Rh6AG296400

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000965
Physical Location & Seq
Reverse (-)
49415378 .. 49430282
14905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG296400.1

Sequence Viewer

Length: 1665 bp
ATGAGCATTAGTATGAAGCACTCTATTGTTTTGTTAATCTCTTTGGCAATACTCTCAGCCTTGACTGGTCGTGGAAATGCTGCCGAAAACAATATCCACGTGCCGGGTGTCGACAGGTGGTACGTGCACGTCGTCAATGGACTCGGGGGAGGTAGATGGCTCTTCGTTCATTGCCAGTCCAAAGACGACGATCTCGGAACTCATGATCTAGCAACAGGGAAGGAGTTTTGGTGGACGTTCAAGACAAGCTATTTCGGGCGCACACTTTTCTGGTGCTACCTGCGAACCGACGAAGCCACCAACAAACAACATGCTACTTTTGATGCGTTTTGGCATGAGAAACACCATTTTTGGCTTTTGGATCATTGTGGCGATGAAGAAAGGAATTGCATCTGGACTGCAAAAGATGATGGGATTTACTTGAAAATCAATTCTAAAAATGTTGATGACTTTGTTCATAAATGGGAACCAGAGCACAACATCATGAAAGCAGTTCAGGAGACTACTCTGCGTCTCCTCCTCCTCCATATTTTCTTGCTCTTTTTGCTTCCATTGGAGGCCACGTCATCACAAAGAATACAAGCTGAAGCTCTCCTAAAATGGAAGAACAGCTTTACTTCTTCACCACCTTCTCTGAATTCATGGTCGCTCACAAACCTAAACAACCTGTGCAACTGGTCTGGCATTGTCTGCAACCACGATACCAAAACAGTTTCTGAAATTCACCTTGAAAACTTCAACATCACTGCAGCACTAACGCAGTTCAACTTCGACAAGTTTCTCAATCTCACCCATTTCAACCTCAATGGCAACAATTTCTCAGGGCCTATACCATCTGCCATTGGCAACCTCACCAAGCTCACAACTTTGGATTTGGGCAACAATCATTTTGAACAGGAAGTTCCTGTGGAGATGGGGAAGCTAACCCAGGTTGAGTATTTTAGTCTTTACAGCAACAGTCTCACTGGAGCCATTCCATATCAGCTGGACAATCTCAAAAAGCTCTCCGGGCCTATTCCTATGGCATTCTGGAATCTCACTAATCTTCAAAGTGTACAGCTTTTCTCCAATAACCTCAGTGGCACAATCCCCCCAGAGATTGGGAACATGACTTCATTGTCTGTCTTTGATGTCAACACAAACCAATTTGAGGGGGAGTTGCCAGAGACCATTTCTCTCCTCACTAACCTTGTGGGCTTTTCTGTATTCACCAATAAGTTGTCCGGAAATATTCCTAGTGATTTTGGGAATGGGGAAATTCCAGTTGGTATTGGGAATCTAAGCTTGTTATACACTCTCAATCTCAGCAGGAACCACTTCACAGGAACCATCCCGGAGATCCACCAATTGACTAAGCTCCAGACTCTCGATTTGTCTGATAACAATTTTACAGGGGAATTACCAATAGAGCCTGGAACTTTTGAGAGCTTAATAACCTTGAACATAAGCCGCAACAAATTATCAGGAGGAATACCAGAGGAGATTGGTAATCTACACCTGCGGGGCAGGTACTTGGACCTAAGCAGCAATTCATTCTCGGGATCCCTACCATTAAACCTTGCCAAGCTTACAATGCTGGAGATTCTTAATGTCTCACATAACCAGCTCTCAGGGAATATCCCATCAACATATTCCAGAATGATGTTTATACTCGCCCATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

554

Amino Acids

62.22

Weight (kDa)

5.79

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 42 - 155 2.8e-25 Plant self-incompatibility protein S1
