s) 281
Bsp143I GATC 1 cut(s) 208
BspACI CCGC 1 cut(s) 107
BspANI GGCC 2 cut(s) 59, 378
BspCNI CTCAG 1 cut(s) 420
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 1 cut(s) 262
BsrDI GCAATG 1 cut(s) 172
BssECI CCNNGG 3 cut(s) 70, 196, 218
BssMI GATC 1 cut(s) 208
Bst2UI CCWGG 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 71
BstBAI YACGTR 1 cut(s) 366
BstC8I GCNNGC 1 cut(s) 165
BstDEI CTNAG 1 cut(s) 428
BstDSI CCRYGG 1 cut(s) 70
BstF5I GGATG 1 cut(s) 126
BstHHI GCGC 1 cut(s) 288
BstKTI GATC 1 cut(s) 211
BstMBI GATC 1 cut(s) 208
BstMWI GCNNNNNNNGC 2 cut(s) 285, 346
BstNI CCWGG 1 cut(s) 219
BstNSI RCATGY 2 cut(s) 320, 356
BstSCI CCNGG 1 cut(s) 217
BstSLI GKGCMC 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 67
BstV2I GAAGAC 1 cut(s) 306
BsuRI GGCC 2 cut(s) 59, 378
BtgI CCRYGG 1 cut(s) 70
BtrI CACGTC 1 cut(s) 133
BtsCI GGATG 1 cut(s) 126
Cac8I GCNNGC 1 cut(s) 165
CciI TCATGA 1 cut(s) 205
CfoI GCGC 1 cut(s) 288
Csp6I GTAC 1 cut(s) 124
CviAII CATG 3 cut(s) 206, 317, 353
CviJI RGCY 5 cut(s) 59, 163, 349, 361, 378
CviKI_1 RGCY 5 cut(s) 59, 163, 349, 361, 378
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 1 cut(s) 428
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 376
Eco88I CYCGRG 3 cut(s) 88, 146, 197
EcoRII CCWGG 1 cut(s) 217
FaeI CATG 3 cut(s) 209, 320, 356
FaiI YATR 4 cut(s) 14, 207, 318, 354
FatI CATG 3 cut(s) 205, 316, 352
FauI CCCGC 1 cut(s) 100
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 1 cut(s) 81
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 81
FspBI CTAG 1 cut(s) 212
FspI TGCGCA 1 cut(s) 287
GlaI GCGC 1 cut(s) 287
GluI GCNGC 1 cut(s) 81
GsaI CCCAGC 1 cut(s) 107
HaeIII GGCC 2 cut(s) 59, 378
HhaI GCGC 1 cut(s) 288
Hin1II CATG 3 cut(s) 209, 320, 356
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 2 cut(s) 202, 432
Hpy166II GTNNAC 3 cut(s) 115, 237, 279
Hpy188III TCNNGA 5 cut(s) 199, 206, 400, 440, 449
Hpy8I GTNNAC 3 cut(s) 115, 237, 279
Hpy99I CGWCG 1 cut(s) 137
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4IV ACGT 3 cut(s) 132, 239, 365
HpyCH4V TGCA 3 cut(s) 177, 279, 407
HpyF10VI GCNNNNNNNGC 2 cut(s) 285, 346
HpyF3I CTNAG 1 cut(s) 428
HpySE526I ACGT 3 cut(s) 132, 239, 365
Hsp92II CATG 3 cut(s) 209, 320, 356
HspAI GCGC 1 cut(s) 286
Kzo9I GATC 1 cut(s) 208
LmnI GCTCC 1 cut(s) 88
Lsp1109I GCAGC 1 cut(s) 67
MaeI CTAG 1 cut(s) 212
MaeII ACGT 3 cut(s) 132, 239, 365
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 3 cut(s) 28, 31, 311
MhlI GDGCHC 1 cut(s) 281
MlsI TGGCCA 1 cut(s) 378
MluCI AATT 1 cut(s) 455
MluNI TGGCCA 1 cut(s) 378
MlyI GAGTC 1 cut(s) 441
MnlI CCTC 2 cut(s) 146, 206
Mox20I TGGCCA 1 cut(s) 378
MroXI GAANNNNTTC 1 cut(s) 395
MscI TGGCCA 1 cut(s) 378
MseI TTAA 1 cut(s) 465
MslI CAYNNNNRTG 2 cut(s) 11, 41
Msp20I TGGCCA 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 107
MspR9I CCNGG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 219
MwoI GCNNNNNNNGC 2 cut(s) 285, 346
NdeII GATC 1 cut(s) 208
NlaIII CATG 3 cut(s) 209, 320, 356
NlaIV GGNNCC 1 cut(s) 262
NsbI TGCGCA 1 cut(s) 287
NspI RCATGY 2 cut(s) 320, 356
PagI TCATGA 1 cut(s) 205
PciI ACATGT 2 cut(s) 316, 352
PdmI GAANNNNTTC 1 cut(s) 395
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 1 cut(s) 82
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
Ppu21I YACGTR 1 cut(s) 366
PscI ACATGT 2 cut(s) 316, 352
Psp6I CCWGG 1 cut(s) 217
PspFI CCCAGC 1 cut(s) 103
PspGI CCWGG 1 cut(s) 217
PspN4I GGNNCC 1 cut(s) 262
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
RseI CAYNNNNRTG 2 cut(s) 11, 41
SalI GTCGAC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 1 cut(s) 81
Sau3AI GATC 1 cut(s) 208
SchI GAGTC 1 cut(s) 441
ScrFI CCNGG 1 cut(s) 219
SduI GDGCHC 1 cut(s) 281
SetI ASST 7 cut(s) 122, 135, 157, 198, 242, 368, 429
SmiMI CAYNNNNRTG 2 cut(s) 11, 41
Sse9I AATT 1 cut(s) 455
SsiI CCGC 1 cut(s) 107
SspI AATATT 1 cut(s) 51
SspMI CTAG 1 cut(s) 212
StyD4I CCNGG 1 cut(s) 217
TaaI ACNGT 1 cut(s) 71
TaiI ACGT 3 cut(s) 135, 242, 368
TaqI TCGA 1 cut(s) 114
TasI AATT 1 cut(s) 455
TfiI GAWTC 1 cut(s) 202
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TseI GCWGC 1 cut(s) 80
TspDTI ATGAA 4 cut(s) 25, 29, 161, 468
VneI GTGCAC 1 cut(s) 277
XapI RAATTY 1 cut(s) 455
XceI RCATGY 2 cut(s) 320, 356
XmiI GTMKAC 1 cut(s) 114
XmnI GAANNNNTTC 1 cut(s) 395
XspI CTAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.