FvH4_7g24042

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001015
Physical Location & Seq
Forward (+)
18617688 .. 18618557
870 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24042.t1

Sequence Viewer

Length: 582 bp
ATGAAGAGAAGCTATGACGACAACCATCGCTCTCCAAAAGCAACCTCGAACAACAACACCAGCCGTAAAAAGTGCAGAGCAACGGCTGAGATGAAGAGGAAAAGGATCATACGACAAAGAAGATTTCACGGTGTAGATTTGTTTCTTGAGCTCGACGGAGTCGTCCTACTTGACCCCCCAACTTCTGATCAGTGCTACCAAGTGGTGTGGTGGATTGAGCCCGACGGAGAAAAGCACATTTCTGACAAGGTTACAGGGTCACCGAACCCTAAATGGGACAAAAAGTGGTACGTTGGATTAAAGGGAAACCCTGTGGGTGAGGACCTCTTTGTAAACTTGGAGGTTTTGAGATATAACAGCACGAAAAACTGCTGCGACCCCGGAACCTCCGACGATCCCAGCATAGGGGAAGTAATTCGGGCTAGGGTTCCGTTTCCGAAGAGGCTCGAAGATACAATACAAGGGAGGTATGAACTTGTGAAGCCTGATGGAGCCGGGAACATGAGGATCGCTGGTGATATAGACTTGACGATGTCACTGGAGACATGGACCAAAGACTATTACAGATGGACGTTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

194

Amino Acids

22.3

Weight (kDa)

8.82

Isoelectric Point (pI)