LRRNT_2 PF08263 194 - 232 6.1e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 245 - 335 4.2e-06 Leucine-rich repeat region
LRR_14 PF23598 424 - 499 9.2e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1506
AasI GACNNNNNNGTC 1 cut(s) 1117
Acc36I ACCTGC 3 cut(s) 288, 1497, 1506
AccB7I CCANNNNNTGG 1 cut(s) 1100
AccI GTMKAC 1 cut(s) 111
AccIII TCCGGA 1 cut(s) 1223
AciI CCGC 2 cut(s) 1450, 1501
AclWI GGATC 4 cut(s) 369, 1333, 1536, 1549
AcsI RAATTY 3 cut(s) 637, 720, 1257
AcuI CTGAAG 1 cut(s) 606
AcvI CACGTG 1 cut(s) 100
AfaI GTAC 3 cut(s) 122, 1056, 1511
AfiI CCNNNNNNNGG 3 cut(s) 103, 1100, 1150
AgsI TTSAA 9 cut(s) 241, 424, 731, 739, 766, 799, 893, 1049, 1441
AjiI CACGTC 2 cut(s) 130, 564
AjnI CCWGG 2 cut(s) 927, 1411
AjuI GAANNNNNNNTTGG 2 cut(s) 1248, 1280
Alw21I GWGCWC 2 cut(s) 129, 477
Alw26I GTCTC 5 cut(s) 494, 518, 965, 1160, 1597
Alw44I GTGCAC 1 cut(s) 125
AlwI GGATC 4 cut(s) 369, 1333, 1536, 1549
AlwNI CAGNNNCTG 1 cut(s) 716
Ama87I CYCGRG 2 cut(s) 143, 1537
Aor13HI TCCGGA 1 cut(s) 1223
AoxI GGCC 3 cut(s) 558, 824, 1010
ApaLI GTGCAC 1 cut(s) 125
ApeKI GCWGC 3 cut(s) 80, 749, 1524
ApoI RAATTY 3 cut(s) 637, 720, 1257
ArsI GACNNNNNNTTYG 2 cut(s) 1354, 1386
Asp700I GAANNNNTTC 1 cut(s) 1316
AspLEI GCGC 1 cut(s) 261
AspS9I GGNCC 3 cut(s) 824, 1010, 1516
AsuC2I CCSGG 3 cut(s) 105, 1009, 1334
AsuHPI GGTGA 5 cut(s) 615, 716, 781, 844, 1201
AvaI CYCGRG 2 cut(s) 143, 1537
AvaII GGWCC 1 cut(s) 1516
BaeGI GKGCMC 1 cut(s) 129
BamHI GGATCC 1 cut(s) 1541
BbrPI CACGTG 1 cut(s) 100
Bbv12I GWGCWC 2 cut(s) 129, 477
BbvI GCAGC 3 cut(s) 67, 761, 1536
BccI CCATC 6 cut(s) 150, 404, 841, 907, 1337, 1630
BciT130I CCWGG 2 cut(s) 929, 1413
BcnI CCSGG 3 cut(s) 105, 1009, 1334
BcoDI GTCTC 5 cut(s) 494, 518, 965, 1160, 1597
BfaI CTAG 2 cut(s) 209, 1236
BfmI CTRYAG 1 cut(s) 747
BfuAI ACCTGC 3 cut(s) 288, 1497, 1506
BisI GCNGC 4 cut(s) 81, 750, 1450, 1525
BlsI GCNGC 4 cut(s) 82, 751, 1451, 1526
Bme1390I CCNGG 5 cut(s) 105, 929, 1009, 1334, 1413
Bme18I GGWCC 1 cut(s) 1516
BmeT110I CYCGRG 2 cut(s) 143, 1537
BmgBI CACGTC 2 cut(s) 130, 564
BmgT120I GGNCC 3 cut(s) 824, 1010, 1516
BmiI GGNNCC 5 cut(s) 468, 970, 1313, 1327, 1543
