MD05G1182000.v1.1

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000965
Physical Location & Seq
Forward (+)
31037360 .. 31037815
456 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1182000.v1.1.491

Sequence Viewer

Length: 456 bp
ATGAATGATTTCAATCGTCATGCGTTTCTCTTTGTGTGTGCAGCACTAGCCCTAAGTGCTCCATGCCTAGCAAATTTCCATGAAGACAATTGGGAGCTTCGTCCTGGTAGATGGCATGTACACGTGGTAAATAACTTGGGAGCCGGAAAAACATTGTTCGCCCATTGTCAGTCCAAGAATGACGATATCGGAAACCGTACCATAGCACCTGGGGCTGAGATCACTTGGAGCTTCAAGCCGAACTTCTTCGGGACGACGCTGTATTGGTGTTACACGCGCACGGACCATGAGCATACGGCGTTCGATGTGTATTGGGAGGAGAGTAAACATAATTGGCTGCGTTACAGATGCAACTATAAGGAATGCTTTTGGATTGCAAAGGATGATGGGTTTTATATACGAGTTATTCCTGAAAAACGTGATGAGTTGATTCACAAATGGGAGCCGGGGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

152

Amino Acids

17.99

Weight (kDa)

6.49

Isoelectric Point (pI)

33.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 39 - 147 2.6e-27 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 277
AcsI RAATTY 1 cut(s) 73
AcvI CACGTG 1 cut(s) 124
AfaI GTAC 2 cut(s) 120, 199
AflIII ACRYGT 1 cut(s) 121
AgsI TTSAA 2 cut(s) 13, 235
AjnI CCWGG 2 cut(s) 103, 208
AluBI AGCT 2 cut(s) 97, 231
AluI AGCT 2 cut(s) 97, 231
Alw21I GWGCWC 1 cut(s) 61
ApeKI GCWGC 2 cut(s) 41, 337
ApoI RAATTY 1 cut(s) 73
Asp700I GAANNNNTTC 2 cut(s) 8, 245
AspLEI GCGC 1 cut(s) 279
AspS9I GGNCC 1 cut(s) 283
AsuC2I CCSGG 1 cut(s) 447
AvaII GGWCC 1 cut(s) 283
BbrPI CACGTG 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 61
BbvI GCAGC 2 cut(s) 53, 324
BccI CCATC 2 cut(s) 105, 380
BceAI ACGGC 1 cut(s) 312
BciT130I CCWGG 2 cut(s) 105, 210
BcnI CCSGG 1 cut(s) 447
BfaI CTAG 2 cut(s) 47, 68
BisI GCNGC 2 cut(s) 42, 338
BlsI GCNGC 2 cut(s) 43, 339
Bme1390I CCNGG 3 cut(s) 105, 210, 447
Bme18I GGWCC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 2 cut(s) 142, 444
BmrFI CCNGG 3 cut(s) 105, 210, 447
BmsI GCATC 1 cut(s) 338
BpiI GAAGAC 1 cut(s) 90
BpuMI CCSGG 1 cut(s) 447
BsaAI YACGTR 1 cut(s) 124
BsaBI GATNNNNATC 1 cut(s) 12
BsaJI CCNNGG 2 cut(s) 209, 446
BsaXI ACNNNNNCTCC 1 cut(s) 434
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 2 cut(s) 105, 210
BseDI CCNNGG 2 cut(s) 209, 446
BseGI GGATG 1 cut(s) 388
BseJI GATNNNNATC 1 cut(s) 12
BseMII CTCAG 1 cut(s) 207
BseRI GAGGAG 1 cut(s) 332
BseXI GCAGC 2 cut(s) 53, 324
BsgI GTGCAG 1 cut(s) 60
Bsh1236I CGCG 1 cut(s) 277
BsiHKAI GWGCWC 1 cut(s) 61
BsiSI CCGG 2 cut(s) 144, 446
BslFI GGGAC 1 cut(s) 265
BsmFI GGGAC 1 cut(s) 265
BsmI GAATGC 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 61
Bsp1407I TGTACA 1 cut(s) 118
Bsp143I GATC 1 cut(s) 219
BspCNI CTCAG 1 cut(s) 208
BspFNI CGCG 1 cut(s) 277
BspLI GGNNCC 2 cut(s) 142, 444
BsrGI TGTACA 1 cut(s) 118
BssECI CCNNGG 2 cut(s) 209, 446
BssMI GATC 1 cut(s) 219
Bst2UI CCWGG 2 cut(s) 105, 210
Bst4CI ACNGT 1 cut(s) 197
BstAUI TGTACA 1 cut(s) 118
BstBAI YACGTR 1 cut(s) 124
BstDEI CTNAG 2 cut(s) 53, 216
BstF5I GGATG 1 cut(s) 388
BstFNI CGCG 1 cut(s) 277
BstHHI GCGC 1 cut(s) 279
BstKTI GATC 1 cut(s) 222
BstMBI GATC 1 cut(s) 219
BstMWI GCNNNNNNNGC 3 cut(s) 47, 56, 212
BstNI CCWGG 2 cut(s) 105, 210
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 3 cut(s) 103, 208, 445
BstUI CGCG 1 cut(s) 277
BstV1I GCAGC 2 cut(s) 53, 324
BstV2I GAAGAC 1 cut(s) 90
BtsCI GGATG 1 cut(s) 388
CfoI GCGC 1 cut(s) 279
Cfr13I GGNCC 1 cut(s) 283
CseI GACGC 1 cut(s) 265
Csp6I GTAC 2 cut(s) 119, 198