37.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
AclWI GGATC 3 cut(s) 113, 389, 515
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 3 cut(s) 274, 404, 405
AluBI AGCT 2 cut(s) 12, 151
AluI AGCT 2 cut(s) 12, 151
Alw21I GWGCWC 1 cut(s) 153
Alw26I GTCTC 1 cut(s) 536
AlwI GGATC 3 cut(s) 113, 389, 515
ApeKI GCWGC 1 cut(s) 372
Asp700I GAANNNNTTC 1 cut(s) 414
AspS9I GGNCC 2 cut(s) 322, 549
AsuC2I CCSGG 2 cut(s) 381, 496
AsuHPI GGTGA 3 cut(s) 252, 329, 527
AvaII GGWCC 2 cut(s) 322, 549
BanII GRGCYC 2 cut(s) 153, 222
BarI GAAGNNNNNNTAC 2 cut(s) 441, 473
Bbv12I GWGCWC 1 cut(s) 153
BbvI GCAGC 1 cut(s) 359
BccI CCATC 3 cut(s) 33, 482, 561
BceAI ACGGC 2 cut(s) 48, 99
BclI TGATCA 1 cut(s) 187
BcnI CCSGG 2 cut(s) 381, 496
BcoDI GTCTC 1 cut(s) 536
BfaI CTAG 1 cut(s) 423
BisI GCNGC 1 cut(s) 373
BlsI GCNGC 1 cut(s) 374
Bme1390I CCNGG 2 cut(s) 381, 496
Bme18I GGWCC 2 cut(s) 322, 549
BmgT120I GGNCC 2 cut(s) 322, 549
BmiI GGNNCC 3 cut(s) 385, 429, 493
BmrFI CCNGG 2 cut(s) 381, 496
BpmI CTGGAG 1 cut(s) 560
BpuEI CTTGAG 1 cut(s) 167
BpuMI CCSGG 2 cut(s) 381, 496
BsaJI CCNNGG 1 cut(s) 379
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 404, 405
Bse1I ACTGG 1 cut(s) 543
BseDI CCNNGG 1 cut(s) 379
BseLI CCNNNNNNNGG 3 cut(s) 274, 404, 405
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 1 cut(s) 543
BseXI GCAGC 1 cut(s) 359
BseYI CCCAGC 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 153
BsiSI CCGG 2 cut(s) 381, 495
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 3 cut(s) 274, 404, 405
BsmAI GTCTC 1 cut(s) 536
BsmFI GGGAC 1 cut(s) 290
Bsp1286I GDGCHC 2 cut(s) 153, 222
Bsp143I GATC 4 cut(s) 105, 187, 394, 507
BspCNI CTCAG 1 cut(s) 79
BspLI GGNNCC 3 cut(s) 385, 429, 493
BspPI GGATC 3 cut(s) 113, 389, 515
BsrI ACTGG 1 cut(s) 543
BssECI CCNNGG 1 cut(s) 379
BssMI GATC 4 cut(s) 105, 187, 394, 507
Bst4CI ACNGT 1 cut(s) 131
Bst6I CTCTTC 2 cut(s) 89, 434
BstDEI CTNAG 1 cut(s) 87
BstEII GGTNACC 1 cut(s) 258
BstKTI GATC 4 cut(s) 108, 190, 397, 510
BstMAI GTCTC 1 cut(s) 536
BstMBI GATC 4 cut(s) 105, 187, 394, 507
BstPI GGTNACC 1 cut(s) 258
BstSCI CCNGG 2 cut(s) 379, 494
BstV1I GCAGC 1 cut(s) 359
BtgZI GCGATG 1 cut(s) 11
BtsIMutI CAGTG 2 cut(s) 197, 536
Cfr13I GGNCC 2 cut(s) 322, 549
Csp6I GTAC 1 cut(s) 289
CspCI CAANNNNNGTGG 2 cut(s) 188, 223
CviAII CATG 2 cut(s) 502, 546
CviJI RGCY 9 cut(s) 12, 63, 86, 151, 220, 422, 445, 484, 494
CviKI_1 RGCY 9 cut(s) 12, 63, 86, 151, 220, 422, 445, 484, 494
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 1 cut(s) 87
DpnI GATC 4 cut(s) 107, 189, 396, 509
DpnII GATC 4 cut(s) 105, 187, 394, 507
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
Eam1104I CTCTTC 2 cut(s) 89, 434
EarI CTCTTC 2 cut(s) 89, 434
Ecl136II GAGCTC 1 cut(s) 151
Eco24I GRGCYC 2 cut(s) 153, 222
Eco47I GGWCC 2 cut(s) 322, 549
Eco53kI GAGCTC 1 cut(s) 151
Eco91I GGTNACC 1 cut(s) 258
EcoICRI GAGCTC 1 cut(s) 151
EcoO109I RGGNCCY 1 cut(s) 322
EcoO65I GGTNACC 1 cut(s) 258
EcoT38I GRGCYC 2 cut(s) 153, 222
FaeI CATG 2 cut(s) 505, 549
FaiI YATR 8 cut(s) 15, 110, 354, 404, 471, 503, 521, 547
FaqI GGGAC 1 cut(s) 290