BmrFI CCNGG 5 cut(s) 105, 929, 1009, 1334, 1413
BmsI GCATC 2 cut(s) 313, 399
BpmI CTGGAG 3 cut(s) 987, 1343, 1598
Bpu10I CCTNAGC 1 cut(s) 1520
BpuMI CCSGG 3 cut(s) 105, 1009, 1334
BsaAI YACGTR 2 cut(s) 100, 124
BsaI GGTCTC 1 cut(s) 1160
BsaJI CCNNGG 1 cut(s) 927
BsaWI WCCGGW 1 cut(s) 1223
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 548, 578
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 1100, 1150
Bse1I ACTGG 5 cut(s) 70, 175, 680, 970, 1262
Bse3DI GCAATG 1 cut(s) 169
BseAI TCCGGA 1 cut(s) 1223
BseBI CCWGG 2 cut(s) 929, 1413
BseDI CCNNGG 1 cut(s) 927
BseGI GGATG 1 cut(s) 1329
BseLI CCNNNNNNNGG 3 cut(s) 103, 1100, 1150
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 5 cut(s) 69, 834, 1090, 1318, 1623
BseNI ACTGG 5 cut(s) 70, 175, 680, 970, 1262
BseRI GAGGAG 5 cut(s) 506, 509, 512, 1169, 1493
BseSI GKGCMC 1 cut(s) 129
BseXI GCAGC 3 cut(s) 67, 761, 1536
BshFI GGCC 3 cut(s) 560, 826, 1012
BsiHKAI GWGCWC 2 cut(s) 129, 477
BsiHKCI CYCGRG 2 cut(s) 143, 1537
BsiSI CCGG 4 cut(s) 104, 1008, 1224, 1334
BslI CCNNNNNNNGG 3 cut(s) 103, 1100, 1150
BsmAI GTCTC 5 cut(s) 494, 518, 965, 1160, 1597
BsmBI CGTCTC 1 cut(s) 518
BsmI GAATGC 1 cut(s) 1025
BsnI GGCC 3 cut(s) 560, 826, 1012
Bso31I GGTCTC 1 cut(s) 1160
BsoBI CYCGRG 2 cut(s) 143, 1537
Bsp1286I GDGCHC 2 cut(s) 129, 477
Bsp13I TCCGGA 1 cut(s) 1223
Bsp1407I TGTACA 1 cut(s) 1054
Bsp143I GATC 5 cut(s) 190, 205, 361, 1338, 1541
BspACI CCGC 2 cut(s) 1450, 1501
BspANI GGCC 3 cut(s) 560, 826, 1012
BspCNI CTCAG 5 cut(s) 68, 833, 1089, 1317, 1622
BspEI TCCGGA 1 cut(s) 1223
BspHI TCATGA 2 cut(s) 202, 483
BspLI GGNNCC 5 cut(s) 468, 970, 1313, 1327, 1543
BspMAI CTGCAG 1 cut(s) 751
BspMI ACCTGC 3 cut(s) 288, 1497, 1506
BspPI GGATC 4 cut(s) 369, 1333, 1536, 1549
BspQI GCTCTTC 1 cut(s) 167
BspTNI GGTCTC 1 cut(s) 1160
BsrDI GCAATG 1 cut(s) 169
BsrGI TGTACA 1 cut(s) 1054
BsrI ACTGG 5 cut(s) 70, 175, 680, 970, 1262
BssECI CCNNGG 1 cut(s) 927
BssMI GATC 5 cut(s) 190, 205, 361, 1338, 1541
Bst2UI CCWGG 2 cut(s) 929, 1413
Bst4CI ACNGT 2 cut(s) 712, 959
Bst6I CTCTTC 1 cut(s) 167
BstAPI GCANNNNNTGC 1 cut(s) 690
BstAUI TGTACA 1 cut(s) 1054
BstBAI YACGTR 2 cut(s) 100, 124