CviAII CATG 5 cut(s) 20, 63, 80, 116, 287
CviJI RGCY 8 cut(s) 50, 97, 143, 215, 231, 238, 337, 445
CviKI_1 RGCY 8 cut(s) 50, 97, 143, 215, 231, 238, 337, 445
CviQI GTAC 2 cut(s) 119, 198
DdeI CTNAG 2 cut(s) 53, 216
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 283
Eco72I CACGTG 1 cut(s) 124
EcoRII CCWGG 2 cut(s) 103, 208
EcoRV GATATC 1 cut(s) 187
FaeI CATG 5 cut(s) 23, 66, 83, 119, 290
FalI AAGNNNNNCTT 4 cut(s) 227, 259, 350, 382
FaqI GGGAC 1 cut(s) 265
FatI CATG 5 cut(s) 19, 62, 79, 115, 286
Fnu4HI GCNGC 2 cut(s) 42, 338
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 2 cut(s) 42, 338
FspBI CTAG 2 cut(s) 47, 68
GlaI GCGC 1 cut(s) 278
GluI GCNGC 2 cut(s) 42, 338
HapII CCGG 2 cut(s) 144, 446
HgaI GACGC 1 cut(s) 265
HhaI GCGC 1 cut(s) 279
Hin1II CATG 5 cut(s) 23, 66, 83, 119, 290
Hin6I GCGC 1 cut(s) 277
HinP1I GCGC 1 cut(s) 277
HinfI GANTC 1 cut(s) 430
HpaII CCGG 2 cut(s) 144, 446
Hpy166II GTNNAC 2 cut(s) 121, 326
Hpy188I TCNGA 1 cut(s) 191
Hpy188III TCNNGA 2 cut(s) 250, 410
Hpy8I GTNNAC 2 cut(s) 121, 326
Hpy99I CGWCG 1 cut(s) 259
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4IV ACGT 2 cut(s) 123, 418
HpyCH4V TGCA 3 cut(s) 41, 351, 377
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 56, 212
HpyF3I CTNAG 2 cut(s) 53, 216
HpySE526I ACGT 2 cut(s) 123, 418
Hsp92II CATG 5 cut(s) 23, 66, 83, 119, 290
HspAI GCGC 1 cut(s) 277
Kzo9I GATC 1 cut(s) 219
LmnI GCTCC 5 cut(s) 64, 94, 140, 228, 442
LpnPI CCDG 6 cut(s) 90, 117, 157, 195, 222, 423
Lsp1109I GCAGC 2 cut(s) 53, 324
LweI GCATC 1 cut(s) 338
MaeI CTAG 2 cut(s) 47, 68
MaeII ACGT 2 cut(s) 123, 418
MaeIII GTNAC 2 cut(s) 269, 341
MalI GATC 1 cut(s) 221
MboI GATC 1 cut(s) 219
MboII GAAGA 2 cut(s) 95, 238
MfeI CAATTG 1 cut(s) 88
MhlI GDGCHC 1 cut(s) 61
MluCI AATT 3 cut(s) 73, 88, 331
MnlI CCTC 1 cut(s) 310
MroXI GAANNNNTTC 2 cut(s) 8, 245
MspI CCGG 2 cut(s) 144, 446
MspR9I CCNGG 3 cut(s) 105, 210, 447
MunI CAATTG 1 cut(s) 88
Mva1269I GAATGC 1 cut(s) 368
MvaI CCWGG 2 cut(s) 105, 210
MvnI CGCG 1 cut(s) 277
MwoI GCNNNNNNNGC 3 cut(s) 47, 56, 212
NciI CCSGG 1 cut(s) 447
NdeII GATC 1 cut(s) 219
NlaIII CATG 5 cut(s) 23, 66, 83, 119, 290
NlaIV GGNNCC 2 cut(s) 142, 444
NspI RCATGY 1 cut(s) 119
PctI GAATGC 1 cut(s) 368
PdmI GAANNNNTTC 2 cut(s) 8, 245
PfeI GAWTC 1 cut(s) 430
PkrI GCNGC 2 cut(s) 43, 339
PmaCI CACGTG 1 cut(s) 124
PmlI CACGTG 1 cut(s) 124
Ppu21I YACGTR 1 cut(s) 124
Psp6I CCWGG 2 cut(s) 103, 208
PspCI CACGTG 1 cut(s) 124
PspGI CCWGG 2 cut(s) 103, 208
PspN4I GGNNCC 2 cut(s) 142, 444
PspPI GGNCC 1 cut(s) 283
RsaI GTAC 2 cut(s) 120, 199
RsaNI GTAC 2 cut(s) 119, 198
SatI GCNGC 2 cut(s) 42, 338
Sau3AI GATC 1 cut(s) 219
Sau96I GGNCC 1 cut(s) 283
ScrFI CCNGG 3 cut(s) 105, 210, 447
SduI GDGCHC 1 cut(s) 61
SetI ASST 5 cut(s) 99, 126, 211, 233, 421
SfaNI GCATC 1 cut(s) 338
SinI GGWCC 1 cut(s) 283
Sse9I AATT 3 cut(s) 73, 88, 331
SspMI CTAG 2 cut(s) 47, 68
StyD4I CCNGG 3 cut(s) 103, 208, 445
TaaI ACNGT 1 cut(s) 197
TaiI ACGT 2 cut(s) 126, 421
TaqI TCGA 1 cut(s) 303
TasI AATT 3 cut(s) 73, 88, 331
TatI WGTACW 1 cut(s) 118
TfiI GAWTC 1 cut(s) 430
TseI GCWGC 2 cut(s) 41, 337
TspDTI ATGAA 2 cut(s) 17, 96
TspGWI ACGGA 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 283
XapI RAATTY 1 cut(s) 73
XceI RCATGY 1 cut(s) 119
XmnI GAANNNNTTC 2 cut(s) 8, 245
XspI CTAG 2 cut(s) 47, 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.