FatI CATG 2 cut(s) 501, 545
FbaI TGATCA 1 cut(s) 187
Fnu4HI GCNGC 1 cut(s) 373
FriOI GRGCYC 2 cut(s) 153, 222
Fsp4HI GCNGC 1 cut(s) 373
FspBI CTAG 1 cut(s) 423
GluI GCNGC 1 cut(s) 373
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 1 cut(s) 560
HapII CCGG 2 cut(s) 381, 495
Hin1II CATG 2 cut(s) 505, 549
HinfI GANTC 1 cut(s) 159
HpaII CCGG 2 cut(s) 381, 495
HphI GGTGA 3 cut(s) 252, 329, 527
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 4 cut(s) 187, 244, 391, 438
Hpy188III TCNNGA 2 cut(s) 146, 579
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 3 cut(s) 158, 227, 395
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4IV ACGT 2 cut(s) 291, 572
HpyCH4V TGCA 1 cut(s) 75
HpyF3I CTNAG 1 cut(s) 87
HpySE526I ACGT 2 cut(s) 291, 572
Hsp92II CATG 2 cut(s) 505, 549
Ksp22I TGATCA 1 cut(s) 187
Kzo9I GATC 4 cut(s) 105, 187, 394, 507
LmnI GCTCC 1 cut(s) 491
LpnPI CCDG 9 cut(s) 73, 240, 324, 394, 412, 498, 498, 508, 524
Lsp1109I GCAGC 1 cut(s) 359
MaeI CTAG 1 cut(s) 423
MaeII ACGT 2 cut(s) 291, 572
MaeIII GTNAC 3 cut(s) 250, 258, 534
MalI GATC 4 cut(s) 107, 189, 396, 509
MboI GATC 4 cut(s) 105, 187, 394, 507
MboII GAAGA 5 cut(s) 16, 106, 132, 451, 461
MhlI GDGCHC 2 cut(s) 153, 222
MluCI AATT 1 cut(s) 414
MlyI GAGTC 1 cut(s) 168
MmeI TCCRAC 2 cut(s) 274, 414
MnlI CCTC 9 cut(s) 55, 90, 313, 334, 335, 397, 435, 459, 498
MroXI GAANNNNTTC 1 cut(s) 414
MseI TTAA 1 cut(s) 299
MspI CCGG 2 cut(s) 381, 495
MspR9I CCNGG 2 cut(s) 381, 496
NciI CCSGG 2 cut(s) 381, 496
NdeII GATC 4 cut(s) 105, 187, 394, 507
NlaIII CATG 2 cut(s) 505, 549
NlaIV GGNNCC 3 cut(s) 385, 429, 493
NmuCI GTSAC 2 cut(s) 258, 534
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PdmI GAANNNNTTC 1 cut(s) 414
PflFI GACNNNGTC 2 cut(s) 158, 532
PkrI GCNGC 1 cut(s) 374
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
PpuMI RGGWCCY 1 cut(s) 322
Psp124BI GAGCTC 1 cut(s) 153
Psp5II RGGWCCY 1 cut(s) 322
PspEI GGTNACC 1 cut(s) 258
PspFI CCCAGC 1 cut(s) 398
PspN4I GGNNCC 3 cut(s) 385, 429, 493
PspPI GGNCC 2 cut(s) 322, 549
PspPPI RGGWCCY 1 cut(s) 322
PsyI GACNNNGTC 2 cut(s) 158, 532
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SacI GAGCTC 1 cut(s) 153
SaqAI TTAA 1 cut(s) 299
SatI GCNGC 1 cut(s) 373
Sau3AI GATC 4 cut(s) 105, 187, 394, 507
Sau96I GGNCC 2 cut(s) 322, 549
SchI GAGTC 1 cut(s) 168
ScrFI CCNGG 2 cut(s) 381, 496
SduI GDGCHC 2 cut(s) 153, 222
SinI GGWCC 2 cut(s) 322, 549
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 1 cut(s) 414
SspMI CTAG 1 cut(s) 423
SstI GAGCTC 1 cut(s) 153
StyD4I CCNGG 2 cut(s) 379, 494
TaaI ACNGT 1 cut(s) 131
TaiI ACGT 2 cut(s) 294, 575
TaqI TCGA 3 cut(s) 47, 153, 447
TasI AATT 1 cut(s) 414
Tru1I TTAA 1 cut(s) 299
Tru9I TTAA 1 cut(s) 299
TscAI CASTG 2 cut(s) 197, 543
TseFI GTSAC 2 cut(s) 258, 534
TseI GCWGC 1 cut(s) 372
Tsp45I GTSAC 2 cut(s) 258, 534
TspDTI ATGAA 3 cut(s) 17, 107, 486
TspGWI ACGGA 3 cut(s) 171, 240, 420
TspRI CASTG 2 cut(s) 197, 543
Tth111I GACNNNGTC 2 cut(s) 158, 532
VpaK11BI GGWCC 2 cut(s) 322, 549
XmnI GAANNNNTTC 1 cut(s) 414
XspI CTAG 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.