BstDEI CTNAG 8 cut(s) 55, 820, 1076, 1280, 1304, 1353, 1520, 1609
BstF5I GGATG 1 cut(s) 1329
BstHHI GCGC 1 cut(s) 261
BstKTI GATC 5 cut(s) 193, 208, 364, 1341, 1544
BstMAI GTCTC 5 cut(s) 494, 518, 965, 1160, 1597
BstMBI GATC 5 cut(s) 190, 205, 361, 1338, 1541
BstMWI GCNNNNNNNGC 4 cut(s) 544, 690, 1009, 1573
BstNI CCWGG 2 cut(s) 929, 1413
BstNSI RCATGY 1 cut(s) 314
BstSCI CCNGG 5 cut(s) 103, 927, 1007, 1332, 1411
BstSFI CTRYAG 1 cut(s) 747
BstSLI GKGCMC 1 cut(s) 129
BstV1I GCAGC 3 cut(s) 67, 761, 1536
BstX2I RGATCY 2 cut(s) 1338, 1541
BstYI RGATCY 2 cut(s) 1338, 1541
BsuRI GGCC 3 cut(s) 560, 826, 1012
BtgZI GCGATG 1 cut(s) 387
BtrI CACGTC 2 cut(s) 130, 564
BtsCI GGATG 1 cut(s) 1329
BtsI GCAGTG 1 cut(s) 744
BtsIMutI CAGTG 3 cut(s) 744, 963, 1084
BveI ACCTGC 3 cut(s) 288, 1497, 1506
CaiI CAGNNNCTG 1 cut(s) 716
CciI TCATGA 2 cut(s) 202, 483
CfoI GCGC 1 cut(s) 261
Cfr13I GGNCC 3 cut(s) 824, 1010, 1516
CseI GACGC 1 cut(s) 500
Csp6I GTAC 3 cut(s) 121, 1055, 1510
CviAII CATG 6 cut(s) 203, 311, 335, 484, 642, 1108
CviQI GTAC 3 cut(s) 121, 1055, 1510
DdeI CTNAG 8 cut(s) 55, 820, 1076, 1280, 1304, 1353, 1520, 1609
DpnI GATC 5 cut(s) 192, 207, 363, 1340, 1543
DpnII GATC 5 cut(s) 190, 205, 361, 1338, 1541
DrdI GACNNNNNNGTC 1 cut(s) 1117
DseDI GACNNNNNNGTC 1 cut(s) 1117
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
Eco31I GGTCTC 1 cut(s) 1160
Eco47I GGWCC 1 cut(s) 1516
Eco57I CTGAAG 1 cut(s) 606
Eco72I CACGTG 1 cut(s) 100
Eco88I CYCGRG 2 cut(s) 143, 1537
EcoO109I RGGNCCY 1 cut(s) 824
EcoRI GAATTC 1 cut(s) 637
EcoRII CCWGG 2 cut(s) 927, 1411
Esp3I CGTCTC 1 cut(s) 518
FaeI CATG 6 cut(s) 206, 314, 338, 487, 645, 1111
FalI AAGNNNNNCTT 2 cut(s) 596, 628
FatI CATG 6 cut(s) 202, 310, 334, 483, 641, 1107
FauI CCCGC 1 cut(s) 1494
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 4 cut(s) 81, 750, 1450, 1525
FokI GGATG 1 cut(s) 1316
Fsp4HI GCNGC 4 cut(s) 81, 750, 1450, 1525
FspBI CTAG 2 cut(s) 209, 1236
GlaI GCGC 1 cut(s) 260
GluI GCNGC 4 cut(s) 81, 750, 1450, 1525
GsuI CTGGAG 3 cut(s) 987, 1343, 1598
HaeIII GGCC 3 cut(s) 560, 826, 1012
HapII CCGG 4 cut(s) 104, 1008, 1224, 1334
HgaI GACGC 1 cut(s) 500
HhaI GCGC 1 cut(s) 261
Hin1II CATG 6 cut(s) 206, 314, 338, 487, 645, 1111
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HincII GTYRAC 2 cut(s) 112, 1135
HindII GTYRAC 2 cut(s) 112, 1135
HindIII AAGCTT 2 cut(s) 1282, 1565
HinfI GANTC 5 cut(s) 141, 1033, 1276, 1363, 1582
HpaII CCGG 4 cut(s) 104, 1008, 1224, 1334
HphI GGTGA 5 cut(s) 615, 716, 781, 844, 1201
Hpy166II GTNNAC 5 cut(s) 112, 127, 234, 1055, 1135
Hpy188I TCNGA 4 cut(s) 197, 636, 718, 1378
Hpy8I GTNNAC 5 cut(s) 112, 127, 234, 1055, 1135
Hpy99I CGWCG 3 cut(s) 134, 191, 293
HpyAV CCTTC 2 cut(s) 214, 639
HpyCH4III ACNGT 2 cut(s) 712, 959
HpyCH4IV ACGT 5 cut(s) 99, 123, 129, 236, 563
HpyCH4V TGCA 6 cut(s) 127, 390, 401, 672, 693, 749
HpyF10VI GCNNNNNNNGC 4 cut(s) 544, 690, 1009, 1573
HpyF3I CTNAG 8 cut(s) 55, 820, 1076, 1280, 1304, 1353, 1520, 1609
HpySE526I ACGT 5 cut(s) 99, 123, 129, 236, 563
Hsp92II CATG 6 cut(s) 206, 314, 338, 487, 645, 1111
HspAI GCGC 1 cut(s) 259
Kpn2I TCCGGA 1 cut(s) 1223
Kzo9I GATC 5 cut(s) 190, 205, 361, 1338, 1541
LguI GCTCTTC 1 cut(s) 167
LmnI GCTCC 2 cut(s) 968, 1362
Lsp1109I GCAGC 3 cut(s) 67, 761, 1536
LweI GCATC 2 cut(s) 313, 399
MaeI CTAG 2 cut(s) 209, 1236
MaeII ACGT 5 cut(s) 99, 123, 129, 236, 563
MalI GATC 5 cut(s) 192, 207, 363, 1340, 1543
MboI GATC 5 cut(s) 190, 205, 361, 1338, 1541
MboII GAAGA 5 cut(s) 154, 389, 612, 616, 1037
MfeI CAATTG 1 cut(s) 1346
MflI RGATCY 2 cut(s) 1338, 1541
MhlI GDGCHC 2 cut(s) 129, 477
MlyI GAGTC 2 cut(s) 135, 1357
MroI TCCGGA 1 cut(s) 1223
MroXI GAANNNNTTC 1 cut(s) 1316
MseI TTAA 5 cut(s) 35, 1430, 1553, 1587, 1663
MslI CAYNNNNRTG 1 cut(s) 11
MspA1I CMGCKG 1 cut(s) 985
MspI CCGG 4 cut(s) 104, 1008, 1224, 1334
MspR9I CCNGG 5 cut(s) 105, 929, 1009, 1334, 1413
MunI CAATTG 1 cut(s) 1346
Mva1269I GAATGC 1 cut(s) 1025
MvaI CCWGG 2 cut(s) 929, 1413
MwoI GCNNNNNNNGC 4 cut(s) 544, 690, 1009, 1573
NciI CCSGG 3 cut(s) 105, 1009, 1334
NdeII GATC 5 cut(s) 190, 205, 361, 1338, 1541
NlaIII CATG 6 cut(s) 206, 314, 338, 487, 645, 1111
NlaIV GGNNCC 5 cut(s) 468, 970, 1313, 1327, 1543
NspI RCATGY 1 cut(s) 314
PagI TCATGA 2 cut(s) 202, 483
PaqCI CACCTGC 1 cut(s) 1506
PciSI GCTCTTC 1 cut(s) 167
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PctI GAATGC 1 cut(s) 1025
PdmI GAANNNNTTC 1 cut(s) 1316
PfeI GAWTC 3 cut(s) 1033, 1276, 1582
PflMI CCANNNNNTGG 1 cut(s) 1100
PfoI TCCNGGA 1 cut(s) 1332
PkrI GCNGC 4 cut(s) 82, 751, 1451, 1526
PleI GAGTC 2 cut(s) 135, 1357
PmaCI CACGTG 1 cut(s) 100
PmlI CACGTG 1 cut(s) 100
PpsI GAGTC 2 cut(s) 135, 1357
Ppu21I YACGTR 2 cut(s) 100, 124
Psp6I CCWGG 2 cut(s) 927, 1411
PspCI CACGTG 1 cut(s) 100
PspGI CCWGG 2 cut(s) 927, 1411
PspN4I GGNNCC 5 cut(s) 468, 970, 1313, 1327, 1543
PspPI GGNCC 3 cut(s) 824, 1010, 1516
PstI CTGCAG 1 cut(s) 751
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 2 cut(s) 1338, 1541
PvuII CAGCTG 1 cut(s) 985
RsaI GTAC 3 cut(s) 122, 1056, 1511
RsaNI GTAC 3 cut(s) 121, 1055, 1510
RseI CAYNNNNRTG 1 cut(s) 11
SalI GTCGAC 1 cut(s) 110
SapI GCTCTTC 1 cut(s) 167
SaqAI TTAA 5 cut(s) 35, 1430, 1553, 1587, 1663
SatI GCNGC 4 cut(s) 81, 750, 1450, 1525
Sau3AI GATC 5 cut(s) 190, 205, 361, 1338, 1541
Sau96I GGNCC 3 cut(s) 824, 1010, 1516
SchI GAGTC 2 cut(s) 135, 1357
ScrFI CCNGG 5 cut(s) 105, 929, 1009, 1334, 1413
SduI GDGCHC 2 cut(s) 129, 477
SfaNI GCATC 2 cut(s) 313, 399
SfcI CTRYAG 1 cut(s) 747
SinI GGWCC 1 cut(s) 1516
SmiMI CAYNNNNRTG 1 cut(s) 11
SsiI CCGC 2 cut(s) 1450, 1501
SspI AATATT 1 cut(s) 1231
SspMI CTAG 2 cut(s) 209, 1236
StyD4I CCNGG 5 cut(s) 103, 927, 1007, 1332, 1411
TaaI ACNGT 2 cut(s) 712, 959
TaiI ACGT 5 cut(s) 102, 126, 132, 239, 566
TaqI TCGA 3 cut(s) 111, 771, 1368
TatI WGTACW 1 cut(s) 1054
TauI GCSGC 1 cut(s) 1452
TfiI GAWTC 3 cut(s) 1033, 1276, 1582
Tru1I TTAA 5 cut(s) 35, 1430, 1553, 1587, 1663
Tru9I TTAA 5 cut(s) 35, 1430, 1553, 1587, 1663
TscAI CASTG 3 cut(s) 751, 970, 1084
TseI GCWGC 3 cut(s) 80, 749, 1524
TspDTI ATGAA 8 cut(s) 29, 158, 390, 446, 500, 630, 1104, 1521
TspRI CASTG 3 cut(s) 751, 970, 1084
Van91I CCANNNNNTGG 1 cut(s) 1100
VneI GTGCAC 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 1516
XapI RAATTY 3 cut(s) 637, 720, 1257
XceI RCATGY 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 1 cut(s) 1316
XspI CTAG 2 cut(s) 209, 1236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.